################################## # NO CUT ENZYMES ( 132 enzymes) # ################################## AatII Acc65I AclI AcsI AflII AflIII AgeI AhdI Alw44I ApaLI ApoI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BbeI BbrPI BbsI BbvCI BciVI BclI BfaI BfrI BglII BlnI BlpI Bpu1102I BpuAI BsaAI BsaBI BsaI BsiWI BsmAI BsmBI BsmI Bsp1407I BspDI BspHI BspLU11I BsrBI BsrGI BssSI Bst1107I BstAPI BstBI BstEII BstF5I BstPI BstZ17I CelII ClaI CpoI DraI DraIII DrdI Eam1105I EarI EcoNI EcoO65I EcoRI EcoRV EcoT22I FbaI FokI FseI FspI HincII HindII HindIII HpaI KasI KpnI Ksp632I MaeI MaeII MamI MfeI MluI MluNI MscI MseI MslI MunI NarI NdeI NheI NotI NruI NsiI NspI NspV PacI PflMI PinAI PleI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI PvuII RcaI RsrII SacI SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tsp509I Van91I XbaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AccIII 682 TCCGGA T^CCGGA AcyI 409 GACGCC G[GA]^CG[CT]C AfaI 696 GTAC GT^AC AluI 712 AGCT AG^CT AlwNI 75 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 601 CCTGAGG CC^T[ACGT]AGG Aor51HI 326 AGCGCT AGC^GCT ApaI 268 GGGCCC GGGCC^C AscI 169 GGCGCGCC GG^CGCGCC AspHI 291 GTGCTC G[AT]GC[AT]^C AspI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 516 CGAATCCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpmI 595 CTGGAG CTGGAG(16/14) BsaHI 409 GACGCC G[GA]^CG[CT]C BsaWI 682 TCCGGA [AT]^CCGG[AT] BseAI 682 TCCGGA T^CCGGA BsgI 619 GTGCAG GTGCAG(16/14) BsiHKAI 291 GTGCTC G[AT]GC[AT]^C BspEI 682 TCCGGA T^CCGGA BsrDI 522 CATTGC GCAATG(2/0) BssHII 170 GCGCGC G^CGCGC BstXI 435 CCAGGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstYI 68 AGATCC [GA]^GATC[CT] Bsu36I 601 CCTGAGG CC^T[ACGT]AGG DdeI 602 CTGAG C^T[ACGT]AG Eco47III 326 AGCGCT AGC^GCT Eco81I 601 CCTGAGG CC^T[ACGT]AGG Hin1I 409 GACGCC G[GA]^CG[CT]C HinfI 517 GAATC G^A[ACGT]TC KspI 263 CCGCGG CCGC^GG MflI 68 AGATCC [GA]^GATC[CT] MroI 682 TCCGGA T^CCGGA MspA1I 263 CCGCGG C[AC]G^C[GT]G NaeI 40 GCCGGC GCC^GGC NgoMIV 40 GCCGGC G^CCGGC PaeR7I 227 CTCGAG C^TCGAG PflFI 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 490 CTGCAG CTGCA^G RsaI 696 GTAC GT^AC SacII 263 CCGCGG CCGC^GG SfcI 490 CTGCAG C^T[GA][CT]AG SmlI 227 CTCGAG C^T[CT][GA]AG TfiI 517 GAATC G^A[AT]TC TspRI 53 CACTG CA[GC]TG[ACGT][ACGT]^ Tth111I 409 GACGCCGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 584 CCATGGTGAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 227 CTCGAG C^TCGAG XhoII 68 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AccI 448 GTCTAC GT^[AC][GT]AC 475 GTCTAC AlwI 69 GATCC GGATC(4/5) 308 GATCC BanII 179 GGGCTC G[GA]GC[CT]^C 268 GGGCCC BbvI 125 GCAGC GCAGC(8/12) 21 GCTGC BseRI 10 CTCCTC GAGGAG(10/8) 248 GAGGAG BspMI 430 ACCTGC ACCTGC(4/8) 621 GCAGGT BsrFI 40 GCCGGC [GA]^CCGG[CT] 418 ACCGGC Cfr10I 40 GCCGGC [GA]^CCGG[CT] 418 ACCGGC EagI 38 CGGCCG C^GGCCG 159 CGGCCG EclXI 38 CGGCCG C^GGCCG 159 CGGCCG Eco52I 38 CGGCCG C^GGCCG 159 CGGCCG EcoT14I 120 CCATGG C^C[AT][AT]GG 584 CCATGG HaeII 319 AGCGCC [GA]GCGC^[CT] 326 AGCGCT HgaI 358 GACGC GACGC(5/10) 409 GACGC MaeIII 415 GTCAC ^GT[ACGT]AC 635 GTGAC MboII 507 GAAGA GAAGA(8/7) 533 TCTTC NcoI 120 CCATGG C^CATGG 584 CCATGG SfaNI 647 GCATC GCATC(5/9) 659 GCATC StyI 120 CCATGG C^C[AT][AT]GG 584 CCATGG TaqI 228 TCGA T^CGA 374 TCGA TseI 21 GCTGC G^C[AT]GC 125 GCAGC Tsp45I 415 GTCAC ^GT[GC]AC 635 GTGAC TthHB8I 228 TCGA T^CGA 374 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 55 enzymes) # ######################################### AccII AciI AvaI AvaII BanI BcnI BglI BmyI BsaJI BsiEI BsiYI BslI BsmFI BsoBI Bsp1286I BsrI BssKI BstNI BstUI Cac8I CfoI Cfr13I DpnII DraII DsaI EaeI EcoO109I EcoRII FauI Fnu4HI HaeIII HapII HhaI HinP1I HpaII HphI ItaI MboI MnlI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PpuMI Sau3AI Sau96I ScrFI SfiI SmaI XmaCI XmaI