(Circular) (Six-base) MAPSORT of: pFN21AE5557.seq Check: 2900 from: 1 to: 5836 pFN21AE5557 5836 bp With 182 enzymes: SgfI * January 30, 2014 17:22 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2976 2976 Size: 5836 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3646 278 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3179 1937 Size: 1242 4594 Acc65I G'GTAC_C Cuts at: 3157 3157 Size: 5836 AclI AA'CG_TT Cuts at: 1563 3963 4336 1563 Size: 2400 373 3063 Fragments arranged by size: 3063 2400 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2939 3899 5283 5551 1632 Size: 1307 960 1384 268 1917 Fragments arranged by size: 1917 1384 1307 960 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5639 AflIII A'CryG_T Cuts at: 1203 4735 5712 1203 Size: 3532 977 1327 Fragments arranged by size: 3532 1327 977 AgeI A'CCGG_T Cuts at: 4667 4667 Size: 5836 AhdI GACnn_n'nnGTC Cuts at: 4565 4565 Size: 5836 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5836 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5836 AlwNI CAG_nnn'CTG Cuts at: 2902 3579 5151 2902 Size: 677 1572 3587 Fragments arranged by size: 3587 1572 677 ApaI G_GGCC'C Cuts at: 2036 2036 Size: 5836 ApaLI G'TGCA_C Cuts at: 3893 5049 3893 Size: 1156 4680 ApoI r'AATT_y Cuts at: 1493 2298 3145 3371 3523 5632 1493 Size: 805 847 226 152 2109 1697 Fragments arranged by size: 2109 1697 847 805 226 152 AseI AT'TA_AT Cuts at: 160 1032 4390 160 Size: 872 3358 1606 Fragments arranged by size: 3358 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 AvaI C'yCGr_G Cuts at: 1870 1943 2292 2668 3161 1870 Size: 73 349 376 493 4545 Fragments arranged by size: 4545 493 376 349 73 BamHI G'GATC_C Cuts at: 2040 3166 3533 2040 Size: 1126 367 4343 Fragments arranged by size: 4343 1126 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2011 2912 3157 4612 618 Size: 325 217 627 224 901 245 1455 1842 Fragments arranged by size: 1842 1455 901 627 325 245 224 217 BanII G_rGCy'C Cuts at: 1391 2036 2161 2439 2787 3155 1391 Size: 645 125 278 348 368 4072 Fragments arranged by size: 4072 645 368 348 278 125 BbeI G_GCGC'C Cuts at: 2015 2015 Size: 5836 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4865 BbvCI CC'TCA_GC Cuts at: 2590 2590 Size: 5836 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4027 4061 1903 Size: 34 2090 34 3678 Fragments arranged by size: 3678 2090 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3728 4938 3728 Size: 1210 4626 BclI T'GATC_A Cuts at: 1548 2860 1548 Size: 1312 4524 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2009 2120 4447 Size: 107 122 71 631 605 337 111 2327 Cuts at: 4447 136 Size: 1525 Fragments arranged by size: 2327 1525 631 605 337 122 111 107 71 BglII A'GATC_T Cuts at: 5831 5831 Size: 5836 BlpI GC'TnA_GC Cuts at: 2753 3253 2753 Size: 500 5336 Bme1580I G_kGCm'C Cuts at: 1790 2036 2915 3897 5053 1790 Size: 246 879 982 1156 2573 Fragments arranged by size: 2573 1156 982 879 246 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5836 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2569 3567 4525 Size: 416 53 83 399 24 1152 998 958 Cuts at: 4525 442 Size: 1753 Fragments arranged by size: 1753 1152 998 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5836 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2318 2318 Size: 5836 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2771 4496 1239 Size: 1532 1725 2579 Fragments arranged by size: 2579 1725 1532 Bpu10I CC'TnA_GC Cuts at: 2280 2590 2280 Size: 310 5526 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3961 4826 5124 3961 Size: 865 298 4673 Fragments arranged by size: 4673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2351 4499 882 Size: 617 852 2148 2219 Fragments arranged by size: 2219 2148 852 617 BsaAI yAC'GTr Cuts at: 493 1204 3627 493 Size: 711 2423 2702 Fragments arranged by size: 2702 2423 711 BsaBI GATnn'nnATC Cuts at: 5830 5830 Size: 5836 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3643 4025 4660 Size: 53 83 186 936 479 1631 382 635 Cuts at: 4660 275 Size: 1451 Fragments arranged by size: 1631 1451 936 635 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4269 4667 4941 5088 5499 4269 Size: 398 274 147 411 4606 Fragments arranged by size: 4606 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2180 2210 3120 3150 1178 Size: 30 165 30 777 30 910 30 3864 Fragments arranged by size: 3864 910 777 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2758 1186 Size: 