Restriction map for
pFN21AE5157
(Circular) (Six-base) MAPSORT of: pFN21AE5157.seq Check: 6309 from: 1 to: 5083
pFN21AE5157 5083 bp
With 182 enzymes: SgfI *
January 30, 2014 17:13 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2893 278
Size: 53 83 186 936 1357 2468
Fragments arranged by size:
2468 1357 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2426 1937
Size: 489 4594
Acc65I G'GTAC_C
Cuts at: 2404 2404
Size: 5083
AclI AA'CG_TT
Cuts at: 1563 3210 3583 1563
Size: 1647 373 3063
Fragments arranged by size:
3063 1647 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2200 2330 3146 4530 4798 1632
Size: 568 130 816 1384 268 1917
Fragments arranged by size:
1917 1384 816 568 268 130
AfeI AGC'GCT
Cuts at: 2041 2041
Size: 5083
AflII C'TTAA_G
Cuts at: 820 1017 2240 820
Size: 197 1223 3663
Fragments arranged by size:
3663 1223 197
AflIII A'CryG_T
Cuts at: 1203 3982 4959 1203
Size: 2779 977 1327
Fragments arranged by size:
2779 1327 977
AgeI A'CCGG_T
Cuts at: 3914 3914
Size: 5083
AhdI GACnn_n'nnGTC
Cuts at: 3812 3812
Size: 5083
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5083
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5083
AlwNI CAG_nnn'CTG
Cuts at: 2826 4398 2826
Size: 1572 3511
ApaLI G'TGCA_C
Cuts at: 3140 4296 3140
Size: 1156 3927
ApoI r'AATT_y
Cuts at: 1493 2392 2618 2770 4879 1493
Size: 899 226 152 2109 1697
Fragments arranged by size:
2109 1697 899 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3637 160
Size: 872 2605 1606
Fragments arranged by size:
2605 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5083
AvaI C'yCGr_G
Cuts at: 1870 1943 2121 2408 1870
Size: 73 178 287 4545
Fragments arranged by size:
4545 287 178 73
BamHI G'GATC_C
Cuts at: 2278 2413 2780 2278
Size: 135 367 4581
Fragments arranged by size:
4581 367 135
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2404 3859 618
Size: 325 217 627 617 1455 1842
Fragments arranged by size:
1842 1455 627 617 325 217
BanII G_rGCy'C
Cuts at: 1391 2402 1391
Size: 1011 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4112
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3274 3308 1903
Size: 34 1337 34 3678
Fragments arranged by size:
3678 1337 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2975 4185 2975
Size: 1210 3873
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5083
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2301 3694 136
Size: 107 122 71 631 605 629 1393 1525
Fragments arranged by size:
1525 1393 631 629 605 122 107 71
BglII A'GATC_T
Cuts at: 5078 5078
Size: 5083
BlpI GC'TnA_GC
Cuts at: 2043 2500 2043
Size: 457 4626
Bme1580I G_kGCm'C
Cuts at: 1790 3144 4300 1790
Size: 1354 1156 2573
Fragments arranged by size:
2573 1354 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5083
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2814 3772 442
Size: 416 53 83 399 24 1397 958 1753
Fragments arranged by size:
1753 1397 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5083
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3743 1239
Size: 2504 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2056 3208 4073 4371 2056
Size: 1152 865 298 2768
Fragments arranged by size:
2768 1152 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3746 882
Size: 617 2247 2219
Fragments arranged by size:
2247 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 2874 493
Size: 711 1670 2702
Fragments arranged by size:
2702 1670 711
BsaBI GATnn'nnATC
Cuts at: 5077 5077
Size: 5083
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2009 2890 3272 3907
Size: 53 83 186 936 476 881 382 635
Cuts at: 3907 275
Size: 1451
Fragments arranged by size:
1451 936 881 635 476 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3516 3914 4188 4335 4746 3516
Size: 398 274 147 411 3853
Fragments arranged by size:
3853 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 4858
Fragments arranged by size:
4858 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5083
BseYI C'CCAG_C
Cuts at: 4286 4782 4286
Size: 496 4587
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4510
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2533 3294 4322 1540
Size: 275 186 532 761 1028 2301
Fragments arranged by size:
2301 1028 761 532 275 186
BsiHKAI G_wGCw'C
Cuts at: 3144 3229 4300 3144
Size: 85 1071 3927
Fragments arranged by size:
3927 1071 85
BsmI GAATG_Cn'
Cuts at: 2104 2594 2687 2104
Size: 490 93 4500
Fragments arranged by size:
4500 490 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2402 3144 3229 4300 1391
Size: 399 612 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 612 399 85
BspEI T'CCGG_A
Cuts at: 4746 4746
Size: 5083
BspHI T'CATG_A
Cuts at: 2972 4702 2972
Size: 1730 3353
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2438 844
Size: 1594 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 2970 1394
Size: 429 1147 3507
Fragments arranged by size:
3507 1147 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3578 3752 65
Size: 1183 2330 174 1396
Fragments arranged by size:
2330 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2026 3727 3914 1700
Size: 110 216 1701 187 2869
Fragments arranged by size:
2869 1701 216 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5083
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5083
BssSI C'ACGA_G
Cuts at: 3143 4155 3143
Size: 1012 4071
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2164 794
Size: 1370 3713
BstBI TT'CG_AA
Cuts at: 3887 3887
Size: 5083
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5083
BstYI r'GATC_y
Cuts at: 1972 2184 2278 2413 2780 3165 3182 4634 4720
Size: 212 94 135 367 385 17 1452 86
Cuts at: 4720 4732 5078 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 385 367 346 212 135 94
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2172 2347 513
Size: 552 939 168 175 3249
Fragments arranged by size:
3249 939 552 175 168
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4344
