(Circular) (Six-base) MAPSORT of: pFN21AE2150.seq Check: 6855 from: 1 to: 5416 pFN21AE2150 5416 bp With 182 enzymes: SgfI * July 6, 2012 17:36 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2433 2433 Size: 5416 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3226 278 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2759 1937 Size: 822 4594 Acc65I G'GTAC_C Cuts at: 2737 2737 Size: 5416 AclI AA'CG_TT Cuts at: 1563 3543 3916 1563 Size: 1980 373 3063 Fragments arranged by size: 3063 1980 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2049 3479 4863 5131 1632 Size: 417 1430 1384 268 1917 Fragments arranged by size: 1917 1430 1384 417 268 AfeI AGC'GCT Cuts at: 2609 2609 Size: 5416 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5219 AflIII A'CryG_T Cuts at: 1203 4315 5292 1203 Size: 3112 977 1327 Fragments arranged by size: 3112 1327 977 AgeI A'CCGG_T Cuts at: 4247 4247 Size: 5416 AhdI GACnn_n'nnGTC Cuts at: 4145 4145 Size: 5416 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5416 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5416 AlwNI CAG_nnn'CTG Cuts at: 2473 3159 4731 2473 Size: 686 1572 3158 Fragments arranged by size: 3158 1572 686 ApaLI G'TGCA_C Cuts at: 3473 4629 3473 Size: 1156 4260 ApoI r'AATT_y Cuts at: 1493 2048 2623 2725 2951 3103 5212 1493 Size: 555 575 102 226 152 2109 1697 Fragments arranged by size: 2109 1697 575 555 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 3970 160 Size: 872 2938 1606 Fragments arranged by size: 2938 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 AvaI C'yCGr_G Cuts at: 1870 1943 2741 1870 Size: 73 798 4545 Fragments arranged by size: 4545 798 73 BamHI G'GATC_C Cuts at: 2746 3113 2746 Size: 367 5049 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2737 4192 618 Size: 325 217 627 950 1455 1842 Fragments arranged by size: 1842 1455 950 627 325 217 BanII G_rGCy'C Cuts at: 1391 2505 2592 2735 1391 Size: 1114 87 143 4072 Fragments arranged by size: 4072 1114 143 87 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4445 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3607 3641 1903 Size: 34 1670 34 3678 Fragments arranged by size: 3678 1670 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3308 4518 3308 Size: 1210 4206 BclI T'GATC_A Cuts at: 1548 2542 1548 Size: 994 4422 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4027 136 Size: 107 122 71 631 605 2355 1525 Fragments arranged by size: 2355 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5411 5411 Size: 5416 BlpI GC'TnA_GC Cuts at: 2833 2833 Size: 5416 Bme1580I G_kGCm'C Cuts at: 1790 3477 4633 1790 Size: 1687 1156 2573 Fragments arranged by size: 2573 1687 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5416 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2586 3147 4105 Size: 416 53 83 399 24 1169 561 958 Cuts at: 4105 442 Size: 1753 Fragments arranged by size: 1753 1169 958 561 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5416 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2332 4076 1239 Size: 1093 1744 2579 Fragments arranged by size: 2579 1744 1093 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2427 3541 4406 4704 2427 Size: 1114 865 298 3139 Fragments arranged by size: 3139 1114 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4079 882 Size: 617 2580 2219 Fragments arranged by size: 2580 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2585 3207 493 Size: 711 1381 622 2702 Fragments arranged by size: 2702 1381 711 622 BsaBI GATnn'nnATC Cuts at: 2705 5410 2705 Size: 2705 2711 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3223 3605 4240 275 Size: 53 83 186 936 1690 382 635 1451 Fragments arranged by size: 1690 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2123 3849 4247 4521 4668 5079 2123 Size: 1726 398 274 147 411 2460 Fragments arranged by size: 2460 1726 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5191 Fragments arranged by size: 5191 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5416 BseYI C'CCAG_C Cuts at: 4619 5115 4619 Size: 496 4920 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4843 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2866 3627 4655 1540 Size: 275 186 865 761 1028 2301 Fragments arranged by size: 2301 1028 865 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3477 3562 4633 3477 Size: 85 1071 4260 Fragments arranged by size: 4260 1071 85 BsmI GAATG_Cn' Cuts at: 2927 3020 2927 Size: 93 5323 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2505 2592 2735 3477 3562 4633 1391 Size: 399 715 87 143 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 715 399 143 87 85 BspEI T'CCGG_A Cuts at: 5079 5079 Size: 5416 BspHI T'CATG_A Cuts at: 3305 5035 3305 Size: 1730 3686 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2433 2771 844 Size: 1589 338 3489 Fragments arranged by size: 3489 1589 338 BsrBI CCG'CTC Cuts at: 1394 1823 3303 1394 Size: 429 1480 3507 Fragments arranged by size: 3507 1480 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2540 3911 4085 65 Size: 1183 1292 1371 174 1396 Fragments arranged by size: 1396 1371 1292 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4060 4247 1700 Size: 110 2250 187 2869 Fragments arranged by size: 2869 2250 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5416 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5416 BssSI C'ACGA_G Cuts at: 3476 4488 3476 Size: 1012 4404 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5416 BstBI TT'CG_AA Cuts at: 4220 4220 Size: 5416 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5416 BstXI CCAn_nnnn'nTGG Cuts at: 2331 2331 Size: 5416 BstYI r'GATC_y Cuts at: 1972 2509 2706 2746 3113 3498 3515 4967 5053 Size: 537 197 40 367 385 17 1452 86 Cuts at: 5053 5065 5411 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 537 385 367 346 197 86 40 17 12 Bsu36I CC'TnA_GG Cuts at: 2059 2059 Size: 5416 BtgI C'CryG_G Cuts at: 513 1065 2295 513 Size: 552 1230 3634 Fragments arranged by size: 3634 1230 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4677 Fragments arranged by size: 4677 683 56 BtsI GCAGTG_nn' Cuts at: 2932 3726 3746 2932 Size: 794 20 4602 Fragments arranged by size: 4602 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5416 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5381 DraI TTT'AAA Cuts at: 2720 2720 Size: 5416 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4423 809 Size: 507 3107 1802 Fragments arranged by size: 3107 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2863 3752 5381 Size: 54 1425 50 275 18 1033 889 1629 Cuts at: 5381 8 Size: 43 Fragments arranged by size: 1629 1425 1033 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2863 1537 Size: 275 1051 4090 Fragments arranged by size: 4090 1051 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2083 2295 2442 3346 1353 Size: 730 212 147 904 3423 Fragments arranged by size: 3423 904 730 212 147 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4003 4377 4523 387 Size: 1064 138 2414 374 146 1280 Fragments arranged by size: 2414 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2049 2332 3479 4076 4863 5131 1239 Size: 393 417 283 1147 597 787 268 1524 Fragments arranged by size: 1524 1147 787 597 417 393 283 268 EcoRI G'AATT_C Cuts at: 2048 2725 3103 2048 Size: 677 378 4361 Fragments arranged by size: 4361 677 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2728 2728 Size: 5416 FspI TGC'GCA Cuts at: 3922 3922 Size: 5416 HaeII r_GCGC'y Cuts at: 1678 2611 4563 1678 Size: 933 1952 2531 Fragments arranged by size: 2531 1952 933 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 2425 2457 4057 Size: 32 135 32 520 32 301 32 1600 Cuts at: 4057 4089 4131 4163 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1600 520 301 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2760 3006 669 Size: 988 281 822 246 3079 Fragments arranged by size: 3079 988 822 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5416 HpaI GTT'AAC Cuts at: 3006 3006 Size: 5416 KpnI G_GTAC'C Cuts at: 2741 2741 Size: 5416 MfeI C'AATT_G Cuts at: 3015 3015 Size: 5416 MluI A'CGCG_T Cuts at: 5292 5292 Size: 5416 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2373 4530 4714 1305 Size: 1068 2157 184 2007 Fragments arranged by size: 2157 2007 1068 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3594 Fragments arranged by size: 3594 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2272 2583 3374 3733 3892 518 Size: 648 1106 311 791 359 159 2042 Fragments arranged by size: 2042 1106 791 648 359 311 159 MspA1I CmG'CkG Cuts at: 1606 2832 3509 4657 4902 1606 Size: 1226 677 1148 245 2120 Fragments arranged by size: 2120 1226 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4864 NdeI CA'TA_TG Cuts at: 387 387 Size: 5416 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5416 NotI GC'GGCC_GC Cuts at: 2863 2863 Size: 5416 NspI r_CATG'y Cuts at: 1132 1229 2774 4319 1132 Size: 97 1545 1545 2229 Fragments arranged by size: 2229 1545 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5416 PciI A'CATG_T Cuts at: 4315 4315 Size: 5416 PflMI CCAn_nnn'nTGG Cuts at: 1108 2574 2646 1108 Size: 1466 72 3878 Fragments arranged by size: 3878 1466 72 PmeI GTTT'AAAC Cuts at: 2720 2720 Size: 5416 PmlI CAC'GTG Cuts at: 2585 2585 Size: 5416 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3485 3517 4998 5030 3485 Size: 32 1481 32 3871 Fragments arranged by size: 3871 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5416 PsiI TTA'TAA Cuts at: 2986 2986 Size: 5416 PstI C_TGCA'G Cuts at: 830 2086 2768 830 Size: 1256 682 3478 Fragments arranged by size: 3478 1256 682 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5416 SalI G'TCGA_C Cuts at: 1936 2758 1936 Size: 822 4594 SapI GCTCTTCn'nnn_ Cuts at: 2442 2442 Size: 5416 SbfI CC_TGCA'GG Cuts at: 2768 2768 Size: 5416 ScaI AGT'ACT Cuts at: 3664 5376 3664 Size: 1712 3704 SfcI C'TryA_G Cuts at: 826 1045 2082 2764 3899 4580 4771 826 Size: 219 1037 682 1135 681 191 1471 Fragments arranged by size: 1471 1135 1037 682 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5416 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5416 SmaI CCC'GGG Cuts at: 1872 2743 1872 Size: 871 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2442 3520 4421 4683 820 Size: 197 926 499 1078 901 262 1553 Fragments arranged by size: 1553 1078 926 901 499 262 197 SnaBI TAC'GTA Cuts at: 493 3207 493 Size: 2714 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5416 SphI G_CATG'C Cuts at: 1132 2774 1132 Size: 1642 3774 SspI AAT'ATT Cuts at: 5 52 2219 3340 5 Size: 47 2167 1121 2081 Fragments arranged by size: 2167 2081 1121 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5416 StyI C'CwwG_G Cuts at: 513 1065 2091 2855 513 Size: 552 1026 764 3074 Fragments arranged by size: 3074 1026 764 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2653 3443 3460 3613 3798 4243 325 Size: 1255 1073 790 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1073 790 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3662 5374 96 Size: 274 80 33 51 1443 1685 1712 138 Fragments arranged by size: 1712 1685 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5376 5408 5376 Size: 32 5384 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5416 XbaI T'CTAG_A Cuts at: 2752 2752 Size: 5416 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5416 XmaI C'CCGG_G Cuts at: 1870 2741 1870 Size: 871 4545 XmnI GAAnn'nnTTC Cuts at: 3545 3545 Size: 5416 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3224 276 Size: 53 83 186 936 1690 2468 Fragments arranged by size: 2468 1690 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI