(Circular) (Six-base) MAPSORT of: pFN21AE6466.seq Check: 6314 from: 1 to: 6547 pFN21AE6466 6547 bp With 182 enzymes: SgfI * March 18, 2015 14:52 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4357 278 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2397 3890 1937 Size: 460 1493 4594 Fragments arranged by size: 4594 1493 460 Acc65I G'GTAC_C Cuts at: 3868 3868 Size: 6547 AclI AA'CG_TT Cuts at: 1563 4674 5047 1563 Size: 3111 373 3063 Fragments arranged by size: 3111 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3084 3701 4610 5994 6262 1632 Size: 1452 617 909 1384 268 1917 Fragments arranged by size: 1917 1452 1384 909 617 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6350 AflIII A'CryG_T Cuts at: 1203 3231 5446 6423 1203 Size: 2028 2215 977 1327 Fragments arranged by size: 2215 2028 1327 977 AgeI A'CCGG_T Cuts at: 5378 5378 Size: 6547 AhdI GACnn_n'nnGTC Cuts at: 3690 5276 3690 Size: 1586 4961 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6547 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3401 1184 Size: 2217 4330 AlwNI CAG_nnn'CTG Cuts at: 3083 4290 5862 3083 Size: 1207 1572 3768 Fragments arranged by size: 3768 1572 1207 ApaI G_GGCC'C Cuts at: 3798 3798 Size: 6547 ApaLI G'TGCA_C Cuts at: 4604 5760 4604 Size: 1156 5391 ApoI r'AATT_y Cuts at: 1493 2551 2573 3856 4082 4234 6343 1493 Size: 1058 22 1283 226 152 2109 1697 Fragments arranged by size: 2109 1697 1283 1058 226 152 22 AseI AT'TA_AT Cuts at: 160 1032 5101 160 Size: 872 4069 1606 Fragments arranged by size: 4069 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 AvaI C'yCGr_G Cuts at: 1870 1943 2060 2605 3687 3872 1870 Size: 73 117 545 1082 185 4545 Fragments arranged by size: 4545 1082 545 185 117 73 AvrII C'CTAG_G Cuts at: 3175 3175 Size: 6547 BamHI G'GATC_C Cuts at: 3877 4244 3877 Size: 367 6180 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2867 2904 3052 3868 5323 Size: 325 217 627 1080 37 148 816 1455 Cuts at: 5323 618 Size: 1842 Fragments arranged by size: 1842 1455 1080 816 627 325 217 148 37 BanII G_rGCy'C Cuts at: 1391 2425 2627 3273 3798 3866 1391 Size: 1034 202 646 525 68 4072 Fragments arranged by size: 4072 1034 646 525 202 68 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2094 3007 3689 928 Size: 971 195 913 682 3786 Fragments arranged by size: 3786 971 913 682 195 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4738 4772 1903 Size: 34 2801 34 3678 Fragments arranged by size: 3678 2801 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3602 4439 5649 3602 Size: 837 1210 4500 Fragments arranged by size: 4500 1210 837 BclI T'GATC_A Cuts at: 1548 2766 1548 Size: 1218 5329 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5158 136 Size: 107 122 71 631 605 3486 1525 Fragments arranged by size: 3486 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2334 2874 3498 6542 2334 Size: 540 624 3044 2339 Fragments arranged by size: 3044 2339 624 540 BlpI GC'TnA_GC Cuts at: 3964 3964 Size: 6547 Bme1580I G_kGCm'C Cuts at: 1790 2870 3057 3798 4608 5764 1790 Size: 1080 187 741 810 1156 2573 Fragments arranged by size: 2573 1156 1080 810 741 187 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6547 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4278 5236 442 Size: 416 53 83 399 24 2861 958 1753 Fragments arranged by size: 2861 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6547 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2203 2300 2347 3718 5207 1239 Size: 964 97 47 1371 1489 2579 Fragments arranged by size: 2579 1489 1371 964 97 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2822 3557 3574 4672 5537 5835 2822 Size: 735 17 1098 865 298 3534 Fragments arranged by size: 3534 1098 865 735 298 17 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2053 3830 5210 882 Size: 617 554 1777 1380 2219 Fragments arranged by size: 2219 1777 1380 617 554 BsaAI yAC'GTr Cuts at: 493 1204 4338 493 Size: 711 3134 2702 Fragments arranged by size: 3134 2702 711 BsaBI GATnn'nnATC Cuts at: 3341 6541 3341 Size: 3200 3347 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4354 4736 5371 275 Size: 53 83 186 936 2821 382 635 1451 Fragments arranged by size: 2821 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3249 4980 5378 5652 5799 6210 3249 Size: 1731 398 274 147 411 3586 Fragments arranged by size: 3586 1731 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2273 2303 2609 2639 1178 Size: 30 165 30 870 30 306 30 5086 Fragments arranged by size: 5086 870 306 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2078 3263 1186 Size: 892 1185 4470 Fragments arranged by size: 4470 1185 892 BseYI C'CCAG_C Cuts at: 2170 2634 3002 3135 3423 5750 6246 2170 Size: 464 368 133 288 2327 496 2471 Fragments arranged by size: 2471 2327 496 464 368 288 133 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5974 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3997 4758 5786 1540 Size: 275 186 1996 761 1028 2301 Fragments arranged by size: 2301 1996 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3273 4608 4693 5764 3273 Size: 1335 85 1071 4056 Fragments arranged by size: 4056 1335 1071 85 BsmI GAATG_Cn' Cuts at: 4058 4151 4058 Size: 93 6454 BsmBI CGTCTCn'nnnn_ Cuts at: 2578 2578 Size: 6547 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2425 2627 2870 3057 3273 3798 3866 Size: 399 635 202 243 187 216 525 68 Cuts at: 3866 4608 4693 5764 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 635 525 399 243 216 202 187 85 68 BspEI T'CCGG_A Cuts at: 3249 6210 3249 Size: 2961 3586 BspHI T'CATG_A Cuts at: 4436 6166 4436 Size: 1730 4817 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2480 3902 844 Size: 1636 1422 3489 Fragments arranged by size: 3489 1636 1422 BsrBI CCG'CTC Cuts at: 1394 1823 3258 4434 1394 Size: 429 1435 1176 3507 Fragments arranged by size: 3507 1435 1176 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2182 2497 5042 5216 65 Size: 1183 934 315 2545 174 1396 Fragments arranged by size: 2545 1396 1183 934 315 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2528 3477 5191 5378 1700 Size: 110 718 949 1714 187 2869 Fragments arranged by size: 2869 1714 949 718 187 110 BsrGI T'GTAC_A Cuts at: 96 3339 96 Size: 3243 3304 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6547 BssSI C'ACGA_G Cuts at: 4607 5619 4607 Size: 1012 5535 BstAPI GCAn_nnn'nTGC Cuts at: 794 3052 794 Size: 2258 4289 BstBI TT'CG_AA Cuts at: 5351 5351 Size: 6547 BstEII G'GTnAC_C Cuts at: 1184 3322 1184 Size: 2138 4409 BstXI CCAn_nnnn'nTGG Cuts at: 2131 2357 2998 2131 Size: 226 641 5680 Fragments arranged by size: 5680 641 226 BstYI r'GATC_y Cuts at: 1972 2334 2874 3498 3877 4244 4629 4646 6098 Size: 362 540 624 379 367 385 17 1452 Cuts at: 6098 6184 6196 6542 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 624 540 385 379 367 362 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2847 2847 Size: 6547 BtgI C'CryG_G Cuts at: 513 1065 2004 3482 3642 513 Size: 552 939 1478 160 3418 Fragments arranged by size: 3418 1478 939 552 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2947 3082 3271 487 Size: 683 56 1721 135 189 3763 Fragments arranged by size: 3763 1721 683 189 135 56 BtsI GCAGTG_nn' Cuts at: 2028 3781 4063 4857 4877 2028 Size: 1753 282 794 20 3698 Fragments arranged by size: 3698 1753 794 282 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6547 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2977 3012 529 Size: 35 2413 35 4064 Fragments arranged by size: 4064 2413 35 35 DraI TTT'AAA Cuts at: 3851 3851 Size: 6547 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5554 809 Size: 507 4238 1802 Fragments arranged by size: 4238 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2151 3069 3479 Size: 54 1425 50 275 18 321 918 410 Cuts at: 3479 3534 3781 3994 4883 6512 8 Size: 55 247 213 889 1629 43 Fragments arranged by size: 1629 1425 918 889 410 321 275 247 213 55 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3994 1537 Size: 275 2182 4090 Fragments arranged by size: 4090 2182 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2351 2432 3248 3515 3551 3665 4477 1353 Size: 998 81 816 267 36 114 812 3423 Fragments arranged by size: 3423 998 816 812 267 114 81 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2822 3452 5134 5508 5654 387 Size: 1064 138 1233 630 1682 374 146 1280 Fragments arranged by size: 1682 1280 1233 1064 630 374 146 138 EcoICRI GAG'CTC Cuts at: 3271 3271 Size: 6547 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2203 2300 2347 3084 3701 3718 4610 Size: 393 571 97 47 737 617 17 892 Cuts at: 4610 5207 5994 6262 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 892 787 737 617 597 571 393 268 97 47 17 EcoNI CCTnn'n_nnAGG Cuts at: 2348 2348 Size: 6547 EcoO109I rG'GnC_Cy Cuts at: 2056 2842 3080 3692 3795 2056 Size: 786 238 612 103 4808 Fragments arranged by size: 4808 786 612 238 103 EcoRI G'AATT_C Cuts at: 2573 3856 4234 2573 Size: 1283 378 4886 Fragments arranged by size: 4886 1283 378 FspI TGC'GCA Cuts at: 5053 5053 Size: 6547 HaeII r_GCGC'y Cuts at: 1678 5694 1678 Size: 4016 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2609 2641 3676 3708 5188 Size: 32 135 32 1037 32 1035 32 1480 Cuts at: 5188 5220 5262 5294 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1480 1037 1035 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3891 4137 669 Size: 988 281 1953 246 3079 Fragments arranged by size: 3079 1953 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6547 HpaI GTT'AAC Cuts at: 4137 4137 Size: 6547 KpnI G_GTAC'C Cuts at: 3872 3872 Size: 6547 MfeI C'AATT_G Cuts at: 4146 4146 Size: 6547 MluI A'CGCG_T Cuts at: 6423 6423 Size: 6547 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2058 5661 5845 1305 Size: 753 3603 184 2007 Fragments arranged by size: 3603 2007 753 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3536 10 Size: 54 1425 343 1704 3021 Fragments arranged by size: 3021 1704 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2267 3230 4505 4864 5023 518 Size: 648 1101 963 1275 359 159 2042 Fragments arranged by size: 2042 1275 1101 963 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3139 3352 3963 4640 5788 6033 1606 Size: 1533 213 611 677 1148 245 2120 Fragments arranged by size: 2120 1533 1148 677 611 245 213 NaeI GCC'GGC Cuts at: 2530 2530 Size: 6547 NcoI C'CATG_G Cuts at: 513 1065 2004 3482 3642 513 Size: 552 939 1478 160 3418 Fragments arranged by size: 3418 1478 939 552 160 NdeI CA'TA_TG Cuts at: 387 3380 387 Size: 2993 3554 NgoMIV G'CCGG_C Cuts at: 2528 2528 Size: 6547 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6547 NotI GC'GGCC_GC Cuts at: 3994 3994 Size: 6547 NspI r_CATG'y Cuts at: 1132 1229 3235 3905 5450 1132 Size: 97 2006 670 1545 2229 Fragments arranged by size: 2229 2006 1545 670 97 PasI CC'CwG_GG Cuts at: 1348 2917 3603 1348 Size: 1569 686 4292 Fragments arranged by size: 4292 1569 686 PciI A'CATG_T Cuts at: 3231 5446 3231 Size: 2215 4332 PflMI CCAn_nnn'nTGG Cuts at: 1108 2620 3621 1108 Size: 1512 1001 4034 Fragments arranged by size: 4034 1512 1001 PfoI T'CCnGG_A Cuts at: 2277 2785 2277 Size: 508 6039 PmeI GTTT'AAAC Cuts at: 3851 3851 Size: 6547 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4616 4648 6129 6161 4616 Size: 32 1481 32 5002 Fragments arranged by size: 5002 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2056 2842 3080 3692 2056 Size: 786 238 612 4911 Fragments arranged by size: 4911 786 612 238 PshAI GACnn'nnGTC Cuts at: 1613 3735 1613 Size: 2122 4425 PsiI TTA'TAA Cuts at: 4117 4117 Size: 6547 PspOMI G'GGCC_C Cuts at: 3794 3794 Size: 6547 PstI C_TGCA'G Cuts at: 830 3899 830 Size: 3069 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6547 PvuII CAG'CTG Cuts at: 3139 3352 3139 Size: 213 6334 RsrII CG'GwC_CG Cuts at: 3439 3439 Size: 6547 SacI G_AGCT'C Cuts at: 3273 3273 Size: 6547 SalI G'TCGA_C Cuts at: 1936 3889 1936 Size: 1953 4594 SanDI GG'GwC_CC Cuts at: 2056 2056 Size: 6547 SapI GCTCTTCn'nnn_ Cuts at: 3248 3515 3248 Size: 267 6280 SbfI CC_TGCA'GG Cuts at: 3899 3899 Size: 6547 ScaI AGT'ACT Cuts at: 4795 6507 4795 Size: 1712 4835 SexAI A'CCwGG_T Cuts at: 2097 2097 Size: 6547 SfcI C'TryA_G Cuts at: 826 1045 3895 5030 5711 5902 826 Size: 219 2850 1135 681 191 1471 Fragments arranged by size: 2850 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6547 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6547 SmaI CCC'GGG Cuts at: 1872 3874 1872 Size: 2002 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2837 3553 3572 4651 5552 5814 Size: 197 926 894 716 19 1079 901 262 Cuts at: 5814 820 Size: 1553 Fragments arranged by size: 1553 1079 926 901 894 716 262 197 19 SnaBI TAC'GTA Cuts at: 493 4338 493 Size: 3845 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6547 SphI G_CATG'C Cuts at: 1132 3905 1132 Size: 2773 3774 SspI AAT'ATT Cuts at: 5 52 4471 5 Size: 47 4419 2081 Fragments arranged by size: 4419 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6547 StyI C'CwwG_G Cuts at: 513 1065 2004 2308 3108 3175 3482 3642 3986 Size: 552 939 304 800 67 307 160 344 Cuts at: 3986 513 Size: 3074 Fragments arranged by size: 3074 939 800 552 344 307 304 160 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2885 3415 4574 4591 4744 4929 5374 Size: 1255 1305 530 1159 17 153 185 445 Cuts at: 5374 325 Size: 1498 Fragments arranged by size: 1498 1305 1255 1159 530 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3339 3561 3576 Size: 274 80 33 51 1443 1362 222 15 Cuts at: 3576 4793 6505 96 Size: 1217 1712 138 Fragments arranged by size: 1712 1443 1362 1217 274 222 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6507 6539 6507 Size: 32 6515 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6547 XbaI T'CTAG_A Cuts at: 3883 3883 Size: 6547 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2713 2995 3064 2713 Size: 282 69 6196 Fragments arranged by size: 6196 282 69 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6547 XmaI C'CCGG_G Cuts at: 1870 3872 1870 Size: 2002 4545 XmnI GAAnn'nnTTC Cuts at: 4676 4676 Size: 6547 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4355 276 Size: 53 83 186 936 2821 2468 Fragments arranged by size: 2821 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstZ17I DraIII EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI SacII SfiI SfoI SrfI SwaI