(Circular) (Six-base) MAPSORT of: pFN21AB7735.seq Check: 9415 from: 1 to: 6013 pFN21AB7735 6013 bp With 182 enzymes: SgfI * March 28, 2012 14:22 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3823 278 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3057 3356 1937 Size: 1120 299 4594 Fragments arranged by size: 4594 1120 299 Acc65I G'GTAC_C Cuts at: 3282 3334 3282 Size: 52 5961 AclI AA'CG_TT Cuts at: 1563 4140 4513 1563 Size: 2577 373 3063 Fragments arranged by size: 3063 2577 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2659 3255 4076 5460 5728 1632 Size: 1027 596 821 1384 268 1917 Fragments arranged by size: 1917 1384 1027 821 596 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5816 AflIII A'CryG_T Cuts at: 1203 2727 4912 5889 1203 Size: 1524 2185 977 1327 Fragments arranged by size: 2185 1524 1327 977 AgeI A'CCGG_T Cuts at: 4844 4844 Size: 6013 AhdI GACnn_n'nnGTC Cuts at: 4742 4742 Size: 6013 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6013 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6013 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3239 3271 3239 Size: 32 5981 AlwNI CAG_nnn'CTG Cuts at: 2335 3756 5328 2335 Size: 1421 1572 3020 Fragments arranged by size: 3020 1572 1421 ApaI G_GGCC'C Cuts at: 3098 3098 Size: 6013 ApaLI G'TGCA_C Cuts at: 4070 5226 4070 Size: 1156 4857 ApoI r'AATT_y Cuts at: 1493 3322 3548 3700 5809 1493 Size: 1829 226 152 2109 1697 Fragments arranged by size: 2109 1829 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4567 160 Size: 872 3535 1606 Fragments arranged by size: 3535 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 AvaI C'yCGr_G Cuts at: 1870 1943 2567 3338 1870 Size: 73 624 771 4545 Fragments arranged by size: 4545 771 624 73 BamHI G'GATC_C Cuts at: 2474 3343 3710 2474 Size: 869 367 4777 Fragments arranged by size: 4777 869 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2457 3282 3334 4789 618 Size: 325 217 627 670 825 52 1455 1842 Fragments arranged by size: 1842 1455 825 670 627 325 217 52 BanII G_rGCy'C Cuts at: 1391 2559 3098 3332 1391 Size: 1168 539 234 4072 Fragments arranged by size: 4072 1168 539 234 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5042 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3006 3040 4204 4238 1903 Size: 34 1069 34 1164 34 3678 Fragments arranged by size: 3678 1164 1069 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2335 3905 5115 2335 Size: 1570 1210 3233 Fragments arranged by size: 3233 1570 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 6013 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4624 136 Size: 107 122 71 631 605 2952 1525 Fragments arranged by size: 2952 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2149 2934 6008 2149 Size: 785 3074 2154 Fragments arranged by size: 3074 2154 785 BlpI GC'TnA_GC Cuts at: 3430 3430 Size: 6013 Bme1580I G_kGCm'C Cuts at: 1790 2460 2523 3098 4074 5230 1790 Size: 670 63 575 976 1156 2573 Fragments arranged by size: 2573 1156 976 670 575 63 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6013 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2323 2436 2510 Size: 416 53 83 399 24 906 113 74 Cuts at: 2510 2553 3744 4702 442 Size: 43 1191 958 1753 Fragments arranged by size: 1753 1191 958 906 416 399 113 83 74 53 43 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6013 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2953 2953 Size: 6013 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2036 2270 2562 2597 2771 2912 3241 4673 Size: 797 234 292 35 174 141 329 1432 Cuts at: 4673 1239 Size: 2579 Fragments arranged by size: 2579 1432 797 329 292 234 174 141 35 Bpu10I CC'TnA_GC Cuts at: 2714 3190 2714 Size: 476 5537 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2723 4138 5003 5301 2723 Size: 1415 865 298 3435 Fragments arranged by size: 3435 1415 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2414 2864 3093 4676 882 Size: 617 915 450 229 1583 2219 Fragments arranged by size: 2219 1583 915 617 450 229 BsaAI yAC'GTr Cuts at: 493 1204 3804 493 Size: 711 2600 2702 Fragments arranged by size: 2702 2600 711 BsaBI GATnn'nnATC Cuts at: 6007 6007 Size: 6013 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3001 3820 4202 4837 Size: 53 83 186 936 1468 819 382 635 Cuts at: 4837 275 Size: 1451 Fragments arranged by size: 1468 1451 936 819 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2222 4446 4844 5118 5265 5676 2222 Size: 2224 398 274 147 411 2559 Fragments arranged by size: 2559 2224 