################################## # NO CUT ENZYMES ( 135 enzymes) # ################################## AatII AccI AccIII AclI AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I AspEI AspHI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BfrI BglI BglII BlnI BsaAI BsaBI BsaHI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrFI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI HaeII HgaI Hin1I HincII HindII HindIII HpaI KasI KspI MaeII MamI MfeI MluI MroI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tsp45I Tth111I TthHB8I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 70 GGTACC G^GTACC AccII 64 CGCG CG^CG AcsI 382 AAATTT [GA]^AATT[CT] AlwNI 610 CAGATCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 976 GGGCCC GGGCC^C ApoI 382 AAATTT [GA]^AATT[CT] Asp718I 70 GGTACC G^GTACC AvaI 721 CCCGAG C^[CT]CG[GA]G BalI 1013 TGGCCA TGG^CCA BanII 976 GGGCCC G[GA]GC[CT]^C BbsI 393 GAAGAC GAAGAC(2/6) BlpI 1026 GCTGAGC GC^T[ACGT]AGC BmyI 976 GGGCCC G[AGT]GC[ACT]^C Bpu1102I 1026 GCTGAGC GC^T[ACGT]AGC BpuAI 393 GAAGAC GAAGAC(2/6) BsaI 877 GAGACC GGTCTC(1/5) BseRI 839 GAGGAG GAGGAG(10/8) BsmI 628 GAATGC GAATGC(1/-1) BsoBI 721 CCCGAG C^[CT]CG[GA]G Bsp1286I 976 GGGCCC G[AGT]GC[ACT]^C BspHI 357 TCATGA T^CATGA BspMI 296 ACCTGC ACCTGC(4/8) BssSI 971 CACGAG CACGAG(-5/-1) BstAPI 609 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 64 CGCG CG^CG BstXI 554 CCATCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG CelII 1026 GCTGAGC GC^T[ACGT]AGC CfoI 582 GCGC GCG^C EaeI 1013 TGGCCA [CT]^GGCC[GA] EcoNI 755 CCTTCCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 630 ATGCAT ATGCA^T HhaI 582 GCGC GCG^C HinP1I 582 GCGC G^CGC KpnI 70 GGTACC GGTAC^C MluNI 1013 TGGCCA TGG^CCA MscI 1013 TGGCCA TGG^CCA MseI 1070 TTAA T^TAA MvnI 64 CGCG CG^CG NsiI 630 ATGCAT ATGCA^T PflMI 993 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 293 AGGACCT [GA]G^G[AT]CC[CT] RcaI 357 TCATGA T^CATGA SapI 446 GAAGAGC GCTCTTC(1/4) SexAI 272 ACCTGGT A^CC[AT]GGT Tru9I 1070 TTAA T^TAA Tsp509I 383 AATT ^AATT Van91I 993 CCAGAACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 852 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### BanI 70 GGTACC G^G[CT][GA]CC 764 GGCACC BcnI 515 CCCGG CC^[GC]GG 600 CCGGG BfaI 400 CTAG C^TAG 853 CTAG BsmAI 724 GAGAC GTCTC(1/5) 877 GAGAC BsmFI 179 GGGAC GGGAC(10/14) 901 GTCCC BsrDI 159 CATTGC GCAATG(2/0) 14 GCAATG BstYI 611 AGATCC [GA]^GATC[CT] 696 GGATCT EarI 417 GAAGAG CTCTTC(1/4) 446 GAAGAG HapII 516 CCGG C^CGG 600 CCGG HpaII 516 CCGG C^CGG 600 CCGG HphI 323 TCACC GGTGA(8/7) 375 TCACC Ksp632I 417 GAAGAG CTCTTC(1/4) 446 GAAGAG MaeI 400 CTAG C^TAG 853 CTAG MaeIII 8 GTAAC ^GT[ACGT]AC 962 GTAAC MflI 611 AGATCC [GA]^GATC[CT] 696 GGATCT MslI 159 CATTGCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 913 CATGAAGATG MspA1I 457 CAGCTG C[AC]G^C[GT]G 569 CAGCTG MspI 516 CCGG C^CGG 600 CCGG NciI 515 CCCGG CC^[GC]GG 600 CCGGG NspI 246 ACATGC [GA]CATG^[CT] 632 GCATGT PvuII 457 CAGCTG CAG^CTG 569 CAGCTG TfiI 60 GATTC G^A[AT]TC 186 GATTC XcmI 909 CCAGCATGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1040 CCAAAAACATCCTGG XhoII 611 AGATCC [GA]^GATC[CT] 696 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 51 enzymes) # ######################################### AciI AfaI AluI AlwI AocI AvaII BbvI BpmI BsaJI BsgI BsiYI BslI BsrI BssKI BstF5I BstNI Bsu36I Cac8I Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HinfI ItaI MboI MboII MnlI MvaI MwoI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TseI TspRI