(Linear) (Six-base) MAPSORT of: KIEE5415.seq  Check: 3590  from: 1  to: 1056



KIEE5415.orf

 With 182 enzymes: SgfI * 

                        January 29, 2014 11:59  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     830     869    1056 
   Size:        236     594      39     187

  Fragments arranged by size: 

                 594     236     187      39
 
AleI CACnn'nnGTG

Cuts at:      0     346    1056 
   Size:        346     710
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     665     836    1056 
   Size:        665     171     220

  Fragments arranged by size: 

                 665     220     171
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     175     718    1056 
   Size:        175     543     338

  Fragments arranged by size: 

                 543     338     175
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     776     950    1056 
   Size:        776     174     106

  Fragments arranged by size: 

                 776     174     106
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     344    1056 
   Size:        344     712
 
BsaWI w'CCGG_w

Cuts at:      0     164    1056 
   Size:        164     892
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     407    1056 
   Size:        407     649
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     263     370    1056 
   Size:        263     107     686

  Fragments arranged by size: 

                 686     263     107
 
BspEI T'CCGG_A

Cuts at:      0     164    1056 
   Size:        164     892
 
BsrGI T'GTAC_A

Cuts at:      0     253    1056 
   Size:        253     803
 
BssSI C'ACGA_G

Cuts at:      0    1019    1056 
   Size:       1019      37
 
BtsI GCAGTG_nn'

Cuts at:      0     251     441     567    1056 
   Size:        251     190     126     489

  Fragments arranged by size: 

                 489     251     190     126
 
DraI TTT'AAA

Cuts at:      0      95    1056 
   Size:         95     961
 
DraIII CAC_nnn'GTG

Cuts at:      0     607    1056 
   Size:        607     449
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     985    1056 
   Size:        985      71
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     236     776     830     869     950    1056 
   Size:        236     540      54      39      81     106

  Fragments arranged by size: 

                 540     236     106      81      54      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     912    1056 
   Size:        912     144
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     730     762    1056 
   Size:        730      32     294

  Fragments arranged by size: 

                 730     294      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     355    1056 
   Size:        355     701
 
MslI CAynn'nnrTG

Cuts at:      0     346    1040    1056 
   Size:        346     694      16

  Fragments arranged by size: 

                 694     346      16
 
MspA1I CmG'CkG

Cuts at:      0     796     889     922    1056 
   Size:        796      93      33     134

  Fragments arranged by size: 

                 796     134      93      33
 
PpuMI rG'GwC_Cy

Cuts at:      0     912    1056 
   Size:        912     144
 
PstI C_TGCA'G

Cuts at:      0     531    1056 
   Size:        531     525
 
PvuII CAG'CTG

Cuts at:      0     796     889    1056 
   Size:        796      93     167

  Fragments arranged by size: 

                 796     167      93
 
SanDI GG'GwC_CC

Cuts at:      0     912    1056 
   Size:        912     144
 
SexAI A'CCwGG_T

Cuts at:      0     603    1056 
   Size:        603     453
 
SfcI C'TryA_G

Cuts at:      0     527    1056 
   Size:        527     529
 
SmlI C'TyrA_G

Cuts at:      0     359    1056 
   Size:        359     697
 
StyI C'CwwG_G

Cuts at:      0     999    1056 
   Size:        999      57
 
TatI w'GTAC_w

Cuts at:      0     183     253    1056 
   Size:        183      70     803

  Fragments arranged by size: 

                 803     183      70
 

 Enzymes that do cut:

     AcuI     AleI     BbsI     BmrI     BpmI    BpuEI    BsaWI    BseRI
     BsgI    BspEI    BsrGI    BssSI     BtsI     DraI   DraIII     EciI
  Eco57MI EcoO109I    Hin4I     MmeI     MslI   MspA1I    PpuMI     PstI
    PvuII    SanDI    SexAI     SfcI     SmlI     StyI     TatI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI
    BanII     BbeI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmtI     BplI   Bpu10I     BsaI
    BsaAI    BsaBI    BsaHI    BsaXI    BseYI    BsiEI  BsiHKAI    BsiWI
     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI    BsrFI
   BssHII   BstAPI    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I
     BtgI    BtgZI     ClaI    CspCI     DrdI     EaeI     EagI     EarI
  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI    FspAI
    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI
     MscI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI
     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI
     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PvuI    RsrII     SacI    SacII     SalI     SapI     SbfI     ScaI
     SfiI     SfoI     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI    TaqII     TstI  Tth111I     XbaI
     XcmI     XhoI     XmaI     XmnI     ZraI