(Linear) (Six-base) MAPSORT of: KIEE3969.seq Check: 9870 from: 1 to: 1254 KIEE3969.orf With 182 enzymes: SgfI * March 22, 2012 17:00 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 78 167 1254 Size: 78 89 1087 Fragments arranged by size: 1087 89 78 AatII G_ACGT'C Cuts at: 0 65 1254 Size: 65 1189 AflIII A'CryG_T Cuts at: 0 329 1254 Size: 329 925 AleI CACnn'nnGTG Cuts at: 0 950 1254 Size: 950 304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 844 876 1254 Size: 844 32 378 Fragments arranged by size: 844 378 32 AlwNI CAG_nnn'CTG Cuts at: 0 225 296 710 888 909 1254 Size: 225 71 414 178 21 345 Fragments arranged by size: 414 345 225 178 71 21 ApaLI G'TGCA_C Cuts at: 0 93 1254 Size: 93 1161 AvaI C'yCGr_G Cuts at: 0 200 671 843 1235 1254 Size: 200 471 172 392 19 Fragments arranged by size: 471 392 200 172 19 BanI G'GyrC_C Cuts at: 0 563 1254 Size: 563 691 BbvCI CC'TCA_GC Cuts at: 0 244 289 703 1254 Size: 244 45 414 551 Fragments arranged by size: 551 414 244 45 BfrBI ATG'CAT Cuts at: 0 816 1254 Size: 816 438 BglII A'GATC_T Cuts at: 0 648 1254 Size: 648 606 BlpI GC'TnA_GC Cuts at: 0 1055 1254 Size: 1055 199 Bme1580I G_kGCm'C Cuts at: 0 97 566 1254 Size: 97 469 688 Fragments arranged by size: 688 469 97 BmrI ACTGGGnnnn_n' Cuts at: 0 411 1254 Size: 411 843 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 366 1254 Size: 366 888 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 463 631 1063 1141 1230 1254 Size: 454 9 168 432 78 89 24 Fragments arranged by size: 454 432 168 89 78 24 9 Bpu10I CC'TnA_GC Cuts at: 0 226 244 289 370 703 1063 1254 Size: 226 18 45 81 333 360 191 Fragments arranged by size: 360 333 226 191 81 45 18 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 409 496 708 904 1132 1254 Size: 409 87 212 196 228 122 Fragments arranged by size: 409 228 212 196 122 87 BsaI GGTCTCn'nnnn_ Cuts at: 0 872 1254 Size: 872 382 BsaAI yAC'GTr Cuts at: 0 252 1254 Size: 252 1002 BsaHI Gr'CG_yC Cuts at: 0 62 110 1254 Size: 62 48 1144 Fragments arranged by size: 1144 62 48 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 766 796 1254 Size: 766 30 458 Fragments arranged by size: 766 458 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 685 1254 Size: 685 569 BseYI C'CCAG_C Cuts at: 0 187 321 447 1254 Size: 187 134 126 807 Fragments arranged by size: 807 187 134 126 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 139 238 358 724 1080 1254 Size: 139 99 120 366 356 174 Fragments arranged by size: 366 356 174 139 120 99 BsiHKAI G_wGCw'C Cuts at: 0 97 1061 1254 Size: 97 964 193 Fragments arranged by size: 964 193 97 Bsp1286I G_dGCh'C Cuts at: 0 97 566 1061 1254 Size: 97 469 495 193 Fragments arranged by size: 495 469 193 97 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 78 167 785 1254 Size: 78 89 618 469 Fragments arranged by size: 618 469 89 78 BsrFI r'CCGG_y Cuts at: 0 571 1075 1254 Size: 571 504 179 Fragments arranged by size: 571 504 179 BssHII G'CGCG_C Cuts at: 0 78 357 1254 Size: 78 279 897 Fragments arranged by size: 897 279 78 BssSI C'ACGA_G Cuts at: 0 1199 1254 Size: 1199 55 BstAPI GCAn_nnn'nTGC Cuts at: 0 993 1254 Size: 993 261 BstYI r'GATC_y Cuts at: 0 221 648 1254 Size: 221 427 606 Fragments arranged by size: 606 427 221 BtgI C'CryG_G Cuts at: 0 824 1254 Size: 824 430 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 474 1254 Size: 474 780 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 33 1254 Size: 33 1221 