Fragments arranged by size:
4344 683 56
BtsI GCAGTG_nn'
Cuts at: 2109 2599 3393 3413 2109
Size: 490 794 20 3779
Fragments arranged by size:
3779 794 490 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5083
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5048
DraI TTT'AAA
Cuts at: 2387 2387
Size: 5083
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4090 809
Size: 507 2774 1802
Fragments arranged by size:
2774 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2175 2373 2530
Size: 54 1425 50 275 18 345 198 157
Cuts at: 2530 3419 5048 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 345 275 198 157 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2530 1537
Size: 275 718 4090
Fragments arranged by size:
4090 718 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3013 1353
Size: 1660 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2002 2077 3670 4044 4190 387
Size: 1064 138 413 75 1593 374 146 1280
Fragments arranged by size:
1593 1280 1064 413 374 146 138 75
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2200 2330 3146 3743 4530 4798 1239
Size: 393 568 130 816 597 787 268 1524
Fragments arranged by size:
1524 816 787 597 568 393 268 130
EcoNI CCTnn'n_nnAGG
Cuts at: 2066 2066
Size: 5083
EcoRI G'AATT_C
Cuts at: 2392 2770 2392
Size: 378 4705
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2255 2255
Size: 5083
FspI TGC'GCA
Cuts at: 2108 3589 2108
Size: 1481 3602
FspAI rTGC'GCAy
Cuts at: 2108 2108
Size: 5083
HaeII r_GCGC'y
Cuts at: 1678 2043 2318 4230 1678
Size: 365 275 1912 2531
Fragments arranged by size:
2531 1912 365 275
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3724 3756 3798 3830 1373
Size: 32 135 32 2152 32 42 32 2626
Fragments arranged by size:
2626 2152 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2427 2673 669
Size: 988 281 489 246 3079
Fragments arranged by size:
3079 988 489 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5083
HpaI GTT'AAC
Cuts at: 2673 2673
Size: 5083
KpnI G_GTAC'C
Cuts at: 2408 2408
Size: 5083
MfeI C'AATT_G
Cuts at: 2682 2682
Size: 5083
MluI A'CGCG_T
Cuts at: 4959 4959
Size: 5083
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4197 4381 1305
Size: 2892 184 2007
Fragments arranged by size:
2892 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2177 10
Size: 54 1425 343 345 2916
Fragments arranged by size:
2916 1425 345 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3041 3400 3559 518
Size: 648 1875 359 159 2042
Fragments arranged by size:
2042 1875 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2220 2499 3176 4324 4569 1606
Size: 614 279 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 614 279 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 2347 513
Size: 552 939 343 3249
Fragments arranged by size:
3249 939 552 343
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5083
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5083
NotI GC'GGCC_GC
Cuts at: 2530 2530
Size: 5083
NspI r_CATG'y
Cuts at: 1132 1229 2441 3986 1132
Size: 97 1212 1545 2229
Fragments arranged by size:
2229 1545 1212 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5083
PciI A'CATG_T
Cuts at: 3982 3982
Size: 5083
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2348 1108
Size: 1240 3843
PmeI GTTT'AAAC
Cuts at: 2387 2387
Size: 5083
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3152 3184 4665 4697 3152
Size: 32 1481 32 3538
Fragments arranged by size:
3538 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5083
PsiI TTA'TAA
Cuts at: 2653 2653
Size: 5083
PstI C_TGCA'G
Cuts at: 830 2435 830
Size: 1605 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5083
PvuII CAG'CTG
Cuts at: 2220 2220
Size: 5083
SalI G'TCGA_C
Cuts at: 1936 2425 1936
Size: 489 4594
SbfI CC_TGCA'GG
Cuts at: 2435 2435
Size: 5083
ScaI AGT'ACT
Cuts at: 3331 5043 3331
Size: 1712 3371
SfcI C'TryA_G
Cuts at: 826 1045 2431 3566 4247 4438 826
Size: 219 1386 1135 681 191 1471
Fragments arranged by size:
1471 1386 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5083
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5083
SmaI CCC'GGG
Cuts at: 1872 2410 1872
Size: 538 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2035 2240 3187 4088 4350 820
Size: 197 926 92 205 947 901 262 1553
Fragments arranged by size:
1553 947 926 901 262 205 197 92
SnaBI TAC'GTA
Cuts at: 493 2874 493
Size: 2381 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5083
SphI G_CATG'C
Cuts at: 1132 2441 1132
Size: 1309 3774
SspI AAT'ATT
Cuts at: 5 52 3007 5
Size: 47 2955 2081
Fragments arranged by size:
2955 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5083
StyI C'CwwG_G
Cuts at: 513 1065 2004 2347 2522 513
Size: 552 939 343 175 3074
Fragments arranged by size:
3074 939 552 343 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3110 3127 3280 3465 3910 325
Size: 1255 1530 17 153 185 445 1498
Fragments arranged by size:
1530 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3329 5041 96
Size: 274 80 33 51 1443 1352 1712 138
Fragments arranged by size:
1712 1443 1352 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5043 5075 5043
Size: 32 5051
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5083
XbaI T'CTAG_A
Cuts at: 2419 2419
Size: 5083
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5083
XmaI C'CCGG_G
Cuts at: 1870 2408 1870
Size: 538 4545
XmnI GAAnn'nnTTC
Cuts at: 3212 3212
Size: 5083
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2891 276
Size: 53 83 186 936 1357 2468
Fragments arranged by size:
2468 1357 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoNI EcoRI FalI FspI FspAI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NspI PasI PciI
PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI ApaI AscI AvrII BaeI BbeI BbvCI
BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoO109I EcoRV FseI KasI NaeI NarI
NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI XcmI