(Circular) (Six-base) MAPSORT of: pFN21AE1221.seq Check: 1218 from: 1 to: 5434 pFN21AE1221 5434 bp With 182 enzymes: SgfI * August 16, 2010 14:45 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2444 2617 2444 Size: 173 5261 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3244 278 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2454 2481 2777 1937 Size: 517 27 296 4594 Fragments arranged by size: 4594 517 296 27 Acc65I G'GTAC_C Cuts at: 2755 2755 Size: 5434 AclI AA'CG_TT Cuts at: 1563 3561 3934 1563 Size: 1998 373 3063 Fragments arranged by size: 3063 1998 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2305 3497 4881 5149 1632 Size: 673 1192 1384 268 1917 Fragments arranged by size: 1917 1384 1192 673 268 AfeI AGC'GCT Cuts at: 2333 2333 Size: 5434 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5237 AflIII A'CryG_T Cuts at: 1203 4333 5310 1203 Size: 3130 977 1327 Fragments arranged by size: 3130 1327 977 AgeI A'CCGG_T Cuts at: 4265 4265 Size: 5434 AhdI GACnn_n'nnGTC Cuts at: 4163 4163 Size: 5434 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5434 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5434 AlwNI CAG_nnn'CTG Cuts at: 2085 3177 4749 2085 Size: 1092 1572 2770 Fragments arranged by size: 2770 1572 1092 ApaI G_GGCC'C Cuts at: 2277 2277 Size: 5434 ApaLI G'TGCA_C Cuts at: 3491 4647 3491 Size: 1156 4278 ApoI r'AATT_y Cuts at: 1493 2743 2969 3121 5230 1493 Size: 1250 226 152 2109 1697 Fragments arranged by size: 2109 1697 1250 226 152 AscI GG'CGCG_CC Cuts at: 2175 2175 Size: 5434 AseI AT'TA_AT Cuts at: 160 1032 3988 160 Size: 872 2956 1606 Fragments arranged by size: 2956 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 AvaI C'yCGr_G Cuts at: 1870 1943 2089 2180 2232 2243 2578 2759 1870 Size: 73 146 91 52 11 335 181 4545 Fragments arranged by size: 4545 335 181 146 91 73 52 11 BamHI G'GATC_C Cuts at: 2764 3131 2764 Size: 367 5067 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2094 2302 2347 2443 2755 Size: 325 217 627 307 208 45 96 312 Cuts at: 2755 4210 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 627 325 312 307 217 208 96 45 BanII G_rGCy'C Cuts at: 1391 2188 2277 2753 1391 Size: 797 89 476 4072 Fragments arranged by size: 4072 797 476 89 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4463 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2510 2544 3625 3659 1903 Size: 34 573 34 1081 34 3678 Fragments arranged by size: 3678 1081 573 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3326 4536 3326 Size: 1210 4224 BclI T'GATC_A Cuts at: 1548 1548 Size: 5434 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2109 2127 2154 Size: 107 122 71 631 605 437 18 27 Cuts at: 2154 2163 2172 2281 4045 136 Size: 9 9 109 1764 1525 Fragments arranged by size: 1764 1525 631 605 437 122 109 107 71 27 18 9 9 BglII A'GATC_T Cuts at: 5429 5429 Size: 5434 BlpI GC'TnA_GC Cuts at: 2851 2851 Size: 5434 Bme1580I G_kGCm'C Cuts at: 1790 2097 2277 3495 4651 1790 Size: 307 180 1218 1156 2573 Fragments arranged by size: 2573 1218 1156 307 180 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5434 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2717 3165 4123 Size: 416 53 83 399 24 1300 448 958 Cuts at: 4123 442 Size: 1753 Fragments arranged by size: 1753 1300 958 448 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5434 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 4094 1239 Size: 1382 1473 2579 Fragments arranged by size: 2579 1473 1382 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3559 4424 4722 3559 Size: 865 298 4271 Fragments arranged by size: 4271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4097 882 Size: 617 2598 2219 Fragments arranged by size: 2598 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3225 493 Size: 711 2021 2702 Fragments arranged by size: 2702 2021 711 BsaBI GATnn'nnATC Cuts at: 5428 5428 Size: 5434 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2415 3241 3623 4258 Size: 53 83 186 936 882 826 382 635 Cuts at: 4258 275 Size: 1451 Fragments arranged by size: 1451 936 882 826 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2687 3867 4265 4539 4686 5097 2687 Size: 1180 398 274 147 411 3024 Fragments arranged by size: 3024 1180 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5209 Fragments arranged by size: 5209 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2006 2268 1186 Size: 820 262 4352 Fragments arranged by size: 4352 