(Circular) (Six-base) MAPSORT of: pFN21AE5415.seq Check: 1388 from: 1 to: 5761 pFN21AE5415 5761 bp With 182 enzymes: SgfI * January 30, 2014 17:19 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3571 278 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3104 1937 Size: 1167 4594 Acc65I G'GTAC_C Cuts at: 3082 3082 Size: 5761 AclI AA'CG_TT Cuts at: 1563 3888 4261 1563 Size: 2325 373 3063 Fragments arranged by size: 3063 2325 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2241 2835 2874 3824 5208 5476 1632 Size: 609 594 39 950 1384 268 1917 Fragments arranged by size: 1917 1384 950 609 594 268 39 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5564 AflIII A'CryG_T Cuts at: 1203 4660 5637 1203 Size: 3457 977 1327 Fragments arranged by size: 3457 1327 977 AgeI A'CCGG_T Cuts at: 4592 4592 Size: 5761 AhdI GACnn_n'nnGTC Cuts at: 4490 4490 Size: 5761 AleI CACnn'nnGTG Cuts at: 1166 2351 1166 Size: 1185 4576 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3063 1184 Size: 1879 3882 AlwNI CAG_nnn'CTG Cuts at: 3504 5076 3504 Size: 1572 4189 ApaLI G'TGCA_C Cuts at: 3818 4974 3818 Size: 1156 4605 ApoI r'AATT_y Cuts at: 1493 3070 3296 3448 5557 1493 Size: 1577 226 152 2109 1697 Fragments arranged by size: 2109 1697 1577 226 152 AseI AT'TA_AT Cuts at: 160 1032 4315 160 Size: 872 3283 1606 Fragments arranged by size: 3283 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 AvaI C'yCGr_G Cuts at: 1870 1943 3086 1870 Size: 73 1143 4545 Fragments arranged by size: 4545 1143 73 BamHI G'GATC_C Cuts at: 3091 3458 3091 Size: 367 5394 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3082 4537 618 Size: 325 217 627 1295 1455 1842 Fragments arranged by size: 1842 1455 1295 627 325 217 BanII G_rGCy'C Cuts at: 1391 3080 1391 Size: 1689 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2670 2841 928 Size: 971 771 171 3848 Fragments arranged by size: 3848 971 771 171 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3952 3986 1903 Size: 34 2015 34 3678 Fragments arranged by size: 3678 2015 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3653 4863 3653 Size: 1210 4551 BclI T'GATC_A Cuts at: 1548 1548 Size: 5761 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4372 136 Size: 107 122 71 631 605 2700 1525 Fragments arranged by size: 2700 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5756 5756 Size: 5761 BlpI GC'TnA_GC Cuts at: 3178 3178 Size: 5761 Bme1580I G_kGCm'C Cuts at: 1790 3822 4978 1790 Size: 2032 1156 2573 Fragments arranged by size: 2573 2032 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5761 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2180 2723 3492 Size: 416 53 83 399 24 763 543 769 Cuts at: 3492 4450 442 Size: 958 1753 Fragments arranged by size: 1753 958 769 763 543 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5761 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2781 2955 4421 1239 Size: 1542 174 1466 2579 Fragments arranged by size: 2579 1542 1466 174 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2349 3886 4751 5049 2349 Size: 1537 865 298 3061 Fragments arranged by size: 3061 1537 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4424 882 Size: 617 2925 2219 Fragments arranged by size: 2925 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3552 493 Size: 711 2348 2702 Fragments arranged by size: 2702 2348 711 BsaBI GATnn'nnATC Cuts at: 5755 5755 Size: 5761 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3568 3950 4585 275 Size: 53 83 186 936 2035 382 635 1451 Fragments arranged by size: 2035 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2169 4194 4592 4866 5013 5424 2169 Size: 2025 398 274 147 411 2506 Fragments arranged by size: 2506 2025 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5536 Fragments arranged by size: 5536 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2412 1186 Size: 1226 4535 BseYI C'CCAG_C Cuts at: 4964 5460 4964 Size: 496 5265 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2268 2375 1163 Size: 573 532 107 4549 Fragments arranged by size: 4549 573 532 107 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3211 3972 5000 1540 Size: 275 186 1210 761 1028 2301 Fragments arranged by size: 2301 1210 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3822 3907 4978 3822 Size: 85 1071 4605 Fragments arranged by size: 4605 1071 85 BsmI GAATG_Cn' Cuts at: 3272 3365 3272 Size: 93 5668 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3080 3822 3907 4978 1391 Size: 399 1290 742 85 1071 2174 Fragments arranged by size: 2174 1290 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2169 5424 2169 Size: 3255 2506 BspHI T'CATG_A Cuts at: 3650 5380 3650 Size: 1730 4031 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3116 844 Size: 2272 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3648 1394 Size: 429 1825 3507 Fragments arranged by size: 3507 1825 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4256 4430 65 Size: 1183 3008 174 1396 Fragments arranged by size: 3008 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4405 4592 1700 Size: 110 2595 187 2869 Fragments arranged by size: 2869 2595 187 110 BsrGI T'GTAC_A Cuts at: 96 2258 96 Size: 2162 3599 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5761 BssSI C'ACGA_G Cuts at: 3024 3821 4833 3024 Size: 797 1012 3952 Fragments arranged by size: 3952 1012 797 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5761 BstBI TT'CG_AA Cuts at: 4565 4565 Size: 5761 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5761 BstYI r'GATC_y Cuts at: 1972 3091 3458 3843 3860 5312 5398 5410 5756 Size: 1119 367 385 17 1452 86 12 346 Cuts at: 5756 1972 Size: 1977 Fragments arranged by size: 1977 1452 1119 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5022 Fragments arranged by size: 5022 683 56 BtsI GCAGTG_nn' Cuts at: 2256 2446 2572 3277 4071 4091 2256 Size: 190 126 705 794 20 3926 Fragments arranged by size: 3926 794 705 190 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5761 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5726 DraI TTT'AAA Cuts at: 2100 3065 2100 Size: 965 4796 DraIII CAC_nnn'GTG Cuts at: 2612 2612 Size: 5761 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4768 809 Size: 507 3452 1802 Fragments arranged by size: 3452 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3208 4097 5726 Size: 54 1425 50 275 18 1378 889 1629 Cuts at: 5726 8 Size: 43 Fragments arranged by size: 1629 1425 1378 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3208 1537 Size: 275 1396 4090 Fragments arranged by size: 4090 1396 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3691 1353 Size: 2338 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2990 4348 4722 4868 387 Size: 1064 138 1401 1358 374 146 1280 Fragments arranged by size: 1401 1358 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2241 2781 2835 2874 2955 3824 4421 Size: 393 609 540 54 39 81 869 597 Cuts at: 4421 5208 5476 1239 Size: 787 268 1524 Fragments arranged by size: 1524 869 787 609 597 540 393 268 81 54 39 EcoO109I rG'GnC_Cy Cuts at: 2917 2917 Size: 5761 EcoRI G'AATT_C Cuts at: 3070 3448 3070 Size: 378 5383 FspI TGC'GCA Cuts at: 4267 4267 Size: 5761 HaeII r_GCGC'y Cuts at: 1678 4908 1678 Size: 3230 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2735 2767 4402 4434 4476 Size: 32 135 32 1163 32 1635 32 42 Cuts at: 4476 4508 1373 Size: 32 2626 Fragments arranged by size: 2626 1635 1163 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3105 3351 669 Size: 988 281 1167 246 3079 Fragments arranged by size: 3079 1167 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5761 HpaI GTT'AAC Cuts at: 3351 3351 Size: 5761 KpnI G_GTAC'C Cuts at: 3086 3086 Size: 5761 MfeI C'AATT_G Cuts at: 3360 3360 Size: 5761 MluI A'CGCG_T Cuts at: 5637 5637 Size: 5761 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2360 4875 5059 1305 Size: 1055 2515 184 2007 Fragments arranged by size: 2515 2007 1055 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3939 Fragments arranged by size: 3939 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2351 3045 3719 4078 4237 518 Size: 648 1185 694 674 359 159 2042 Fragments arranged by size: 2042 1185 694 674 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2801 2894 2927 3177 3854 5002 5247 1606 Size: 1195 93 33 250 677 1148 245 2120 Fragments arranged by size: 2120 1195 1148 677 250 245 93 33 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4270 Fragments arranged by size: 4270 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5761 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5761 NotI GC'GGCC_GC Cuts at: 3208 3208 Size: 5761 NspI r_CATG'y Cuts at: 1132 1229 3119 4664 1132 Size: 97 1890 1545 2229 Fragments arranged by size: 2229 1890 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5761 PciI A'CATG_T Cuts at: 4660 4660 Size: 5761 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5761 PmeI GTTT'AAAC Cuts at: 3065 3065 Size: 5761 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3830 3862 5343 5375 3830 Size: 32 1481 32 4216 Fragments arranged by size: 4216 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2917 2917 Size: 5761 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5761 PsiI TTA'TAA Cuts at: 3331 3331 Size: 5761 PstI C_TGCA'G Cuts at: 830 2536 3113 830 Size: 1706 577 3478 Fragments arranged by size: 3478 1706 577 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5761 PvuII CAG'CTG Cuts at: 2801 2894 2801 Size: 93 5668 SalI G'TCGA_C Cuts at: 1936 3103 1936 Size: 1167 4594 SanDI GG'GwC_CC Cuts at: 2917 2917 Size: 5761 SbfI CC_TGCA'GG Cuts at: 3113 3113 Size: 5761 ScaI AGT'ACT Cuts at: 4009 5721 4009 Size: 1712 4049 SexAI A'CCwGG_T Cuts at: 2608 2608 Size: 5761 SfcI C'TryA_G Cuts at: 826 1045 2532 3109 4244 4925 5116 826 Size: 219 1487 577 1135 681 191 1471 Fragments arranged by size: 1487 1471 1135 681 577 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5761 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5761 SmaI CCC'GGG Cuts at: 1872 3088 1872 Size: 1216 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2364 3865 4766 5028 820 Size: 197 926 421 1501 901 262 1553 Fragments arranged by size: 1553 1501 926 901 421 262 197 SnaBI TAC'GTA Cuts at: 493 3552 493 Size: 3059 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5761 SphI G_CATG'C Cuts at: 1132 3119 1132 Size: 1987 3774 SspI AAT'ATT Cuts at: 5 52 3685 5 Size: 47 3633 2081 Fragments arranged by size: 3633 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5761 StyI C'CwwG_G Cuts at: 513 1065 2004 3004 3200 513 Size: 552 939 1000 196 3074 Fragments arranged by size: 3074 1000 939 552 196 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3788 3805 3958 4143 4588 325 Size: 1255 2208 17 153 185 445 1498 Fragments arranged by size: 2208 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2188 2258 4007 Size: 274 80 33 51 1443 211 70 1749 Cuts at: 4007 5719 96 Size: 1712 138 Fragments arranged by size: 1749 1712 1443 274 211 138 80 70 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5721 5753 5721 Size: 32 5729 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5761 XbaI T'CTAG_A Cuts at: 3097 3097 Size: 5761 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5761 XmaI C'CCGG_G Cuts at: 1870 3086 1870 Size: 1216 4545 XmnI GAAnn'nnTTC Cuts at: 3890 3890 Size: 5761 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3569 276 Size: 53 83 186 936 2035 2468 Fragments arranged by size: 2468 2035 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SapI SfiI SfoI SrfI SwaI XcmI