(Circular) (Six-base) MAPSORT of: pFN21AE5061.seq Check: 1618 from: 1 to: 5878 pFN21AE5061 5878 bp With 182 enzymes: SgfI * July 19, 2019 13:42 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3688 278 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3221 1937 Size: 1284 4594 Acc65I G'GTAC_C Cuts at: 3199 3199 Size: 5878 AclI AA'CG_TT Cuts at: 1563 4005 4378 1563 Size: 2442 373 3063 Fragments arranged by size: 3063 2442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2717 2736 3941 5325 5593 1632 Size: 1085 19 1205 1384 268 1917 Fragments arranged by size: 1917 1384 1205 1085 268 19 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5681 AflIII A'CryG_T Cuts at: 1203 2034 4777 5754 1203 Size: 831 2743 977 1327 Fragments arranged by size: 2743 1327 977 831 AgeI A'CCGG_T Cuts at: 4709 4709 Size: 5878 AhdI GACnn_n'nnGTC Cuts at: 4607 4607 Size: 5878 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5878 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5878 AlwNI CAG_nnn'CTG Cuts at: 3621 5193 3621 Size: 1572 4306 ApaLI G'TGCA_C Cuts at: 3935 5091 3935 Size: 1156 4722 ApoI r'AATT_y Cuts at: 1493 2159 2336 2779 3187 3413 3565 5674 1493 Size: 666 177 443 408 226 152 2109 1697 Fragments arranged by size: 2109 1697 666 443 408 226 177 152 AseI AT'TA_AT Cuts at: 160 1032 2379 4432 160 Size: 872 1347 2053 1606 Fragments arranged by size: 2053 1606 1347 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 AvaI C'yCGr_G Cuts at: 1870 1943 3203 1870 Size: 73 1260 4545 Fragments arranged by size: 4545 1260 73 BamHI G'GATC_C Cuts at: 3208 3575 3208 Size: 367 5511 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3199 4654 618 Size: 325 217 627 1412 1455 1842 Fragments arranged by size: 1842 1455 1412 627 325 217 BanII G_rGCy'C Cuts at: 1391 3197 1391 Size: 1806 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2705 928 Size: 971 806 4101 Fragments arranged by size: 4101 971 806 BbvCI CC'TCA_GC Cuts at: 2392 2392 Size: 5878 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4069 4103 1903 Size: 34 2132 34 3678 Fragments arranged by size: 3678 2132 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3001 3770 4980 3001 Size: 769 1210 3899 Fragments arranged by size: 3899 1210 769 BclI T'GATC_A Cuts at: 1548 2040 2601 2821 1548 Size: 492 561 220 4605 Fragments arranged by size: 4605 561 492 220 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4489 136 Size: 107 122 71 631 605 2817 1525 Fragments arranged by size: 2817 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2547 5873 2547 Size: 3326 2552 BlpI GC'TnA_GC Cuts at: 3295 3295 Size: 5878 Bme1580I G_kGCm'C Cuts at: 1790 3939 5095 1790 Size: 2149 1156 2573 Fragments arranged by size: 2573 2149 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5878 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3609 4567 442 Size: 416 53 83 399 24 2192 958 1753 Fragments arranged by size: 2192 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5878 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2432 2536 2849 4538 1239 Size: 1193 104 313 1689 2579 Fragments arranged by size: 2579 1689 1193 313 104 Bpu10I CC'TnA_GC Cuts at: 2392 2392 Size: 5878 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2213 2403 4003 4868 5166 2213 Size: 190 1600 865 298 2925 Fragments arranged by size: 2925 1600 865 298 190 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2297 2717 2849 3050 4541 882 Size: 617 798 420 132 201 1491 2219 Fragments arranged by size: 2219 1491 798 617 420 201 132 BsaAI yAC'GTr Cuts at: 493 1204 3669 493 Size: 711 2465 2702 Fragments arranged by size: 2702 2465 711 BsaBI GATnn'nnATC Cuts at: 2747 5872 2747 Size: 3125 2753 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3685 4067 4702 275 Size: 53 83 186 936 2152 382 635 1451 Fragments arranged by size: 2152 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2934 4311 4709 4983 5130 5541 2934 Size: 1377 398 274 147 411 3271 Fragments