(Circular) (Six-base) MAPSORT of: pFN21AE6764.seq Check: 7608 from: 1 to: 7555 pFN21AE6764 7555 bp With 182 enzymes: SgfI * March 18, 2015 14:54 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5365 278 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4898 1937 Size: 2961 4594 Acc65I G'GTAC_C Cuts at: 3011 4876 3011 Size: 1865 5690 AclI AA'CG_TT Cuts at: 1563 5682 6055 1563 Size: 4119 373 3063 Fragments arranged by size: 4119 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2274 4208 5618 7002 7270 1632 Size: 642 1934 1410 1384 268 1917 Fragments arranged by size: 1934 1917 1410 1384 642 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7358 AflIII A'CryG_T Cuts at: 1203 3099 3596 6454 7431 1203 Size: 1896 497 2858 977 1327 Fragments arranged by size: 2858 1896 1327 977 497 AgeI A'CCGG_T Cuts at: 6386 6386 Size: 7555 AhdI GACnn_n'nnGTC Cuts at: 4460 6284 4460 Size: 1824 5731 AleI CACnn'nnGTG Cuts at: 1166 4298 4759 1166 Size: 3132 461 3962 Fragments arranged by size: 3962 3132 461 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4226 4289 4595 1184 Size: 3042 63 306 4144 Fragments arranged by size: 4144 3042 306 63 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2207 2239 3980 4012 2207 Size: 32 1741 32 5750 Fragments arranged by size: 5750 1741 32 32 AlwNI CAG_nnn'CTG Cuts at: 2965 4707 4828 5298 6870 2965 Size: 1742 121 470 1572 3650 Fragments arranged by size: 3650 1742 1572 470 121 ApaI G_GGCC'C Cuts at: 2566 2841 2566 Size: 275 7280 ApaLI G'TGCA_C Cuts at: 3412 3631 5612 6768 3412 Size: 219 1981 1156 4199 Fragments arranged by size: 4199 1981 1156 219 ApoI r'AATT_y Cuts at: 1493 4864 5090 5242 7351 1493 Size: 3371 226 152 2109 1697 Fragments arranged by size: 3371 2109 1697 226 152 AscI GG'CGCG_CC Cuts at: 2629 2629 Size: 7555 AseI AT'TA_AT Cuts at: 160 1032 6109 160 Size: 872 5077 1606 Fragments arranged by size: 5077 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 AvaI C'yCGr_G Cuts at: 1870 1943 2138 2536 2924 3305 3840 4380 4416 Size: 73 195 398 388 381 535 540 36 Cuts at: 4416 4449 4791 4880 1870 Size: 33 342 89 4545 Fragments arranged by size: 4545 540 535 398 388 381 342 195 89 73 36 33 BamHI G'GATC_C Cuts at: 3571 4885 5252 3571 Size: 1314 367 5874 Fragments arranged by size: 5874 1314 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2396 3011 3320 3473 3814 Size: 325 217 627 609 615 309 153 341 Cuts at: 3814 3898 4118 4876 6331 618 Size: 84 220 758 1455 1842 Fragments arranged by size: 1842 1455 758 627 615 609 341 325 309 220 217 153 84 BanII G_rGCy'C Cuts at: 1391 2277 2288 2373 2566 2841 3371 3711 3998 Size: 886 11 85 193 275 530 340 287 Cuts at: 3998 4610 4683 4701 4874 1391 Size: 612 73 18 173 4072 Fragments arranged by size: 4072 886 612 530 340 287 275 193 173 85 73 18 11 BbeI G_GCGC'C Cuts at: 2400 3324 4122 2400 Size: 924 798 5833 Fragments arranged by size: 5833 924 798 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6584 BbvCI CC'TCA_GC Cuts at: 4481 4481 Size: 7555 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 5746 5780 1903 Size: 34 50 34 3725 34 3678 Fragments arranged by size: 3725 3678 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5447 6657 5447 Size: 1210 6345 BclI T'GATC_A Cuts at: 1548 1548 Size: 7555 BfrBI ATG'CAT Cuts at: 4084 4084 Size: 7555 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4776 6166 136 Size: 107 122 71 631 605 3104 1390 1525 Fragments arranged by size: 3104 1525 1390 631 605 122 107 71 BglII A'GATC_T Cuts at: 7550 7550 Size: 7555 BlpI GC'TnA_GC Cuts at: 3721 4700 4972 3721 Size: 979 272 6304 Fragments arranged by size: 6304 979 272 Bme1580I G_kGCm'C Cuts at: 1790 2566 2841 3261 3416 3635 3819 4295 5616 Size: 776 275 420 155 219 184 476 1321 Cuts at: 5616 6772 1790 Size: 1156 2573 Fragments arranged by size: 2573 1321 1156 776 476 420 275 219 184 155 BmgBI CAC'GTC Cuts at: 1318 2323 1318 Size: 1005 6550 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2941 5286 6244 Size: 416 53 83 399 24 1524 2345 958 Cuts at: 6244 442 Size: 1753 Fragments arranged by size: 2345 1753 1524 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2433 1056 Size: 1377 6178 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2356 4372 2356 Size: 2016 5539 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2073 2252 2459 3725 4352 6215 1239 Size: 834 179 207 1266 627 1863 2579 Fragments arranged by size: 2579 1863 1266 834 627 207 179 Bpu10I CC'TnA_GC Cuts at: 3335 4481 3335 Size: 1146 6409 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4108 4742 5680 6545 6843 4108 Size: 634 938 865 298 4820 Fragments arranged by size: 4820 938 865 634 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2502 6218 882 Size: 617 1003 3716 2219 Fragments arranged by size: 3716 2219 1003 617 BsaAI yAC'GTr Cuts at: 493 1204 2662 3100 5346 493 Size: 711 1458 438 2246 2702 Fragments arranged by size: 2702 2246 1458 711 438 BsaBI GATnn'nnATC Cuts at: 7549 7549 Size: 7555 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2210 2259 2397 3321 Size: 53 83 186 936 677 49 138 924 Cuts at: 3321 4119 5362 5744 6379 275 Size: 798 1243 382 635 1451 Fragments arranged by size: 1451 1243 936 924 798 677 635 382 186 138 83 53 49 BsaWI w'CCGG_w Cuts at: 2504 3728 5988 6386 6660 6807 7218 2504 Size: 1224 2260 398 274 147 411 2841 Fragments arranged by size: 2841 2260 1224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2457 2487 3666 3696 3737 Size: 30 165 30 1054 30 1179 30 41 Cuts at: 3737 3767 3926 3956 3980 4010 4412 4442 1178 Size: 30 159 30 24 30 402 30 4291 Fragments arranged by size: 4291 1179 1054 402 165 159 41 30 30 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3152 3761 1186 Size: 1966 609 4980 Fragments arranged by size: 4980 1966 609 BseYI C'CCAG_C Cuts at: 2014 2771 3091 3284 3716 4530 4545 6758 7254 Size: 757 320 193 432 814 15 2213 496 Cuts at: 7254 2014 Size: 2315 Fragments arranged by size: 2315 2213 814 757 496 432 320 193 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3382 3632 4509 1163 Size: 573 1646 250 877 4209 Fragments arranged by size: 4209 1646 877 573 250 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2116 3199 4735 5005 5766 6794 Size: 275 186 115 1083 1536 270 761 1028 Cuts at: 6794 1540 Size: 2301 Fragments arranged by size: 2301 1536 1083 1028 761 275 270 186 115 BsiHKAI G_wGCw'C Cuts at: 3416 3635 3929 4559 4701 5616 5701 6772 3416 Size: 219 294 630 142 915 85 1071 4199 Fragments arranged by size: 4199 1071 915 630 294 219 142 85 BsmI GAATG_Cn' Cuts at: 5066 5159 5066 Size: 93 7462 BsmBI CGTCTCn'nnnn_ Cuts at: 2660 2660 Size: 7555 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2277 2288 2373 2566 2841 3261 3371 Size: 399 487 11 85 193 275 420 110 Cuts at: 3371 3416 3635 3711 3819 3929 3998 4295 4559 Size: 45 219 76 108 110 69 297 264 Cuts at: 4559 4610 4683 4701 4874 5616 5701 6772 1391 Size: 51 73 18 173 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 487 420 399 297 275 264 219 193 173 110 110 108 85 85 76 73 69 51 45 18 11 BspEI T'CCGG_A Cuts at: 2504 3728 7218 2504 Size: 1224 3490 2841 Fragments arranged by size: 3490 2841 1224 BspHI T'CATG_A Cuts at: 5444 7174 5444 Size: 1730 5825 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2304 2610 4218 4910 844 Size: 1460 306 1608 692 3489 Fragments arranged by size: 3489 1608 1460 692 306 BsrBI CCG'CTC Cuts at: 1394 1823 2071 4246 5442 1394 Size: 429 248 2175 1196 3507 Fragments arranged by size: 3507 2175 1196 429 248 BsrDI GCAATG_nn' Cuts at: 65 1248 2860 6050 6224 65 Size: 1183 1612 3190 174 1396 Fragments arranged by size: 3190 1612 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2437 2613 2849 3789 4779 6199 6386 Size: 110 627 176 236 940 990 1420 187 Cuts at: 6386 1700 Size: 2869 Fragments arranged by size: 2869 1420 990 940 627 236 187 176 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7555 BssHII G'CGCG_C Cuts at: 1925 2433 2629 1925 Size: 508 196 6851 Fragments arranged by size: 6851 508 196 BssSI C'ACGA_G Cuts at: 3086 5615 6627 3086 Size: 2529 1012 4014 Fragments arranged by size: 4014 2529 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3498 3666 3924 794 Size: 2704 168 258 4425 Fragments arranged by size: 4425 2704 258 168 BstBI TT'CG_AA Cuts at: 6359 6359 Size: 7555 BstEII G'GTnAC_C Cuts at: 1184 2845 4710 1184 Size: 1661 1865 4029 Fragments arranged by size: 4029 1865 1661 BstXI CCAn_nnnn'nTGG Cuts at: 2095 3910 4498 2095 Size: 1815 588 5152 Fragments arranged by size: 5152 1815 588 BstYI r'GATC_y Cuts at: 1972 3310 3571 4885 5252 5637 5654 7106 7192 Size: 1338 261 1314 367 385 17 1452 86 Cuts at: 7192 7204 7550 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1338 1314 385 367 346 261 86 17 12 Bsu36I CC'TnA_GG Cuts at: 4844 4844 Size: 7555 BtgI C'CryG_G Cuts at: 513 1065 2374 2582 2654 2704 3804 4344 4359 Size: 552 1309 208 72 50 1100 540 15 Cuts at: 4359 4656 4771 513 Size: 297 115 3297 Fragments arranged by size: 3297 1309 1100 552 540 297 208 115 72 50 15 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6816 Fragments arranged by size: 6816 683 56 BtsI GCAGTG_nn' Cuts at: 2937 3078 3229 3350 3635 3758 4579 5071 5865 Size: 141 151 121 285 123 821 492 794 Cuts at: 5865 5885 2937 Size: 20 4607 Fragments arranged by size: 4607 821 794 492 285 151 141 123 121 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7555 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7520 DraI TTT'AAA Cuts at: 4859 4859 Size: 7555 DraIII CAC_nnn'GTG Cuts at: 3547 3924 3547 Size: 377 7178 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4838 6562 809 Size: 507 3522 1724 1802 Fragments arranged by size: 3522 1802 1724 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2113 3618 4104 Size: 54 1425 50 275 18 283 1505 486 Cuts at: 4104 4732 5002 5891 7520 8 Size: 628 270 889 1629 43 Fragments arranged by size: 1629 1505 1425 889 628 486 283 275 270 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2113 4732 5002 1537 Size: 275 301 2619 270 4090 Fragments arranged by size: 4090 2619 301 275 270 EarI CTCTTCn'nnn_ Cuts at: 1353 2293 2954 3998 4073 5485 1353 Size: 940 661 1044 75 1412 3423 Fragments arranged by size: 3423 1412 1044 940 661 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4127 4600 6142 6516 6662 387 Size: 1064 138 2538 473 1542 374 146 1280 Fragments arranged by size: 2538 1542 1280 1064 473 374 146 138 EcoICRI GAG'CTC Cuts at: 4699 4699 Size: 7555 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2073 2252 2274 2459 3725 4208 4352 Size: 393 441 179 22 185 1266 483 144 Cuts at: 4352 5618 6215 7002 7270 1239 Size: 1266 597 787 268 1524 Fragments arranged by size: 1524 1266 1266 787 597 483 441 393 268 185 179 144 22 EcoNI CCTnn'n_nnAGG Cuts at: 3305 3305 Size: 7555 EcoO109I rG'GnC_Cy Cuts at: 2135 2563 2962 3510 4249 4351 4444 4454 4518 Size: 428 399 548 739 102 93 10 64 Cuts at: 4518 2135 Size: 5172 Fragments arranged by size: 5172 739 548 428 399 102 93 64 10 EcoRI G'AATT_C Cuts at: 4864 5242 4864 Size: 378 7177 FspI TGC'GCA Cuts at: 2873 6061 2873 Size: 3188 4367 HaeII r_GCGC'y Cuts at: 1678 2400 2482 3324 4122 4707 4753 6702 1678 Size: 722 82 842 798 585 46 1949 2531 Fragments arranged by size: 2531 1949 842 798 722 585 82 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2501 2533 2816 2848 3516 Size: 32 135 32 929 32 283 32 668 Cuts at: 3516 3548 3926 3958 4412 4415 4444 4446 4447 Size: 32 378 32 454 3 29 2 1 Cuts at: 4447 4478 6196 6228 6270 6302 1373 Size: 31 1718 32 42 32 2626 Fragments arranged by size: 2626 1718 929 668 454 378 283 135 42 32 32 32 32 32 32 32 32 31 29 3 2 1 HincII GTy'rAC Cuts at: 669 1657 1938 4899 5145 669 Size: 988 281 2961 246 3079 Fragments arranged by size: 3079 2961 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7555 HpaI GTT'AAC Cuts at: 5145 5145 Size: 7555 KasI G'GCGC_C Cuts at: 2396 3320 4118 2396 Size: 924 798 5833 Fragments arranged by size: 5833 924 798 KpnI G_GTAC'C Cuts at: 3015 4880 3015 Size: 1865 5690 MfeI C'AATT_G Cuts at: 5154 5154 Size: 7555 MluI A'CGCG_T Cuts at: 7431 7431 Size: 7555 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2116 4515 6669 6853 1305 Size: 811 2399 2154 184 2007 Fragments arranged by size: 2399 2154 2007 811 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3620 4106 10 Size: 54 1425 343 1788 486 3459 Fragments arranged by size: 3459 1788 1425 486 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2798 3120 4298 4759 5513 5872 6031 Size: 648 1632 322 1178 461 754 359 159 Cuts at: 6031 518 Size: 2042 Fragments arranged by size: 2042 1632 1178 754 648 461 359 322 159 MspA1I CmG'CkG Cuts at: 1606 2051 2376 2419 2584 2656 3255 3480 3616 Size: 445 325 43 165 72 599 225 136 Cuts at: 3616 4284 4298 4339 4545 4971 5648 6796 7041 Size: 668 14 41 206 426 677 1148 245 Cuts at: 7041 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 668 599 445 426 325 245 225 206 165 136 72 43 41 14 NaeI GCC'GGC Cuts at: 2439 2615 4781 2439 Size: 176 2166 5213 Fragments arranged by size: 5213 2166 176 NarI GG'CG_CC Cuts at: 2397 3321 4119 2397 Size: 924 798 5833 Fragments arranged by size: 5833 924 798 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 7003 NdeI CA'TA_TG Cuts at: 387 387 Size: 7555 NgoMIV G'CCGG_C Cuts at: 2437 2613 4779 2437 Size: 176 2166 5213 Fragments arranged by size: 5213 2166 176 NheI G'CTAG_C Cuts at: 1052 2429 1052 Size: 1377 6178 NotI GC'GGCC_GC Cuts at: 5002 5002 Size: 7555 NsiI A_TGCA'T Cuts at: 4086 4086 Size: 7555 NspI r_CATG'y Cuts at: 1132 1229 3112 3391 3600 4913 6458 1132 Size: 97 1883 279 209 1313 1545 2229 Fragments arranged by size: 2229 1883 1545 1313 279 209 97 PasI CC'CwG_GG Cuts at: 1348 2992 3462 1348 Size: 1644 470 5441 Fragments arranged by size: 5441 1644 470 PciI A'CATG_T Cuts at: 3596 6454 3596 Size: 2858 4697 PflMI CCAn_nnn'nTGG Cuts at: 1108 3091 3210 3408 4828 1108 Size: 1983 119 198 1420 3835 Fragments arranged by size: 3835 1983 1420 198 119 PfoI T'CCnGG_A Cuts at: 3246 3273 3741 4329 3246 Size: 27 468 588 6472 Fragments arranged by size: 6472 588 468 27 PmeI GTTT'AAAC Cuts at: 4859 4859 Size: 7555 PmlI CAC'GTG Cuts at: 3100 3100 Size: 7555 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5624 5656 7137 7169 3980 Size: 32 1612 32 1481 32 4366 Fragments arranged by size: 4366 1612 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2135 2962 4249 4351 4444 4454 2135 Size: 827 1287 102 93 10 5236 Fragments arranged by size: 5236 1287 827 102 93 10 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7555 PsiI TTA'TAA Cuts at: 5125 5125 Size: 7555 PspOMI G'GGCC_C Cuts at: 2562 2837 2562 Size: 275 7280 PstI C_TGCA'G Cuts at: 830 3224 4314 4584 4907 830 Size: 2394 1090 270 323 3478 Fragments arranged by size: 3478 2394 1090 323 270 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7555 PvuII CAG'CTG Cuts at: 3480 3616 4545 3480 Size: 136 929 6490 Fragments arranged by size: 6490 929 136 SacI G_AGCT'C Cuts at: 4701 4701 Size: 7555 SacII CC_GC'GG Cuts at: 2377 2585 2657 2377 Size: 208 72 7275 Fragments arranged by size: 7275 208 72 SalI G'TCGA_C Cuts at: 1936 4897 1936 Size: 2961 4594 SanDI GG'GwC_CC Cuts at: 2135 4454 2135 Size: 2319 5236 SapI GCTCTTCn'nnn_ Cuts at: 2293 2293 Size: 7555 SbfI CC_TGCA'GG Cuts at: 4314 4907 4314 Size: 593 6962 ScaI AGT'ACT Cuts at: 2930 3395 3665 3824 5803 7515 2930 Size: 465 270 159 1979 1712 2970 Fragments arranged by size: 2970 1979 1712 465 270 159 SexAI A'CCwGG_T Cuts at: 4251 4494 4251 Size: 243 7312 SfcI C'TryA_G Cuts at: 826 1045 3220 4310 4580 4903 6038 6719 6910 Size: 219 2175 1090 270 323 1135 681 191 Cuts at: 6910 826 Size: 1471 Fragments arranged by size: 2175 1471 1135 1090 681 323 270 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 4776 4776 Size: 7555 SfoI GGC'GCC Cuts at: 2398 3322 4120 2398 Size: 924 798 5833 Fragments arranged by size: 5833 924 798 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7555 SgrAI Cr'CCGG_yG Cuts at: 1700 2437 1700 Size: 737 6818 SmaI CCC'GGG Cuts at: 1872 3842 4382 4451 4793 4882 1872 Size: 1970 540 69 342 89 4545 Fragments arranged by size: 4545 1970 540 342 89 69 SmlI C'TyrA_G Cuts at: 820 1017 1943 4123 4757 5659 6560 6822 820 Size: 197 926 2180 634 902 901 262 1553 Fragments arranged by size: 2180 1553 926 902 901 634 262 197 SnaBI TAC'GTA Cuts at: 493 5346 493 Size: 4853 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7555 SphI G_CATG'C Cuts at: 1132 4913 1132 Size: 3781 3774 SspI AAT'ATT Cuts at: 5 52 5479 5 Size: 47 5427 2081 Fragments arranged by size: 5427 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7555 StyI C'CwwG_G Cuts at: 513 1065 2841 3426 3903 3955 4198 4440 4994 Size: 552 1776 585 477 52 243 242 554 Cuts at: 4994 513 Size: 3074 Fragments arranged by size: 3074 1776 585 554 552 477 243 242 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3802 3916 4522 5582 5599 5752 5937 Size: 1255 2222 114 606 1060 17 153 185 Cuts at: 5937 6382 325 Size: 445 1498 Fragments arranged by size: 2222 1498 1255 1060 606 445 185 153 114 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2928 3393 3663 Size: 274 80 33 51 1443 951 465 270 Cuts at: 3663 3822 5801 7513 96 Size: 159 1979 1712 138 Fragments arranged by size: 1979 1712 1443 951 465 274 270 159 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2457 2489 3158 3190 3423 3455 3666 3698 3737 Size: 32 669 32 233 32 211 32 39 Cuts at: 3737 3769 7515 7547 2457 Size: 32 3746 32 2465 Fragments arranged by size: 3746 2465 669 233 211 39 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7555 XbaI T'CTAG_A Cuts at: 4891 4891 Size: 7555 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2251 3433 2011 Size: 240 1182 6133 Fragments arranged by size: 6133 1182 240 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7555 XmaI C'CCGG_G Cuts at: 1870 3840 4380 4449 4791 4880 1870 Size: 1970 540 69 342 89 4545 Fragments arranged by size: 4545 1970 540 342 89 69 XmnI GAAnn'nnTTC Cuts at: 4007 5684 4007 Size: 1677 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5363 276 Size: 53 83 186 936 3829 2468 Fragments arranged by size: 3829 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AvrII BaeI BsiWI BstZ17I EcoRV FalI FseI FspAI NruI PacI PspXI PsrI RsrII SrfI SwaI