################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AccII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BamHI BbrPI BbvCI BciVI BfrI BglII BlnI BlpI Bpu1102I BsaAI BsiWI Bsp1407I BspDI BspMI BsrGI BssHII BssSI BstAPI BstBI BstEII BstPI BstUI CelII ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV EcoT22I HindIII HpaI KpnI KspI MluI MseI MvnI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AflIII 1280 ACATGT A^C[GA][CT]GT AvaI 1504 CCCGAG C^[CT]CG[GA]G AviII 167 TGCGCA TGC^GCA BalI 1013 TGGCCA TGG^CCA BanI 30 GGCGCC G^G[CT][GA]CC BbeI 30 GGCGCC GGCGC^C BbsI 682 GAAGAC GAAGAC(2/6) BcgI 524 GCATCGCCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 131 TGATCA T^GATCA BfaI 857 CTAG C^TAG BglI 388 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BpuAI 682 GAAGAC GAAGAC(2/6) BsaBI 132 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 946 GTGCAG GTGCAG(16/14) BsiEI 258 CGACCG CG[GA][CT]^CG BsmI 1571 GAATGC GAATGC(1/-1) BsoBI 1504 CCCGAG C^[CT]CG[GA]G BspHI 884 TCATGA T^CATGA BspLU11I 1280 ACATGT A^CATGT BsrBI 121 GAGCGG CCGCTC(-3/-3) BsrDI 1347 CATTGC GCAATG(2/0) Bst1107I 1428 GTATAC GTA^TAC BstZ17I 1428 GTATAC GTA^TAC EcoNI 1112 CCTCCATGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT14I 1076 CCAAGG C^C[AT][AT]GG FbaI 131 TGATCA T^GATCA FseI 512 GGCCGGCC GGCCGG^CC FspI 167 TGCGCA TGC^GCA HincII 328 GTCAAC GT[CT]^[GA]AC HindII 328 GTCAAC GT[CT]^[GA]AC KasI 30 GGCGCC G^GCGCC MaeI 857 CTAG C^TAG MamI 132 GATCAATATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 163 CAATTG C^AATTG MluNI 1013 TGGCCA TGG^CCA MscI 1013 TGGCCA TGG^CCA MslI 1290 CATGACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 163 CAATTG C^AATTG NarI 30 GGCGCC GG^CGCC NspI 1280 ACATGT [GA]CATG^[CT] PflMI 967 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1337 GGGTCCT [GA]G^G[AT]CC[CT] PstI 1084 CTGCAG CTGCA^G PvuII 373 CAGCTG CAG^CTG RcaI 884 TCATGA T^CATGA SfiI 387 GGCCCTGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 30 GGCGCC GGC^GCC StuI 1109 AGGCCT AGG^CCT StyI 1076 CCAAGG C^C[AT][AT]GG Tsp45I 1438 GTCAC ^GT[GC]AC Tsp509I 164 AATT ^AATT Van91I 967 CCAGCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 1180 GTCTAC GT^[AC][GT]AC 1428 GTATAC AccIII 186 TCCGGA T^CCGGA 875 TCCGGA AcyI 30 GGCGCC G[GA]^CG[CT]C 280 GACGCC AlwI 360 GATCC GGATC(4/5) 1089 GATCC AlwNI 1087 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG 1190 CAGAGGCTG AocI 237 CCTGAGG CC^T[ACGT]AGG 1464 CCTGAGG AspHI 504 GAGCTC G[AT]GC[AT]^C 829 GAGCTC BsaHI 30 GGCGCC G[GA]^CG[CT]C 280 GACGCC BsaWI 186 TCCGGA [AT]^CCGG[AT] 875 TCCGGA BseAI 186 TCCGGA T^CCGGA 875 TCCGGA BsiHKAI 504 GAGCTC G[AT]GC[AT]^C 829 GAGCTC BspEI 186 TCCGGA T^CCGGA 875 TCCGGA BstXI 1004 CCATGTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1289 CCATGACTGTGG BstYI 359 AGATCC [GA]^GATC[CT] 1088 AGATCC Bsu36I 237 CCTGAGG CC^T[ACGT]AGG 1464 CCTGAGG DraII 386 AGGCCCT [GA]G^G[ACGT]CC[CT] 1337 GGGTCCT DsaI 45 CCGTGG C^C[GA][CT]GG 1262 CCACGG Eco81I 237 CCTGAGG CC^T[ACGT]AGG 1464 CCTGAGG EcoO109I 386 AGGCCCT [GA]G^G[ACGT]CC[CT] 1337 GGGTCCT HaeII 30 GGCGCC [GA]GCGC^[CT] 470 AGCGCC HgaI 280 GACGC GACGC(5/10) 801 GACGC Hin1I 30 GGCGCC G[GA]^CG[CT]C 280 GACGCC MaeII 332 ACGT A^CGT 1436 ACGT MflI 359 AGATCC [GA]^GATC[CT] 1088 AGATCC MroI 186 TCCGGA T^CCGGA 875 TCCGGA MspA1I 373 CAGCTG C[AC]G^C[GT]G 448 CCGCTG SacI 504 GAGCTC GAGCT^C 829 GAGCTC ScaI 248 AGTACT AGT^ACT 754 AGTACT SfcI 111 CTGTAG C^T[GA][CT]AG 1084 CTGCAG TfiI 688 GATTC G^A[AT]TC 1207 GATTC XcmI 1115 CCATGAGGGAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1434 CCACGTCACTTGTGG XhoII 359 AGATCC [GA]^GATC[CT] 1088 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### AciI AfaI AluI AvaII BanII BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmBI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MnlI MspI MvaI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TaqI TseI TspRI TthHB8I