(Circular) (Six-base) MAPSORT of: pFN21AE4368.seq Check: 390 from: 1 to: 5863 pFN21AE4368 5863 bp With 182 enzymes: SgfI * March 28, 2012 13:32 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2616 3206 1937 Size: 679 590 4594 Fragments arranged by size: 4594 679 590 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3926 5310 5578 1632 Size: 2294 1384 268 1917 Fragments arranged by size: 2294 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 2912 820 Size: 197 1895 3771 Fragments arranged by size: 3771 1895 197 AflIII A'CryG_T Cuts at: 1203 2805 4762 5739 1203 Size: 1602 1957 977 1327 Fragments arranged by size: 1957 1602 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 4592 4592 Size: 5863 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2682 2714 2997 3029 3069 3101 2682 Size: 32 283 32 40 32 5444 Fragments arranged by size: 5444 283 40 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 2978 3606 5178 2978 Size: 628 1572 3663 Fragments arranged by size: 3663 1572 628 ApaLI G'TGCA_C Cuts at: 3920 5076 3920 Size: 1156 4707 ApoI r'AATT_y Cuts at: 1493 2282 3172 3398 3550 5659 1493 Size: 789 890 226 152 2109 1697 Fragments arranged by size: 2109 1697 890 789 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 3188 1870 Size: 73 1245 4545 Fragments arranged by size: 4545 1245 73 AvrII C'CTAG_G Cuts at: 2857 2857 Size: 5863 BamHI G'GATC_C Cuts at: 2997 3193 3560 2997 Size: 196 367 5300 Fragments arranged by size: 5300 367 196 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2076 3184 4639 618 Size: 325 217 627 289 1108 1455 1842 Fragments arranged by size: 1842 1455 1108 627 325 289 217 BanII G_rGCy'C Cuts at: 1391 2136 3182 1391 Size: 745 1046 4072 Fragments arranged by size: 4072 1046 745 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4892 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2016 2050 4054 4088 1903 Size: 34 79 34 2004 34 3678 Fragments arranged by size: 3678 2004 79 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3755 4965 3755 Size: 1210 4653 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4474 136 Size: 107 122 71 631 605 2802 1525 Fragments arranged by size: 2802 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 3280 3280 Size: 5863 Bme1580I G_kGCm'C Cuts at: 1790 2079 3924 5080 1790 Size: 289 1845 1156 2573 Fragments arranged by size: 2573 1845 1156 289 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2791 3594 4552 Size: 416 53 83 399 24 1374 803 958 Cuts at: 4552 442 Size: 1753 Fragments arranged by size: 1753 1374 958 803 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2597 2653 2597 Size: 56 5807 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2031 3095 3096 4523 1239 Size: 792 1064 1 1427 2579 Fragments arranged by size: 2579 1427 1064 792 1 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2333 3988 4853 5151 2333 Size: 1655 865 298 3045 Fragments arranged by size: 3045 1655 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2641 4526 882 Size: 617 1142 1885 2219 Fragments arranged by size: 2219 1885 1142 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 2759 5857 2759 Size: 3098 2765 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3670 4052 4687 275 Size: 53 83 186 936 2137 382 635 1451 Fragments arranged by size: 2137 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4296 4694 4968 5115 5526 4296 Size: 398 274 147 411 4633 Fragments arranged by size: 4633 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3069 3099 1178 Size: 30 165 30 1666 30 3942 Fragments arranged by size: 3942 1666 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2199 1186 Size: 1013 4850 BseYI C'CCAG_C Cuts at: 2770 5066 5562 2770 Size: 2296 496 3071 Fragments arranged by size: 3071 2296 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2056 1163 Size: 573 320 4970 Fragments arranged by size: 4970 573 320 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3924 4009 5080 3924 Size: 85 1071 4707 Fragments arranged by size: 4707 1071 85 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2079 2136 3182 3924 4009 5080 1391 Size: 399 289 57 1046 742 85 1071 2174 Fragments arranged by size: 2174 1071 1046 742 399 289 85 57 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 2326 3126 3752 5482 2326 Size: 800 626 1730 2707 Fragments arranged by size: 2707 1730 800 626 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3218 844 Size: 2374 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2775 4358 4532 65 Size: 1183 1527 1583 174 1396 Fragments arranged by size: 1583 1527 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2379 4507 4694 1700 Size: 110 