Restriction map for
pFN21AE2150
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# NO CUT ENZYMES ( 170 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AfaI AflII
AflIII AgeI AhdI Alw44I ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BanI
BbeI BbsI BbvCI BcgI BciVI
BcnI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaHI BsaI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsmBI BsmFI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Cac8I CelII Cfr10I
ClaI CpoI DraI DraII DraIII
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoNI EcoO109I EcoO65I EcoRV
EcoT22I FauI FseI FspI HaeIII
HgaI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeIII
MfeI MluI MluNI MroI MscI
MspA1I MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIII NlaIV NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmeI PpuMI PshAI
PshBI Psp1406I PvuI PvuII RcaI
RsaI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
TaqI Tsp45I Tth111I TthHB8I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AlwNI 463 CAGAATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 53 CCTCAGG CC^T[ACGT]AGG
Aor51HI 602 AGCGCT AGC^GCT
AvaII 670 GGTCC G^G[AT]CC
BbrPI 578 CACGTG CAC^GTG
BclI 537 TGATCA T^GATCA
BfaI 404 CTAG C^TAG
BpmI 306 CTGGAG CTGGAG(16/14)
BsaAI 578 CACGTG [CT]AC^GT[GA]
BsaBI 696 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 118 ACCGGA [AT]^CCGG[AT]
BsmAI 298 GAGAC GTCTC(1/5)
BspMI 419 ACCTGC ACCTGC(4/8)
BsrDI 528 GCAATG GCAATG(2/0)
BsrI 587 CCAGT ACTGG(1/-1)
BssKI 305 CCTGG ^CC[ACGT]GG
BstF5I 574 CATCC GGATG(2/0)
BstNI 305 CCTGG CC^[AT]GG
BstXI 319 CCAAAGAATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 53 CCTCAGG CC^T[ACGT]AGG
CfoI 603 GCGC GCG^C
Cfr13I 670 GGTCC G^G[ACGT]CC
DsaI 290 CCGTGG C^C[GA][CT]GG
Eco47III 602 AGCGCT AGC^GCT
Eco81I 53 CCTCAGG CC^T[ACGT]AGG
EcoRI 43 GAATTC G^AATTC
EcoRII 305 CCTGG ^CC[AT]GG
EcoT14I 86 CCAAGG C^C[AT][AT]GG
FbaI 537 TGATCA T^GATCA
FokI 574 CATCC GGATG(9/13)
HaeII 602 AGCGCT [GA]GCGC^[CT]
HapII 119 CCGG C^CGG
HhaI 603 GCGC GCG^C
HinP1I 603 GCGC G^CGC
HpaII 119 CCGG C^CGG
MaeI 404 CTAG C^TAG
MaeII 579 ACGT A^CGT
MamI 696 GATGAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 659 TTAA T^TAA
MspI 119 CCGG C^CGG
MvaI 305 CCTGG CC^[AT]GG
PmaCI 578 CACGTG CAC^GTG
PmlI 578 CACGTG CAC^GTG
PstI 77 CTGCAG CTGCA^G
SapI 442 GAAGAGC GCTCTTC(1/4)
Sau96I 670 GGTCC G^G[ACGT]CC
ScrFI 305 CCTGG CC^[ACGT]GG
SfcI 77 CTGCAG C^T[GA][CT]AG
SmlI 437 CTCAAG C^T[CT][GA]AG
SspI 212 AATATT AAT^ATT
StyI 86 CCAAGG C^C[AT][AT]GG
Tru9I 659 TTAA T^TAA
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# TWO CUT ENZYMES ( 13 enzymes) #
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AcsI 43 GAATTC [GA]^AATT[CT]
618 AAATTT
ApoI 43 GAATTC [GA]^AATT[CT]
618 AAATTT
BanII 496 GGGCTC G[GA]GC[CT]^C
583 GAGCCC
BmyI 496 GGGCTC G[AGT]GC[ACT]^C
583 GAGCCC
BsaJI 86 CCAAGG C^C[ACGT][ACGT]GG
290 CCGTGG
Bsp1286I 496 GGGCTC G[AGT]GC[ACT]^C
583 GAGCCC
BstYI 504 AGATCC [GA]^GATC[CT]
701 AGATCC
MflI 504 AGATCC [GA]^GATC[CT]
701 AGATCC
MslI 263 CATTGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
574 CATCCACGTG
MwoI 70 GCCTCTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
519 GCGACATTGGC
PflMI 563 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
635 CCAAAAGATGG
Van91I 563 CCACATTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
635 CCAAAAGATGG
XhoII 504 AGATCC [GA]^GATC[CT]
701 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
#########################################
AluI AlwI BbvI BsiYI BslI
DdeI DpnII EarI Fnu4HI HinfI
HphI ItaI Ksp632I MboI MboII
MnlI NdeII Sau3AI SfaNI TfiI
TseI Tsp509I TspRI