Restriction map for
pFN21AE6609
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# NO CUT ENZYMES ( 159 enzymes) #
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AatII Acc65I AccII AccIII AclI
AcsI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI Asp700I Asp718I
AspEI AspHI AspI AvaI AviII
BamHI BanI BanII BbeI BbrPI
BbvCI BcgI BclI BcnI BfrI
BglI BglII BmyI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmAI BsmBI
BsmI BsoBI Bsp1286I BspDI BspEI
BspMI BsrFI BssHII BssSI Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I Cac8I Cfr10I ClaI CpoI
DraII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV FauI
FbaI FseI FspI HapII HgaI
Hin1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MluI MroI MspA1I
MspI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfcI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SwaI Tsp45I Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 51 enzymes) #
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AccI 530 GTCTAC GT^[AC][GT]AC
AlwI 3 GGATC GGATC(4/5)
AvaII 790 GGACC G^G[AT]CC
AvrII 324 CCTAGG C^CTAGG
BalI 64 TGGCCA TGG^CCA
BciVI 51 GTATCC GTATCC(6/5)
BfaI 325 CTAG C^TAG
BlnI 324 CCTAGG C^CTAGG
BlpI 674 GCTCAGC GC^T[ACGT]AGC
BpmI 289 CTGGAG CTGGAG(16/14)
Bpu1102I 674 GCTCAGC GC^T[ACGT]AGC
BsaAI 46 TACGTG [CT]AC^GT[GA]
Bsp1407I 590 TGTACA T^GTACA
BspHI 624 TCATGA T^CATGA
BspLU11I 392 ACATGT A^CATGT
BsrBI 793 CCGCTC CCGCTC(-3/-3)
BsrDI 248 CATTGC GCAATG(2/0)
BsrGI 590 TGTACA T^GTACA
BssKI 243 CCAGG ^CC[ACGT]GG
BstAPI 490 GCAAGTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstNI 243 CCAGG CC^[AT]GG
BstXI 55 CCAGCCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 3 GGATCT [GA]^GATC[CT]
CelII 674 GCTCAGC GC^T[ACGT]AGC
Cfr13I 790 GGACC G^G[ACGT]CC
DraI 204 TTTAAA TTT^AAA
DsaI 253 CCATGG C^C[GA][CT]GG
EaeI 64 TGGCCA [CT]^GGCC[GA]
EcoRII 243 CCAGG ^CC[AT]GG
EcoT22I 179 ATGCAT ATGCA^T
HaeII 443 AGCGCC [GA]GCGC^[CT]
MaeI 325 CTAG C^TAG
MaeII 47 ACGT A^CGT
MflI 3 GGATCT [GA]^GATC[CT]
MluNI 64 TGGCCA TGG^CCA
MscI 64 TGGCCA TGG^CCA
MvaI 243 CCAGG CC^[AT]GG
MwoI 490 GCAAGTCATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NcoI 253 CCATGG C^CATGG
NlaIV 789 GGGACC GG[ACGT]^[ACGT]CC
NsiI 179 ATGCAT ATGCA^T
NspI 392 ACATGT [GA]CATG^[CT]
PleI 528 GAGTC GAGTC(4/5)
RcaI 624 TCATGA T^CATGA
Sau96I 790 GGACC G^G[ACGT]CC
ScrFI 243 CCAGG CC^[ACGT]GG
SspBI 590 TGTACA T^GTACA
SspI 725 AATATT AAT^ATT
StuI 417 AGGCCT AGG^CCT
XcmI 837 CCATTCAGGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 3 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AciI 793 CCGC CCGC(-3/-1)
752 GCGG
AfaI 10 GTAC GT^AC
591 GTAC
AflIII 47 ACGTGT A^C[GA][CT]GT
392 ACATGT
AseI 183 ATTAAT AT^TAAT
667 ATTAAT
AsnI 183 ATTAAT AT^TAAT
667 ATTAAT
BbsI 98 GTCTTC GAAGAC(2/6)
169 GTCTTC
BbvI 830 GCAGC GCAGC(8/12)
833 GCAGC
BpuAI 98 GTCTTC GAAGAC(2/6)
169 GTCTTC
BsaJI 253 CCATGG C^C[ACGT][ACGT]GG
324 CCTAGG
BsiYI 508 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
776 CCTTTGAGGGG
BslI 508 CCTATTGATGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
776 CCTTTGAGGGG
BsmFI 789 GGGAC GGGAC(10/14)
643 GTCCC
BsrI 404 ACTGG ACTGG(1/-1)
432 ACTGG
CfoI 444 GCGC GCG^C
828 GCGC
DpnII 4 GATC ^GATC
175 GATC
DraIII 568 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
732 CACTTTGTG
EcoT14I 253 CCATGG C^C[AT][AT]GG
324 CCTAGG
HaeIII 65 GGCC GG^CC
418 GGCC
HhaI 444 GCGC GCG^C
828 GCGC
HinP1I 444 GCGC G^CGC
828 GCGC
MaeIII 109 GTAAC ^GT[ACGT]AC
302 GTTAC
MboI 4 GATC ^GATC
175 GATC
NdeI 338 CATATG CA^TATG
697 CATATG
NdeII 4 GATC ^GATC
175 GATC
PshBI 183 ATTAAT AT^TAAT
667 ATTAAT
RsaI 10 GTAC GT^AC
591 GTAC
Sau3AI 4 GATC ^GATC
175 GATC
StyI 253 CCATGG C^C[AT][AT]GG
324 CCTAGG
TaqI 173 TCGA T^CGA
222 TCGA
TseI 830 GCAGC G^C[AT]GC
833 GCAGC
TthHB8I 173 TCGA T^CGA
222 TCGA
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# 3 OR MORE CUT ENZYMES ( 17 enzymes) #
#########################################
AluI BstF5I DdeI Fnu4HI FokI
HinfI HphI ItaI MboII MnlI
MseI MslI NlaIII TfiI Tru9I
Tsp509I TspRI