################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AatII AccI AclI AcsI AflII AgeI Aor51HI ApoI AseI AsnI AspI AvrII BbsI BcgI BciVI BclI BfrI BglII BlnI BpuAI BsaBI BsiWI BsmI Bsp1407I BspDI BspHI BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI Eco47III EcoRI EcoRV FbaI FseI HincII HindII HindIII HpaI MamI MfeI MluI MunI NcoI NdeI NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SfuI SnaBI SpeI SphI SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Acc65I 1006 GGTACC G^GTACC AhdI 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2838 CCTGAGG CC^T[ACGT]AGG AscI 623 GGCGCGCC GG^CGCGCC Asp700I 1998 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1006 GGTACC G^GTACC AspEI 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 866 TGCGCA TGC^GCA BamHI 1566 GGATCC G^GATCC BbrPI 1093 CACGTG CAC^GTG BbvCI 2475 CCTCAGC CCTCAGC(-5/-2) BfaI 425 CTAG C^TAG BglI 2765 GCCACGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaI 503 GAGACC GGTCTC(1/5) BsmBI 661 GAGACG CGTCTC(1/5) BspLU11I 1591 ACATGT A^CATGT BsrDI 848 GCAATG GCAATG(2/0) BssSI 1081 CACGAG CACGAG(-5/-1) Bsu36I 2838 CCTGAGG CC^T[ACGT]AGG DrdI 2827 GACTTCTGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 2450 GACCCTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2838 CCTGAGG CC^T[ACGT]AGG EcoNI 1296 CCTGCCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 2077 ATGCAT ATGCA^T FspI 866 TGCGCA TGC^GCA KpnI 1006 GGTACC GGTAC^C MaeI 425 CTAG C^TAG MseI 104 TTAA T^TAA NheI 424 GCTAGC G^CTAGC NsiI 2077 ATGCAT ATGCA^T PmaCI 1093 CACGTG CAC^GTG PmlI 1093 CACGTG CAC^GTG SacI 2692 GAGCTC GAGCT^C SapI 281 GCTCTTC GCTCTTC(1/4) SbfI 2304 CCTGCAGG CCTGCA^GG SfiI 2764 GGCCACGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SgrAI 431 CGCCGGCG C[GA]^CCGG[CT]G Sse8387I 2304 CCTGCAGG CCTGCA^GG TfiI 2003 GATTC G^A[AT]TC Tru9I 104 TTAA T^TAA Tsp509I 2089 AATT ^AATT XmnI 1998 GAAGAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccIII 499 TCCGGA T^CCGGA 1723 TCCGGA AflIII 1094 ACGTGT A^C[GA][CT]GT 1591 ACATGT Alw44I 1407 GTGCAC G^TGCAC 1626 GTGCAC ApaI 557 GGGCCC GGGCC^C 832 GGGCCC ApaLI 1407 GTGCAC G^TGCAC 1626 GTGCAC BalI 1613 TGGCCA TGG^CCA 2099 TGGCCA BlpI 1715 GCTCAGC GC^T[ACGT]AGC 2694 GCTCAGC Bpu1102I 1715 GCTCAGC GC^T[ACGT]AGC 2694 GCTCAGC BsaAI 655 TACGTG [CT]AC^GT[GA] 1093 CACGTG BsaWI 499 TCCGGA [AT]^CCGG[AT] 1723 TCCGGA BseAI 499 TCCGGA T^CCGGA 1723 TCCGGA BseRI 1132 GAGGAG GAGGAG(10/8) 1741 GAGGAG BspEI 499 TCCGGA T^CCGGA 1723 TCCGGA BsrBI 64 GAGCGG CCGCTC(-3/-3) 2239 GAGCGG BssHII 428 GCGCGC G^CGCGC 624 GCGCGC BstEII 840 GGTCACC G^GT[ACGT]ACC 2705 GGTCACC BstPI 840 GGTCACC G^GT[ACGT]ACC 2705 GGTCACC BstYI 1305 GGATCT [GA]^GATC[CT] 1566 GGATCC CelII 1715 GCTCAGC GC^T[ACGT]AGC 2694 GCTCAGC DraIII 1537 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1914 CACGCTGTG EagI 108 CGGCCG C^GGCCG 2727 CGGCCG EclXI 108 CGGCCG C^GGCCG 2727 CGGCCG Eco52I 108 CGGCCG C^GGCCG 2727 CGGCCG EcoO65I 840 GGTCACC G^GT[ACGT]ACC 2705 GGTCACC MflI 1305 GGATCT [GA]^GATC[CT] 1566 GGATCC MluNI 1613 TGGCCA TGG^CCA 2099 TGGCCA MroI 499 TCCGGA T^CCGGA 1723 TCCGGA MscI 1613 TGGCCA TGG^CCA 2099 TGGCCA SexAI 2246 ACCTGGT A^CC[AT]GGT 2489 ACCAGGT SmlI 2118 CTCAAG C^T[CT][GA]AG 2752 CTCAAG XcmI 239 CCACTGAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1421 CCAAGGGACAGATGG XhoII 1305 GGATCT [GA]^GATC[CT] 1566 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AcyI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanI BanII BbeI BbvI BcnI BmyI BpmI BsaHI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I KspI MaeII MaeIII MboI MboII MnlI MslI MspA1I MspI MvaI MvnI MwoI NaeI NarI NciI NdeII NgoMIV NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacII Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SfoI SmaI StyI TaqI TseI Tsp45I TspRI TthHB8I Van91I XmaCI XmaI