arranged by size: 3271 1377 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2165 2195 2695 2725 1178 Size: 30 165 30 762 30 500 30 4331 Fragments arranged by size: 4331 762 500 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2969 1186 Size: 1783 4095 BseYI C'CCAG_C Cuts at: 2768 5081 5577 2768 Size: 2313 496 3069 Fragments arranged by size: 3069 2313 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5305 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3328 4089 5117 1540 Size: 275 186 1327 761 1028 2301 Fragments arranged by size: 2301 1327 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3939 4024 5095 3939 Size: 85 1071 4722 Fragments arranged by size: 4722 1071 85 BsmI GAATG_Cn' Cuts at: 2014 2107 3389 3482 2014 Size: 93 1282 93 4410 Fragments arranged by size: 4410 1282 93 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3197 3939 4024 5095 1391 Size: 399 1407 742 85 1071 2174 Fragments arranged by size: 2174 1407 1071 742 399 85 BspEI T'CCGG_A Cuts at: 2934 5541 2934 Size: 2607 3271 BspHI T'CATG_A Cuts at: 3767 5497 3767 Size: 1730 4148 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2933 3233 844 Size: 2089 300 3489 Fragments arranged by size: 3489 2089 300 BsrBI CCG'CTC Cuts at: 1394 1823 3765 1394 Size: 429 1942 3507 Fragments arranged by size: 3507 1942 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2173 4373 4547 65 Size: 1183 925 2200 174 1396 Fragments arranged by size: 2200 1396 1183 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4522 4709 1700 Size: 110 2712 187 2869 Fragments arranged by size: 2869 2712 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5878 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5878 BssSI C'ACGA_G Cuts at: 3938 4950 3938 Size: 1012 4866 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5878 BstBI TT'CG_AA Cuts at: 4682 4682 Size: 5878 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5878 BstXI CCAn_nnnn'nTGG Cuts at: 2734 2734 Size: 5878 BstYI r'GATC_y Cuts at: 1972 2547 3208 3575 3960 3977 5429 5515 5527 Size: 575 661 367 385 17 1452 86 12 Cuts at: 5527 5873 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 661 575 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5326 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5139 Fragments arranged by size: 5139 683 56 BtsI GCAGTG_nn' Cuts at: 2466 2604 3394 4188 4208 2466 Size: 138 790 794 20 4136 Fragments arranged by size: 4136 794 790 138 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5878 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5843 DraI TTT'AAA Cuts at: 3182 3182 Size: 5878 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4885 809 Size: 507 3569 1802 Fragments arranged by size: 3569 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3325 4214 5843 Size: 54 1425 50 275 18 1495 889 1629 Cuts at: 5843 8 Size: 43 Fragments arranged by size: 1629 1495 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3325 1537 Size: 275 1513 4090 Fragments arranged by size: 4090 1513 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2651 3107 3808 1353 Size: 1298 456 701 3423 Fragments arranged by size: 3423 1298 701 456 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4465 4839 4985 387 Size: 1064 138 2876 374 146 1280 Fragments arranged by size: 2876 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2432 2536 2717 2736 2849 3941 4538 Size: 393 800 104 181 19 113 1092 597 Cuts at: 4538 5325 5593 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1092 800 787 597 393 268 181 113 104 19 EcoRI G'AATT_C Cuts at: 2336 3187 3565 2336 Size: 851 378 4649 Fragments arranged by size: 4649 851 378 FspI TGC'GCA Cuts at: 4384 4384 Size: 5878 HaeII r_GCGC'y Cuts at: 1678 5025 1678 Size: 3347 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2165 2197 2496 2528 4519 Size: 32 135 32 593 32 299 32 1991 Cuts at: 4519 4551 4593 4625 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1991 593 299 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2052 2764 3222 3468 669 Size: 988 281 114 712 458 246 3079 Fragments arranged by size: 3079 988 712 458 281 246 114 HindIII A'AGCT_T Cuts at: 748 2621 2690 748 Size: 1873 69 3936 Fragments arranged by size: 3936 1873 69 HpaI GTT'AAC Cuts at: 3468 3468 Size: 5878 KpnI G_GTAC'C Cuts at: 3203 3203 Size: 5878 MfeI C'AATT_G Cuts at: 3477 3477 Size: 5878 MluI A'CGCG_T Cuts at: 5754 5754 Size: 5878 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2000 4992 5176 1305 Size: 695 2992 184 2007 Fragments arranged by size: 2992 2007 695 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4056 Fragments arranged by size: 4056 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2682 3173 3836 4195 4354 518 Size: 648 1516 491 663 359 159 2042 Fragments arranged by size: 2042 1516 663 648 491 359 159 MspA1I CmG'CkG Cuts at: 1606 2852 3294 3971 5119 5364 1606 Size: 1246 442 677 1148 245 2120 Fragments arranged by size: 2120 1246 1148 677 442 245 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5326 NdeI CA'TA_TG Cuts at: 387 387 Size: 5878 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5878 NotI GC'GGCC_GC Cuts at: 3325 3325 Size: 5878 NspI r_CATG'y Cuts at: 1132 1229 3236 4781 1132 Size: 97 2007 1545 2229 Fragments arranged by size: 2229 2007 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5878 PciI A'CATG_T Cuts at: 4777 4777 Size: 5878 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5878 PfoI T'CCnGG_A Cuts at: 2331 2331 Size: 5878 PmeI GTTT'AAAC Cuts at: 3182 3182 Size: 5878 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3947 3979 5460 5492 3947 Size: 32 1481 32 4333 Fragments arranged by size: 4333 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2705 1613 Size: 1092 4786 PsiI TTA'TAA Cuts at: 2510 3084 3448 2510 Size: 574 364 4940 Fragments arranged by size: 4940 574 364 PstI C_TGCA'G Cuts at: 830 3230 830 Size: 2400 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5878 SalI G'TCGA_C Cuts at: 1936 3220 1936 Size: 1284 4594 SapI GCTCTTCn'nnn_ Cuts at: 2651 2651 Size: 5878 SbfI CC_TGCA'GG Cuts at: 3230 3230 Size: 5878 ScaI AGT'ACT Cuts at: 4126 5838 4126 Size: 1712 4166 SfcI C'TryA_G Cuts at: 826 1045 3226 4361 5042 5233 826 Size: 219 2181 1135 681 191 1471 Fragments arranged by size: 2181 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5878 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5878 SmaI CCC'GGG Cuts at: 1872 3205 1872 Size: 1333 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2228 2418 3982 4883 5145 820 Size: 197 926 285 190 1564 901 262 1553 Fragments arranged by size: 1564 1553 926 901 285 262 197 190 SnaBI TAC'GTA Cuts at: 493 3669 493 Size: 3176 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5878 SphI G_CATG'C Cuts at: 1132 3236 1132 Size: 2104 3774 SspI AAT'ATT Cuts at: 5 52 2377 3802 5 Size: 47 2325 1425 2081 Fragments arranged by size: 2325 2081 1425 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5878 StyI C'CwwG_G Cuts at: 513 1065 3317 513 Size: 552 2252 3074 Fragments arranged by size: 3074 2252 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3905 3922 4075 4260 4705 325 Size: 1255 2325 17 153 185 445 1498 Fragments arranged by size: 2325 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4124 5836 96 Size: 274 80 33 51 1443 2147 1712 138 Fragments arranged by size: 2147 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2695 2727 5838 5870 2695 Size: 32 3111 32 2703 Fragments arranged by size: 3111 2703 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5878 XbaI T'CTAG_A Cuts at: 3214 3214 Size: 5878 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5878 XmaI C'CCGG_G Cuts at: 1870 3203 1870 Size: 1333 4545 XmnI GAAnn'nnTTC Cuts at: 4007 4007 Size: 5878 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3686 276 Size: 53 83 186 936 2152 2468 Fragments arranged by size: 2468 2152 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI