(Linear) (Six-base) MAPSORT of: KIEE6764.seq Check: 4933 from: 1 to: 2850 KIEE6764.orf With 182 enzymes: SgfI * March 16, 2015 11:36 .. Acc65I G'GTAC_C Cuts at: 0 1006 2850 Size: 1006 1844 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 269 2203 2850 Size: 269 1934 647 Fragments arranged by size: 1934 647 269 AflIII A'CryG_T Cuts at: 0 1094 1591 2850 Size: 1094 497 1259 Fragments arranged by size: 1259 1094 497 AhdI GACnn_n'nnGTC Cuts at: 0 2455 2850 Size: 2455 395 AleI CACnn'nnGTG Cuts at: 0 2293 2754 2850 Size: 2293 461 96 Fragments arranged by size: 2293 461 96 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2221 2284 2590 2850 Size: 2221 63 306 260 Fragments arranged by size: 2221 306 260 63 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 202 234 1975 2007 2850 Size: 202 32 1741 32 843 Fragments arranged by size: 1741 843 202 32 32 AlwNI CAG_nnn'CTG Cuts at: 0 960 2702 2823 2850 Size: 960 1742 121 27 Fragments arranged by size: 1742 960 121 27 ApaI G_GGCC'C Cuts at: 0 561 836 2850 Size: 561 275 2014 Fragments arranged by size: 2014 561 275 ApaLI G'TGCA_C Cuts at: 0 1407 1626 2850 Size: 1407 219 1224 Fragments arranged by size: 1407 1224 219 AscI GG'CGCG_CC Cuts at: 0 624 2850 Size: 624 2226 AvaI C'yCGr_G Cuts at: 0 133 531 919 1300 1835 2375 2411 2444 Size: 133 398 388 381 535 540 36 33 Cuts at: 2444 2786 2850 Size: 342 64 Fragments arranged by size: 540 535 398 388 381 342 133 64 36 33 BamHI G'GATC_C Cuts at: 0 1566 2850 Size: 1566 1284 BanI G'GyrC_C Cuts at: 0 391 1006 1315 1468 1809 1893 2113 2850 Size: 391 615 309 153 341 84 220 737 Fragments arranged by size: 737 615 391 341 309 220 153 84 BanII G_rGCy'C Cuts at: 0 272 283 368 561 836 1366 1706 1993 Size: 272 11 85 193 275 530 340 287 Cuts at: 1993 2605 2678 2696 2850 Size: 612 73 18 154 Fragments arranged by size: 612 530 340 287 275 272 193 154 85 73 18 11 BbeI G_GCGC'C Cuts at: 0 395 1319 2117 2850 Size: 395 924 798 733 Fragments arranged by size: 924 798 733 395 BbvCI CC'TCA_GC Cuts at: 0 2476 2850 Size: 2476 374 BfrBI ATG'CAT Cuts at: 0 2079 2850 Size: 2079 771 BglI GCCn_nnn'nGGC Cuts at: 0 2771 2850 Size: 2771 79 BlpI GC'TnA_GC Cuts at: 0 1716 2695 2850 Size: 1716 979 155 Fragments arranged by size: 1716 979 155 Bme1580I G_kGCm'C Cuts at: 0 561 836 1256 1411 1630 1814 2290 2850 Size: 561 275 420 155 219 184 476 560 Fragments arranged by size: 561 560 476 420 275 219 184 155 BmgBI CAC'GTC Cuts at: 0 318 2850 Size: 318 2532 BmrI ACTGGGnnnn_n' Cuts at: 0 936 2850 Size: 936 1914 BmtI G_CTAG'C Cuts at: 0 428 2850 Size: 428 2422 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 351 2367 2850 Size: 351 2016 483 Fragments arranged by size: 2016 483 351 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 247 454 1720 2347 2850 Size: 68 179 207 1266 627 503 Fragments arranged by size: 1266 627 503 207 179 68 Bpu10I CC'TnA_GC Cuts at: 0 1330 2476 2850 Size: 1330 1146 374 Fragments arranged by size: 1330 1146 374 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 2103 2737 2850 Size: 2103 634 113 Fragments arranged by size: 2103 634 113 BsaI GGTCTCn'nnnn_ Cuts at: 0 497 2850 Size: 497 2353 BsaAI yAC'GTr Cuts at: 0 657 1095 2850 Size: 657 438 1755 Fragments arranged by size: 1755 657 438 BsaHI Gr'CG_yC Cuts at: 0 205 254 392 1316 2114 2850 Size: 205 49 138 924 