(Linear) (Six-base) MAPSORT of: KIEE5557.seq Check: 7444 from: 1 to: 1131 KIEE5557.orf With 182 enzymes: SgfI * January 29, 2014 12:05 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 971 1131 Size: 971 160 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 934 1131 Size: 934 197 AlwNI CAG_nnn'CTG Cuts at: 0 897 1131 Size: 897 234 ApaI G_GGCC'C Cuts at: 0 31 1131 Size: 31 1100 ApoI r'AATT_y Cuts at: 0 293 1131 Size: 293 838 AvaI C'yCGr_G Cuts at: 0 287 663 1131 Size: 287 376 468 Fragments arranged by size: 468 376 287 BamHI G'GATC_C Cuts at: 0 35 1131 Size: 35 1096 BanI G'GyrC_C Cuts at: 0 6 907 1131 Size: 6 901 224 Fragments arranged by size: 901 224 6 BanII G_rGCy'C Cuts at: 0 31 156 434 782 1131 Size: 31 125 278 348 349 Fragments arranged by size: 349 348 278 125 31 BbeI G_GCGC'C Cuts at: 0 10 1131 Size: 10 1121 BbvCI CC'TCA_GC Cuts at: 0 585 1131 Size: 585 546 BclI T'GATC_A Cuts at: 0 855 1131 Size: 855 276 BglI GCCn_nnn'nGGC Cuts at: 0 115 1131 Size: 115 1016 BlpI GC'TnA_GC Cuts at: 0 748 1131 Size: 748 383 Bme1580I G_kGCm'C Cuts at: 0 31 910 1131 Size: 31 879 221 Fragments arranged by size: 879 221 31 BmrI ACTGGGnnnn_n' Cuts at: 0 564 1131 Size: 564 567 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 313 1131 Size: 313 818 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 1131 Size: 766 365 Bpu10I CC'TnA_GC Cuts at: 0 275 585 1131 Size: 275 310 546 Fragments arranged by size: 546 310 275 BsaI GGTCTCn'nnnn_ Cuts at: 0 346 1131 Size: 346 785 BsaHI Gr'CG_yC Cuts at: 0 7 1131 Size: 7 1124 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 175 205 1131 Size: 175 30 926 Fragments arranged by size: 926 175 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 753 1131 Size: 753 378 BseYI C'CCAG_C Cuts at: 0 921 1131 Size: 921 210 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 949 1042 1131 Size: 949 93 89 Fragments arranged by size: 949 93 89 BsiHKAI G_wGCw'C Cuts at: 0 749 782 1128 1131 Size: 749 33 346 3 Fragments arranged by size: 749 346 33 3 BsmI GAATG_Cn' Cuts at: 0 720 1050 1131 Size: 720 330 81 Fragments arranged by size: 720 330 81 BsmBI CGTCTCn'nnnn_ Cuts at: 0 84 1131 Size: 84 1047 Bsp1286I G_dGCh'C Cuts at: 0 31 156 434 749 782 910 1128 1131 Size: 31 125 278 315 33 128 218 3 Fragments arranged by size: 315 278 218 128 125 33 31 3 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 971 1131 Size: 971 160 BsrFI r'CCGG_y Cuts at: 0 186 449 1131 Size: 186 263 682 Fragments arranged by size: 682 263 186 BssSI C'ACGA_G Cuts at: 0 772 1131 Size: 772 359 BstAPI GCAn_nnn'nTGC Cuts at: 0 1056 1065 1131 Size: 1056 9 66 Fragments arranged by size: 1056 66 9 BstXI CCAn_nnnn'nTGG Cuts at: 0 1119 1131 Size: 1119 12 BstYI r'GATC_y Cuts at: 0 35 1131 Size: 35 1096 EaeI y'GGCC_r Cuts at: 0 48 116 417 1131 Size: 48 68 301 714 Fragments arranged by size: 714 301 68 48 EarI CTCTTCn'nnn_ Cuts at: 0 73 1131 Size: 73 1058 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 756 1131 Size: 756 375 EcoICRI GAG'CTC Cuts at: 0 780 1131 Size: 780 351 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 766 934 1131 Size: 766 168 197 Fragments arranged by size: 766 197 168 EcoO109I rG'GnC_Cy Cuts at: 0 28 1131 Size: 28 1103 EcoRI G'AATT_C Cuts at: 0 293 1131 Size: 293 838 FspI TGC'GCA Cuts at: 0 719 1131 Size: 719 412 HaeII r_GCGC'y Cuts at: 0 10 1131 Size: 10 1121 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 175 207 1131 Size: 175 32 924 Fragments arranged by size: 924 175 32 KasI G'GCGC_C Cuts at: 0 6 1131 Size: 6 1125 MscI TGG'CCA Cuts at: 0 419 1131 Size: 419 712 MslI CAynn'nnrTG Cuts at: 0 565 1131 Size: 565 566 MspA1I CmG'CkG Cuts at: 0 100 106 210 246 978 1131 Size: 100 6 104 36 732 153 Fragments arranged by size: 732 153 104 100 36 6 NarI GG'CG_CC Cuts at: 0 7 1131 Size: 7 1124 NspI r_CATG'y Cuts at: 0 574 1131 Size: 574 557 PasI CC'CwG_GG Cuts at: 0 361 993 1131 Size: 361 632 138 Fragments arranged by size: 632 361 138 PflMI CCAn_nnn'nTGG Cuts at: 0 453 850 1131 Size: 453 397 281 Fragments arranged by size: 453 397 281 PfoI T'CCnGG_A Cuts at: 0 1000 1131 Size: 1000 131 PspOMI G'GGCC_C Cuts at: 0 27 1131 Size: 27 1104 PstI C_TGCA'G Cuts at: 0 251 1131 Size: 251 880 PvuII CAG'CTG Cuts at: 0 246 978 1131 Size: 246 732 153 Fragments arranged by size: 732 246 153 SacI G_AGCT'C Cuts at: 0 782 1131 Size: 782 349 ScaI AGT'ACT Cuts at: 0 553 865 1131 Size: 553 312 266 Fragments arranged by size: 553 312 266 SexAI A'CCwGG_T Cuts at: 0 740 1131 Size: 740 391 SfcI C'TryA_G Cuts at: 0 247 1131 Size: 247 884 SfoI GGC'GCC Cuts at: 0 8 1131 Size: 8 1123 StyI C'CwwG_G Cuts at: 0 51 849 1131 Size: 51 798 282 Fragments arranged by size: 798 282 51 TatI w'GTAC_w Cuts at: 0 387 551 863 1131 Size: 387 164 312 268 Fragments arranged by size: 387 312 268 164 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 497 529 1131 Size: 497 32 602 Fragments arranged by size: 602 497 32 Tth111I GACn'n_nGTC Cuts at: 0 657 1131 Size: 657 474 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 474 790 1131 Size: 474 316 341 Fragments arranged by size: 474 341 316 Enzymes that do cut: AarI AcuI AlwNI ApaI ApoI AvaI BamHI BanI BanII BbeI BbvCI BclI BglI BlpI Bme1580I BmrI BplI BpmI Bpu10I BsaI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspMI BsrFI BssSI BstAPI BstXI BstYI EaeI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I KasI MscI MslI MspA1I NarI NspI PasI PflMI PfoI PspOMI PstI PvuII SacI ScaI SexAI SfcI SfoI StyI TatI TstI Tth111I XcmI Enzymes that do not cut: AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BbsI BcgI BciVI BfrBI BglII BmgBI BmtI BpuEI BsaAI BsaBI BsaWI BsiEI BsiWI BspEI BspHI BsrBI BsrDI BsrGI BssHII BstBI BstEII BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI EcoNI EcoRV FalI FseI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII SacII SalI SanDI SapI SbfI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII XbaI XhoI XmaI XmnI ZraI