(Circular) (Six-base) MAPSORT of: pFN21AE3980.seq Check: 2297 from: 1 to: 6292 pFN21AE3980 6292 bp With 182 enzymes: SgfI * March 28, 2012 14:36 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4102 278 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3186 3434 3635 1937 Size: 1249 248 201 4594 Fragments arranged by size: 4594 1249 248 201 Acc65I G'GTAC_C Cuts at: 3613 3613 Size: 6292 AclI AA'CG_TT Cuts at: 1563 4419 4792 1563 Size: 2856 373 3063 Fragments arranged by size: 3063 2856 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4355 5739 6007 1632 Size: 2723 1384 268 1917 Fragments arranged by size: 2723 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6095 AflIII A'CryG_T Cuts at: 1203 3285 5191 6168 1203 Size: 2082 1906 977 1327 Fragments arranged by size: 2082 1906 1327 977 AgeI A'CCGG_T Cuts at: 5123 5123 Size: 6292 AhdI GACnn_n'nnGTC Cuts at: 5021 5021 Size: 6292 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6292 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2091 3125 1184 Size: 907 1034 4351 Fragments arranged by size: 4351 1034 907 AlwNI CAG_nnn'CTG Cuts at: 3097 3200 4035 5607 3097 Size: 103 835 1572 3782 Fragments arranged by size: 3782 1572 835 103 ApaLI G'TGCA_C Cuts at: 4349 5505 4349 Size: 1156 5136 ApoI r'AATT_y Cuts at: 1493 3601 3827 3979 6088 1493 Size: 2108 226 152 2109 1697 Fragments arranged by size: 2109 2108 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4846 160 Size: 872 3814 1606 Fragments arranged by size: 3814 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 AvaI C'yCGr_G Cuts at: 1870 1943 3509 3617 1870 Size: 73 1566 108 4545 Fragments arranged by size: 4545 1566 108 73 BamHI G'GATC_C Cuts at: 3622 3989 3622 Size: 367 5925 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2035 3613 5068 618 Size: 325 217 627 248 1578 1455 1842 Fragments arranged by size: 1842 1578 1455 627 325 248 217 BanII G_rGCy'C Cuts at: 1391 2513 2747 2774 2838 3323 3611 1391 Size: 1122 234 27 64 485 288 4072 Fragments arranged by size: 4072 1122 485 288 234 64 27 BbeI G_GCGC'C Cuts at: 2039 2039 Size: 6292 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2694 928 Size: 971 795 4526 Fragments arranged by size: 4526 971 795 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2518 2552 4483 4517 1903 Size: 34 581 34 1931 34 3678 Fragments arranged by size: 3678 1931 581 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4184 5394 4184 Size: 1210 5082 BclI T'GATC_A Cuts at: 1548 2136 1548 Size: 588 5704 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2399 4903 136 Size: 107 122 71 631 605 727 2504 1525 Fragments arranged by size: 2504 1525 727 631 605 122 107 71 BglII A'GATC_T Cuts at: 6287 6287 Size: 6292 BlpI GC'TnA_GC Cuts at: 3709 3709 Size: 6292 Bme1580I G_kGCm'C Cuts at: 1790 2343 2942 3369 4353 5509 1790 Size: 553 599 427 984 1156 2573 Fragments arranged by size: 2573 1156 984 599 553 427 BmgBI CAC'GTC Cuts at: 1318 3442 1318 Size: 2124 4168 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2590 2645 4023 Size: 416 53 83 399 24 1173 55 1378 Cuts at: 4023 4981 442 Size: 958 1753 Fragments arranged by size: 1753 1378 1173 958 416 399 83 55 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6292 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2378 2603 3160 4952 1239 Size: 1139 225 557 1792 2579 Fragments arranged by size: 2579 1792 1139 557 225 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4417 5282 5580 4417 Size: 865 298 5129 Fragments arranged by size: 5129 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2202 2579 3523 4955 882 Size: 617 703 377 944 1432 2219 Fragments arranged by size: 2219 1432 944 703 617 377 BsaAI yAC'GTr Cuts at: 493 1204 4083 493 Size: 711 2879 2702 Fragments arranged by size: 2879 2702 711 BsaBI GATnn'nnATC Cuts at: 2141 6286 2141 Size: 4145 2147 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2036 2286 4099 4481 Size: 53 83 186 936 503 250 1813 382 Cuts at: 4481 5116 275 Size: 635 1451 Fragments arranged by size: 1813 1451 936 635 503 382 250 186 83 53 BsaWI w'CCGG_w Cuts at: 2191 2880 4725 5123 5397 5544 5955 2191 Size: 689 1845 398 274 147 411 2528 Fragments arranged by size: 2528 1845 689 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2795 2825 1178 Size: 30 165 30 1392 30 4645 Fragments arranged by size: 4645 1392 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2375 2693 2737 2740 2997 3434 1186 Size: 1189 318 44 3 257 437 4044 Fragments arranged by size: 4044 1189 437 318 257 44 3 BseYI C'CCAG_C Cuts at: 2276 2380 2463 3130 3224 3338 3533 5495 5991 Size: 104 83 667 94 114 195 1962 496 Cuts at: 5991 2276 Size: 2577 Fragments arranged by size: 2577 1962 667 496 195 114 104 94 83 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2972 1163 Size: 573 1236 4483 Fragments arranged by size: 4483 1236 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2266 3742 4503 5531 1540 Size: 275 186 265 1476 761 1028 2301 Fragments arranged by size: 2301 1476 1028 761 275 265 186 BsiHKAI G_wGCw'C Cuts at: 2513 2838 4353 4438 5509 2513 Size: 325 1515 85 1071 3296 Fragments arranged by size: 3296 1515 1071 325 85 BsmI GAATG_Cn' Cuts at: 3582 3803 3896 3582 Size: 221 93 5978 Fragments arranged by size: 5978 221 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2545 2798 3382 3401 2545 Size: 253 584 19 5436 Fragments arranged by size: 5436 584 253 19 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2343 2513 2747 2774 2838 2942 3323 Size: 399 553 170 234 27 64 104 381 Cuts at: 3323 3369 3611 4353 4438 5509 1391 Size: 46 242 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 553 399 381 242 234 170 104 85 64 46 27 BspEI T'CCGG_A Cuts at: 2191 2880 5955 2191 Size: 689 3075 2528 Fragments arranged by size: 3075 2528 689 BspHI T'CATG_A Cuts at: 2889 4181 5911 2889 Size: 1292 1730 3270 Fragments arranged by size: 3270 1730 1292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3647 844 Size: 2803 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2128 4179 1394 Size: 429 305 2051 3507 Fragments arranged by size: 3507 2051 429 305 BsrDI GCAATG_nn' Cuts at: 65 1248 3351 4787 4961 65 Size: 1183 2103 1436 174 1396 Fragments arranged by size: 2103 1436 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2096 2217 2271 2329 2518 2869 4936 Size: 110 286 121 54 58 189 351 2067 Cuts at: 4936 5123 1700 Size: 187 2869 Fragments arranged by size: 2869 2067 351 286 189 187 121 110 58 54 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6292 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6292 BssSI C'ACGA_G Cuts at: 4352 5364 4352 Size: 1012 5280 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6292 BstBI TT'CG_AA Cuts at: 5096 5096 Size: 6292 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6292 BstXI CCAn_nnnn'nTGG Cuts at: 3016 3301 3016 Size: 285 6007 BstYI r'GATC_y Cuts at: 1972 2364 3093 3622 3989 4374 4391 5843 5929 Size: 392 729 529 367 385 17 1452 86 Cuts at: 5929 5941 6287 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 729 529 392 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 3435 3435 Size: 6292 Bsu36I CC'TnA_GG Cuts at: 2243 3470 2243 Size: 1227 5065 BtgI C'CryG_G Cuts at: 513 1065 2050 3267 513 Size: 552 985 1217 3538 Fragments arranged by size: 3538 1217 985 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2515 3319 487 Size: 683 56 1289 804 3460 Fragments arranged by size: 3460 1289 804 683 56 BtsI GCAGTG_nn' Cuts at: 3258 3808 4602 4622 3258 Size: 550 794 20 4928 Fragments arranged by size: 4928 794 550 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6292 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3428 3463 529 Size: 35 2864 35 3358 Fragments arranged by size: 3358 2864 35 35 DraI TTT'AAA Cuts at: 3596 3596 Size: 6292 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5299 809 Size: 507 3983 1802 Fragments arranged by size: 3983 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2215 2520 2989 Size: 54 1425 50 275 18 385 305 469 Cuts at: 2989 3018 3739 4628 6257 8 Size: 29 721 889 1629 43 Fragments arranged by size: 1629 1425 889 721 469 385 305 275 54 50 43 29 18 EagI C'GGCC_G Cuts at: 1537 1812 3739 1537 Size: 275 1927 4090 Fragments arranged by size: 4090 1927 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2670 3158 3176 4222 1353 Size: 1317 488 18 1046 3423 Fragments arranged by size: 3423 1317 1046 488 18 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2842 3026 4879 5253 5399 387 Size: 1064 138 1253 184 1853 374 146 1280 Fragments arranged by size: 1853 1280 1253 1064 374 184 146 138 EcoICRI GAG'CTC Cuts at: 2511 2836 2511 Size: 325 5967 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2378 2603 3160 4355 4952 5739 6007 Size: 393 746 225 557 1195 597 787 268 Cuts at: 6007 1239 Size: 1524 Fragments arranged by size: 1524 1195 787 746 597 557 393 268 225 EcoNI CCTnn'n_nnAGG Cuts at: 3121 3121 Size: 6292 EcoO109I rG'GnC_Cy Cuts at: 2392 3343 2392 Size: 951 5341 EcoRI G'AATT_C Cuts at: 3601 3979 3601 Size: 378 5914 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2890 3520 2890 Size: 630 5662 FseI GG_CCGG'CC Cuts at: 2522 2522 Size: 6292 FspI TGC'GCA Cuts at: 2174 4798 2174 Size: 2624 3668 HaeII r_GCGC'y Cuts at: 1678 2039 2479 5439 1678 Size: 361 440 2960 2531 Fragments arranged by size: 2960 2531 440 361 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4933 4965 5007 5039 1373 Size: 32 135 32 3361 32 42 32 2626 Fragments arranged by size: 3361 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2335 3636 3882 669 Size: 988 281 397 1301 246 3079 Fragments arranged by size: 3079 1301 988 397 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6292 HpaI GTT'AAC Cuts at: 3882 3882 Size: 6292 KasI G'GCGC_C Cuts at: 2035 2035 Size: 6292 KpnI G_GTAC'C Cuts at: 3617 3617 Size: 6292 MfeI C'AATT_G Cuts at: 2168 3891 2168 Size: 1723 4569 MluI A'CGCG_T Cuts at: 6168 6168 Size: 6292 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3405 5406 5590 1305 Size: 2100 2001 184 2007 Fragments arranged by size: 2100 2007 2001 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3020 10 Size: 54 1425 343 1188 3282 Fragments arranged by size: 3282 1425 1188 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3299 4250 4609 4768 518 Size: 648 2133 951 359 159 2042 Fragments arranged by size: 2133 2042 951 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2380 2455 3708 4385 5533 5778 1606 Size: 774 75 1253 677 1148 245 2120 Fragments arranged by size: 2120 1253 1148 774 677 245 75 NaeI GCC'GGC Cuts at: 2098 2219 2520 2871 2098 Size: 121 301 351 5519 Fragments arranged by size: 5519 351 301 121 NarI GG'CG_CC Cuts at: 2036 2036 Size: 6292 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5740 NdeI CA'TA_TG Cuts at: 387 387 Size: 6292 NgoMIV G'CCGG_C Cuts at: 2096 2217 2518 2869 2096 Size: 121 301 351 5519 Fragments arranged by size: 5519 351 301 121 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6292 NotI GC'GGCC_GC Cuts at: 3739 3739 Size: 6292 NspI r_CATG'y Cuts at: 1132 1229 3289 3650 5195 1132 Size: 97 2060 361 1545 2229 Fragments arranged by size: 2229 2060 1545 361 97 PasI CC'CwG_GG Cuts at: 1348 2723 2935 1348 Size: 1375 212 4705 Fragments arranged by size: 4705 1375 212 PciI A'CATG_T Cuts at: 3285 5191 3285 Size: 1906 4386 PflMI CCAn_nnn'nTGG Cuts at: 1108 2978 1108 Size: 1870 4422 PfoI T'CCnGG_A Cuts at: 2088 2355 2406 3231 2088 Size: 267 51 825 5149 Fragments arranged by size: 5149 825 267 51 PmeI GTTT'AAAC Cuts at: 3596 3596 Size: 6292 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4361 4393 5874 5906 4361 Size: 32 1481 32 4747 Fragments arranged by size: 4747 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3343 3343 Size: 6292 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6292 PsiI TTA'TAA Cuts at: 3862 3862 Size: 6292 PstI C_TGCA'G Cuts at: 830 3093 3644 830 Size: 2263 551 3478 Fragments arranged by size: 3478 2263 551 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6292 PvuII CAG'CTG Cuts at: 2380 2380 Size: 6292 SacI G_AGCT'C Cuts at: 2513 2838 2513 Size: 325 5967 SalI G'TCGA_C Cuts at: 1936 3634 1936 Size: 1698 4594 SbfI CC_TGCA'GG Cuts at: 3644 3644 Size: 6292 ScaI AGT'ACT Cuts at: 2255 2761 4540 6252 2255 Size: 506 1779 1712 2295 Fragments arranged by size: 2295 1779 1712 506 SfcI C'TryA_G Cuts at: 826 1045 2116 3089 3640 4775 5456 5647 826 Size: 219 1071 973 551 1135 681 191 1471 Fragments arranged by size: 1471 1135 1071 973 681 551 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2399 2399 Size: 6292 SfoI GGC'GCC Cuts at: 2037 2037 Size: 6292 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6292 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6292 SmaI CCC'GGG Cuts at: 1872 3619 1872 Size: 1747 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4396 5297 5559 820 Size: 197 926 2453 901 262 1553 Fragments arranged by size: 2453 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4083 493 Size: 3590 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6292 SphI G_CATG'C Cuts at: 1132 3650 1132 Size: 2518 3774 SspI AAT'ATT Cuts at: 5 52 4216 5 Size: 47 4164 2081 Fragments arranged by size: 4164 2081 47 StuI AGG'CCT Cuts at: 1517 3116 1517 Size: 1599 4693 StyI C'CwwG_G Cuts at: 513 1065 3081 3731 513 Size: 552 2016 650 3074 Fragments arranged by size: 3074 2016 650 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4319 4336 4489 4674 5119 325 Size: 1255 2739 17 153 185 445 1498 Fragments arranged by size: 2739 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2253 2759 4538 Size: 274 80 33 51 1443 276 506 1779 Cuts at: 4538 6250 96 Size: 1712 138 Fragments arranged by size: 1779 1712 1443 506 276 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2795 2827 3300 3332 6252 6284 2795 Size: 32 473 32 2920 32 2803 Fragments arranged by size: 2920 2803 473 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6292 XbaI T'CTAG_A Cuts at: 3628 3628 Size: 6292 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3127 3446 3127 Size: 319 5973 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6292 XmaI C'CCGG_G Cuts at: 1870 3617 1870 Size: 1747 4545 XmnI GAAnn'nnTTC Cuts at: 4421 4421 Size: 6292 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4100 276 Size: 53 83 186 936 2566 2468 Fragments arranged by size: 2566 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FseI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI DraIII EcoRV FspAI NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SrfI SwaI