################################## # NO CUT ENZYMES ( 125 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AvrII BbrPI BbsI BcgI BciVI BfrI BglII BlnI BpuAI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI HincII HindII HindIII HpaI KpnI KspI MamI MfeI MluI MroI MseI MunI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TfiI Tru9I Tsp45I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 58 enzymes) # ################################### AccII 21 CGCG CG^CG AcsI 293 GAATTC [GA]^AATT[CT] AcyI 6 GGCGCC G[GA]^CG[CT]C AlwNI 892 CAGAAGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 27 GGGCCC GGGCC^C ApoI 293 GAATTC [GA]^AATT[CT] AspI 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 717 TGCGCA TGC^GCA BalI 417 TGGCCA TGG^CCA BamHI 35 GGATCC G^GATCC BbeI 6 GGCGCC GGCGC^C BbvCI 584 CCTCAGC CCTCAGC(-5/-2) BclI 855 TGATCA T^GATCA BfaI 928 CTAG C^TAG BglI 109 GCCTCTACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 747 GCTCAGC GC^T[ACGT]AGC BpmI 781 CTCCAG CTGGAG(16/14) Bpu1102I 747 GCTCAGC GC^T[ACGT]AGC BsaHI 6 GGCGCC G[GA]^CG[CT]C BsaI 340 GGTCTC GGTCTC(1/5) BseRI 762 CTCCTC GAGGAG(10/8) BsmBI 78 CGTCTC CGTCTC(1/5) BspMI 962 ACCTGC ACCTGC(4/8) BssSI 772 CTCGTG CACGAG(-5/-1) BstUI 21 CGCG CG^CG BstXI 1112 CCAAGTGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 35 GGATCC [GA]^GATC[CT] CelII 747 GCTCAGC GC^T[ACGT]AGC DraII 27 GGGCCCC [GA]G^G[ACGT]CC[CT] EarI 67 CTCTTC CTCTTC(1/4) EcoO109I 27 GGGCCCC [GA]G^G[ACGT]CC[CT] EcoRI 293 GAATTC G^AATTC FbaI 855 TGATCA T^GATCA FspI 717 TGCGCA TGC^GCA HaeII 6 GGCGCC [GA]GCGC^[CT] HgaI 22 GCGTC GACGC(5/10) Hin1I 6 GGCGCC G[GA]^CG[CT]C KasI 6 GGCGCC G^GCGCC Ksp632I 67 CTCTTC CTCTTC(1/4) MaeI 928 CTAG C^TAG MaeII 266 ACGT A^CGT MaeIII 730 GTAAC ^GT[ACGT]AC MflI 35 GGATCC [GA]^GATC[CT] MluNI 417 TGGCCA TGG^CCA MscI 417 TGGCCA TGG^CCA MslI 561 CATCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 21 CGCG CG^CG NarI 6 GGCGCC GG^CGCC NspI 570 GCATGT [GA]CATG^[CT] PflFI 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 247 CTGCAG CTGCA^G SacI 778 GAGCTC GAGCT^C SexAI 740 ACCAGGT A^CC[AT]GGT SfcI 247 CTGCAG C^T[GA][CT]AG SfoI 6 GGCGCC GGC^GCC Tsp509I 294 AATT ^AATT Tth111I 654 GACATTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 35 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AvaI 287 CCCGAG C^[CT]CG[GA]G 663 CCCGAG AvaII 366 GGTCC G^G[AT]CC 998 GGTCC BanI 6 GGCGCC G^G[CT][GA]CC 907 GGCACC BsgI 964 CTGCAC GTGCAG(16/14) 1057 CTGCAC BsmI 714 GAATGC GAATGC(1/-1) 1050 GCATTC BsoBI 287 CCCGAG C^[CT]CG[GA]G 663 CCCGAG BsrFI 186 GCCGGT [GA]^CCGG[CT] 449 ACCGGC BstAPI 1050 GCATTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1059 GCACCGCCTGC BstF5I 603 CATCC GGATG(2/0) 951 CATCC Cfr10I 186 GCCGGT [GA]^CCGG[CT] 449 ACCGGC EcoT14I 51 CCAAGG C^C[AT][AT]GG 849 CCTTGG FauI 944 CCCGC CCCGC(4/6) 59 GCGGG FokI 603 CATCC GGATG(9/13) 951 CATCC PflMI 447 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 844 CCAATCCTTGG PvuII 244 CAGCTG CAG^CTG 976 CAGCTG ScaI 551 AGTACT AGT^ACT 863 AGTACT StyI 51 CCAAGG C^C[AT][AT]GG 849 CCTTGG TaqI 813 TCGA T^CGA 937 TCGA TthHB8I 813 TCGA T^CGA 937 TCGA Van91I 447 CCACCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 844 CCAATCCTTGG XcmI 467 CCACAGGTGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 783 CCAGCCCATCACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 54 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI BanII BbvI BcnI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstNI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MboI MboII MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI TseI TspRI