EaeI y'GGCC_r Cuts at: 0 38 266 569 821 1254 Size: 38 228 303 252 433 Fragments arranged by size: 433 303 252 228 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 199 223 607 1254 Size: 199 24 384 647 Fragments arranged by size: 647 384 199 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 454 463 631 1063 1141 1230 1254 Size: 454 9 168 432 78 89 24 Fragments arranged by size: 454 432 168 89 78 24 9 EcoO109I rG'GnC_Cy Cuts at: 0 429 1038 1254 Size: 429 609 216 Fragments arranged by size: 609 429 216 FspI TGC'GCA Cuts at: 0 766 997 1024 1254 Size: 766 231 27 230 Fragments arranged by size: 766 231 230 27 FspAI rTGC'GCAy Cuts at: 0 1024 1254 Size: 1024 230 HaeII r_GCGC'y Cuts at: 0 510 1254 Size: 510 744 MluI A'CGCG_T Cuts at: 0 329 1254 Size: 329 925 MscI TGG'CCA Cuts at: 0 40 268 823 1254 Size: 40 228 555 431 Fragments arranged by size: 555 431 228 40 MslI CAynn'nnrTG Cuts at: 0 950 1254 Size: 950 304 MspA1I CmG'CkG Cuts at: 0 293 533 1137 1254 Size: 293 240 604 117 Fragments arranged by size: 604 293 240 117 NaeI GCC'GGC Cuts at: 0 573 1254 Size: 573 681 NcoI C'CATG_G Cuts at: 0 824 1254 Size: 824 430 NgoMIV G'CCGG_C Cuts at: 0 571 1254 Size: 571 683 NsiI A_TGCA'T Cuts at: 0 818 1254 Size: 818 436 PasI CC'CwG_GG Cuts at: 0 903 1254 Size: 903 351 PflMI CCAn_nnn'nTGG Cuts at: 0 264 888 909 1254 Size: 264 624 21 345 Fragments arranged by size: 624 345 264 21 PfoI T'CCnGG_A Cuts at: 0 26 65 1254 Size: 26 39 1189 Fragments arranged by size: 1189 39 26 PshAI GACnn'nnGTC Cuts at: 0 884 1254 Size: 884 370 PstI C_TGCA'G Cuts at: 0 197 506 1254 Size: 197 309 748 Fragments arranged by size: 748 309 197 PvuII CAG'CTG Cuts at: 0 533 1137 1254 Size: 533 604 117 Fragments arranged by size: 604 533 117 SbfI CC_TGCA'GG Cuts at: 0 197 1254 Size: 197 1057 SfcI C'TryA_G Cuts at: 0 193 502 625 1254 Size: 193 309 123 629 Fragments arranged by size: 629 309 193 123 SmaI CCC'GGG Cuts at: 0 202 1254 Size: 202 1052 SmlI C'TyrA_G Cuts at: 0 424 511 687 919 1147 1254 Size: 424 87 176 232 228 107 Fragments arranged by size: 424 232 228 176 107 87 StyI C'CwwG_G Cuts at: 0 824 1254 Size: 824 430 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1004 1036 1254 Size: 1004 32 218 Fragments arranged by size: 1004 218 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 910 1116 1254 Size: 910 206 138 Fragments arranged by size: 910 206 138 XmaI C'CCGG_G Cuts at: 0 200 1254 Size: 200 1054 ZraI GAC'GTC Cuts at: 0 63 1254 Size: 63 1191 Enzymes that do cut: AarI AatII AflIII AleI AloI AlwNI ApaLI AvaI BanI BbvCI BfrBI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I BspMI BsrFI BssHII BssSI BstAPI BstYI BtgI BtgZI CspCI EaeI EciI Eco57MI EcoO109I FspI FspAI HaeII MluI MscI MslI MspA1I NaeI NcoI NgoMIV NsiI PasI PflMI PfoI PshAI PstI PvuII SbfI SfcI SmaI SmlI StyI TstI XcmI XmaI ZraI Enzymes that do not cut: AccI Acc65I AclI AcuI AfeI AflII AgeI AhdI AlfI ApaI ApoI AscI AseI AsiSI AvrII BaeI BamHI BanII BbeI BbsI BcgI BciVI BclI BglI BmgBI BmtI BsaBI BsaWI BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrDI BsrGI BstBI BstEII BstXI BstZ17I Bsu36I BtsI ClaI DraI DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI Hin4I HincII HindIII HpaI KasI KpnI MfeI MmeI NarI NdeI NheI NotI NruI NspI PacI PciI PmeI PmlI PpiI PpuMI PsiI PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SapI ScaI SexAI SfiI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI Tth111I XbaI XhoI XmnI