Restriction map for
pFN21AE3969
(Circular) (Six-base) MAPSORT of: pFN21AE3969.seq Check: 6309 from: 1 to: 5959
pFN21AE3969 5959 bp
With 182 enzymes: SgfI *
March 28, 2012 11:54 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2083 2172 2083
Size: 89 5870
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 2070 3769 278
Size: 53 83 186 936 534 1699 2468
Fragments arranged by size:
2468 1699 936 534 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3302 1937
Size: 1365 4594
Acc65I G'GTAC_C
Cuts at: 3280 3280
Size: 5959
AclI AA'CG_TT
Cuts at: 1563 4086 4459 1563
Size: 2523 373 3063
Fragments arranged by size:
3063 2523 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 4022 5406 5674 1632
Size: 2390 1384 268 1917
Fragments arranged by size:
2390 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5762
AflIII A'CryG_T
Cuts at: 1203 2334 4858 5835 1203
Size: 1131 2524 977 1327
Fragments arranged by size:
2524 1327 1131 977
AgeI A'CCGG_T
Cuts at: 4790 4790
Size: 5959
AhdI GACnn_n'nnGTC
Cuts at: 4688 4688
Size: 5959
AleI CACnn'nnGTG
Cuts at: 1166 2955 1166
Size: 1789 4170
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5959
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2849 2881 2849
Size: 32 5927
AlwNI CAG_nnn'CTG
Cuts at: 2230 2301 2715 2893 2914 3702 5274 2230
Size: 71 414 178 21 788 1572 2915
Fragments arranged by size:
2915 1572 788 414 178 71 21
ApaLI G'TGCA_C
Cuts at: 2098 4016 5172 2098
Size: 1918 1156 2885
Fragments arranged by size:
2885 1918 1156
ApoI r'AATT_y
Cuts at: 1493 3268 3494 3646 5755 1493
Size: 1775 226 152 2109 1697
Fragments arranged by size:
2109 1775 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4513 160
Size: 872 3481 1606
Fragments arranged by size:
3481 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
AvaI C'yCGr_G
Cuts at: 1870 1943 2205 2676 2848 3240 3284 1870
Size: 73 262 471 172 392 44 4545
Fragments arranged by size:
4545 471 392 262 172 73 44
BamHI G'GATC_C
Cuts at: 3289 3656 3289
Size: 367 5592
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2568 3280 4735 618
Size: 325 217 627 781 712 1455 1842
Fragments arranged by size:
1842 1455 781 712 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3278 1391
Size: 1887 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4988
BbvCI CC'TCA_GC
Cuts at: 2249 2294 2708 2249
Size: 45 414 5500
Fragments arranged by size:
5500 414 45
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4150 4184 1903
Size: 34 2213 34 3678
Fragments arranged by size:
3678 2213 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3851 5061 3851
Size: 1210 4749
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5959
BfrBI ATG'CAT
Cuts at: 2821 2821
Size: 5959
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4570 136
Size: 107 122 71 631 605 2898 1525
Fragments arranged by size:
2898 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2653 5954 2653
Size: 3301 2658
BlpI GC'TnA_GC
Cuts at: 3060 3376 3060
Size: 316 5643
Bme1580I G_kGCm'C
Cuts at: 1790 2102 2571 4020 5176 1790
Size: 312 469 1449 1156 2573
Fragments arranged by size:
2573 1449 1156 469 312
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5959
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2416 3690 4648
Size: 416 53 83 399 24 999 1274 958
Cuts at: 4648 442
Size: 1753
Fragments arranged by size:
1753 1274 999 958 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5959
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2371 2371
Size: 5959
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2459 2468 2636 3068 3146 3235 4619 1239
Size: 1220 9 168 432 78 89 1384 2579
Fragments arranged by size:
2579 1384 1220 432 168 89 78 9
Bpu10I CC'TnA_GC
Cuts at: 2231 2249 2294 2375 2708 3068 2231
Size: 18 45 81 333 360 5122
Fragments arranged by size:
5122 360 333 81 45 18
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2414 2501 2713 2909 3137 4084 4949 5247 2414
Size: 87 212 196 228 947 865 298 3126
Fragments arranged by size:
3126 947 865 298 228 212 196 87
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2003 2877 4622 882
Size: 617 504 874 1745 2219
Fragments arranged by size:
2219 1745 874 617 504
BsaAI yAC'GTr
Cuts at: 493 1204 2257 3750 493
Size: 711 1053 1493 2702
Fragments arranged by size:
2702 1493 1053 711
BsaBI GATnn'nnATC
Cuts at: 5953 5953
Size: 5959
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2067 2115 3766 4148
Size: 53 83 186 936 534 48 1651 382
Cuts at: 4148 4783 275
Size: 635 1451
Fragments arranged by size:
1651 1451 936 635 534 382 186 83
53 48
BsaWI w'CCGG_w
Cuts at: 4392 4790 5064 5211 5622 4392
Size: 398 274 147 411 4729
Fragments arranged by size:
4729 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2771 2801 1178
Size: 30 165 30 1368 30 4336
Fragments arranged by size:
4336 1368 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2690 1186
Size: 1504 4455
BseYI C'CCAG_C
Cuts at: 2192 2326 2452 5162 5658 2192
Size: 134 126 2710 496 2493
Fragments arranged by size:
2710 2493 496 134 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2144 2243 2363 2729 3085 1163
Size: 573 408 99 120 366 356 4037
Fragments arranged by size:
4037 573 408 366 356 120 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3409 4170 5198 1540
Size: 275 186 1408 761 1028 2301
Fragments arranged by size:
2301 1408 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2102 3066 4020 4105 5176 2102
Size: 964 954 85 1071 2885
Fragments arranged by size:
2885 1071 964 954 85
BsmI GAATG_Cn'
Cuts at: 3470 3563 3470
Size: 93 5866
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2102 2571 3066 3278 4020 4105 5176
Size: 399 312 469 495 212 742 85 1071
Cuts at: 5176 1391
Size: 2174
Fragments arranged by size:
2174 1071 742 495 469 399 312 212
85
BspEI T'CCGG_A
Cuts at: 5622 5622
Size: 5959
BspHI T'CATG_A
Cuts at: 3848 5578 3848
Size: 1730 4229
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2083 2172 2790 3314 844
Size: 1239 89 618 524 3489
Fragments arranged by size:
3489 1239 618 524 89
BsrBI CCG'CTC
Cuts at: 1394 1823 3846 1394
Size: 429 2023 3507
Fragments arranged by size:
3507 2023 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 4454 4628 65
Size: 1183 3206 174 1396
Fragments arranged by size:
3206 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2576 3080 4603 4790 1700
Size: 110 766 504 1523 187 2869
Fragments arranged by size:
2869 1523 766 504 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5959
BssHII G'CGCG_C
Cuts at: 1925 2083 2362 1925
Size: 158 279 5522
Fragments arranged by size:
5522 279 158
BssSI C'ACGA_G
Cuts at: 3204 4019 5031 3204
Size: 815 1012 4132
Fragments arranged by size:
4132 1012 815
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2998 794
Size: 2204 3755
BstBI TT'CG_AA
Cuts at: 4763 4763
Size: 5959
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5959
BstYI r'GATC_y
Cuts at: 1972 2226 2653 3289 3656 4041 4058 5510 5596
Size: 254 427 636 367 385 17 1452 86
Cuts at: 5596 5608 5954 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 636 427 385 367 346 254
86 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 3643
Fragments arranged by size:
3643 939 825 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2479 487
Size: 683 56 1253 3967
Fragments arranged by size:
3967 1253 683 56
BtsI GCAGTG_nn'
Cuts at: 3475 4269 4289 3475
Size: 794 20 5145
Fragments arranged by size:
5145 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5959
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 2003 2038 529
Size: 35 1439 35 4450
Fragments arranged by size:
4450 1439 35 35
DraI TTT'AAA
Cuts at: 3263 3263
Size: 5959
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4966 809
Size: 507 3650 1802
Fragments arranged by size:
3650 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2043 2271 2574
Size: 54 1425 50 275 18 213 228 303
Cuts at: 2574 2826 3406 4295 5924 8
Size: 252 580 889 1629 43
Fragments arranged by size:
1629 1425 889 580 303 275 252 228
213 54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3406 1537
Size: 275 1594 4090
Fragments arranged by size:
4090 1594 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3889 1353
Size: 2536 3423
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2204 2228 2612 4546 4920 5066
Size: 1064 138 615 24 384 1934 374 146
Cuts at: 5066 387
Size: 1280
Fragments arranged by size:
1934 1280 1064 615 384 374 146 138
24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2459 2468 2636 3068 3146 3235 4022
Size: 393 827 9 168 432 78 89 787
Cuts at: 4022 4619 5406 5674 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 827 787 787 597 432 393 268
168 89 78 9
EcoO109I rG'GnC_Cy
Cuts at: 2434 3043 2434
Size: 609 5350
EcoRI G'AATT_C
Cuts at: 3268 3646 3268
Size: 378 5581
FspI TGC'GCA
Cuts at: 2771 3002 3029 4465 2771
Size: 231 27 1436 4265
Fragments arranged by size:
4265 1436 231 27
FspAI rTGC'GCAy
Cuts at: 3029 3029
Size: 5959
HaeII r_GCGC'y
Cuts at: 1678 2515 5106 1678
Size: 837 2591 2531
Fragments arranged by size:
2591 2531 837
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373
Size: 32 135 32 3028 32 42 32 2626
Fragments arranged by size:
3028 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3303 3549 669
Size: 988 281 1365 246 3079
Fragments arranged by size:
3079 1365 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5959
HpaI GTT'AAC
Cuts at: 3549 3549
Size: 5959
KpnI G_GTAC'C
Cuts at: 3284 3284
Size: 5959
MfeI C'AATT_G
Cuts at: 3558 3558
Size: 5959
MluI A'CGCG_T