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2885 2915 3239 3269 1178 Size: 30 165 30 1482 30 324 30 3922 Fragments arranged by size: 3922 1482 324 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2372 2402 2405 2585 2945 1186 Size: 1186 30 3 180 360 4254 Fragments arranged by size: 4254 1186 360 180 30 3 BseYI C'CCAG_C Cuts at: 2349 2505 2534 2617 2807 2819 2921 5216 5712 Size: 156 29 83 190 12 102 2295 496 Cuts at: 5712 2349 Size: 2650 Fragments arranged by size: 2650 2295 496 190 156 102 83 29 12 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5440 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3463 4224 5252 1540 Size: 275 186 1462 761 1028 2301 Fragments arranged by size: 2301 1462 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2804 4074 4159 5230 2804 Size: 1270 85 1071 3587 Fragments arranged by size: 3587 1270 1071 85 BsmI GAATG_Cn' Cuts at: 3524 3617 3524 Size: 93 5920 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2460 2523 2559 2804 3098 3332 4074 Size: 399 670 63 36 245 294 234 742 Cuts at: 4074 4159 5230 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 670 399 294 245 234 85 63 36 BspEI T'CCGG_A Cuts at: 2222 5676 2222 Size: 3454 2559 BspHI T'CATG_A Cuts at: 3902 5632 3902 Size: 1730 4283 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2218 3368 844 Size: 1374 1150 3489 Fragments arranged by size: 3489 1374 1150 BsrBI CCG'CTC Cuts at: 1394 1823 2284 3900 1394 Size: 429 461 1616 3507 Fragments arranged by size: 3507 1616 461 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4508 4682 65 Size: 1183 3260 174 1396 Fragments arranged by size: 3260 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4657 4844 1700 Size: 110 2847 187 2869 Fragments arranged by size: 2869 2847 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6013 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6013 BssSI C'ACGA_G Cuts at: 4073 5085 4073 Size: 1012 5001 BstAPI GCAn_nnn'nTGC Cuts at: 794 2296 2368 794 Size: 1502 72 4439 Fragments arranged by size: 4439 1502 72 BstBI TT'CG_AA Cuts at: 4817 4817 Size: 6013 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6013 BstXI CCAn_nnnn'nTGG Cuts at: 2237 2453 2237 Size: 216 5797 BstYI r'GATC_y Cuts at: 1972 2149 2474 2934 3275 3343 3710 4095 4112 Size: 177 325 460 341 68 367 385 17 Cuts at: 4112 5564 5650 5662 6008 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 460 385 367 346 341 325 177 86 68 17 12 Bsu36I CC'TnA_GG Cuts at: 3082 3082 Size: 6013 BtgI C'CryG_G Cuts at: 513 1065 2004 2064 2961 513 Size: 552 939 60 897 3565 Fragments arranged by size: 3565 939 897 552 60 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2663 487 Size: 683 56 1437 3837 Fragments arranged by size: 3837 1437 683 56 BtsI GCAGTG_nn' Cuts at: 3213 3249 3529 4323 4343 3213 Size: 36 280 794 20 4883 Fragments arranged by size: 4883 794 280 36 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6013 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2026 2061 529 Size: 35 1462 35 4481 Fragments arranged by size: 4481 1462 35 35 DraI TTT'AAA Cuts at: 3317 3317 Size: 6013 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5020 809 Size: 507 3704 1802 Fragments arranged by size: 3704 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2186 2308 3033 Size: 54 1425 50 275 18 356 122 725 Cuts at: 3033 3460 4349 5978 8 Size: 427 889 1629 43 Fragments arranged by size: 1629 1425 889 725 427 356 275 122 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3460 1537 Size: 275 1648 4090 Fragments arranged by size: 4090 1648 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2840 2891 3274 3943 1353 Size: 1487 51 383 669 3423 Fragments arranged by size: 3423 1487 669 383 51 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2870 3241 4600 4974 5120 387 Size: 1064 138 1281 371 1359 374 146 1280 Fragments arranged by size: 1359 1281 1280 1064 374 371 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2036 2270 2562 2597 2659 2771 2912 Size: 393 404 234 292 35 62 112 141 Cuts at: 2912 3241 3255 4076 4673 5460 5728 1239 Size: 329 14 821 597 787 268 1524 Fragments arranged by size: 1524 821 787 597 404 393 329 292 268 234 141 112 62 35 14 EcoNI CCTnn'n_nnAGG Cuts at: 3189 3189 Size: 6013 EcoO109I rG'GnC_Cy Cuts at: 2207 2704 3095 2207 Size: 497 391 5125 Fragments arranged by size: 5125 497 391 EcoRI G'AATT_C Cuts at: 3322 3700 3322 Size: 378 5635 EcoRV GAT'ATC Cuts at: 2035 2035 Size: 6013 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2638 2638 