Restriction map for
pFN21AE4345
(Circular) (Six-base) MAPSORT of: pFN21AE4345.seq Check: 3716 from: 1 to: 5788
pFN21AE4345 5788 bp
With 182 enzymes: SgfI *
July 6, 2012 17:33 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3598 278
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3131 1937
Size: 1194 4594
Acc65I G'GTAC_C
Cuts at: 2075 3109 2075
Size: 1034 4754
AclI AA'CG_TT
Cuts at: 1563 3915 4288 1563
Size: 2352 373 3063
Fragments arranged by size:
3063 2352 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3851 5235 5503 1632
Size: 2219 1384 268 1917
Fragments arranged by size:
2219 1917 1384 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5591
AflIII A'CryG_T
Cuts at: 1203 4687 5664 1203
Size: 3484 977 1327
Fragments arranged by size:
3484 1327 977
AgeI A'CCGG_T
Cuts at: 4619 4619
Size: 5788
AhdI GACnn_n'nnGTC
Cuts at: 4517 4517
Size: 5788
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5788
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5788
AlwNI CAG_nnn'CTG
Cuts at: 2620 3531 5103 2620
Size: 911 1572 3305
Fragments arranged by size:
3305 1572 911
ApaI G_GGCC'C
Cuts at: 2985 2985
Size: 5788
ApaLI G'TGCA_C
Cuts at: 3845 5001 3845
Size: 1156 4632
ApoI r'AATT_y
Cuts at: 1493 2387 3097 3323 3475 5584 1493
Size: 894 710 226 152 2109 1697
Fragments arranged by size:
2109 1697 894 710 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4342 160
Size: 872 3310 1606
Fragments arranged by size:
3310 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
AvaI C'yCGr_G
Cuts at: 1870 1943 2726 3113 1870
Size: 73 783 387 4545
Fragments arranged by size:
4545 783 387 73
BamHI G'GATC_C
Cuts at: 3118 3485 3118
Size: 367 5421
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2075 2769 3109 4564 618
Size: 325 217 627 288 694 340 1455 1842
Fragments arranged by size:
1842 1455 694 627 340 325 288 217
BanII G_rGCy'C
Cuts at: 1391 2985 3107 1391
Size: 1594 122 4072
Fragments arranged by size:
4072 1594 122
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2405 928
Size: 971 506 4311
Fragments arranged by size:
4311 971 506
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3979 4013 1903
Size: 34 2042 34 3678
Fragments arranged by size:
3678 2042 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3680 4890 3680
Size: 1210 4578
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5788
BfrBI ATG'CAT
Cuts at: 2637 2637
Size: 5788
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4399 136
Size: 107 122 71 631 605 2727 1525
Fragments arranged by size:
2727 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5783 5783
Size: 5788
BlpI GC'TnA_GC
Cuts at: 3032 3205 3032
Size: 173 5615
Bme1580I G_kGCm'C
Cuts at: 1790 2985 3849 5005 1790
Size: 1195 864 1156 2573
Fragments arranged by size:
2573 1195 1156 864
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5788
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2980 3519 4477
Size: 416 53 83 399 24 1563 539 958
Cuts at: 4477 442
Size: 1753
Fragments arranged by size:
1753 1563 958 539 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5788
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2279 2507 2862 2872 4448 1239
Size: 1040 228 355 10 1576 2579
Fragments arranged by size:
2579 1576 1040 355 228 10
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3913 4778 5076 3913
Size: 865 298 4625
Fragments arranged by size:
4625 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2876 4451 882
Size: 617 1377 1575 2219
Fragments arranged by size:
2219 1575 1377 617
BsaAI yAC'GTr
Cuts at: 493 1204 3579 493
Size: 711 2375 2702
Fragments arranged by size:
2702 2375 711
BsaBI GATnn'nnATC
Cuts at: 5782 5782
Size: 5788
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3595 3977 4612 275
Size: 53 83 186 936 2062 382 635 1451
Fragments arranged by size:
2062 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 4221 4619 4893 5040 5451 4221
Size: 398 274 147 411 4558
Fragments arranged by size:
4558 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5563
Fragments arranged by size:
5563 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2859 1186
Size: 1673 4115
BseYI C'CCAG_C
Cuts at: 2460 2913 4991 5487 2460
Size: 453 2078 496 2761
Fragments arranged by size:
2761 2078 496 453
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2187 2606 2702 1163
Size: 573 451 419 96 4249
Fragments arranged by size:
4249 573 451 419 96
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3238 3999 5027 1540
Size: 275 186 1237 761 1028 2301
Fragments arranged by size:
2301 1237 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3849 3934 5005 3849
Size: 85 1071 4632
Fragments arranged by size:
4632 1071 85
BsmI GAATG_Cn'
Cuts at: 2639 3299 3392 2639
Size: 660 93 5035
Fragments arranged by size:
5035 660 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2985 3107 3849 3934 5005 1391
Size: 399 1195 122 742 85 1071 2174
Fragments arranged by size:
2174 1195 1071 742 399 122 85
BspEI T'CCGG_A
Cuts at: 5451 5451
Size: 5788
BspHI T'CATG_A
Cuts at: 2362 3677 5407 2362
Size: 1315 1730 2743
Fragments arranged by size:
2743 1730 1315
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2310 3143 844
Size: 1466 833 3489
Fragments arranged by size:
3489 1466 833
BsrBI CCG'CTC
Cuts at: 1394 1823 3675 1394
Size: 429 1852 3507
Fragments arranged by size:
3507 1852 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2026 2163 4283 4457 65
Size: 1183 778 137 2120 174 1396
Fragments arranged by size:
2120 1396 1183 778 174 137
BsrFI r'CCGG_y
Cuts at: 1700 1810 4432 4619 1700
Size: 110 2622 187 2869
Fragments arranged by size:
2869 2622 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5788
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5788
BssSI C'ACGA_G
Cuts at: 2976 3848 4860 2976
Size: 872 1012 3904
Fragments arranged by size:
3904 1012 872
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2620 794
Size: 1826 3962
BstBI TT'CG_AA
Cuts at: 4592 4592
Size: 5788
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5788
BstXI CCAn_nnnn'nTGG
Cuts at: 2566 2566
Size: 5788
BstYI r'GATC_y
Cuts at: 1972 2616 2701 3118 3485 3870 3887 5339 5425
Size: 644 85 417 367 385 17 1452 86
Cuts at: 5425 5437 5783 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 644 417 385 367 346 86
85 17 12
Bsu36I CC'TnA_GG
Cuts at: 2628 2765 2931 2628
Size: 137 166 5485
Fragments arranged by size:
5485 166 137
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5236
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2731 487
Size: 683 56 1505 3544
Fragments arranged by size:
3544 1505 683 56
BtsI GCAGTG_nn'
Cuts at: 2794 2958 3304 4098 4118 2794
Size: 164 346 794 20 4464
Fragments arranged by size:
4464 794 346 164 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5788
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5753
DraI TTT'AAA
Cuts at: 3092 3092
Size: 5788
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4795 809
Size: 507 3479 1802
Fragments arranged by size:
3479 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 3018 3235 4124
Size: 54 1425 50 275 18 1188 217 889
Cuts at: 4124 5753 8
Size: 1629 43
Fragments arranged by size:
1629 1425 1188 889 275 217 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3235 1537
Size: 275 1423 4090
Fragments arranged by size:
4090 1423 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2417 2446 3718 1353
Size: 1064 29 1272 3423
Fragments arranged by size:
3423 1272 1064 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2051 4375 4749 4895 387
Size: 1064 138 462 2324 374 146 1280
Fragments arranged by size:
2324 1280 1064 462 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2279 2507 2862 2872 3851 4448 5235
Size: 393 647 228 355 10 979 597 787
Cuts at: 5235 5503 1239
Size: 268 1524
Fragments arranged by size:
1524 979 787 647 597 393 355 268
228 10
EcoNI CCTnn'n_nnAGG
Cuts at: 2764 2764
Size: 5788
EcoO109I rG'GnC_Cy
Cuts at: 2299 2658 2757 2981 2982 2299
Size: 359 99 224 1 5105
Fragments arranged by size:
5105 359 224 99 1
EcoRI G'AATT_C
Cuts at: 3097 3475 3097
Size: 378 5410
FspI TGC'GCA
Cuts at: 4294 4294
Size: 5788
HaeII r_GCGC'y
Cuts at: 1678 4935 1678
Size: 3257 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 4429 4461 4503 4535 1373
Size: 32 135 32 2857 32 42 32 2626
Fragments arranged by size:
2857 2626 135 42 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3132 3378 669
Size: 988 281 1194 246 3079
Fragments arranged by size:
3079 1194 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5788
HpaI GTT'AAC
Cuts at: 3378 3378
Size: 5788
KpnI G_GTAC'C
Cuts at: 2079 3113 2079
Size: 1034 4754
MfeI C'AATT_G
Cuts at: 3387 3387
Size: 5788
MluI A'CGCG_T
Cuts at: 5664 5664
Size: 5788
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4902 5086 1305
Size: 3597 184 2007
Fragments arranged by size:
3597 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3020 10
Size: 54 1425 343 1188 2778
Fragments arranged by size:
2778 1425 1188 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2168 2922 3746 4105 4264 518
Size: 648 1002 754 824 359 159 2042
Fragments arranged by size:
2042 1002 824 754 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2464 2576 3204 3881 5029 5274 1606
Size: 858 112 628 677 1148 245 2120
Fragments arranged by size:
2120 1148 858 677 628 245 112
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5236
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5788
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5788
NotI GC'GGCC_GC
Cuts at: 3235 3235
Size: 5788
NsiI A_TGCA'T
Cuts at: 2639 2639
Size: 5788
NspI r_CATG'y
Cuts at: 1132 1229 2255 2641 3146 4691 1132
Size: 97 1026 386 505 1545 2229
Fragments arranged by size:
2229 1545 1026 505 386 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5788
PciI A'CATG_T
Cuts at: 4687 4687
Size: 5788
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3004 1108
Size: 1896 3892
PfoI T'CCnGG_A
Cuts at: 3054 3054
Size: 5788
PmeI GTTT'AAAC
Cuts at: 3092 3092
Size: 5788
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3857 3889 5370 5402 3857
Size: 32 1481 32 4243
Fragments arranged by size:
4243 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2299 2299
Size: 5788
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5788
PsiI TTA'TAA
Cuts at: 3358 3358
Size: 5788
PspOMI G'GGCC_C
Cuts at: 2981 2981
Size: 5788
PstI C_TGCA'G
Cuts at: 830 2154 2469 2586 2789 3140 830
Size: 1324 315 117 203 351 3478
Fragments arranged by size:
3478 1324 351 315 203 117
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5788
PvuII CAG'CTG
Cuts at: 2464 2576 2464
Size: 112 5676
SalI G'TCGA_C
Cuts at: 1936 3130 1936
Size: 1194 4594
SapI GCTCTTCn'nnn_
Cuts at: 2446 2446
Size: 5788
SbfI CC_TGCA'GG
Cuts at: 3140 3140
Size: 5788
ScaI AGT'ACT
Cuts at: 4036 5748 4036
Size: 1712 4076
SexAI A'CCwGG_T
Cuts at: 2277 2277
Size: 5788
SfcI C'TryA_G
Cuts at: 826 1045 2150 2465 2582 2785 3136 4271 4952
Size: 219 1105 315 117 203 351 1135 681
Cuts at: 4952 5143 826
Size: 191 1471
Fragments arranged by size:
1471 1135 1105 681 351 315 219 203
191 117
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5788
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5788
SmaI CCC'GGG
Cuts at: 1872 3115 1872
Size: 1243 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3892 4793 5055 820
Size: 197 926 1949 901 262 1553
Fragments arranged by size:
1949 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3579 493
Size: 3086 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5788
SphI G_CATG'C
Cuts at: 1132 3146 1132
Size: 2014 3774
SspI AAT'ATT
Cuts at: 5 52 3712 5
Size: 47 3660 2081
Fragments arranged by size:
3660 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 5788
StyI C'CwwG_G
Cuts at: 513 1065 2238 2661 2682 3227 513
Size: 552 1173 423 21 545 3074
Fragments arranged by size:
3074 1173 552 545 423 21
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2397 2699 3815 3832 3985 4170 4615
Size: 1255 817 302 1116 17 153 185 445
Cuts at: 4615 325
Size: 1498
Fragments arranged by size:
1498 1255 1116 817 445 302 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4034 5746 96
Size: 274 80 33 51 1443 2057 1712 138
Fragments arranged by size:
2057 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2299 2331 5748 5780 2299
Size: 32 3417 32 2307
Fragments arranged by size:
3417 2307 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5788
XbaI T'CTAG_A
Cuts at: 2857 3124 2857
Size: 267 5521
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2921 3052 2921
Size: 131 5657
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5788
XmaI C'CCGG_G
Cuts at: 1870 3113 1870
Size: 1243 4545
XmnI GAAnn'nnTTC
Cuts at: 3917 3917
Size: 5788
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3596 276
Size: 53 83 186 936 2062 2468
Fragments arranged by size:
2468 2062 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI
BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI
EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NcoI NdeI NheI NotI NsiI
NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI
PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI
SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SspI StuI StyI TaqII TatI
TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI
BplI Bpu10I BsiWI BsmBI BstZ17I DraIII EcoICRI EcoRV
FalI FseI FspAI KasI NaeI NarI NgoMIV NruI
PacI PmlI PspXI PsrI RsrII SacI SacII SanDI
SfiI SfoI SrfI SwaI