Cuts at: 2334 5835 2334
Size: 3501 2458
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5073 5257 1305
Size: 3768 184 2007
Fragments arranged by size:
3768 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2045 2273 2828 10
Size: 54 1425 343 213 228 555 3141
Fragments arranged by size:
3141 1425 555 343 228 213 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2955 3917 4276 4435 518
Size: 648 1789 962 359 159 2042
Fragments arranged by size:
2042 1789 962 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2298 2538 3142 3375 4052 5200 5445 1606
Size: 692 240 604 233 677 1148 245 2120
Fragments arranged by size:
2120 1148 692 677 604 245 240 233
NaeI GCC'GGC
Cuts at: 2578 2578
Size: 5959
NcoI C'CATG_G
Cuts at: 513 1065 2004 2829 513
Size: 552 939 825 3643
Fragments arranged by size:
3643 939 825 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5959
NgoMIV G'CCGG_C
Cuts at: 2576 2576
Size: 5959
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5959
NotI GC'GGCC_GC
Cuts at: 3406 3406
Size: 5959
NsiI A_TGCA'T
Cuts at: 2823 2823
Size: 5959
NspI r_CATG'y
Cuts at: 1132 1229 3317 4862 1132
Size: 97 2088 1545 2229
Fragments arranged by size:
2229 2088 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2908 1348
Size: 1560 4399
PciI A'CATG_T
Cuts at: 4858 4858
Size: 5959
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2269 2893 2914 1108
Size: 1161 624 21 4153
Fragments arranged by size:
4153 1161 624 21
PfoI T'CCnGG_A
Cuts at: 2031 2070 2031
Size: 39 5920
PmeI GTTT'AAAC
Cuts at: 3263 3263
Size: 5959
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4028 4060 5541 5573 4028
Size: 32 1481 32 4414
Fragments arranged by size:
4414 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 2889 1613
Size: 1276 4683
PsiI TTA'TAA
Cuts at: 3529 3529
Size: 5959
PstI C_TGCA'G
Cuts at: 830 2202 2511 3311 830
Size: 1372 309 800 3478
Fragments arranged by size:
3478 1372 800 309
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5959
PvuII CAG'CTG
Cuts at: 2538 3142 2538
Size: 604 5355
SalI G'TCGA_C
Cuts at: 1936 3301 1936
Size: 1365 4594
SbfI CC_TGCA'GG
Cuts at: 2202 3311 2202
Size: 1109 4850
ScaI AGT'ACT
Cuts at: 4207 5919 4207
Size: 1712 4247
SfcI C'TryA_G
Cuts at: 826 1045 2198 2507 2630 3307 4442 5123 5314
Size: 219 1153 309 123 677 1135 681 191
Cuts at: 5314 826
Size: 1471
Fragments arranged by size:
1471 1153 1135 681 677 309 219 191
123
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5959
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5959
SmaI CCC'GGG
Cuts at: 1872 2207 3286 1872
Size: 335 1079 4545
Fragments arranged by size:
4545 1079 335
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2429 2516 2692 2924 3152 4063
Size: 197 926 486 87 176 232 228 911
Cuts at: 4063 4964 5226 820
Size: 901 262 1553
Fragments arranged by size:
1553 926 911 901 486 262 232 228
197 176 87
SnaBI TAC'GTA
Cuts at: 493 3750 493
Size: 3257 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5959
SphI G_CATG'C
Cuts at: 1132 3317 1132
Size: 2185 3774
SspI AAT'ATT
Cuts at: 5 52 3883 5
Size: 47 3831 2081
Fragments arranged by size:
3831 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5959
StyI C'CwwG_G
Cuts at: 513 1065 2004 2829 3398 513
Size: 552 939 825 569 3074
Fragments arranged by size:
3074 939 825 569 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3986 4003 4156 4341 4786 325
Size: 1255 2406 17 153 185 445 1498
Fragments arranged by size:
2406 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4205 5917 96
Size: 274 80 33 51 1443 2228 1712 138
Fragments arranged by size:
2228 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3009 3041 5919 5951 3009
Size: 32 2878 32 3017
Fragments arranged by size:
3017 2878 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5959
XbaI T'CTAG_A
Cuts at: 3295 3295
Size: 5959
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2915 3121 2915
Size: 206 5753
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5959
XmaI C'CCGG_G
Cuts at: 1870 2205 3284 1870
Size: 335 1079 4545
Fragments arranged by size:
4545 1079 335
XmnI GAAnn'nnTTC
Cuts at: 4088 4088
Size: 5959
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 2068 3767 276
Size: 53 83 186 936 534 1699 2468
Fragments arranged by size:
2468 1699 936 534 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I
BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI
FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI
NgoMIV NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII
SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI ApaI AscI AvrII BaeI BbeI BsiWI BsmBI
BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI
FseI KasI NarI NruI PacI PmlI PpuMI PspOMI
PspXI PsrI RsrII SacI SacII SanDI SapI SexAI
SfiI SfoI SrfI SwaI