################################## # NO CUT ENZYMES ( 107 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII BamHI BbeI BbrPI BbvCI BcgI BfrI BglI BlpI Bpu1102I BsaAI BsaHI BsgI BsiEI BsiWI BsmI BspDI BspHI BssHII BssSI Bst1107I BstBI BstZ17I CelII ClaI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI KspI MfeI MluI MseI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PstI PvuI RcaI SacII SalI SbfI ScaI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspI StuI SwaI TfiI Tru9I Tth111I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 57 enzymes) # ################################### AccI 391 GTCTAC GT^[AC][GT]AC AccII 1261 CGCG CG^CG AccIII 1244 TCCGGA T^CCGGA AflIII 1226 ACATGT A^C[GA][CT]GT AhdI 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AlwI 102 GGATC GGATC(4/5) AlwNI 1073 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 841 CCTCAGG CC^T[ACGT]AGG ApaI 1789 GGGCCC GGGCC^C AspEI 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspHI 1264 GAGCTC G[AT]GC[AT]^C AvrII 1170 CCTAGG C^CTAGG BalI 1529 TGGCCA TGG^CCA BciVI 1603 GGATAC GTATCC(6/5) BclI 761 TGATCA T^GATCA BfaI 1171 CTAG C^TAG BlnI 1170 CCTAGG C^CTAGG BsaBI 1332 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 1244 TCCGGA [AT]^CCGG[AT] BseAI 1244 TCCGGA T^CCGGA BsiHKAI 1264 GAGCTC G[AT]GC[AT]^C BsmBI 579 GAGACG CGTCTC(1/5) Bsp1407I 1334 TGTACA T^GTACA BspEI 1244 TCCGGA T^CCGGA BspLU11I 1226 ACATGT A^CATGT BspMI 466 ACCTGC ACCTGC(4/8) BsrBI 1251 GAGCGG CCGCTC(-3/-3) BsrGI 1334 TGTACA T^GTACA BstAPI 1041 GCAAGGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1317 GGTCACC G^GT[ACGT]ACC BstPI 1317 GGTCACC G^GT[ACGT]ACC BstUI 1261 CGCG CG^CG Bsu36I 841 CCTCAGG CC^T[ACGT]AGG CpoI 1433 CGGACCG CG^G[AT]CCG Eam1105I 1680 GACTCGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 841 CCTCAGG CC^T[ACGT]AGG EcoNI 339 CCTCTTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1317 GGTCACC G^GT[ACGT]ACC EcoRI 568 GAATTC G^AATTC FbaI 761 TGATCA T^GATCA MaeI 1171 CTAG C^TAG MamI 1332 GATGTACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 1529 TGGCCA TGG^CCA MroI 1244 TCCGGA T^CCGGA MscI 1529 TGGCCA TGG^CCA MvnI 1261 CGCG CG^CG NaeI 523 GCCGGC GCC^GGC NdeI 1374 CATATG CA^TATG NgoMIV 523 GCCGGC G^CCGGC NspI 1226 ACATGT [GA]CATG^[CT] PshAI 1726 GACTCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 1433 CGGACCG CG^G[AT]CCG SacI 1264 GAGCTC GAGCT^C SexAI 92 ACCAGGT A^CC[AT]GGT SspBI 1334 TGTACA T^GTACA TaqI 374 TCGA T^CGA TthHB8I 374 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 546 GAATTT [GA]^AATT[CT] 568 GAATTC ApoI 546 GAATTT [GA]^AATT[CT] 568 GAATTC BsaI 1831 GAGACC GGTCTC(1/5) 42 GGTCTC BseRI 1267 CTCCTC GAGGAG(10/8) 58 GAGGAG BsrDI 493 CATTGC GCAATG(2/0) 170 GCAATG BsrFI 523 GCCGGC [GA]^CCGG[CT] 1472 ACCGGC CfoI 627 GCGC GCG^C 1260 GCGC Cfr10I 523 GCCGGC [GA]^CCGG[CT] 1472 ACCGGC DsaI 1477 CCATGG C^C[GA][CT]GG 1637 CCATGG HgaI 1701 GCGTC GACGC(5/10) 1707 GCGTC HhaI 627 GCGC GCG^C 1260 GCGC HinP1I 627 GCGC G^CGC 1260 GCGC MaeII 730 ACGT A^CGT 1580 ACGT MslI 258 CATCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1221 CATAGACATG MspA1I 1132 CAGCTG C[AC]G^C[GT]G 1345 CAGCTG NcoI 1477 CCATGG C^CATGG 1637 CCATGG PflMI 609 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1610 CCATTGGCTGG PvuII 1132 CAGCTG CAG^CTG 1345 CAGCTG SapI 1248 GAAGAGC GCTCTTC(1/4) 1503 GCTCTTC Tsp509I 547 AATT ^AATT 569 AATT Van91I 609 CCATGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1610 CCATTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 73 enzymes) # ######################################### AciI AfaI AluI AvaI AvaII BanI BanII BbsI BbvI BcnI BglII BmyI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeIII MboI MboII MflI MnlI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SmlI StyI TseI Tsp45I TspRI XcmI XhoII