################################## # NO CUT ENZYMES ( 202 enzymes) # ################################## AatII Acc65I AccII AccIII AciI AclI AcsI AcyI AflII AflIII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AspHI AspI AviII AvrII BalI BamHI BanI BanII BbeI BbrPI BbsI BbvCI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsmI Bsp1286I Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I Cac8I CelII CfoI Cfr10I ClaI CpoI DdeI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI HhaI Hin1I HinP1I HincII HindII HindIII HpaI HpaII HphI KasI KpnI Ksp632I KspI MaeI MaeII MaeIII MamI MfeI MflI MluI MluNI MroI MscI MslI MspA1I MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tsp45I Tsp509I Tth111I Van91I XbaI XcmI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 27 enzymes) # ################################### AccI 397 GTCTAC GT^[AC][GT]AC AlwNI 451 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG AvaI 242 CTCGAG C^[CT]CG[GA]G AvaII 237 GGACC G^G[AT]CC BbvI 334 GCAGC GCAGC(8/12) BsaJI 233 CCAAGG C^C[ACGT][ACGT]GG BseRI 537 GAGGAG GAGGAG(10/8) BsiYI 27 CCTCATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BslI 27 CCTCATCCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG BsoBI 242 CTCGAG C^[CT]CG[GA]G BsrDI 405 CATTGC GCAATG(2/0) DraI 166 TTTAAA TTT^AAA EcoT14I 233 CCAAGG C^C[AT][AT]GG Fnu4HI 334 GCAGC GC^[ACGT]GC ItaI 334 GCAGC GC^[ACGT]GC MwoI 474 GCTCTACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC NlaIII 161 CATG CATG^ NlaIV 237 GGACCC GG[ACGT]^[ACGT]CC PaeR7I 242 CTCGAG C^TCGAG PleI 453 GACTC GAGTC(4/5) PpuMI 236 AGGACCC [GA]G^G[AT]CC[CT] SfaNI 139 GATGC GCATC(5/9) StyI 233 CCAAGG C^C[AT][AT]GG TfiI 216 GAATC G^A[AT]TC TseI 334 GCAGC G^C[AT]GC TspRI 256 CAGTG CA[GC]TG[ACGT][ACGT]^ XhoI 242 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 14 enzymes) # ################################### AluI 438 AGCT AG^CT 473 AGCT AlwI 265 GGATC GGATC(4/5) 492 GGATC BssKI 18 CCTGG ^CC[ACGT]GG 33 CCTGG BstNI 18 CCTGG CC^[AT]GG 33 CCTGG DraII 14 AGGGCCT [GA]G^G[ACGT]CC[CT] 236 AGGACCC EcoO109I 14 AGGGCCT [GA]G^G[ACGT]CC[CT] 236 AGGACCC EcoRII 18 CCTGG ^CC[AT]GG 33 CCTGG HaeIII 16 GGCC GG^CC 63 GGCC HinfI 216 GAATC G^A[ACGT]TC 453 GACTC MseI 92 TTAA T^TAA 167 TTAA MvaI 18 CCTGG CC^[AT]GG 33 CCTGG ScrFI 18 CCTGG CC^[ACGT]GG 33 CCTGG SmlI 242 CTCGAG C^T[CT][GA]AG 487 CTCAAG Tru9I 92 TTAA T^TAA 167 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 15 enzymes) # ######################################### AfaI BsrI BstF5I Cfr13I DpnII FokI MboI MboII MnlI NdeII RsaI Sau3AI Sau96I TaqI TthHB8I