(Linear) (Six-base) MAPSORT of: KIEE1221.seq Check: 6476 from: 1 to: 729 KIEE1221.orf With 182 enzymes: SgfI * July 15, 2010 18:35 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 439 612 729 Size: 439 173 117 Fragments arranged by size: 439 173 117 AccI GT'mk_AC Cuts at: 0 449 476 729 Size: 449 27 253 Fragments arranged by size: 449 253 27 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 729 Size: 300 429 AfeI AGC'GCT Cuts at: 0 328 729 Size: 328 401 AlwNI CAG_nnn'CTG Cuts at: 0 80 729 Size: 80 649 ApaI G_GGCC'C Cuts at: 0 272 729 Size: 272 457 AscI GG'CGCG_CC Cuts at: 0 170 729 Size: 170 559 AvaI C'yCGr_G Cuts at: 0 84 175 227 238 573 729 Size: 84 91 52 11 335 156 Fragments arranged by size: 335 156 91 84 52 11 BanI G'GyrC_C Cuts at: 0 89 297 342 438 729 Size: 89 208 45 96 291 Fragments arranged by size: 291 208 96 89 45 BanII G_rGCy'C Cuts at: 0 183 272 729 Size: 183 89 457 Fragments arranged by size: 457 183 89 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 505 539 729 Size: 505 34 190 Fragments arranged by size: 505 190 34 BglI GCCn_nnn'nGGC Cuts at: 0 104 122 149 158 167 276 729 Size: 104 18 27 9 9 109 453 Fragments arranged by size: 453 109 104 27 18 9 9 Bme1580I G_kGCm'C Cuts at: 0 92 272 729 Size: 92 180 457 Fragments arranged by size: 457 180 92 BmrI ACTGGGnnnn_n' Cuts at: 0 712 729 Size: 712 17 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 616 729 Size: 616 113 BsaHI Gr'CG_yC Cuts at: 0 410 729 Size: 410 319 BsaWI w'CCGG_w Cuts at: 0 682 729 Size: 682 47 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1 263 729 Size: 1 262 466 Fragments arranged by size: 466 262 1 BseYI C'CCAG_C Cuts at: 0 73 729 Size: 73 656 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 729 Size: 640 89 BsiEI CG_ry'CG Cuts at: 0 41 162 378 729 Size: 41 121 216 351 Fragments arranged by size: 351 216 121 41 BsiHKAI G_wGCw'C Cuts at: 0 295 729 Size: 295 434 Bsp1286I G_dGCh'C Cuts at: 0 92 183 272 295 729 Size: 92 91 89 23 434 Fragments arranged by size: 434 92 91 89 23 BspEI T'CCGG_A Cuts at: 0 682 729 Size: 682 47 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 439 612 729 Size: 439 173 117 Fragments arranged by size: 439 173 117 BsrDI GCAATG_nn' Cuts at: 0 521 729 Size: 521 208 BsrFI r'CCGG_y Cuts at: 0 40 418 729 Size: 40 378 311 Fragments arranged by size: 378 311 40 BssHII G'CGCG_C Cuts at: 0 170 729 Size: 170 559 BstXI CCAn_nnnn'nTGG Cuts at: 0 442 729 Size: 442 287 BstYI r'GATC_y Cuts at: 0 68 729 Size: 68 661 Bsu36I CC'TnA_GG Cuts at: 0 602 729 Size: 602 127 BtgI C'CryG_G Cuts at: 0 120 263 584 729 Size: 120 143 321 145 Fragments arranged by size: 321 145 143 120 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 161 729 Size: 161 568 EaeI y'GGCC_r Cuts at: 0 38 150 159 729 Size: 38 112 9 570 Fragments arranged by size: 570 112 38 9 EagI C'GGCC_G Cuts at: 0 38 159 729 Size: 38 121 570 Fragments arranged by size: 570 121 38 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 300 338 729 Size: 300 38 391 Fragments arranged by size: 391 300 38 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 300 616 729 Size: 300 316 113 Fragments arranged by size: 316 300 113 EcoO109I rG'GnC_Cy Cuts at: 0 223 234 259 268 729 Size: 223 11 25 9 461 Fragments arranged by size: 461 223 25 11 9 HaeII r_GCGC'y Cuts at: 0 323 330 729 Size: 323 7 399 Fragments arranged by size: 399 323 7 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 725 729 Size: 725 4 MspA1I CmG'CkG Cuts at: 0 265 729 Size: 265 464 NaeI GCC'GGC Cuts at: 0 42 729 Size: 42 687 NcoI C'CATG_G Cuts at: 0 120 584 729 Size: 120 464 145 Fragments arranged by size: 464 145 120 NgoMIV G'CCGG_C Cuts at: 0 40 729 Size: 40 689 PfoI T'CCnGG_A Cuts at: 0 183 729 Size: 183 546 PpuMI rG'GwC_Cy Cuts at: 0 223 234 259 729 Size: 223 11 25 470 Fragments arranged by size: 470 223 25 11 PspOMI G'GGCC_C Cuts at: 0 268 729 Size: 268 461 PspXI vC'TCGA_Gb Cuts at: 0 227 729 Size: 227 502 PstI C_TGCA'G Cuts at: 0 494 729 Size: 494 235 SacII CC_GC'GG Cuts at: 0 266 729 Size: 266 463 SanDI GG'GwC_CC Cuts at: 0 234 259 729 Size: 234 25 470 Fragments arranged by size: 470 234 25 SfcI C'TryA_G Cuts at: 0 490 729 Size: 490 239 SfiI GGCCn_nnn'nGGCC Cuts at: 0 149 158 276 729 Size: 149 9 118 453 Fragments arranged by size: 453 149 118 9 SmaI CCC'GGG Cuts at: 0 86 177 240 729 Size: 86 91 63 489 Fragments arranged by size: 489 91 86 63 SmlI C'TyrA_G Cuts at: 0 227 729 Size: 227 502 StyI C'CwwG_G Cuts at: 0 120 584 729 Size: 120 464 145 Fragments arranged by size: 464 145 120 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 606 616 622 729 Size: 606 10 6 107 Fragments arranged by size: 606 107 10 6 TatI w'GTAC_w Cuts at: 0 695 729 Size: 695 34 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 31 598 630 677 709 729 Size: 31 567 32 47 32 20 Fragments arranged by size: 567 47 32 32 31 20 Tth111I GACn'n_nGTC Cuts at: 0 412 729 Size: 412 317 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 591 729 Size: 591 138 XhoI C'TCGA_G Cuts at: 0 227 729 Size: 227 502 XmaI C'CCGG_G Cuts at: 0 84 175 238 729 Size: 84 91 63 491 Fragments arranged by size: 491 91 84 63 Enzymes that do cut: AarI AccI AcuI AfeI AlwNI ApaI AscI AvaI BanI BanII BcgI BglI Bme1580I BmrI BpmI BsaHI BsaWI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrDI BsrFI BssHII BstXI BstYI Bsu36I BtgI BtgZI EaeI EagI EciI Eco57MI EcoO109I HaeII MmeI MspA1I NaeI NcoI NgoMIV PfoI PpuMI PspOMI PspXI PstI SacII SanDI SfcI SfiI SmaI SmlI StyI TaqII TatI TstI Tth111I XcmI XhoI XmaI Enzymes that do not cut: AatII Acc65I AclI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI ApoI AseI AsiSI AvrII BaeI BamHI BbeI BbsI BbvCI BciVI BclI BfrBI BglII BlpI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BsaBI BsaXI BsiWI BsmI BsmBI BspHI BsrBI BsrGI BssSI BstAPI BstBI BstEII BstZ17I BtsI ClaI CspCI DraI DraIII DrdI EarI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspI FspAI Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MscI MslI NarI NdeI NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PsrI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SexAI SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI XbaI XmnI ZraI