Size: 6013 FspI TGC'GCA Cuts at: 4519 4519 Size: 6013 HaeII r_GCGC'y Cuts at: 1678 5160 1678 Size: 3482 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2759 2791 2942 2974 4654 Size: 32 135 32 1187 32 151 32 1680 Cuts at: 4654 4686 4728 4760 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1680 1187 151 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3357 3603 669 Size: 988 281 1419 246 3079 Fragments arranged by size: 3079 1419 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6013 HpaI GTT'AAC Cuts at: 3603 3603 Size: 6013 KpnI G_GTAC'C Cuts at: 3286 3338 3286 Size: 52 5961 MfeI C'AATT_G Cuts at: 3612 3612 Size: 6013 MluI A'CGCG_T Cuts at: 5889 5889 Size: 6013 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5127 5311 1305 Size: 3822 184 2007 Fragments arranged by size: 3822 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3035 10 Size: 54 1425 343 1203 2988 Fragments arranged by size: 2988 1425 1203 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3971 4330 4489 518 Size: 648 2805 359 159 2042 Fragments arranged by size: 2805 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3429 4106 5254 5499 1606 Size: 1823 677 1148 245 2120 Fragments arranged by size: 2120 1823 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 2961 513 Size: 552 939 957 3565 Fragments arranged by size: 3565 957 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6013 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6013 NotI GC'GGCC_GC Cuts at: 3460 3460 Size: 6013 NspI r_CATG'y Cuts at: 1132 1229 2731 3371 4916 1132 Size: 97 1502 640 1545 2229 Fragments arranged by size: 2229 1545 1502 640 97 PasI CC'CwG_GG Cuts at: 1348 2462 3158 1348 Size: 1114 696 4203 Fragments arranged by size: 4203 1114 696 PciI A'CATG_T Cuts at: 2727 4912 2727 Size: 2185 3828 PflMI CCAn_nnn'nTGG Cuts at: 1108 2426 1108 Size: 1318 4695 PmeI GTTT'AAAC Cuts at: 3317 3317 Size: 6013 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3239 3271 4082 4114 5595 5627 3239 Size: 32 811 32 1481 32 3625 Fragments arranged by size: 3625 1481 811 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2207 2207 Size: 6013 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6013 PsiI TTA'TAA Cuts at: 3583 3583 Size: 6013 PspOMI G'GGCC_C Cuts at: 3094 3094 Size: 6013 PspXI vC'TCGA_Gb Cuts at: 2567 2567 Size: 6013 PstI C_TGCA'G Cuts at: 830 2169 2373 3365 830 Size: 1339 204 992 3478 Fragments arranged by size: 3478 1339 992 204 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6013 SalI G'TCGA_C Cuts at: 1936 3355 1936 Size: 1419 4594 SbfI CC_TGCA'GG Cuts at: 3365 3365 Size: 6013 ScaI AGT'ACT Cuts at: 4261 5973 4261 Size: 1712 4301 SfcI C'TryA_G Cuts at: 826 1045 2165 2369 3361 4496 5177 5368 826 Size: 219 1120 204 992 1135 681 191 1471 Fragments arranged by size: 1471 1135 1120 992 681 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6013 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6013 SmaI CCC'GGG Cuts at: 1872 3340 1872 Size: 1468 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 2738 4117 5018 5280 820 Size: 197 926 624 171 1379 901 262 1553 Fragments arranged by size: 1553 1379 926 901 624 262 197 171 SnaBI TAC'GTA Cuts at: 493 3804 493 Size: 3311 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6013 SphI G_CATG'C Cuts at: 1132 3371 1132 Size: 2239 3774 SspI AAT'ATT Cuts at: 5 52 3937 5 Size: 47 3885 2081 Fragments arranged by size: 3885 2081 47 StuI AGG'CCT Cuts at: 1517 3175 1517 Size: 1658 4355 StyI C'CwwG_G Cuts at: 513 1065 2004 2961 3452 513 Size: 552 939 957 491 3074 Fragments arranged by size: 3074 957 939 552 491 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3055 4040 4057 4210 4395 4840 325 Size: 1255 1475 985 17 153 185 445 1498 Fragments arranged by size: 1498 1475 1255 985 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4259 5971 96 Size: 274 80 33 51 1443 2282 1712 138 Fragments arranged by size: 2282 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5973 6005 5973 Size: 32 5981 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6013 XbaI T'CTAG_A Cuts at: 3349 3349 Size: 6013 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2011 2011 Size: 6013 XhoI C'TCGA_G Cuts at: 1943 2567 1943 Size: 624 5389 XmaI C'CCGG_G Cuts at: 1870 3338 1870 Size: 1468 4545 XmnI GAAnn'nnTTC Cuts at: 4142 4142 Size: 6013 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3821 276 Size: 53 83 186 936 2287 2468 Fragments arranged by size: 2468 2287 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI