################################## # NO CUT ENZYMES ( 148 enzymes) # ################################## AatII AccI AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AvrII BamHI BbeI BbrPI BbvCI BciVI BclI BcnI BfrI BglI BlnI BsaAI BsaBI BsaHI BsaWI BseAI BseRI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstZ17I Bsu36I Cfr10I ClaI CpoI DraI DrdI DsaI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FseI HaeII HapII Hin1I HincII HindII HindIII HpaI HpaII KasI Ksp632I KspI MaeII MamI MfeI MluI MroI MspI MunI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI SspBI SspI SwaI TfiI Tth111I Van91I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### Acc65I 379 GGTACC G^GTACC AccII 35 CGCG CG^CG AlwNI 323 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 379 GGTACC G^GTACC AvaI 65 CTCGAG C^[CT]CG[GA]G BalI 488 TGGCCA TGG^CCA BbvI 575 GCAGC GCAGC(8/12) BcgI 57 GCATCTGGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 535 AGATCT A^GATCT BlpI 5 GCTCAGC GC^T[ACGT]AGC Bpu1102I 5 GCTCAGC GC^T[ACGT]AGC BsaI 248 GAGACC GGTCTC(1/5) BsoBI 65 CTCGAG C^[CT]CG[GA]G BsrBI 198 CCGCTC CCGCTC(-3/-3) BstUI 35 CGCG CG^CG BstXI 113 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 5 GCTCAGC GC^T[ACGT]AGC DraII 518 GGGACCC [GA]G^G[ACGT]CC[CT] DraIII 86 CACCTAGTG CAC[ACGT][ACGT][ACGT]^GTG EaeI 488 TGGCCA [CT]^GGCC[GA] EcoO109I 518 GGGACCC [GA]G^G[ACGT]CC[CT] FauI 197 CCCGC CCCGC(4/6) HgaI 17 GCGTC GACGC(5/10) KpnI 379 GGTACC GGTAC^C MaeIII 25 GTCAC ^GT[ACGT]AC MluNI 488 TGGCCA TGG^CCA MscI 488 TGGCCA TGG^CCA MseI 173 TTAA T^TAA MslI 114 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspA1I 274 CAGCTG C[AC]G^C[GT]G MvnI 35 CGCG CG^CG NspI 177 ACATGC [GA]CATG^[CT] PaeR7I 65 CTCGAG C^TCGAG PpuMI 518 GGGACCC [GA]G^G[AT]CC[CT] PstI 610 CTGCAG CTGCA^G PvuII 274 CAGCTG CAG^CTG SbfI 609 CCTGCAGG CCTGCA^GG SfcI 610 CTGCAG C^T[GA][CT]AG Sse8387I 609 CCTGCAGG CCTGCA^GG StuI 141 AGGCCT AGG^CCT TaqI 66 TCGA T^CGA Tru9I 173 TTAA T^TAA TseI 575 GCAGC G^C[AT]GC Tsp45I 25 GTCAC ^GT[GC]AC Tsp509I 362 AATT ^AATT TthHB8I 66 TCGA T^CGA XhoI 65 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### AfaI 380 GTAC GT^AC 583 GTAC AlwI 193 GATCC GGATC(4/5) 593 GATCC AspHI 126 GAGCTC G[AT]GC[AT]^C 498 GAGCTC AviII 104 TGCGCA TGC^GCA 551 TGCGCA BbsI 234 GAAGAC GAAGAC(2/6) 298 GAAGAC BfaI 89 CTAG C^TAG 586 CTAG BpmI 163 CTGGAG CTGGAG(16/14) 734 CTGGAG BpuAI 234 GAAGAC GAAGAC(2/6) 298 GAAGAC BsiHKAI 126 GAGCTC G[AT]GC[AT]^C 498 GAGCTC BsmAI 248 GAGAC GTCTC(1/5) 526 GAGAC BsmFI 518 GGGAC GGGAC(10/14) 19 GTCCC Cac8I 94 GCTGGC GC[ACGT]^[ACGT]GC 490 GCCAGC FspI 104 TGCGCA TGC^GCA 551 TGCGCA MaeI 89 CTAG C^TAG 586 CTAG RsaI 380 GTAC GT^AC 583 GTAC SacI 126 GAGCTC GAGCT^C 498 GAGCTC SmlI 65 CTCGAG C^T[CT][GA]AG 513 CTCAAG TspRI 243 CAGTG CA[GC]TG[ACGT][ACGT]^ 263 CACTG ######################################### # 3 OR MORE CUT ENZYMES ( 45 enzymes) # ######################################### AciI AluI AvaII BanI BanII BmyI BsaJI BsiYI BslI Bsp1286I BsrI BssKI BstF5I BstNI BstYI CfoI Cfr13I DdeI DpnII EcoRII EcoT14I Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MboI MboII MflI MnlI MvaI MwoI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI StyI XhoII