(Linear) (Six-base) MAPSORT of: KIEE6537.seq Check: 3001 from: 1 to: 234 KIEE6537.orf With 182 enzymes: SgfI * March 16, 2015 11:33 .. AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 10 42 234 Size: 10 32 192 Fragments arranged by size: 192 32 10 ApaI G_GGCC'C Cuts at: 0 166 172 234 Size: 166 6 62 Fragments arranged by size: 166 62 6 AvaI C'yCGr_G Cuts at: 0 165 200 234 Size: 165 35 34 Fragments arranged by size: 165 35 34 BanI G'GyrC_C Cuts at: 0 73 234 Size: 73 161 BanII G_rGCy'C Cuts at: 0 166 172 234 Size: 166 6 62 Fragments arranged by size: 166 62 6 BbeI G_GCGC'C Cuts at: 0 77 234 Size: 77 157 BglI GCCn_nnn'nGGC Cuts at: 0 82 161 234 Size: 82 79 73 Fragments arranged by size: 82 79 73 Bme1580I G_kGCm'C Cuts at: 0 166 172 234 Size: 166 6 62 Fragments arranged by size: 166 62 6 BsaHI Gr'CG_yC Cuts at: 0 74 234 Size: 74 160 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 10 40 179 209 234 Size: 10 30 139 30 25 Fragments arranged by size: 139 30 30 25 10 BseYI C'CCAG_C Cuts at: 0 190 223 234 Size: 190 33 11 Fragments arranged by size: 190 33 11 BsiEI CG_ry'CG Cuts at: 0 86 156 234 Size: 86 70 78 Fragments arranged by size: 86 78 70 Bsp1286I G_dGCh'C Cuts at: 0 166 172 234 Size: 166 6 62 Fragments arranged by size: 166 62 6 BsrBI CCG'CTC Cuts at: 0 143 234 Size: 143 91 EaeI y'GGCC_r Cuts at: 0 83 153 234 Size: 83 70 81 Fragments arranged by size: 83 81 70 EagI C'GGCC_G Cuts at: 0 83 153 234 Size: 83 70 81 Fragments arranged by size: 83 81 70 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 200 234 Size: 200 34 EcoO109I rG'GnC_Cy Cuts at: 0 162 169 178 234 Size: 162 7 9 56 Fragments arranged by size: 162 56 9 7 HaeII r_GCGC'y Cuts at: 0 77 234 Size: 77 157 KasI G'GCGC_C Cuts at: 0 73 234 Size: 73 161 MspA1I CmG'CkG Cuts at: 0 60 227 234 Size: 60 167 7 Fragments arranged by size: 167 60 7 NarI GG'CG_CC Cuts at: 0 74 234 Size: 74 160 NotI GC'GGCC_GC Cuts at: 0 83 234 Size: 83 151 PasI CC'CwG_GG Cuts at: 0 172 234 Size: 172 62 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 10 42 234 Size: 10 32 192 Fragments arranged by size: 192 32 10 PspOMI G'GGCC_C Cuts at: 0 162 168 234 Size: 162 6 66 Fragments arranged by size: 162 66 6 SfiI GGCCn_nnn'nGGCC Cuts at: 0 161 234 Size: 161 73 SfoI GGC'GCC Cuts at: 0 75 234 Size: 75 159 SmaI CCC'GGG Cuts at: 0 167 202 234 Size: 167 35 32 Fragments arranged by size: 167 35 32 SrfI GCCC'GGGC Cuts at: 0 167 234 Size: 167 67 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 179 211 234 Size: 179 32 23 Fragments arranged by size: 179 32 23 XbaI T'CTAG_A Cuts at: 0 48 234 Size: 48 186 XmaI C'CCGG_G Cuts at: 0 165 200 234 Size: 165 35 34 Fragments arranged by size: 165 35 34 Enzymes that do cut: AloI ApaI AvaI BanI BanII BbeI BglI Bme1580I BsaHI BsaXI BseYI BsiEI Bsp1286I BsrBI EaeI EagI EciI EcoO109I HaeII KasI MspA1I NarI NotI PasI PpiI PspOMI SfiI SfoI SmaI SrfI TstI XbaI XmaI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbsI BbvCI BcgI BciVI BclI BfrBI BglII BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI NaeI NcoI NdeI NgoMIV NheI NruI NsiI NspI PacI PciI PflMI PfoI PmeI PmlI PpuMI PshAI PsiI PspXI PsrI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmlI SnaBI SpeI SphI SspI StuI StyI SwaI TaqII TatI Tth111I XcmI XhoI XmnI ZraI