################################## # NO CUT ENZYMES ( 181 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcsI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaII AviII AvrII BalI BamHI BbrPI BbsI BbvCI BcgI BciVI BclI BfrI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmFI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DdeI DraI DraIII DrdI DsaI Eam1105I EarI Eco47III Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT14I EcoT22I FbaI FseI FspI HgaI HincII HindII HindIII HinfI HpaI HphI KpnI Ksp632I KspI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MunI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfuI SgrAI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI TaqI TfiI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XcmI XhoI XhoII XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AcyI 73 GGCGCC G[GA]^CG[CT]C AluI 189 AGCT AG^CT AlwI 67 GATCC GGATC(4/5) BanI 73 GGCGCC G^G[CT][GA]CC BbeI 73 GGCGCC GGCGC^C BbvI 8 GCTGC GCAGC(8/12) BfaI 49 CTAG C^TAG BsaHI 73 GGCGCC G[GA]^CG[CT]C BsgI 9 CTGCAC GTGCAG(16/14) BsiYI 166 CCGGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 166 CCGGGCCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsmAI 230 GAGAC GTCTC(1/5) BsrBI 141 GAGCGG CCGCTC(-3/-3) BstF5I 54 CATCC GGATG(2/0) DpnII 67 GATC ^GATC FokI 54 CATCC GGATG(9/13) HaeII 73 GGCGCC [GA]GCGC^[CT] Hin1I 73 GGCGCC G[GA]^CG[CT]C KasI 73 GGCGCC G^GCGCC MaeI 49 CTAG C^TAG MboI 67 GATC ^GATC MnlI 206 GAGG CCTC(7/6) NarI 73 GGCGCC GG^CGCC NdeII 67 GATC ^GATC NlaIII 120 CATG CATG^ NotI 82 GCGGCCGC GC^GGCCGC Sau3AI 67 GATC ^GATC SfiI 154 GGCCGGAAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 73 GGCGCC GGC^GCC TseI 8 GCTGC G^C[AT]GC XbaI 48 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### ApaI 162 GGGCCC GGGCC^C 168 GGGCCC AvaI 165 CCCGGG C^[CT]CG[GA]G 200 CCCGGG BanII 162 GGGCCC G[GA]GC[CT]^C 168 GGGCCC BglI 76 GCCAACGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 155 GCCGGAAGGGC BmyI 162 GGGCCC G[AGT]GC[ACT]^C 168 GGGCCC BsiEI 83 CGGCCG CG[GA][CT]^CG 153 CGGCCG BsoBI 165 CCCGGG C^[CT]CG[GA]G 200 CCCGGG Bsp1286I 162 GGGCCC G[AGT]GC[ACT]^C 168 GGGCCC Cac8I 22 GCCTGC GC[ACGT]^[ACGT]GC 211 GCGGGC EaeI 83 CGGCCG [CT]^GGCC[GA] 153 CGGCCG EagI 83 CGGCCG C^GGCCG 153 CGGCCG EclXI 83 CGGCCG C^GGCCG 153 CGGCCG Eco52I 83 CGGCCG C^GGCCG 153 CGGCCG FauI 57 CCCGC CCCGC(4/6) 211 GCGGG MboII 129 GAAGA GAAGA(8/7) 132 GAAGA MspA1I 58 CCGCTG C[AC]G^C[GT]G 225 CAGCGG SmaI 165 CCCGGG CCC^GGG 200 CCCGGG XmaCI 165 CCCGGG C^CCGGG 200 CCCGGG XmaI 165 CCCGGG C^CCGGG 200 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 27 enzymes) # ######################################### AccII AciI BcnI BsaJI BssKI BstNI BstUI CfoI Cfr13I DraII EcoO109I EcoRII Fnu4HI HaeIII HapII HhaI HinP1I HpaII ItaI MspI MvaI MvnI MwoI NciI NlaIV Sau96I ScrFI