798 736 Fragments arranged by size: 924 798 736 205 138 49 BsaWI w'CCGG_w Cuts at: 0 499 1723 2850 Size: 499 1224 1127 Fragments arranged by size: 1224 1127 499 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 452 482 1661 1691 1732 1762 1921 1951 Size: 452 30 1179 30 41 30 159 30 Cuts at: 1951 1975 2005 2407 2437 2850 Size: 24 30 402 30 413 Fragments arranged by size: 1179 452 413 402 159 41 30 30 30 30 30 30 24 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1147 1756 2850 Size: 1147 609 1094 Fragments arranged by size: 1147 1094 609 BseYI C'CCAG_C Cuts at: 0 9 766 1086 1279 1711 2525 2540 2850 Size: 9 757 320 193 432 814 15 310 Fragments arranged by size: 814 757 432 320 310 193 15 9 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1377 1627 2504 2850 Size: 1377 250 877 346 Fragments arranged by size: 1377 877 346 250 BsiEI CG_ry'CG Cuts at: 0 111 1194 2730 2850 Size: 111 1083 1536 120 Fragments arranged by size: 1536 1083 120 111 BsiHKAI G_wGCw'C Cuts at: 0 1411 1630 1924 2554 2696 2850 Size: 1411 219 294 630 142 154 Fragments arranged by size: 1411 630 294 219 154 142 BsmBI CGTCTCn'nnnn_ Cuts at: 0 655 2850 Size: 655 2195 Bsp1286I G_dGCh'C Cuts at: 0 272 283 368 561 836 1256 1366 1411 Size: 272 11 85 193 275 420 110 45 Cuts at: 1411 1630 1706 1814 1924 1993 2290 2554 2605 Size: 219 76 108 110 69 297 264 51 Cuts at: 2605 2678 2696 2850 Size: 73 18 154 Fragments arranged by size: 420 297 275 272 264 219 193 154 110 110 108 85 76 73 69 51 45 18 11 BspEI T'CCGG_A Cuts at: 0 499 1723 2850 Size: 499 1224 1127 Fragments arranged by size: 1224 1127 499 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 299 605 2213 2850 Size: 299 306 1608 637 Fragments arranged by size: 1608 637 306 299 BsrBI CCG'CTC Cuts at: 0 66 2241 2850 Size: 66 2175 609 Fragments arranged by size: 2175 609 66 BsrDI GCAATG_nn' Cuts at: 0 855 2850 Size: 855 1995 BsrFI r'CCGG_y Cuts at: 0 432 608 844 1784 2774 2850 Size: 432 176 236 940 990 76 Fragments arranged by size: 990 940 432 236 176 76 BssHII G'CGCG_C Cuts at: 0 428 624 2850 Size: 428 196 2226 Fragments arranged by size: 2226 428 196 BssSI C'ACGA_G Cuts at: 0 1081 2850 Size: 1081 1769 BstAPI GCAn_nnn'nTGC Cuts at: 0 1493 1661 1919 2850 Size: 1493 168 258 931 Fragments arranged by size: 1493 931 258 168 BstEII G'GTnAC_C Cuts at: 0 840 2705 2850 Size: 840 1865 145 Fragments arranged by size: 1865 840 145 BstXI CCAn_nnnn'nTGG Cuts at: 0 90 1905 2493 2850 Size: 90 1815 588 357 Fragments arranged by size: 1815 588 357 90 BstYI r'GATC_y Cuts at: 0 1305 1566 2850 Size: 1305 261 1284 Fragments arranged by size: 1305 1284 261 Bsu36I CC'TnA_GG Cuts at: 0 2839 2850 Size: 2839 11 BtgI C'CryG_G Cuts at: 0 369 577 649 699 1799 2339 2354 2651 Size: 369 208 72 50 1100 540 15 297 Cuts at: 2651 2766 2850 Size: 115 84 Fragments arranged by size: 1100 540 369 297 208 115 84 72 50 15 BtsI GCAGTG_nn' Cuts at: 0 932 1073 1224 1345 1630 1753 2574 2850 Size: 932 141 151 121 285 123 821 276 Fragments arranged by size: 932 821 285 276 151 141 123 121 DraIII CAC_nnn'GTG Cuts at: 0 1542 1919 2850 Size: 1542 377 931 Fragments arranged by size: 1542 931 377 DrdI GACnn_nn'nnGTC Cuts at: 0 2833 2850 Size: 2833 17 EaeI y'GGCC_r Cuts at: 0 108 1613 2099 2727 2850 Size: 108 1505 486 628 123 Fragments arranged by size: 1505 628 486 123 108 EagI C'GGCC_G Cuts at: 0 108 2727 2850 Size: 108 2619 123 Fragments arranged by size: 2619 123 108 EarI CTCTTCn'nnn_ Cuts at: 0 288 949 1993 2068 2850 Size: 288 661 1044 75 782 Fragments arranged by size: 1044 782 661 288 75 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 2122 2595 2850 Size: 2122 473 255 Fragments arranged by size: 2122 473 255 EcoICRI GAG'CTC Cuts at: 0 2694 2850 Size: 2694 156 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 68 247 269 454 1720 2203 2347 2850 Size: 68 179 22 185 1266 483 144 503 Fragments arranged by size: 1266 503 483 185 179 144 68 22 EcoNI CCTnn'n_nnAGG Cuts at: 0 1300 2850 Size: 1300 1550 EcoO109I rG'GnC_Cy Cuts at: 0 130 558 957 1505 2244 2346 2439 2449 Size: 130 428 399 548 739 102 93 10 Cuts at: 2449 2513 2850 Size: 64 337 Fragments arranged by size: 739 548 428 399 337 130 102 93 64 10 FspI TGC'GCA Cuts at: 0 868 2850 Size: 868 1982 HaeII r_GCGC'y Cuts at: 0 395 477 1319 2117 2702 2748 2850 Size: 395 82 842 798 585 46 102 Fragments arranged by size: 842 798 585 395 102 82 46 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 496 528 811 843 1511 1543 1921 1953 Size: 496 32 283 32 668 32 378 32 Cuts at: 1953 2407 2410 2439 2441 2442 2473 2850 Size: 454 3 29 2 1 31 377 Fragments arranged by size: 668 496 454 378 377 283 32 32 32 32 31 29 3 2 1 KasI G'GCGC_C Cuts at: 0 391 1315 2113 2850 Size: 391 924 798 737 Fragments arranged by size: 924 798 737 391 KpnI G_GTAC'C Cuts at: 0 1010 2850 Size: 1010 1840 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 111 2510 2850 Size: 111 2399 340 Fragments arranged by size: 2399 340 111 MscI TGG'CCA Cuts at: 0 1615 2101 2850 Size: 1615 486 749 Fragments arranged by size: 1615 749 486 MslI CAynn'nnrTG Cuts at: 0 793 1115 2293 2754 2850 Size: 793 322 1178 461 96 Fragments arranged by size: 1178 793 461 322 96 MspA1I CmG'CkG Cuts at: 0 46 371 414 579 651 1250 1475 1611 Size: 46 325 43 165 72 599 225 136 Cuts at: 1611 2279 2293 2334 2540 2850 Size: 668 14 41 206 310 Fragments arranged by size: 668 599 325 310 225 206 165 136 72 46 43 41 14 NaeI GCC'GGC Cuts at: 0 434 610 2776 2850 Size: 434 176 2166 74 Fragments arranged by size: 2166 434 176 74 NarI GG'CG_CC Cuts at: 0 392 1316 2114 2850 Size: 392 924 798 736 Fragments arranged by size: 924 798 736 392 NgoMIV G'CCGG_C Cuts at: 0 432 608 2774 2850 Size: 432 176 2166 76 Fragments arranged by size: 2166 432 176 76 NheI G'CTAG_C Cuts at: 0 424 2850 Size: 424 2426 NsiI A_TGCA'T Cuts at: 0 2081 2850 Size: 2081 769 NspI r_CATG'y Cuts at: 0 1107 1386 1595 2850 Size: 1107 279 209 1255 Fragments arranged by size: 1255 1107 279 209 PasI CC'CwG_GG Cuts at: 0 987 1457 2850 Size: 987 470 1393 Fragments arranged by size: 1393 987 470 PciI A'CATG_T Cuts at: 0 1591 2850 Size: 1591 1259 PflMI CCAn_nnn'nTGG Cuts at: 0 1086 1205 1403 2823 2850 Size: 1086 119 198 1420 27 Fragments arranged by size: 1420 1086 198 119 27 PfoI T'CCnGG_A Cuts at: 0 1241 1268 1736 2324 2850 Size: 1241 27 468 588 526 Fragments arranged by size: 1241 588 526 468 27 PmlI CAC'GTG Cuts at: 0 1095 2850 Size: 1095 1755 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 1975 2007 2850 Size: 1975 32 843 Fragments arranged by size: 1975 843 32 PpuMI rG'GwC_Cy Cuts at: 0 130 957 2244 2346 2439 2449 2850 Size: 130 827 1287 102 93 10 401 