################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII AccII AclI AcsI AflII AgeI AhdI Alw44I Aor51HI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BbrPI BbsI BbvCI BclI BfrI BglI BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsgI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I CelII Cfr10I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoT22I FauI FbaI FseI FspI HaeII HincII HindII HindIII HpaI KasI KspI MamI MfeI MluI MslI MspA1I MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tsp509I Tth111I XbaI XcmI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### Acc65I 1277 GGTACC G^GTACC AccI 1051 GTCTAC GT^[AC][GT]AC AccIII 217 TCCGGA T^CCGGA AcyI 995 GACGCC G[GA]^CG[CT]C AflIII 722 ACATGT A^C[GA][CT]GT AlwNI 325 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1076 CCTCAGG CC^T[ACGT]AGG ApaI 1089 GGGCCC GGGCC^C Asp718I 1277 GGTACC G^GTACC AspHI 795 GAGCAC G[AT]GC[AT]^C AvaI 562 CTCGAG C^[CT]CG[GA]G BalI 1028 TGGCCA TGG^CCA BamHI 469 GGATCC G^GATCC BcgI 1012 CGAATCAACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 319 GTATCC GTATCC(6/5) BfaI 115 CTAG C^TAG BsaHI 995 GACGCC G[GA]^CG[CT]C BsaWI 217 TCCGGA [AT]^CCGG[AT] BseAI 217 TCCGGA T^CCGGA BsiHKAI 795 GAGCAC G[AT]GC[AT]^C BsoBI 562 CTCGAG C^[CT]CG[GA]G BspEI 217 TCCGGA T^CCGGA BspLU11I 722 ACATGT A^CATGT BspMI 204 ACCTGC ACCTGC(4/8) BsrBI 277 CCGCTC CCGCTC(-3/-3) Bsu36I 1076 CCTCAGG CC^T[ACGT]AGG CfoI 603 GCGC GCG^C Eco81I 1076 CCTCAGG CC^T[ACGT]AGG EcoNI 1180 CCTGGCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 28 GATATC GAT^ATC EcoT14I 956 CCATGG C^C[AT][AT]GG HgaI 995 GACGC GACGC(5/10) HhaI 603 GCGC GCG^C Hin1I 995 GACGCC G[GA]^CG[CT]C HinP1I 603 GCGC G^CGC KpnI 1277 GGTACC GGTAC^C MaeI 115 CTAG C^TAG MaeII 1138 ACGT A^CGT MluNI 1028 TGGCCA TGG^CCA MroI 217 TCCGGA T^CCGGA MscI 1028 TGGCCA TGG^CCA NcoI 956 CCATGG C^CATGG NspI 722 ACATGT [GA]CATG^[CT] PaeR7I 562 CTCGAG C^TCGAG PflMI 415 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 201 AGGACCT [GA]G^G[AT]CC[CT] StuI 1168 AGGCCT AGG^CCT StyI 956 CCATGG C^C[AT][AT]GG Van91I 415 CCACAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 562 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 15 enzymes) # ################################### AfaI 1118 GTAC GT^AC 1278 GTAC AvaII 202 GGACC G^G[AT]CC 854 GGACC BanI 452 GGCACC G^G[CT][GA]CC 1277 GGTACC BanII 550 GAGCCC G[GA]GC[CT]^C 1089 GGGCCC BglII 144 AGATCT A^GATCT 929 AGATCT BsmFI 429 GGGAC GGGAC(10/14) 1102 GTCCC BstAPI 285 GCAGCTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 357 GCAAGGTCTGC BstXI 225 CCAAATGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 441 CCAGGCATTTGG DsaI 59 CCACGG C^C[GA][CT]GG 956 CCATGG HphI 3 GGTGA GGTGA(8/7) 972 GGTGA PstI 160 CTGCAG CTGCA^G 364 CTGCAG RsaI 1118 GTAC GT^AC 1278 GTAC SfcI 160 CTGCAG C^T[GA][CT]AG 364 CTGCAG SmlI 562 CTCGAG C^T[CT][GA]AG 733 CTCAAG Tsp45I 152 GTGAC ^GT[GC]AC 557 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 61 enzymes) # ######################################### AciI AluI AlwI BbvI BcnI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII ItaI Ksp632I MaeIII MboI MboII MflI MnlI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI Sau3AI Sau96I ScrFI SfaNI TaqI TfiI Tru9I TseI TspRI TthHB8I XhoII