820 262 BseYI C'CCAG_C Cuts at: 2078 4637 5133 2078 Size: 2559 496 2379 Fragments arranged by size: 2559 2379 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2645 1163 Size: 573 909 3952 Fragments arranged by size: 3952 909 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2046 2167 2383 2884 3645 4673 Size: 275 186 45 121 216 501 761 1028 Cuts at: 4673 1540 Size: 2301 Fragments arranged by size: 2301 1028 761 501 275 216 186 121 45 BsiHKAI G_wGCw'C Cuts at: 2300 3495 3580 4651 2300 Size: 1195 85 1071 3083 Fragments arranged by size: 3083 1195 1071 85 BsmI GAATG_Cn' Cuts at: 2945 3038 2945 Size: 93 5341 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2097 2188 2277 2300 2753 3495 3580 Size: 399 307 91 89 23 453 742 85 Cuts at: 3580 4651 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 453 399 307 91 89 85 23 BspEI T'CCGG_A Cuts at: 2687 5097 2687 Size: 2410 3024 BspHI T'CATG_A Cuts at: 3323 5053 3323 Size: 1730 3704 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2444 2617 2789 844 Size: 1600 173 172 3489 Fragments arranged by size: 3489 1600 173 172 BsrBI CCG'CTC Cuts at: 1394 1823 3321 1394 Size: 429 1498 3507 Fragments arranged by size: 3507 1498 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2526 3929 4103 65 Size: 1183 1278 1403 174 1396 Fragments arranged by size: 1403 1396 1278 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2045 2423 4078 4265 1700 Size: 110 235 378 1655 187 2869 Fragments arranged by size: 2869 1655 378 235 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5434 BssHII G'CGCG_C Cuts at: 1925 2175 1925 Size: 250 5184 BssSI C'ACGA_G Cuts at: 3494 4506 3494 Size: 1012 4422 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5434 BstBI TT'CG_AA Cuts at: 4238 4238 Size: 5434 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5434 BstXI CCAn_nnnn'nTGG Cuts at: 2447 2447 Size: 5434 BstYI r'GATC_y Cuts at: 1972 2073 2764 3131 3516 3533 4985 5071 5083 Size: 101 691 367 385 17 1452 86 12 Cuts at: 5083 5429 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 691 385 367 346 101 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2607 2607 Size: 5434 BtgI C'CryG_G Cuts at: 513 1065 2125 2268 2589 513 Size: 552 1060 143 321 3358 Fragments arranged by size: 3358 1060 552 321 143 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2166 487 Size: 683 56 940 3755 Fragments arranged by size: 3755 940 683 56 BtsI GCAGTG_nn' Cuts at: 2950 3744 3764 2950 Size: 794 20 4620 Fragments arranged by size: 4620 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5434 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5399 DraI TTT'AAA Cuts at: 2738 2738 Size: 5434 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4441 809 Size: 507 3125 1802 Fragments arranged by size: 3125 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2043 2155 2164 Size: 54 1425 50 275 18 213 112 9 Cuts at: 2164 2881 3770 5399 8 Size: 717 889 1629 43 Fragments arranged by size: 1629 1425 889 717 275 213 112 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 2043 2164 2881 1537 Size: 275 231 121 717 4090 Fragments arranged by size: 4090 717 275 231 121 EarI CTCTTCn'nnn_ Cuts at: 1353 3364 1353 Size: 2011 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2305 2343 4021 4395 4541 387 Size: 1064 138 716 38 1678 374 146 1280 Fragments arranged by size: 1678 1280 1064 716 374 146 138 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2305 2621 3497 4094 4881 5149 1239 Size: 393 673 316 876 597 787 268 1524 Fragments arranged by size: 1524 876 787 673 597 393 316 268 EcoO109I rG'GnC_Cy Cuts at: 2228 2239 2264 2273 2228 Size: 11 25 9 5389 Fragments arranged by size: 5389 25 11 9 EcoRI G'AATT_C Cuts at: 2743 3121 2743 Size: 378 5056 FspI TGC'GCA Cuts at: 3940 3940 Size: 5434 HaeII r_GCGC'y Cuts at: 1678 2328 2335 4581 1678 Size: 650 7 2246 2531 Fragments arranged by size: 2531 2246 650 7 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4075 4107 4149 4181 1373 Size: 32 135 32 2503 32 42 32 2626 Fragments arranged by size: 2626 2503 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2778 3024 669 Size: 988 281 840 246 3079 Fragments arranged by size: 3079 988 840 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5434 HpaI GTT'AAC Cuts at: 3024 3024 Size: 5434 KpnI G_GTAC'C Cuts at: 2759 2759 Size: 5434 MfeI C'AATT_G Cuts at: 3033 3033 Size: 5434 MluI A'CGCG_T Cuts at: 5310 5310 Size: 5434 