569 2128 187 2869 Fragments arranged by size: 2869 2128 569 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5863 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5863 BstXI CCAn_nnnn'nTGG Cuts at: 2087 2087 Size: 5863 BstYI r'GATC_y Cuts at: 1972 2493 2997 3193 3560 3945 3962 5414 5500 Size: 521 504 196 367 385 17 1452 86 Cuts at: 5500 5512 5858 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 521 504 385 367 346 196 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2211 2211 Size: 5863 BtgI C'CryG_G Cuts at: 513 1065 3094 513 Size: 552 2029 3282 Fragments arranged by size: 3282 2029 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 3151 3379 4173 4193 3151 Size: 228 794 20 4821 Fragments arranged by size: 4821 794 228 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2955 3310 4199 Size: 54 1425 50 275 18 1125 355 889 Cuts at: 4199 5828 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1125 889 355 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2092 2609 2651 2682 3144 3793 1353 Size: 739 517 42 31 462 649 3423 Fragments arranged by size: 3423 739 649 517 462 42 31 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4450 4824 4970 387 Size: 1064 138 2861 374 146 1280 Fragments arranged by size: 2861 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2031 3095 3096 3926 4523 5310 5578 Size: 393 399 1064 1 830 597 787 268 Cuts at: 5578 1239 Size: 1524 Fragments arranged by size: 1524 1064 830 787 597 399 393 268 1 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2569 2569 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4504 4536 4578 4610 1373 Size: 32 135 32 2932 32 42 32 2626 Fragments arranged by size: 2932 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4977 5161 1305 Size: 3672 184 2007 Fragments arranged by size: 3672 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2957 10 Size: 54 1425 343 1125 2916 Fragments arranged by size: 2916 1425 1125 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2765 3093 3821 4180 4339 518 Size: 648 1599 328 728 359 159 2042 Fragments arranged by size: 2042 1599 728 648 359 328 159 MspA1I CmG'CkG Cuts at: 1606 3052 3279 3956 5104 5349 1606 Size: 1446 227 677 1148 245 2120 Fragments arranged by size: 2120 1446 1148 677 245 227 NcoI C'CATG_G Cuts at: 513 1065 3094 513 Size: 552 2029 3282 Fragments arranged by size: 3282 2029 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2239 2809 3221 4766 1132 Size: 97 1010 570 412 1545 2229 Fragments arranged by size: 2229 1545 1010 570 412 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5863 PciI A'CATG_T Cuts at: 2805 4762 2805 Size: 1957 3906 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5863 PfoI T'CCnGG_A Cuts at: 3111 3111 Size: 5863 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3069 3101 3932 3964 5445 5477 3069 Size: 32 831 32 1481 32 3455 Fragments arranged by size: 3455 1481 831 32 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PstI C_TGCA'G Cuts at: 830 3146 3215 830 Size: 2316 69 3478 Fragments arranged by size: 3478 2316 69 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 3052 3052 Size: 5863 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SapI GCTCTTCn'nnn_ Cuts at: 2609 2651 2682 2609 Size: 42 31 5790 Fragments arranged by size: 5790 42 31 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 3142 3211 4346 5027 5218 826 Size: 219 2097 69 1135 681 191 1471 Fragments arranged by size: 2097 1471 1135 681 219 191 69 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 3190 1872 Size: 1318 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2348 2912 3967 4868 5130 820 Size: 197 926 405 564 1055 901 262 1553 Fragments arranged by size: 1553 1055 926 901 564 405 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 3221 1132 Size: 2089 3774 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 2862 1517 Size: 1345 4518 StyI C'CwwG_G Cuts at: 513 1065 2119 2702 2857 3094 3302 513 Size: 552 1054 583 155 237 208 3074 Fragments arranged by size: 3074 1054 583 552 237 208 155 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3890 3907 4060 4245 4690 325 Size: 1255 2310 17 153 185 445 1498 Fragments arranged by size: 2310 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2332 4109 5821 Size: 274 80 33 51 1443 355 1777 1712 Cuts at: 5821 96 Size: 138 Fragments arranged by size: 1777 1712 1443 355 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2294 2326 5823 5855 2294 Size: 32 3497 32 2302 Fragments arranged by size: 3497 2302 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 3188 1870 Size: 1318 4545 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI