################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AfaI AflII AflIII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BsaAI BsaBI BsaHI BsaI BseAI BsgI BsiEI BsiHKAI BsiWI BsiYI BslI BsmBI BsoBI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrDI BsrFI BsrGI BsrI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstNI BstPI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I Cfr13I ClaI CpoI DraI DraII DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI FbaI FokI FseI FspI HaeII HaeIII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI KasI KpnI KspI MaeII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspA1I MunI MvaI NaeI NarI NcoI NdeI NgoMIV NheI NlaIV NotI NruI NspI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshBI Psp1406I PstI PvuI PvuII RcaI RsaI RsrII SacI SacII SalI SapI Sau96I SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI StyI SwaI Tru9I TspRI Tth111I Van91I XcmI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AccII 73 CGCG CG^CG AciI 198 CCGC CCGC(-3/-1) AcsI 32 GAATTC [GA]^AATT[CT] ApoI 32 GAATTC [GA]^AATT[CT] BcnI 375 CCGGG CC^[GC]GG BfaI 139 CTAG C^TAG BsaJI 375 CCGGGG C^C[ACGT][ACGT]GG BsaWI 93 ACCGGA [AT]^CCGG[AT] BsmFI 378 GGGAC GGGAC(10/14) BsmI 217 GCATTC GAATGC(1/-1) BssKI 375 CCGGG ^CC[ACGT]GG BstUI 73 CGCG CG^CG BstXI 169 CCAGCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG EcoRI 32 GAATTC G^AATTC EcoRV 273 GATATC GAT^ATC EcoT22I 215 ATGCAT ATGCA^T HphI 302 TCACC GGTGA(8/7) MaeI 139 CTAG C^TAG MaeIII 301 GTCAC ^GT[ACGT]AC MvnI 73 CGCG CG^CG MwoI 109 GCTTTTGCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NciI 375 CCGGG CC^[GC]GG NlaIII 214 CATG CATG^ NsiI 215 ATGCAT ATGCA^T PshAI 294 GACATCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC ScrFI 375 CCGGG CC^[ACGT]GG Tsp45I 301 GTCAC ^GT[GC]AC Tsp509I 33 AATT ^AATT XbaI 138 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 16 enzymes) # ################################### AluI 108 AGCT AG^CT 366 AGCT AlwI 166 GATCC GGATC(4/5) 250 GATCC BbsI 193 GAAGAC GAAGAC(2/6) 422 GTCTTC BpuAI 193 GAAGAC GAAGAC(2/6) 422 GTCTTC BseRI 223 GAGGAG GAGGAG(10/8) 394 GAGGAG BsmAI 292 GAGAC GTCTC(1/5) 267 GTCTC DpnII 166 GATC ^GATC 250 GATC HapII 94 CCGG C^CGG 375 CCGG HpaII 94 CCGG C^CGG 375 CCGG MboI 166 GATC ^GATC 250 GATC MspI 94 CCGG C^CGG 375 CCGG NdeII 166 GATC ^GATC 250 GATC PleI 313 GACTC GAGTC(4/5) 265 GAGTC Sau3AI 166 GATC ^GATC 250 GATC TaqI 4 TCGA T^CGA 221 TCGA TthHB8I 4 TCGA T^CGA 221 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 11 enzymes) # ######################################### BbvI DdeI EarI Fnu4HI HinfI ItaI Ksp632I MboII MnlI TfiI TseI