Fragments arranged by size: 1287 827 401 130 102 93 10 PspOMI G'GGCC_C Cuts at: 0 557 832 2850 Size: 557 275 2018 Fragments arranged by size: 2018 557 275 PstI C_TGCA'G Cuts at: 0 1219 2309 2579 2850 Size: 1219 1090 270 271 Fragments arranged by size: 1219 1090 271 270 PvuII CAG'CTG Cuts at: 0 1475 1611 2540 2850 Size: 1475 136 929 310 Fragments arranged by size: 1475 929 310 136 SacI G_AGCT'C Cuts at: 0 2696 2850 Size: 2696 154 SacII CC_GC'GG Cuts at: 0 372 580 652 2850 Size: 372 208 72 2198 Fragments arranged by size: 2198 372 208 72 SanDI GG'GwC_CC Cuts at: 0 130 2449 2850 Size: 130 2319 401 Fragments arranged by size: 2319 401 130 SapI GCTCTTCn'nnn_ Cuts at: 0 288 2850 Size: 288 2562 SbfI CC_TGCA'GG Cuts at: 0 2309 2850 Size: 2309 541 ScaI AGT'ACT Cuts at: 0 925 1390 1660 1819 2850 Size: 925 465 270 159 1031 Fragments arranged by size: 1031 925 465 270 159 SexAI A'CCwGG_T Cuts at: 0 2246 2489 2850 Size: 2246 243 361 Fragments arranged by size: 2246 361 243 SfcI C'TryA_G Cuts at: 0 1215 2305 2575 2850 Size: 1215 1090 270 275 Fragments arranged by size: 1215 1090 275 270 SfiI GGCCn_nnn'nGGCC Cuts at: 0 2771 2850 Size: 2771 79 SfoI GGC'GCC Cuts at: 0 393 1317 2115 2850 Size: 393 924 798 735 Fragments arranged by size: 924 798 735 393 SgrAI Cr'CCGG_yG Cuts at: 0 432 2850 Size: 432 2418 SmaI CCC'GGG Cuts at: 0 1837 2377 2446 2788 2850 Size: 1837 540 69 342 62 Fragments arranged by size: 1837 540 342 69 62 SmlI C'TyrA_G Cuts at: 0 2118 2752 2850 Size: 2118 634 98 Fragments arranged by size: 2118 634 98 StyI C'CwwG_G Cuts at: 0 836 1421 1898 1950 2193 2435 2850 Size: 836 585 477 52 243 242 415 Fragments arranged by size: 836 585 477 415 243 242 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1797 1911 2517 2850 Size: 1797 114 606 333 Fragments arranged by size: 1797 606 333 114 TatI w'GTAC_w Cuts at: 0 923 1388 1658 1817 2850 Size: 923 465 270 159 1033 Fragments arranged by size: 1033 923 465 270 159 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 452 484 1153 1185 1418 1450 1661 1693 Size: 452 32 669 32 233 32 211 32 Cuts at: 1693 1732 1764 2850 Size: 39 32 1086 Fragments arranged by size: 1086 669 452 233 211 39 32 32 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 246 1428 2850 Size: 246 1182 1422 Fragments arranged by size: 1422 1182 246 XmaI C'CCGG_G Cuts at: 0 1835 2375 2444 2786 2850 Size: 1835 540 69 342 64 Fragments arranged by size: 1835 540 342 69 64 XmnI GAAnn'nnTTC Cuts at: 0 2002 2850 Size: 2002 848 Enzymes that do cut: Acc65I AcuI AflIII AhdI AleI AlfI AloI AlwNI ApaI ApaLI AscI AvaI BamHI BanI BanII BbeI BbvCI BfrBI BglI BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmBI Bsp1286I BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FspI HaeII Hin4I KasI KpnI MmeI MscI MslI MspA1I NaeI NarI NgoMIV NheI NsiI NspI PasI PciI PflMI PfoI PmlI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI SgrAI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AarI AatII AccI AclI AfeI AflII AgeI ApoI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BclI BglII BsaBI BsiWI BsmI BspHI BsrGI BstBI BstZ17I BtgZI ClaI CspCI DraI EcoRI EcoRV FalI FseI FspAI HincII HindIII HpaI MfeI MluI NcoI NdeI NotI NruI PacI PmeI PshAI PsiI PspXI PsrI PvuI RsrII SalI SgfI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI ZraI