################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI AhdI AlwNI Aor51HI AscI Asp700I AspEI AspI AvrII BalI BbeI BbrPI BciVI BclI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstZ17I CelII Cfr10I ClaI DraI DraIII EagI Eam1105I EarI EclXI Eco47III Eco52I EcoNI EcoT22I FauI FbaI FseI HgaI Hin1I HincII HindII HindIII HinfI HpaI KasI Ksp632I KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI PvuII RcaI SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI TfiI Tth111I XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 14 GGTACC G^GTACC AccI 482 GTAGAC GT^[AC][GT]AC Alw44I 168 GTGCAC G^TGCAC AocI 162 CCTCAGG CC^T[ACGT]AGG ApaI 330 GGGCCC GGGCC^C ApaLI 168 GTGCAC G^TGCAC AseI 439 ATTAAT AT^TAAT AsnI 439 ATTAAT AT^TAAT Asp718I 14 GGTACC G^GTACC AviII 92 TGCGCA TGC^GCA BamHI 252 GGATCC G^GATCC BanII 330 GGGCCC G[GA]GC[CT]^C BbsI 544 GAAGAC GAAGAC(2/6) BcgI 597 CGAGTGCAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfaI 377 CTAG C^TAG BpuAI 544 GAAGAC GAAGAC(2/6) BssSI 40 CTCGTG CACGAG(-5/-1) Bsu36I 162 CCTCAGG CC^T[ACGT]AGG Cac8I 916 GCCAGC GC[ACGT]^[ACGT]GC CpoI 137 CGGTCCG CG^G[AT]CCG DrdI 178 GACAACCGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 620 TGGCCG [CT]^GGCC[GA] Eco81I 162 CCTCAGG CC^T[ACGT]AGG EcoRI 754 GAATTC G^AATTC EcoRV 430 GATATC GAT^ATC EcoT14I 683 CCAAGG C^C[AT][AT]GG FspI 92 TGCGCA TGC^GCA HaeII 154 AGCGCC [GA]GCGC^[CT] KpnI 14 GGTACC GGTAC^C MaeI 377 CTAG C^TAG MseI 440 TTAA T^TAA MspA1I 96 CAGCGG C[AC]G^C[GT]G MwoI 461 GCCTCACCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC PflMI 306 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshBI 439 ATTAAT AT^TAAT PstI 721 CTGCAG CTGCA^G RsrII 137 CGGTCCG CG^G[AT]CCG SfcI 721 CTGCAG C^T[GA][CT]AG SspI 454 AATATT AAT^ATT StyI 683 CCAAGG C^C[AT][AT]GG TaqI 299 TCGA T^CGA Tru9I 440 TTAA T^TAA TthHB8I 299 TCGA T^CGA Van91I 306 CCAGAACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccII 259 CGCG CG^CG 624 CGCG AcsI 560 AAATTC [GA]^AATT[CT] 754 GAATTC AluI 120 AGCT AG^CT 899 AGCT ApoI 560 AAATTC [GA]^AATT[CT] 754 GAATTC AspHI 168 GTGCAC G[AT]GC[AT]^C 943 GTGCTC AvaI 191 CCCGGG C^[CT]CG[GA]G 575 CCCGGG BanI 14 GGTACC G^G[CT][GA]CC 245 GGTGCC BbvCI 734 CCTCAGC CCTCAGC(-5/-2) 746 CCTCAGC BsgI 349 GTGCAG GTGCAG(16/14) 600 GTGCAG BsiHKAI 168 GTGCAC G[AT]GC[AT]^C 943 GTGCTC BsoBI 191 CCCGGG C^[CT]CG[GA]G 575 CCCGGG BspMI 340 ACCTGC ACCTGC(4/8) 466 ACCTGC BsrI 478 ACTGG ACTGG(1/-1) 759 CCAGT BstEII 186 GGTCACC G^GT[ACGT]ACC 648 GGTCACC BstPI 186 GGTCACC G^GT[ACGT]ACC 648 GGTCACC BstUI 259 CGCG CG^CG 624 CGCG BstYI 252 GGATCC [GA]^GATC[CT] 302 AGATCC DpnII 253 GATC ^GATC 303 GATC DraII 329 AGGGCCC [GA]G^G[ACGT]CC[CT] 330 GGGCCCT DsaI 629 CCGTGG C^C[GA][CT]GG 701 CCGTGG EcoO109I 329 AGGGCCC [GA]G^G[ACGT]CC[CT] 330 GGGCCCT EcoO65I 186 GGTCACC G^GT[ACGT]ACC 648 GGTCACC MboI 253 GATC ^GATC 303 GATC MflI 252 GGATCC [GA]^GATC[CT] 302 AGATCC MvnI 259 CGCG CG^CG 624 CGCG NdeII 253 GATC ^GATC 303 GATC NlaIII 862 CATG CATG^ 924 CATG Sau3AI 253 GATC ^GATC 303 GATC SmaI 191 CCCGGG CCC^GGG 575 CCCGGG XhoII 252 GGATCC [GA]^GATC[CT] 302 AGATCC XmaCI 191 CCCGGG C^CCGGG 575 CCCGGG XmaI 191 CCCGGG C^CCGGG 575 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 47 enzymes) # ######################################### AciI AfaI AlwI AvaII BbvI BcnI BmyI BsaJI BsiYI BslI BsmAI BsmFI Bsp1286I BssKI BstF5I BstNI BstXI CfoI Cfr13I DdeI EcoRII Fnu4HI FokI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MaeII MaeIII MboII MnlI MslI MspI MvaI NciI NlaIV RsaI Sau96I ScrFI SfaNI TseI Tsp45I Tsp509I TspRI