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2730 4548 4732 1305 Size: 1425 1818 184 2007 Fragments arranged by size: 2007 1818 1425 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3612 Fragments arranged by size: 3612 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3392 3751 3910 518 Size: 648 2226 359 159 2042 Fragments arranged by size: 2226 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2270 2850 3527 4675 4920 1606 Size: 664 580 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 664 580 245 NaeI GCC'GGC Cuts at: 2047 2047 Size: 5434 NcoI C'CATG_G Cuts at: 513 1065 2125 2589 513 Size: 552 1060 464 3358 Fragments arranged by size: 3358 1060 552 464 NdeI CA'TA_TG Cuts at: 387 387 Size: 5434 NgoMIV G'CCGG_C Cuts at: 2045 2045 Size: 5434 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5434 NotI GC'GGCC_GC Cuts at: 2881 2881 Size: 5434 NspI r_CATG'y Cuts at: 1132 1229 2792 4337 1132 Size: 97 1563 1545 2229 Fragments arranged by size: 2229 1563 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5434 PciI A'CATG_T Cuts at: 4333 4333 Size: 5434 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5434 PfoI T'CCnGG_A Cuts at: 2188 2188 Size: 5434 PmeI GTTT'AAAC Cuts at: 2738 2738 Size: 5434 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3503 3535 5016 5048 3503 Size: 32 1481 32 3889 Fragments arranged by size: 3889 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2228 2239 2264 2228 Size: 11 25 5398 Fragments arranged by size: 5398 25 11 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5434 PsiI TTA'TAA Cuts at: 3004 3004 Size: 5434 PspOMI G'GGCC_C Cuts at: 2273 2273 Size: 5434 PspXI vC'TCGA_Gb Cuts at: 2232 2232 Size: 5434 PstI C_TGCA'G Cuts at: 830 2499 2786 830 Size: 1669 287 3478 Fragments arranged by size: 3478 1669 287 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5434 SacII CC_GC'GG Cuts at: 2271 2271 Size: 5434 SalI G'TCGA_C Cuts at: 1936 2776 1936 Size: 840 4594 SanDI GG'GwC_CC Cuts at: 2239 2264 2239 Size: 25 5409 SbfI CC_TGCA'GG Cuts at: 2786 2786 Size: 5434 ScaI AGT'ACT Cuts at: 3682 5394 3682 Size: 1712 3722 SfcI C'TryA_G Cuts at: 826 1045 2495 2782 3917 4598 4789 826 Size: 219 1450 287 1135 681 191 1471 Fragments arranged by size: 1471 1450 1135 681 287 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2154 2163 2281 2154 Size: 9 118 5307 Fragments arranged by size: 5307 118 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5434 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5434 SmaI CCC'GGG Cuts at: 1872 2091 2182 2245 2761 1872 Size: 219 91 63 516 4545 Fragments arranged by size: 4545 516 219 91 63 SmlI C'TyrA_G Cuts at: 820 1017 1943 2232 3538 4439 4701 820 Size: 197 926 289 1306 901 262 1553 Fragments arranged by size: 1553 1306 926 901 289 262 197 SnaBI TAC'GTA Cuts at: 493 3225 493 Size: 2732 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5434 SphI G_CATG'C Cuts at: 1132 2792 1132 Size: 1660 3774 SspI AAT'ATT Cuts at: 5 52 3358 5 Size: 47 3306 2081 Fragments arranged by size: 3306 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5434 StyI C'CwwG_G Cuts at: 513 1065 2125 2589 2873 513 Size: 552 1060 464 284 3074 Fragments arranged by size: 3074 1060 552 464 284 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2611 2621 2627 3461 3478 3631 3816 Size: 1255 1031 10 6 834 17 153 185 Cuts at: 3816 4261 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1031 834 445 185 153 17 10 6 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2700 3680 5392 Size: 274 80 33 51 1443 723 980 1712 Cuts at: 5392 96 Size: 138 Fragments arranged by size: 1712 1443 980 723 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2004 2036 2603 2635 2682 2714 5394 5426 2004 Size: 32 567 32 47 32 2680 32 2012 Fragments arranged by size: 2680 2012 567 47 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2417 1316 Size: 1101 4333 XbaI T'CTAG_A Cuts at: 2770 2770 Size: 5434 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2596 2596 Size: 5434 XhoI C'TCGA_G Cuts at: 1943 2232 1943 Size: 289 5145 XmaI C'CCGG_G Cuts at: 1870 2089 2180 2243 2759 1870 Size: 219 91 63 516 4545 Fragments arranged by size: 4545 516 219 91 63 XmnI GAAnn'nnTTC Cuts at: 3563 3563 Size: 5434 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3242 276 Size: 53 83 186 936 1708 2468 Fragments arranged by size: 2468 1708 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PsrI PvuII RsrII SacI SapI SexAI SfoI SrfI SwaI