1572 4264 BseYI C'CCAG_C Cuts at: 2926 5039 5535 2926 Size: 2113 496 3227 Fragments arranged by size: 3227 2113 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2954 3047 1163 Size: 573 1218 93 3952 Fragments arranged by size: 3952 1218 573 93 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3286 4047 5075 1540 Size: 275 186 1285 761 1028 2301 Fragments arranged by size: 2301 1285 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2754 2787 3133 3897 3982 5053 2754 Size: 33 346 764 85 1071 3537 Fragments arranged by size: 3537 1071 764 346 85 33 BsmI GAATG_Cn' Cuts at: 2725 3055 3347 3440 2725 Size: 330 292 93 5121 Fragments arranged by size: 5121 330 292 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2089 2089 Size: 5836 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2036 2161 2439 2754 2787 2915 3133 Size: 399 246 125 278 315 33 128 218 Cuts at: 3133 3155 3897 3982 5053 1391 Size: 22 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 315 278 246 218 128 125 85 33 22 BspEI T'CCGG_A Cuts at: 5499 5499 Size: 5836 BspHI T'CATG_A Cuts at: 3725 5455 3725 Size: 1730 4106 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2976 3191 844 Size: 2132 215 3489 Fragments arranged by size: 3489 2132 215 BsrBI CCG'CTC Cuts at: 1394 1823 3723 1394 Size: 429 1900 3507 Fragments arranged by size: 3507 1900 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4331 4505 65 Size: 1183 3083 174 1396 Fragments arranged by size: 3083 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2191 2454 4480 4667 1700 Size: 110 381 263 2026 187 2869 Fragments arranged by size: 2869 2026 381 263 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5836 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5836 BssSI C'ACGA_G Cuts at: 2777 3896 4908 2777 Size: 1119 1012 3705 Fragments arranged by size: 3705 1119 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3061 3070 794 Size: 2267 9 3560 Fragments arranged by size: 3560 2267 9 BstBI TT'CG_AA Cuts at: 4640 4640 Size: 5836 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5836 BstXI CCAn_nnnn'nTGG Cuts at: 3124 3124 Size: 5836 BstYI r'GATC_y Cuts at: 1972 2040 3166 3533 3918 3935 5387 5473 5485 Size: 68 1126 367 385 17 1452 86 12 Cuts at: 5485 5831 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1126 385 367 346 86 68 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5097 Fragments arranged by size: 5097 683 56 BtsI GCAGTG_nn' Cuts at: 3352 4146 4166 3352 Size: 794 20 5022 Fragments arranged by size: 5022 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5836 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5801 DraI TTT'AAA Cuts at: 3140 3140 Size: 5836 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4843 809 Size: 507 3527 1802 Fragments arranged by size: 3527 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2053 2121 2422 Size: 54 1425 50 275 18 223 68 301 Cuts at: 2422 3283 4172 5801 8 Size: 861 889 1629 43 Fragments arranged by size: 1629 1425 889 861 301 275 223 68 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3283 1537 Size: 275 1471 4090 Fragments arranged by size: 4090 1471 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2078 3766 1353 Size: 725 1688 3423 Fragments arranged by size: 3423 1688 725 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2761 4423 4797 4943 387 Size: 1064 138 1172 1662 374 146 1280 Fragments arranged by size: 1662 1280 1172 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2785 2785 Size: 5836 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2771 2939 3899 4496 5283 5551 1239 Size: 393 1139 168 960 597 787 268 1524 Fragments arranged by size: 1524 1139 960 787 597 393 268 168 EcoO109I rG'GnC_Cy Cuts at: 2033 2033 Size: 5836 EcoRI G'AATT_C Cuts at: 2298 3145 3523 2298 Size: 847 378 4611 Fragments arranged by size: 4611 847 378 FspI TGC'GCA Cuts at: 2724 4342 2724 Size: 1618 4218 HaeII r_GCGC'y Cuts at: 1678 2015 4983 1678 Size: 337 2968 2531 Fragments arranged by size: 2968 2531 337 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2180 2212 4477 4509 4551 Size: 32 135 32 608 32 2265 32 42 Cuts at: 4551 4583 1373 Size: 32 2626 Fragments arranged by size: 2626 2265 608 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3180 3426 669 Size: 988 281 1242 246 3079 Fragments arranged by size: 3079 1242 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5836 HpaI GTT'AAC Cuts at: 3426 3426 Size: 5836 KasI G'GCGC_C Cuts at: 2011 2011 Size: 5836 KpnI G_GTAC'C Cuts at: 3161 3161 Size: 5836 MfeI C'AATT_G Cuts at: 3435 3435 Size: 5836 MluI A'CGCG_T Cuts at: 5712 5712 Size: 5836 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4950 5134 1305 Size: 3645 184 2007 Fragments arranged by size: 3645 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2424 10 Size: 54 1425 343 592 3422 Fragments arranged by size: 3422 1425 592 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2570 3794 4153 4312 518 Size: 648 1404 1224 359 159 2042 Fragments arranged by size: 2042 1404 1224 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2105 2111 2215 2251 2983 3252 3929 5077 Size: 499 6 104 36 732 269 677 1148 Cuts at: 5077 5322 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 732 677 499 269 245 104 36 6 NarI GG'CG_CC Cuts at: 2012 2012 Size: 5836 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4345 Fragments arranged by size: 4345 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5836 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5836 NotI GC'GGCC_GC Cuts at: 3283 3283 Size: 5836 NspI r_CATG'y Cuts at: 1132 1229 2579 3194 4739 1132 Size: 97 1350 615 1545 2229 Fragments arranged by size: 2229 1545 1350 615 97 PasI CC'CwG_GG Cuts at: 1348 2366 2998 1348 Size: 1018 632 4186 Fragments arranged by size: 4186 1018 632 PciI A'CATG_T Cuts at: 4735 4735 Size: 5836 PflMI CCAn_nnn'nTGG Cuts at: 1108 2458 2855 1108 Size: 1350 397 4089 Fragments arranged by size: 4089 1350 397 PfoI T'CCnGG_A Cuts at: 3005 3005 Size: 5836 PmeI GTTT'AAAC Cuts at: 3140 3140 Size: 5836 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3905 3937 5418 5450 3905 Size: 32 1481 32 4291 Fragments arranged by size: 4291 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5836 PsiI TTA'TAA Cuts at: 3406 3406 Size: 5836 PspOMI G'GGCC_C Cuts at: 2032 2032 Size: 5836 PstI C_TGCA'G Cuts at: 830 2256 3188 830 Size: 1426 932 3478 Fragments arranged by size: 3478 1426 932 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5836 PvuII CAG'CTG Cuts at: 2251 2983 2251 Size: 732 5104 SacI G_AGCT'C Cuts at: 2787 2787 Size: 5836 SalI G'TCGA_C Cuts at: 1936 3178 1936 Size: 1242 4594 SbfI CC_TGCA'GG Cuts at: 3188 3188 Size: 5836 ScaI AGT'ACT Cuts at: 2558 2870 4084 5796 2558 Size: 312 1214 1712 2598 Fragments arranged by size: 2598 1712 1214 312 SexAI A'CCwGG_T Cuts at: 2745 2745 Size: 5836 SfcI C'TryA_G Cuts at: 826 1045 2252 3184 4319 5000 5191 826 Size: 219 1207 932 1135 681 191 1471 Fragments arranged by size: 1471 1207 1135 932 681 219 191 SfoI GGC'GCC Cuts at: 2013 2013 Size: 5836 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5836 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5836 SmaI CCC'GGG Cuts at: 1872 3163 1872 Size: 1291 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3940 4841 5103 820 Size: 197 926 1997 901 262 1553 Fragments arranged by size: 1997 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3627 493 Size: 3134 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5836 SphI G_CATG'C Cuts at: 1132 3194 1132 Size: 2062 3774 SspI AAT'ATT Cuts at: 5 52 3760 5 Size: 47 3708 2081 Fragments arranged by size: 3708 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5836 StyI C'CwwG_G Cuts at: 513 1065 2004 2056 2854 3275 513 Size: 552 939 52 798 421 3074 Fragments arranged by size: 3074 939 798 552 421 52 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3863 3880 4033 4218 4663 325 Size: 1255 2283 17 153 185 445 1498 Fragments arranged by size: 2283 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2392 2556 2868 Size: 274 80 33 51 1443 415 164 312 Cuts at: 2868 4082 5794 96 Size: 1214 1712 138 Fragments arranged by size: 1712 1443 1214 415 312 274 164 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2502 2534 5796 5828 2502 Size: 32 3262 32 2510 Fragments arranged by size: 3262 2510 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2662 1316 Size: 1346 4490 XbaI T'CTAG_A Cuts at: 3172 3172 Size: 5836 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2479 2795 2479 Size: 316 5520 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5836 XmaI C'CCGG_G Cuts at: 1870 3161 1870 Size: 1291 4545 XmnI GAAnn'nnTTC Cuts at: 3965 3965 Size: 5836 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3644 276 Size: 53 83 186 936 2110 2468 Fragments arranged by size: 2468 2110 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I DraIII EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SfiI SrfI SwaI