################################## # NO CUT ENZYMES ( 183 enzymes) # ################################## AatII Acc65I AccI AccII AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BcnI BfrI BglI BglII BlnI BlpI BmyI Bpu1102I BsaAI BsaBI BsaHI BsaI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1286I BspDI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BssHII Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DpnII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeII MamI MboI MfeI MflI MluI MluNI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NdeII NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI Sau3AI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TaqI Tth111I TthHB8I Van91I XbaI XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 36 enzymes) # ################################### AccIII 164 TCCGGA T^CCGGA AvaII 912 GGTCC G^G[AT]CC BsaJI 999 CCTTGG C^C[ACGT][ACGT]GG BsaWI 164 TCCGGA [AT]^CCGG[AT] BseAI 164 TCCGGA T^CCGGA BseRI 416 CTCCTC GAGGAG(10/8) BsmFI 913 GTCCC GGGAC(10/14) Bsp1407I 253 TGTACA T^GTACA BspEI 164 TCCGGA T^CCGGA BsrGI 253 TGTACA T^GTACA BssKI 604 CCTGG ^CC[ACGT]GG BssSI 1019 CACGAG CACGAG(-5/-1) BstNI 604 CCTGG CC^[AT]GG Cfr13I 912 GGTCC G^G[ACGT]CC DraI 93 TTTAAA TTT^AAA DraII 911 GGGTCCC [GA]G^G[ACGT]CC[CT] DraIII 602 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO109I 911 GGGTCCC [GA]G^G[ACGT]CC[CT] EcoRII 604 CCTGG ^CC[AT]GG EcoT14I 999 CCTTGG C^C[AT][AT]GG HaeIII 8 GGCC GG^CC HapII 165 CCGG C^CGG HpaII 165 CCGG C^CGG MroI 164 TCCGGA T^CCGGA MspI 165 CCGG C^CGG MvaI 604 CCTGG CC^[AT]GG NlaIII 382 CATG CATG^ PpuMI 911 GGGTCCC [GA]G^G[AT]CC[CT] PstI 527 CTGCAG CTGCA^G Sau96I 912 GGTCC G^G[ACGT]CC ScrFI 604 CCTGG CC^[ACGT]GG SexAI 603 ACCTGGT A^CC[AT]GGT SfcI 527 CTGCAG C^T[GA][CT]AG SmlI 359 CTCAAG C^T[CT][GA]AG SspBI 253 TGTACA T^GTACA StyI 999 CCTTGG C^C[AT][AT]GG ################################### # TWO CUT ENZYMES ( 13 enzymes) # ################################### BbsI 658 GAAGAC GAAGAC(2/6) 843 GTCTTC BpmI 791 CTCCAG CTGGAG(16/14) 929 CTGGAG BpuAI 658 GAAGAC GAAGAC(2/6) 843 GTCTTC BsgI 242 GTGCAG GTGCAG(16/14) 349 GTGCAG BsiYI 915 CCCACCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 954 CCCACTGGTGG BslI 915 CCCACCAGCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 954 CCCACTGGTGG BstF5I 1036 CATCC GGATG(2/0) 621 GGATG FokI 1036 CATCC GGATG(9/13) 621 GGATG MaeIII 337 GTCAC ^GT[ACGT]AC 772 GTGAC MslI 342 CACTAATGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1036 CATCCAAATG NlaIV 911 GGGTCC GG[ACGT]^[ACGT]CC 912 GGTCCC PvuII 794 CAGCTG CAG^CTG 887 CAGCTG Tsp45I 337 GTCAC ^GT[GC]AC 772 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 26 enzymes) # ######################################### AciI AfaI AluI BbvI BfaI BsmAI BsrI DdeI Fnu4HI HinfI HphI ItaI MaeI MboII MnlI MseI MspA1I MwoI PleI RsaI SfaNI TfiI Tru9I TseI Tsp509I TspRI