(Linear) (Six-base) MAPSORT of: KIEE4345.seq Check: 4852 from: 1 to: 1083 KIEE4345.orf With 182 enzymes: SgfI * July 3, 2012 19:00 .. Acc65I G'GTAC_C Cuts at: 0 70 1083 Size: 70 1013 AlwNI CAG_nnn'CTG Cuts at: 0 615 1083 Size: 615 468 ApaI G_GGCC'C Cuts at: 0 980 1083 Size: 980 103 ApoI r'AATT_y Cuts at: 0 382 1083 Size: 382 701 AvaI C'yCGr_G Cuts at: 0 721 1083 Size: 721 362 BanI G'GyrC_C Cuts at: 0 70 764 1083 Size: 70 694 319 Fragments arranged by size: 694 319 70 BanII G_rGCy'C Cuts at: 0 980 1083 Size: 980 103 BbsI GAAGACnn'nnnn_ Cuts at: 0 400 1083 Size: 400 683 BfrBI ATG'CAT Cuts at: 0 632 1083 Size: 632 451 BlpI GC'TnA_GC Cuts at: 0 1027 1083 Size: 1027 56 Bme1580I G_kGCm'C Cuts at: 0 980 1083 Size: 980 103 BmrI ACTGGGnnnn_n' Cuts at: 0 975 1083 Size: 975 108 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 502 857 867 1083 Size: 274 228 355 10 216 Fragments arranged by size: 355 274 228 216 10 BsaI GGTCTCn'nnnn_ Cuts at: 0 871 1083 Size: 871 212 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 854 1083 Size: 854 229 BseYI C'CCAG_C Cuts at: 0 455 908 1083 Size: 455 453 175 Fragments arranged by size: 455 453 175 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 182 601 697 1083 Size: 182 419 96 386 Fragments arranged by size: 419 386 182 96 BsmI GAATG_Cn' Cuts at: 0 634 1083 Size: 634 449 Bsp1286I G_dGCh'C Cuts at: 0 980 1083 Size: 980 103 BspHI T'CATG_A Cuts at: 0 357 1083 Size: 357 726 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 305 1083 Size: 305 778 BsrDI GCAATG_nn' Cuts at: 0 21 158 1083 Size: 21 137 925 Fragments arranged by size: 925 137 21 BssSI C'ACGA_G Cuts at: 0 971 1083 Size: 971 112 BstAPI GCAn_nnn'nTGC Cuts at: 0 615 1083 Size: 615 468 BstXI CCAn_nnnn'nTGG Cuts at: 0 561 1083 Size: 561 522 BstYI r'GATC_y Cuts at: 0 611 696 1083 Size: 611 85 387 Fragments arranged by size: 611 387 85 Bsu36I CC'TnA_GG Cuts at: 0 623 760 926 1083 Size: 623 137 166 157 Fragments arranged by size: 623 166 157 137 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 726 1083 Size: 726 357 BtsI GCAGTG_nn' Cuts at: 0 789 953 1083 Size: 789 164 130 Fragments arranged by size: 789 164 130 EaeI y'GGCC_r Cuts at: 0 1013 1083 Size: 1013 70 EarI CTCTTCn'nnn_ Cuts at: 0 412 441 1083 Size: 412 29 642 Fragments arranged by size: 642 412 29 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 46 1083 Size: 46 1037 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 274 502 857 867 1083 Size: 274 228 355 10 216 Fragments arranged by size: 355 274 228 216 10 EcoNI CCTnn'n_nnAGG Cuts at: 0 759 1083 Size: 759 324 EcoO109I rG'GnC_Cy Cuts at: 0 294 653 752 976 977 1083 Size: 294 359 99 224 1 106 Fragments arranged by size: 359 294 224 106 99 1 KpnI G_GTAC'C Cuts at: 0 74 1083 Size: 74 1009 MscI TGG'CCA Cuts at: 0 1015 1083 Size: 1015 68 MslI CAynn'nnrTG Cuts at: 0 163 917 1083 Size: 163 754 166 Fragments arranged by size: 754 166 163 MspA1I CmG'CkG Cuts at: 0 459 571 1083 Size: 459 112 512 Fragments arranged by size: 512 459 112 NsiI A_TGCA'T Cuts at: 0 634 1083 Size: 634 449 NspI r_CATG'y Cuts at: 0 250 636 1083 Size: 250 386 447 Fragments arranged by size: 447 386 250 PflMI CCAn_nnn'nTGG Cuts at: 0 999 1083 Size: 999 84 PfoI T'CCnGG_A Cuts at: 0 1049 1083 Size: 1049 34 PpuMI rG'GwC_Cy Cuts at: 0 294 1083 Size: 294 789 PspOMI G'GGCC_C Cuts at: 0 976 1083 Size: 976 107 PstI C_TGCA'G Cuts at: 0 149 464 581 784 1083 Size: 149 315 117 203 299 Fragments arranged by size: 315 299 203 149 117 PvuII CAG'CTG Cuts at: 0 459 571 1083 Size: 459 112 512 Fragments arranged by size: 512 459 112 SapI GCTCTTCn'nnn_ Cuts at: 0 441 1083 Size: 441 642 SexAI A'CCwGG_T Cuts at: 0 272 1083 Size: 272 811 SfcI C'TryA_G Cuts at: 0 145 460 577 780 1083 Size: 145 315 117 203 303 Fragments arranged by size: 315 303 203 145 117 StyI C'CwwG_G Cuts at: 0 233 656 677 1083 Size: 233 423 21 406 Fragments arranged by size: 423 406 233 21 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 392 694 1083 Size: 392 302 389 Fragments arranged by size: 392 389 302 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 294 326 1083 Size: 294 32 757 Fragments arranged by size: 757 294 32 XbaI T'CTAG_A Cuts at: 0 852 1083 Size: 852 231 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 916 1047 1083 Size: 916 131 36 Fragments arranged by size: 916 131 36 Enzymes that do cut: Acc65I AlwNI ApaI ApoI AvaI BanI BanII BbsI BfrBI BlpI Bme1580I BmrI BpmI BsaI BseRI BseYI BsgI BsmI Bsp1286I BspHI BspMI BsrDI BssSI BstAPI BstXI BstYI Bsu36I BtgZI BtsI EaeI EarI EciI Eco57MI EcoNI EcoO109I KpnI MscI MslI MspA1I NsiI NspI PflMI PfoI PpuMI PspOMI PstI PvuII SapI SexAI SfcI StyI TaqII TstI XbaI XcmI Enzymes that do not cut: AarI AatII AccI AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BclI BglI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrFI BsrGI BssHII BstBI BstEII BstZ17I BtgI ClaI CspCI DraI DraIII DrdI EagI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI PacI PasI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII SalI SanDI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI TatI Tth111I XhoI XmaI XmnI ZraI