(Circular) (Six-base) MAPSORT of: pFN21AE6283.seq Check: 9368 from: 1 to: 5509 pFN21AE6283 5509 bp With 182 enzymes: SgfI * March 18, 2015 14:50 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3319 278 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2852 1937 Size: 915 4594 Acc65I G'GTAC_C Cuts at: 2384 2830 2384 Size: 446 5063 AclI AA'CG_TT Cuts at: 1563 3636 4009 1563 Size: 2073 373 3063 Fragments arranged by size: 3063 2073 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 2529 3572 4956 5224 1632 Size: 690 207 1043 1384 268 1917 Fragments arranged by size: 1917 1384 1043 690 268 207 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5312 AflIII A'CryG_T Cuts at: 1203 4408 5385 1203 Size: 3205 977 1327 Fragments arranged by size: 3205 1327 977 AgeI A'CCGG_T Cuts at: 4340 4340 Size: 5509 AhdI GACnn_n'nnGTC Cuts at: 4238 4238 Size: 5509 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5509 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5509 AlwNI CAG_nnn'CTG Cuts at: 2333 3252 4824 2333 Size: 919 1572 3018 Fragments arranged by size: 3018 1572 919 ApaLI G'TGCA_C Cuts at: 3566 4722 3566 Size: 1156 4353 ApoI r'AATT_y Cuts at: 1493 2818 3044 3196 5305 1493 Size: 1325 226 152 2109 1697 Fragments arranged by size: 2109 1697 1325 226 152 AseI AT'TA_AT Cuts at: 160 1032 4063 160 Size: 872 3031 1606 Fragments arranged by size: 3031 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 AvaI C'yCGr_G Cuts at: 1870 1943 2070 2834 1870 Size: 73 127 764 4545 Fragments arranged by size: 4545 764 127 73 BamHI G'GATC_C Cuts at: 2839 3206 2839 Size: 367 5142 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2350 2384 2636 2830 4285 Size: 325 217 627 563 34 252 194 1455 Cuts at: 4285 618 Size: 1842 Fragments arranged by size: 1842 1455 627 563 325 252 217 194 34 BanII G_rGCy'C Cuts at: 1391 2012 2135 2507 2828 1391 Size: 621 123 372 321 4072 Fragments arranged by size: 4072 621 372 321 123 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2246 2310 928 Size: 971 347 64 4127 Fragments arranged by size: 4127 971 347 64 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2051 2085 3700 3734 1903 Size: 34 114 34 1615 34 3678 Fragments arranged by size: 3678 1615 114 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3401 4611 3401 Size: 1210 4299 BclI T'GATC_A Cuts at: 1548 1548 Size: 5509 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4120 136 Size: 107 122 71 631 605 2448 1525 Fragments arranged by size: 2448 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2540 5504 2540 Size: 2964 2545 BlpI GC'TnA_GC Cuts at: 2011 2926 2011 Size: 915 4594 Bme1580I G_kGCm'C Cuts at: 1790 3570 4726 1790 Size: 1780 1156 2573 Fragments arranged by size: 2573 1780 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5509 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2021 2383 3240 Size: 416 53 83 399 24 604 362 857 Cuts at: 3240 4198 442 Size: 958 1753 Fragments arranged by size: 1753 958 857 604 416 399 362 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5509 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 5509 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2189 2760 4169 1239 Size: 950 571 1409 2579 Fragments arranged by size: 2579 1409 950 571 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2503 3634 4499 4797 2503 Size: 1131 865 298 3215 Fragments arranged by size: 3215 1131 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2247 4172 882 Size: 617 748 1925 2219 Fragments arranged by size: 2219 1925 748 617 BsaAI yAC'GTr Cuts at: 493 1204 3300 493 Size: 711 2096 2702 Fragments arranged by size: 2702 2096 711 BsaBI GATnn'nnATC Cuts at: 5503 5503 Size: 5509 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3316 3698 4333 275 Size: 53 83 186 936 1783 382 635 1451 Fragments arranged by size: 1783 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3942 4340 4614 4761 5172 3942 Size: 398 274 147 411 4279 Fragments arranged by size: 4279 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5284 Fragments arranged by size: 5284 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5509 BseYI C'CCAG_C Cuts at: 4712 5208 4712 Size: 496 5013 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4936 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2959 3720 4748 1540 Size: 275 186 958 761 1028 2301 Fragments arranged by size: 2301 1028 958 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2135 2507 3570 3655 4726 2135 Size: 372 1063 85 1071 2918 Fragments arranged by size: 2918 1071 1063 372 85 BsmI GAATG_Cn' Cuts at: 3020 3113 3020 Size: 93 5416 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2012 2135 2507 2828 3570 3655 4726 Size: 399 222 123 372 321 742 85 1071 Cuts at: 4726 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 372 321 222 123 85 BspEI T'CCGG_A Cuts at: 5172 5172 Size: 5509 BspHI T'CATG_A Cuts at: 3398 5128 3398 Size: 1730 3779 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2864 844 Size: 2020 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2205 3396 1394 Size: 429 382 1191 3507 Fragments arranged by size: 3507 1191 429 382 BsrDI GCAATG_nn' Cuts at: 65 1248 4004 4178 65 Size: 1183 2756 174 1396 Fragments arranged by size: 2756 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4153 4340 1700 Size: 110 2343 187 2869 Fragments arranged by size: 2869 2343 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5509 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5509 BssSI C'ACGA_G Cuts at: 3569 4581 3569 Size: 1012 4497 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5509 BstBI TT'CG_AA Cuts at: 4313 4313 Size: 5509 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5509 BstXI CCAn_nnnn'nTGG Cuts at: 2125 2125 Size: 5509 BstYI r'GATC_y Cuts at: 1972 2197 2540 2597 2839 3206 3591 3608 5060 Size: 225 343 57 242 367 385 17 1452 Cuts at: 5060 5146 5158 5504 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 385 367 346 343 242 225 86 57 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4018 Fragments arranged by size: 4018 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4770 Fragments arranged by size: 4770 683 56 BtsI GCAGTG_nn' Cuts at: 2254 3025 3819 3839 2254 Size: 771 794 20 3924 Fragments arranged by size: 3924 794 771 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5509 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5474 DraI TTT'AAA Cuts at: 2813 2813 Size: 5509 DraIII CAC_nnn'GTG Cuts at: 2096 2096 Size: 5509 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4516 809 Size: 507 3200 1802 Fragments arranged by size: 3200 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2493 2956 3845 Size: 54 1425 50 275 18 663 463 889 Cuts at: 3845 5474 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 663 463 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2956 1537 Size: 275 1144 4090 Fragments arranged by size: 4090 1144 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3439 1353 Size: 2086 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2037 2093 4096 4470 4616 387 Size: 1064 138 448 56 2003 374 146 1280 Fragments arranged by size: 2003 1280 1064 448 374 146 138 56 EcoICRI GAG'CTC Cuts at: 2133 2505 2133 Size: 372 5137 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2189 2322 2529 2760 3572 4169 4956 Size: 393 557 133 207 231 812 597 787 Cuts at: 4956 5224 1239 Size: 268 1524 Fragments arranged by size: 1524 812 787 597 557 393 268 231 207 133 EcoO109I rG'GnC_Cy Cuts at: 2524 2524 Size: 5509 EcoRI G'AATT_C Cuts at: 2818 3196 2818 Size: 378 5131 FspI TGC'GCA Cuts at: 2111 2558 4015 2111 Size: 447 1457 3605 Fragments arranged by size: 3605 1457 447 HaeII r_GCGC'y Cuts at: 1678 4656 1678 Size: 2978 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2189 2221 2297 2329 2501 Size: 32 135 32 617 32 76 32 172 Cuts at: 2501 2533 2589 2612 2621 2644 4150 4182 4224 Size: 32 56 23 9 23 1506 32 42 Cuts at: 4224 4256 1373 Size: 32 2626 Fragments arranged by size: 2626 1506 617 172 135 76 56 42 32 32 32 32 32 32 32 23 23 9 HincII GTy'rAC Cuts at: 669 1657 1938 2853 3099 669 Size: 988 281 915 246 3079 Fragments arranged by size: 3079 988 915 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5509 HpaI GTT'AAC Cuts at: 3099 3099 Size: 5509 KpnI G_GTAC'C Cuts at: 2388 2834 2388 Size: 446 5063 MfeI C'AATT_G Cuts at: 3108 3108 Size: 5509 MluI A'CGCG_T Cuts at: 5385 5385 Size: 5509 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2532 4623 4807 1305 Size: 1227 2091 184 2007 Fragments arranged by size: 2091 2007 1227 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2495 10 Size: 54 1425 343 663 3024 Fragments arranged by size: 3024 1425 663 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2123 3467 3826 3985 518 Size: 648 957 1344 359 159 2042 Fragments arranged by size: 2042 1344 957 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2281 2925 3602 4750 4995 1606 Size: 675 644 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 675 644 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4018 Fragments arranged by size: 4018 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5509 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5509 NotI GC'GGCC_GC Cuts at: 2956 2956 Size: 5509 NspI r_CATG'y Cuts at: 1132 1229 2186 2867 4412 1132 Size: 97 957 681 1545 2229 Fragments arranged by size: 2229 1545 957 681 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5509 PciI A'CATG_T Cuts at: 4408 4408 Size: 5509 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5509 PmeI GTTT'AAAC Cuts at: 2813 2813 Size: 5509 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3578 3610 5091 5123 3578 Size: 32 1481 32 3964 Fragments arranged by size: 3964 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2524 2524 Size: 5509 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5509 PsiI TTA'TAA Cuts at: 3079 3079 Size: 5509 PspXI vC'TCGA_Gb Cuts at: 2070 2070 Size: 5509 PstI C_TGCA'G Cuts at: 830 2619 2861 830 Size: 1789 242 3478 Fragments arranged by size: 3478 1789 242 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5509 PvuII CAG'CTG Cuts at: 2281 2281 Size: 5509 SacI G_AGCT'C Cuts at: 2135 2507 2135 Size: 372 5137 SalI G'TCGA_C Cuts at: 1936 2851 1936 Size: 915 4594 SanDI GG'GwC_CC Cuts at: 2524 2524 Size: 5509 SbfI CC_TGCA'GG Cuts at: 2619 2861 2619 Size: 242 5267 ScaI AGT'ACT Cuts at: 3757 5469 3757 Size: 1712 3797 SfcI C'TryA_G Cuts at: 826 1045 2615 2857 3992 4673 4864 826 Size: 219 1570 242 1135 681 191 1471 Fragments arranged by size: 1570 1471 1135 681 242 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5509 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5509 SmaI CCC'GGG Cuts at: 1872 2836 1872 Size: 964 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2070 2518 3613 4514 4776 820 Size: 197 926 127 448 1095 901 262 1553 Fragments arranged by size: 1553 1095 926 901 448 262 197 127 SnaBI TAC'GTA Cuts at: 493 3300 493 Size: 2807 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5509 SphI G_CATG'C Cuts at: 1132 2867 1132 Size: 1735 3774 SspI AAT'ATT Cuts at: 5 52 3433 5 Size: 47 3381 2081 Fragments arranged by size: 3381 2081 47 StuI AGG'CCT Cuts at: 1517 2148 1517 Size: 631 4878 StyI C'CwwG_G Cuts at: 513 1065 2004 2417 2457 2796 2948 513 Size: 552 939 413 40 339 152 3074 Fragments arranged by size: 3074 939 552 413 339 152 40 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3536 3553 3706 3891 4336 325 Size: 1255 1956 17 153 185 445 1498 Fragments arranged by size: 1956 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3755 5467 96 Size: 274 80 33 51 1443 1778 1712 138 Fragments arranged by size: 1778 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5469 5501 5469 Size: 32 5477 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5509 XbaI T'CTAG_A Cuts at: 2845 2845 Size: 5509 XhoI C'TCGA_G Cuts at: 1943 2070 1943 Size: 127 5382 XmaI C'CCGG_G Cuts at: 1870 2834 1870 Size: 964 4545 XmnI GAAnn'nnTTC Cuts at: 3638 3638 Size: 5509 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3317 276 Size: 53 83 186 936 1783 2468 Fragments arranged by size: 2468 1783 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI RsrII SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI