Restriction map for
pFN21AE4368
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# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AvaII AviII BbeI BbrPI
BbsI BbvCI BciVI BclI BglI
BglII BlpI Bpu1102I BpuAI BsaAI
BsaHI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspMI BsrBI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstZ17I CelII
CfoI ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeII MfeI
MluI MroI MunI MvnI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI Psp1406I
PvuI RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccI 610 GTCTAC GT^[AC][GT]AC
AcsI 277 GAATTT [GA]^AATT[CT]
AflII 907 CTTAAG C^TTAAG
AflIII 800 ACATGT A^C[GA][CT]GT
AlwNI 968 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 205 CCTCAGG CC^T[ACGT]AGG
ApoI 277 GAATTT [GA]^AATT[CT]
AvrII 852 CCTAGG C^CTAGG
BalI 950 TGGCCA TGG^CCA
BamHI 992 GGATCC G^GATCC
BanI 71 GGCACC G^G[CT][GA]CC
BanII 127 GAGCCC G[GA]GC[CT]^C
BcgI 22 CGATTGGGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 853 CTAG C^TAG
BfrI 907 CTTAAG C^TTAAG
BlnI 852 CCTAGG C^CTAGG
BsaBI 750 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 630 GGTCTC GGTCTC(1/5)
BseRI 203 CTCCTC GAGGAG(10/8)
BsgI 30 GTGCAG GTGCAG(16/14)
BspLU11I 800 ACATGT A^CATGT
BsrDI 771 CATTGC GCAATG(2/0)
BsrFI 374 ACCGGC [GA]^CCGG[CT]
BstXI 75 CCAACTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 205 CCTCAGG CC^T[ACGT]AGG
Cac8I 952 GCCAGC GC[ACGT]^[ACGT]GC
Cfr10I 374 ACCGGC [GA]^CCGG[CT]
DsaI 1089 CCATGG C^C[GA][CT]GG
EaeI 950 TGGCCA [CT]^GGCC[GA]
Eco81I 205 CCTCAGG CC^T[ACGT]AGG
FauI 1130 GCGGG CCCGC(4/6)
HphI 97 GGTGA GGTGA(8/7)
MaeI 853 CTAG C^TAG
MaeIII 1022 GTGAC ^GT[ACGT]AC
MamI 750 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 950 TGGCCA TGG^CCA
MscI 950 TGGCCA TGG^CCA
MspA1I 1045 CAGCTG C[AC]G^C[GT]G
NcoI 1089 CCATGG C^CATGG
PleI 39 GACTC GAGTC(4/5)
PstI 1137 CTGCAG CTGCA^G
PvuII 1045 CAGCTG CAG^CTG
SfcI 1137 CTGCAG C^T[GA][CT]AG
StuI 855 AGGCCT AGG^CCT
TfiI 866 GAATC G^A[AT]TC
Tsp45I 1022 GTGAC ^GT[GC]AC
Tsp509I 278 AATT ^AATT
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AciI 914 CCGC CCGC(-3/-1)
1130 GCGG
AfaI 86 GTAC GT^AC
328 GTAC
BcnI 396 CCGGG CC^[GC]GG
989 CCCGG
BmyI 70 GGGCAC G[AGT]GC[ACT]^C
127 GAGCCC
BsmFI 797 GGGAC GGGAC(10/14)
234 GTCCC
Bsp1286I 70 GGGCAC G[AGT]GC[ACT]^C
127 GAGCCC
BspHI 321 TCATGA T^CATGA
1121 TCATGA
BstF5I 81 GGATG GGATG(2/0)
1149 GGATG
BstYI 488 GGATCT [GA]^GATC[CT]
992 GGATCC
FokI 81 GGATG GGATG(9/13)
1149 GGATG
HinfI 39 GACTC G^A[ACGT]TC
866 GAATC
MflI 488 GGATCT [GA]^GATC[CT]
992 GGATCC
MseI 407 TTAA T^TAA
908 TTAA
MslI 756 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1084 CACTTCCATG
NciI 396 CCGGG CC^[GC]GG
989 CCCGG
NspI 230 GCATGT [GA]CATG^[CT]
800 ACATGT
RcaI 321 TCATGA T^CATGA
1121 TCATGA
RsaI 86 GTAC GT^AC
328 GTAC
SmlI 343 CTCAAG C^T[CT][GA]AG
907 CTTAAG
Tru9I 407 TTAA T^TAA
908 TTAA
XhoII 488 GGATCT [GA]^GATC[CT]
992 GGATCC
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# 3 OR MORE CUT ENZYMES ( 40 enzymes) #
#########################################
AluI AlwI BbvI BpmI BsaJI
BsiYI BslI BsmAI BsrI BssKI
BstNI Cfr13I DdeI DpnII EarI
EcoRII EcoT14I Fnu4HI HaeIII HapII
HpaII ItaI Ksp632I MboI MboII
MnlI MspI MvaI MwoI NdeII
NlaIII NlaIV SapI Sau3AI Sau96I
ScrFI SfaNI StyI TseI TspRI