(Linear) (Six-base) MAPSORT of: KIEE6466.seq Check: 7507 from: 1 to: 1842 KIEE6466.orf With 182 enzymes: SgfI * March 16, 2015 11:32 .. AccI GT'mk_AC Cuts at: 0 392 1842 Size: 392 1450 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1079 1696 1842 Size: 1079 617 146 Fragments arranged by size: 1079 617 146 AflIII A'CryG_T Cuts at: 0 1226 1842 Size: 1226 616 AhdI GACnn_n'nnGTC Cuts at: 0 1685 1842 Size: 1685 157 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 1396 1842 Size: 1396 446 AlwNI CAG_nnn'CTG Cuts at: 0 1078 1842 Size: 1078 764 ApaI G_GGCC'C Cuts at: 0 1793 1842 Size: 1793 49 ApoI r'AATT_y Cuts at: 0 546 568 1842 Size: 546 22 1274 Fragments arranged by size: 1274 546 22 AvaI C'yCGr_G Cuts at: 0 55 600 1682 1842 Size: 55 545 1082 160 Fragments arranged by size: 1082 545 160 55 AvrII C'CTAG_G Cuts at: 0 1170 1842 Size: 1170 672 BanI G'GyrC_C Cuts at: 0 862 899 1047 1842 Size: 862 37 148 795 Fragments arranged by size: 862 795 148 37 BanII G_rGCy'C Cuts at: 0 420 622 1268 1793 1842 Size: 420 202 646 525 49 Fragments arranged by size: 646 525 420 202 49 BbsI GAAGACnn'nnnn_ Cuts at: 0 89 1002 1684 1842 Size: 89 913 682 158 Fragments arranged by size: 913 682 158 89 BciVI GTATCCnnnnn_n' Cuts at: 0 1597 1842 Size: 1597 245 BclI T'GATC_A Cuts at: 0 761 1842 Size: 761 1081 BglII A'GATC_T Cuts at: 0 329 869 1493 1842 Size: 329 540 624 349 Fragments arranged by size: 624 540 349 329 Bme1580I G_kGCm'C Cuts at: 0 865 1052 1793 1842 Size: 865 187 741 49 Fragments arranged by size: 865 741 187 49 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 295 342 1713 1842 Size: 198 97 47 1371 129 Fragments arranged by size: 1371 198 129 97 47 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 817 1552 1569 1842 Size: 817 735 17 273 Fragments arranged by size: 817 735 273 17 BsaI GGTCTCn'nnnn_ Cuts at: 0 48 1825 1842 Size: 48 1777 17 Fragments arranged by size: 1777 48 17 BsaBI GATnn'nnATC Cuts at: 0 1336 1842 Size: 1336 506 BsaWI w'CCGG_w Cuts at: 0 1244 1842 Size: 1244 598 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 268 298 604 634 1842 Size: 268 30 306 30 1208 Fragments arranged by size: 1208 306 268 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 73 1258 1842 Size: 73 1185 584 Fragments arranged by size: 1185 584 73 BseYI C'CCAG_C Cuts at: 0 165 629 997 1130 1418 1842 Size: 165 464 368 133 288 424 Fragments arranged by size: 464 424 368 288 165 133 BsiHKAI G_wGCw'C Cuts at: 0 1268 1842 Size: 1268 574 BsmBI CGTCTCn'nnnn_ Cuts at: 0 573 1842 Size: 573 1269 Bsp1286I G_dGCh'C Cuts at: 0 420 622 865 1052 1268 1793 1842 Size: 420 202 243 187 216 525 49 Fragments arranged by size: 525 420 243 216 202 187 49 BspEI T'CCGG_A Cuts at: 0 1244 1842 Size: 1244 598 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 475 1842 Size: 475 1367 BsrBI CCG'CTC Cuts at: 0 1253 1842 Size: 1253 589 BsrDI GCAATG_nn' Cuts at: 0 177 492 1842 Size: 177 315 1350 Fragments arranged by size: 1350 315 177 BsrFI r'CCGG_y Cuts at: 0 523 1472 1842 Size: 523 949 370 Fragments arranged by size: 949 523 370 BsrGI T'GTAC_A Cuts at: 0 1334 1842 Size: 1334 508 BstAPI GCAn_nnn'nTGC Cuts at: 0 1047 1842 Size: 1047 795 BstEII G'GTnAC_C Cuts at: 0 1317 1842 Size: 1317 525 BstXI CCAn_nnnn'nTGG Cuts at: 0 126 352 993 1842 Size: 126 226 641 849 Fragments arranged by size: 849 641 226 126 BstYI r'GATC_y Cuts at: 0 329 869 1493 1842 Size: 329 540 624 349 Fragments arranged by size: 624 540 349 329 Bsu36I CC'TnA_GG Cuts at: 0 842 1842 Size: 842 1000 BtgI C'CryG_G Cuts at: 0 1477 1637 1842 Size: 1477 160 205 Fragments arranged by size: 1477 205 160 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 942 1077 1266 1842 Size: 942 135 189 576 Fragments arranged by size: 942 576 189 135 BtsI GCAGTG_nn' Cuts at: 0 23 1776 1842 Size: 23 1753 66 Fragments arranged by size: 1753 66 23 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 972 1007 1842 Size: 972 35 835 Fragments arranged by size: 972 835 35 EaeI y'GGCC_r Cuts at: 0 146 1064 1474 1529 1776 1842 Size: 146 918 410 55 247 66 Fragments arranged by size: 918 410 247 146 66 55 EarI CTCTTCn'nnn_ Cuts at: 0 346 427 1243 1510 1546 1660 1842 Size: 346 81 816 267 36 114 182 Fragments arranged by size: 816 346 267 182 114 81 36 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 817 1447 1842 Size: 817 630 395 Fragments arranged by size: 817 630 395 EcoICRI GAG'CTC Cuts at: 0 1266 1842 Size: 1266 576 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 198 295 342 1079 1696 1713 1842 Size: 198 97 47 737 617 17 129 Fragments arranged by size: 737 617 198 129 97 47 17 EcoNI CCTnn'n_nnAGG Cuts at: 0 343 1842 Size: 343 1499 EcoO109I rG'GnC_Cy Cuts at: 0 51 837 1075 1687 1790 1842 Size: 51 786 238 612 103 52 Fragments arranged by size: 786 612 238 103 52 51 EcoRI G'AATT_C Cuts at: 0 568 1842 Size: 568 1274 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 604 636 1671 1703 1842 Size: 604 32 1035 32 139 Fragments arranged by size: 1035 604 139 32 32 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 53 1842 Size: 53 1789 MscI TGG'CCA Cuts at: 0 1531 1842 Size: 1531 311 MslI CAynn'nnrTG Cuts at: 0 262 1225 1842 Size: 262 963 617 Fragments arranged by size: 963 617 262 MspA1I CmG'CkG Cuts at: 0 1134 1347 1842 Size: 1134 213 495 Fragments arranged by size: 1134 495 213 NaeI GCC'GGC Cuts at: 0 525 1842 Size: 525 1317 NcoI C'CATG_G Cuts at: 0 1477 1637 1842 Size: 1477 160 205 Fragments arranged by size: 1477 205 160 NdeI CA'TA_TG Cuts at: 0 1375 1842 Size: 1375 467 NgoMIV G'CCGG_C Cuts at: 0 523 1842 Size: 523 1319 NspI r_CATG'y Cuts at: 0 1230 1842 Size: 1230 612 PasI CC'CwG_GG Cuts at: 0 912 1598 1842 Size: 912 686 244 Fragments arranged by size: 912 686 244 PciI A'CATG_T Cuts at: 0 1226 1842 Size: 1226 616 PflMI CCAn_nnn'nTGG Cuts at: 0 615 1616 1842 Size: 615 1001 226 Fragments arranged by size: 1001 615 226 PfoI T'CCnGG_A Cuts at: 0 272 780 1842 Size: 272 508 1062 Fragments arranged by size: 1062 508 272 PpuMI rG'GwC_Cy Cuts at: 0 51 837 1075 1687 1842 Size: 51 786 238 612 155 Fragments arranged by size: 786 612 238 155 51 PshAI GACnn'nnGTC Cuts at: 0 1730 1842 Size: 1730 112 PspOMI G'GGCC_C Cuts at: 0 1789 1842 Size: 1789 53 PvuII CAG'CTG Cuts at: 0 1134 1347 1842 Size: 1134 213 495 Fragments arranged by size: 1134 495 213 RsrII CG'GwC_CG Cuts at: 0 1434 1842 Size: 1434 408 SacI G_AGCT'C Cuts at: 0 1268 1842 Size: 1268 574 SanDI GG'GwC_CC Cuts at: 0 51 1842 Size: 51 1791 SapI GCTCTTCn'nnn_ Cuts at: 0 1243 1510 1842 Size: 1243 267 332 Fragments arranged by size: 1243 332 267 SexAI A'CCwGG_T Cuts at: 0 92 1842 Size: 92 1750 SmlI C'TyrA_G Cuts at: 0 832 1548 1567 1842 Size: 832 716 19 275 Fragments arranged by size: 832 716 275 19 StyI C'CwwG_G Cuts at: 0 303 1103 1170 1477 1637 1842 Size: 303 800 67 307 160 205 Fragments arranged by size: 800 307 303 205 160 67 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 880 1410 1842 Size: 880 530 432 Fragments arranged by size: 880 530 432 TatI w'GTAC_w Cuts at: 0 1334 1556 1571 1842 Size: 1334 222 15 271 Fragments arranged by size: 1334 271 222 15 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 708 990 1059 1842 Size: 708 282 69 783 Fragments arranged by size: 783 708 282 69 Enzymes that do cut: AccI AcuI AflIII AhdI AlfI AlwNI ApaI ApoI AvaI AvrII BanI BanII BbsI BciVI BclI BglII Bme1580I BpmI BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BsrGI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI Hin4I MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NspI PasI PciI PflMI PfoI PpuMI PshAI PspOMI PvuII RsrII SacI SanDI SapI SexAI SmlI StyI TaqII TatI XcmI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI AscI AseI AsiSI BaeI BamHI BbeI BbvCI BcgI BfrBI BglI BlpI BmgBI BmrI BmtI BplI Bpu10I BsaAI BsaHI BsgI BsiEI BsiWI BsmI BspHI BssHII BssSI BstBI BstZ17I ClaI DraI DraIII DrdI EagI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI MluI NarI NheI NotI NruI NsiI PacI PmeI PmlI PpiI PsiI PspXI PsrI PstI PvuI SacII SalI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TstI Tth111I XbaI XhoI XmaI XmnI ZraI