################################## # NO CUT ENZYMES ( 129 enzymes) # ################################## Acc65I AccI AccIII AclI AcsI AflII AgeI AhdI AocI Aor51HI ApaI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaII AvrII BamHI BanII BbeI BbrPI BbsI BcgI BciVI BclI BfaI BfrI BglI BlnI BpuAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MaeI MamI MfeI MroI MunI NarI NdeI NheI NotI NruI NspI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SapI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspBI SspI StuI SwaI Tsp509I Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AatII 61 GACGTC GACGT^C AflIII 329 ACGCGT A^C[GA][CT]GT Alw44I 93 GTGCAC G^TGCAC ApaLI 93 GTGCAC G^TGCAC BanI 563 GGCACC G^G[CT][GA]CC BglII 648 AGATCT A^GATCT BlpI 1054 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1054 GCTGAGC GC^T[ACGT]AGC BsaAI 250 TACGTG [CT]AC^GT[GA] BseRI 694 CTCCTC GAGGAG(10/8) BssSI 1199 CACGAG CACGAG(-5/-1) BstAPI 987 GCACGGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CelII 1054 GCTGAGC GC^T[ACGT]AGC DsaI 824 CCATGG C^C[GA][CT]GG EcoT14I 824 CCATGG C^C[AT][AT]GG EcoT22I 814 ATGCAT ATGCA^T HaeII 506 AGCGCC [GA]GCGC^[CT] MboII 47 TCTTC GAAGA(8/7) MluI 329 ACGCGT A^CGCGT MseI 521 TTAA T^TAA MslI 946 CACATCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NaeI 571 GCCGGC GCC^GGC NcoI 824 CCATGG C^CATGG NgoMIV 571 GCCGGC G^CCGGC NsiI 814 ATGCAT ATGCA^T PshAI 880 GACCAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SbfI 192 CCTGCAGG CCTGCA^GG SmaI 200 CCCGGG CCC^GGG Sse8387I 192 CCTGCAGG CCTGCA^GG StyI 824 CCATGG C^C[AT][AT]GG TaqI 275 TCGA T^CGA Tru9I 521 TTAA T^TAA TthHB8I 275 TCGA T^CGA XmaCI 200 CCCGGG C^CCGGG XmaI 200 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AcyI 61 GACGTC G[GA]^CG[CT]C 109 GACGCC AlwI 222 GATCC GGATC(4/5) 255 GATCC AspHI 93 GTGCAC G[AT]GC[AT]^C 1057 GAGCAC BsaHI 61 GACGTC G[GA]^CG[CT]C 109 GACGCC BsaI 4 GAGACC GGTCTC(1/5) 878 GAGACC BsiHKAI 93 GTGCAC G[AT]GC[AT]^C 1057 GAGCAC BsrFI 571 GCCGGC [GA]^CCGG[CT] 1075 ACCGGC BsrI 475 ACTGG ACTGG(1/-1) 417 CCAGT BssHII 78 GCGCGC G^CGCGC 357 GCGCGC BstYI 221 AGATCC [GA]^GATC[CT] 648 AGATCT Cfr10I 571 GCCGGC [GA]^CCGG[CT] 1075 ACCGGC DraII 428 AGGGCCT [GA]G^G[ACGT]CC[CT] 1037 AGGGCCT EcoO109I 428 AGGGCCT [GA]G^G[ACGT]CC[CT] 1037 AGGGCCT FauI 105 GCGGG CCCGC(4/6) 135 GCGGG Hin1I 61 GACGTC G[GA]^CG[CT]C 109 GACGCC MaeII 62 ACGT A^CGT 251 ACGT MflI 221 AGATCC [GA]^GATC[CT] 648 AGATCT PleI 1166 GACTC GAGTC(4/5) 885 GAGTC PstI 193 CTGCAG CTGCA^G 502 CTGCAG PvuII 531 CAGCTG CAG^CTG 1135 CAGCTG TfiI 719 GAATC G^A[AT]TC 1103 GAATC XcmI 903 CCAGGGGCTGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1109 CCAAGCGCGGCCTGG XhoII 221 AGATCC [GA]^GATC[CT] 648 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 71 enzymes) # ######################################### AccII AciI AfaI AluI AlwNI AvaI AviII BalI BbvCI BbvI BcnI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI FokI FspI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeIII MboI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TseI Tsp45I TspRI Van91I