(Circular) (Six-base) MAPSORT of: pFN21AE6631.seq Check: 2712 from: 1 to: 5653 pFN21AE6631 5653 bp With 182 enzymes: SgfI * March 18, 2015 14:53 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2354 2480 2354 Size: 126 5527 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3463 278 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2488 2996 1937 Size: 551 508 4594 Fragments arranged by size: 4594 551 508 Acc65I G'GTAC_C Cuts at: 2019 2974 2019 Size: 955 4698 AclI AA'CG_TT Cuts at: 1563 3780 4153 1563 Size: 2217 373 3063 Fragments arranged by size: 3063 2217 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2415 3716 5100 5368 1632 Size: 783 1301 1384 268 1917 Fragments arranged by size: 1917 1384 1301 783 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5456 AflIII A'CryG_T Cuts at: 1203 4552 5529 1203 Size: 3349 977 1327 Fragments arranged by size: 3349 1327 977 AgeI A'CCGG_T Cuts at: 4484 4484 Size: 5653 AhdI GACnn_n'nnGTC Cuts at: 4382 4382 Size: 5653 AleI CACnn'nnGTG Cuts at: 1166 2087 2198 2728 2888 1166 Size: 921 111 530 160 3931 Fragments arranged by size: 3931 921 530 160 111 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5653 AlwNI CAG_nnn'CTG Cuts at: 3396 4968 3396 Size: 1572 4081 ApaI G_GGCC'C Cuts at: 2339 2339 Size: 5653 ApaLI G'TGCA_C Cuts at: 2173 3710 4866 2173 Size: 1537 1156 2960 Fragments arranged by size: 2960 1537 1156 ApoI r'AATT_y Cuts at: 1493 2565 2759 2962 3188 3340 5449 1493 Size: 1072 194 203 226 152 2109 1697 Fragments arranged by size: 2109 1697 1072 226 203 194 152 AseI AT'TA_AT Cuts at: 160 1032 2445 4207 160 Size: 872 1413 1762 1606 Fragments arranged by size: 1762 1606 1413 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 AvaI C'yCGr_G Cuts at: 1870 1943 2196 2580 2978 1870 Size: 73 253 384 398 4545 Fragments arranged by size: 4545 398 384 253 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2685 2718 2685 Size: 33 5620 BamHI G'GATC_C Cuts at: 2257 2983 3350 2257 Size: 726 367 4560 Fragments arranged by size: 4560 726 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2019 2250 2974 4429 618 Size: 325 217 627 232 231 724 1455 1842 Fragments arranged by size: 1842 1455 724 627 325 232 231 217 BanII G_rGCy'C Cuts at: 1391 2339 2972 1391 Size: 948 633 4072 Fragments arranged by size: 4072 948 633 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2556 928 Size: 971 657 4025 Fragments arranged by size: 4025 971 657 BbvCI CC'TCA_GC Cuts at: 2740 2752 2740 Size: 12 5641 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2591 2625 3844 3878 1903 Size: 34 654 34 1219 34 3678 Fragments arranged by size: 3678 1219 654 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3545 4755 3545 Size: 1210 4443 BclI T'GATC_A Cuts at: 1548 1548 Size: 5653 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4264 136 Size: 107 122 71 631 605 2592 1525 Fragments arranged by size: 2592 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5648 5648 Size: 5653 BlpI GC'TnA_GC Cuts at: 3070 3070 Size: 5653 Bme1580I G_kGCm'C Cuts at: 1790 2094 2177 2339 3714 4870 1790 Size: 304 83 162 1375 1156 2573 Fragments arranged by size: 2573 1375 1156 304 162 83 BmgBI CAC'GTC Cuts at: 1318 2623 1318 Size: 1305 4348 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3384 4342 442 Size: 416 53 83 399 24 1967 958 1753 Fragments arranged by size: 1967 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5653 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4313 1239 Size: 3074 2579 Bpu10I CC'TnA_GC Cuts at: 2740 2752 2740 Size: 12 5641 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3778 4643 4941 3778 Size: 865 298 4490 Fragments arranged by size: 4490 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4316 882 Size: 617 2817 2219 Fragments arranged by size: 2817 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3444 493 Size: 711 2240 2702 Fragments arranged by size: 2702 2240 711 BsaBI GATnn'nnATC Cuts at: 5647 5647 Size: 5653 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3460 3842 4477 275 Size: 53 83 186 936 1927 382 635 1451 Fragments arranged by size: 1927 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4086 4484 4758 4905 5316 4086 Size: 398 274 147 411 4423 Fragments arranged by size: 4423 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2079 2109 1178 Size: 30 165 30 676 30 4722 Fragments arranged by size: 4722 676 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5653 BseYI C'CCAG_C Cuts at: 2234 2905 4856 5352 2234 Size: 671 1951 496 2535 Fragments arranged by size: 2535 1951 671 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 2626 1163 Size: 573 639 251 4190 Fragments arranged by size: 4190 639 573 251 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3103 3864 4892 1540 Size: 275 186 1102 761 1028 2301 Fragments arranged by size: 2301 1102 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2177 2952 3714 3799 4870 2177 Size: 775 762 85 1071 2960 Fragments arranged by size: 2960 1071 775 762 85 BsmI GAATG_Cn' Cuts at: 3164 3257 3164 Size: 93 5560 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2094 2177 2339 2952 2972 3714 3799 Size: 399 304 83 162 613 20 742 85 Cuts at: 3799 4870 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 613 399 304 162 85 83 20 BspEI T'CCGG_A Cuts at: 5316 5316 Size: 5653 BspHI T'CATG_A Cuts at: 3542 5272 3542 Size: 1730 3923 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2354 2480 3008 844 Size: 1510 126 528 3489 Fragments arranged by size: 3489 1510 528 126 BsrBI CCG'CTC Cuts at: 1394 1823 3540 1394 Size: 429 1717 3507 Fragments arranged by size: 3507 1717 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4148 4322 65 Size: 1183 2900 174 1396 Fragments arranged by size: 2900 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4297 4484 1700 Size: 110 2487 187 2869 Fragments arranged by size: 2869 2487 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5653 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5653 BssSI C'ACGA_G Cuts at: 2045 3713 4725 2045 Size: 1668 1012 2973 Fragments arranged by size: 2973 1668 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5653 BstBI TT'CG_AA Cuts at: 4457 4457 Size: 5653 BstEII G'GTnAC_C Cuts at: 1184 2191 2653 1184 Size: 1007 462 4184 Fragments arranged by size: 4184 1007 462 BstXI CCAn_nnnn'nTGG Cuts at: 2128 2623 2905 2128 Size: 495 282 4876 Fragments arranged by size: 4876 495 282 BstYI r'GATC_y Cuts at: 1972 2257 2307 2983 3350 3735 3752 5204 5290 Size: 285 50 676 367 385 17 1452 86 Cuts at: 5290 5302 5648 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 676 385 367 346 285 86 50 17 12 Bsu36I CC'TnA_GG Cuts at: 2168 2168 Size: 5653 BtgI C'CryG_G Cuts at: 513 1065 2004 2634 2706 513 Size: 552 939 630 72 3460 Fragments arranged by size: 3460 939 630 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4914 Fragments arranged by size: 4914 683 56 BtsI GCAGTG_nn' Cuts at: 2614 2723 2735 3169 3963 3983 2614 Size: 109 12 434 794 20 4284 Fragments arranged by size: 4284 794 434 109 20 12 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5653 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5618 DraI TTT'AAA Cuts at: 2957 2957 Size: 5653 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2189 4660 809 Size: 507 873 2471 1802 Fragments arranged by size: 2471 1802 873 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2625 3100 3989 Size: 54 1425 50 275 18 795 475 889 Cuts at: 3989 5618 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 795 475 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3100 1537 Size: 275 1288 4090 Fragments arranged by size: 4090 1288 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3583 1353 Size: 2230 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4240 4614 4760 387 Size: 1064 138 2651 374 146 1280 Fragments arranged by size: 2651 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2415 3716 4313 5100 5368 1239 Size: 393 783 1301 597 787 268 1524 Fragments arranged by size: 1524 1301 787 783 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2335 2336 2335 Size: 1 5652 EcoRI G'AATT_C Cuts at: 2759 2962 3340 2759 Size: 203 378 5072 Fragments arranged by size: 5072 378 203 EcoRV GAT'ATC Cuts at: 2437 2437 Size: 5653 FspI TGC'GCA Cuts at: 2099 4159 2099 Size: 2060 3593 HaeII r_GCGC'y Cuts at: 1678 2163 4800 1678 Size: 485 2637 2531 Fragments arranged by size: 2637 2531 485 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2637 2669 2817 2849 4294 Size: 32 135 32 1065 32 148 32 1445 Cuts at: 4294 4326 4368 4400 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1445 1065 148 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2997 3243 669 Size: 988 281 1059 246 3079 Fragments arranged by size: 3079 1059 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5653 HpaI GTT'AAC Cuts at: 3243 3243 Size: 5653 KpnI G_GTAC'C Cuts at: 2023 2978 2023 Size: 955 4698 MfeI C'AATT_G Cuts at: 3252 3252 Size: 5653 MluI A'CGCG_T Cuts at: 5529 5529 Size: 5653 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2922 4767 4951 1305 Size: 1617 1845 184 2007 Fragments arranged by size: 2007 1845 1617 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3831 Fragments arranged by size: 3831 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2087 2198 2728 2871 2888 2916 3611 Size: 648 921 111 530 143 17 28 695 Cuts at: 3611 3970 4129 518 Size: 359 159 2042 Fragments arranged by size: 2042 921 695 648 530 359 159 143 111 28 17 MspA1I CmG'CkG Cuts at: 1606 2103 3069 3746 4894 5139 1606 Size: 497 966 677 1148 245 2120 Fragments arranged by size: 2120 1148 966 677 497 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4162 Fragments arranged by size: 4162 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5653 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5653 NotI GC'GGCC_GC Cuts at: 3100 3100 Size: 5653 NspI r_CATG'y Cuts at: 1132 1229 3011 4556 1132 Size: 97 1782 1545 2229 Fragments arranged by size: 2229 1782 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5653 PciI A'CATG_T Cuts at: 4552 4552 Size: 5653 PflMI CCAn_nnn'nTGG Cuts at: 1108 2317 1108 Size: 1209 4444 PmeI GTTT'AAAC Cuts at: 2957 2957 Size: 5653 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3722 3754 5235 5267 3722 Size: 32 1481 32 4108 Fragments arranged by size: 4108 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5653 PsiI TTA'TAA Cuts at: 3223 3223 Size: 5653 PspOMI G'GGCC_C Cuts at: 2335 2335 Size: 5653 PstI C_TGCA'G Cuts at: 830 2730 3005 830 Size: 1900 275 3478 Fragments arranged by size: 3478 1900 275 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5653 RsrII CG'GwC_CG Cuts at: 2143 2143 Size: 5653 SalI G'TCGA_C Cuts at: 1936 2995 1936 Size: 1059 4594 SbfI CC_TGCA'GG Cuts at: 3005 3005 Size: 5653 ScaI AGT'ACT Cuts at: 3901 5613 3901 Size: 1712 3941 SfcI C'TryA_G Cuts at: 826 1045 2726 3001 4136 4817 5008 826 Size: 219 1681 275 1135 681 191 1471 Fragments arranged by size: 1681 1471 1135 681 275 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5653 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5653 SmaI CCC'GGG Cuts at: 1872 2198 2582 2980 1872 Size: 326 384 398 4545 Fragments arranged by size: 4545 398 384 326 SmlI C'TyrA_G Cuts at: 820 1017 1943 3757 4658 4920 820 Size: 197 926 1814 901 262 1553 Fragments arranged by size: 1814 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3444 493 Size: 2951 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5653 SphI G_CATG'C Cuts at: 1132 3011 1132 Size: 1879 3774 SspI AAT'ATT Cuts at: 5 52 2461 3577 5 Size: 47 2409 1116 2081 Fragments arranged by size: 2409 2081 1116 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5653 StyI C'CwwG_G Cuts at: 513 1065 2004 2688 3092 513 Size: 552 939 684 404 3074 Fragments arranged by size: 3074 939 684 552 404 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2300 2385 3680 3697 3850 4035 4480 Size: 1255 720 85 1295 17 153 185 445 Cuts at: 4480 325 Size: 1498 Fragments arranged by size: 1498 1295 1255 720 445 185 153 85 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2295 3899 5611 Size: 274 80 33 51 1443 318 1604 1712 Cuts at: 5611 96 Size: 138 Fragments arranged by size: 1712 1604 1443 318 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2079 2111 2588 2620 5613 5645 2079 Size: 32 477 32 2993 32 2087 Fragments arranged by size: 2993 2087 477 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5653 XbaI T'CTAG_A Cuts at: 2989 2989 Size: 5653 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5653 XmaI C'CCGG_G Cuts at: 1870 2196 2580 2978 1870 Size: 326 384 398 4545 Fragments arranged by size: 4545 398 384 326 XmnI GAAnn'nnTTC Cuts at: 3782 3782 Size: 5653 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3461 276 Size: 53 83 186 936 1927 2468 Fragments arranged by size: 2468 1927 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI PvuI RsrII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BbeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspXI PsrI PvuII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI