Restriction map for
pFN21AE6189
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# NO CUT ENZYMES ( 190 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcsI AcyI AflII
AflIII AgeI AhdI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspHI AvaI AviII AvrII
BalI BanI BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BfaI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrFI BsrGI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HapII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HpaII HphI KasI KpnI
Ksp632I KspI MaeI MamI MfeI
MluI MluNI MroI MscI MseI
MslI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RcaI RsrII SacI SacII
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Van91I XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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AspI 436 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 297 GGATCC G^GATCC
BbsI 364 GTCTTC GAAGAC(2/6)
BbvI 406 GCTGC GCAGC(8/12)
BglI 304 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 364 GTCTTC GAAGAC(2/6)
BsmAI 385 GAGAC GTCTC(1/5)
BsmFI 223 GGGAC GGGAC(10/14)
BsrDI 336 CATTGC GCAATG(2/0)
BssSI 200 CACGAG CACGAG(-5/-1)
BstF5I 189 CATCC GGATG(2/0)
BstYI 297 GGATCC [GA]^GATC[CT]
Cac8I 313 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 308 CCGTGG C^C[GA][CT]GG
EcoT14I 486 CCTTGG C^C[AT][AT]GG
Fnu4HI 406 GCTGC GC^[ACGT]GC
FokI 189 CATCC GGATG(9/13)
HaeIII 25 GGCC GG^CC
ItaI 406 GCTGC GC^[ACGT]GC
MaeII 13 ACGT A^CGT
MaeIII 325 GTCAC ^GT[ACGT]AC
MboII 365 TCTTC GAAGA(8/7)
MflI 297 GGATCC [GA]^GATC[CT]
PflFI 436 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 362 GAGTC GAGTC(4/5)
SexAI 437 ACCAGGT A^CC[AT]GGT
SfcI 74 CTATAG C^T[GA][CT]AG
SmlI 85 CTTGAG C^T[CT][GA]AG
StyI 486 CCTTGG C^C[AT][AT]GG
TfiI 247 GAATC G^A[AT]TC
TseI 406 GCTGC G^C[AT]GC
Tsp45I 325 GTCAC ^GT[GC]AC
Tsp509I 106 AATT ^AATT
Tth111I 436 GACCAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 297 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 23 enzymes) #
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AfaI 31 GTAC GT^AC
477 GTAC
AlwI 298 GATCC GGATC(4/5)
297 GGATC
AvaII 174 GGACC G^G[AT]CC
195 GGACC
BanII 57 GAGCCC G[GA]GC[CT]^C
500 GGGCTC
BmyI 57 GAGCCC G[AGT]GC[ACT]^C
500 GGGCTC
BsiYI 27 CCTGGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
351 CCTTGCTTTGG
BslI 27 CCTGGTACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
351 CCTTGCTTTGG
Bsp1286I 57 GAGCCC G[AGT]GC[ACT]^C
500 GGGCTC
Cfr13I 174 GGACC G^G[ACGT]CC
195 GGACC
DdeI 156 CTCAG C^T[ACGT]AG
473 CTCAG
DpnII 298 GATC ^GATC
460 GATC
HinfI 247 GAATC G^A[ACGT]TC
362 GAGTC
MboI 298 GATC ^GATC
460 GATC
MwoI 110 GCAATACCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
304 GCCACCGTGGC
NdeII 298 GATC ^GATC
460 GATC
NlaIII 125 CATG CATG^
382 CATG
NlaIV 195 GGACCC GG[ACGT]^[ACGT]CC
297 GGATCC
RsaI 31 GTAC GT^AC
477 GTAC
Sau3AI 298 GATC ^GATC
460 GATC
Sau96I 174 GGACC G^G[ACGT]CC
195 GGACC
TaqI 270 TCGA T^CGA
462 TCGA
TspRI 149 CACTG CA[GC]TG[ACGT][ACGT]^
327 CACTG
TthHB8I 270 TCGA T^CGA
462 TCGA
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# 3 OR MORE CUT ENZYMES ( 10 enzymes) #
#########################################
AluI BsaJI BsrI BssKI BstNI
EcoRII MnlI MvaI ScrFI SfaNI