################################## # NO CUT ENZYMES ( 201 enzymes) # ################################## AatII AccI AccII AccIII AclI AcsI AcyI AflII AflIII AgeI AhdI AluI Alw44I AlwI AlwNI AocI Aor51HI ApaI ApaLI ApoI AscI AseI AsnI Asp700I AspEI AspHI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbsI BcgI BciVI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaWI BseAI BsgI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BspHI BspLU11I BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraI DraIII DrdI DsaI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FbaI FseI FspI HaeII HapII HgaI Hin1I HincII HindII HindIII HpaI HpaII HphI KasI Ksp632I KspI MaeI MaeIII MamI MfeI MflI MluI MluNI MroI MscI MseI MslI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NlaIII NotI NruI NsiI NspI NspV PacI PaeR7I PflFI PflMI PinAI PleI PmaCI PmeI PmlI PpuMI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SapI SbfI ScaI SexAI SfaNI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI TaqI Tru9I Tsp45I Tsp509I TspRI Tth111I TthHB8I Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 11 GGTACC G^GTACC AfaI 12 GTAC GT^AC Asp718I 11 GGTACC G^GTACC AvaII 42 GGACC G^G[AT]CC BanI 11 GGTACC G^G[CT][GA]CC BanII 115 GGGCTC G[GA]GC[CT]^C BbvCI 129 CCTCAGC CCTCAGC(-5/-2) BbvI 23 GCAGC GCAGC(8/12) BmyI 115 GGGCTC G[AGT]GC[ACT]^C BsaI 64 GAGACC GGTCTC(1/5) BsmAI 64 GAGAC GTCTC(1/5) BsmFI 35 GGGAC GGGAC(10/14) Bsp1286I 115 GGGCTC G[AGT]GC[ACT]^C BsrDI 74 GCAATG GCAATG(2/0) BsrI 99 ACTGG ACTGG(1/-1) BssKI 15 CCTGG ^CC[ACGT]GG BstF5I 123 CATCC GGATG(2/0) BstNI 15 CCTGG CC^[AT]GG Cac8I 167 GCAGGC GC[ACGT]^[ACGT]GC CfoI 165 GCGC GCG^C DdeI 130 CTCAG C^T[ACGT]AG DpnII 87 GATC ^GATC DraII 80 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoO109I 80 GGGGCCT [GA]G^G[ACGT]CC[CT] EcoRII 15 CCTGG ^CC[AT]GG EcoT14I 29 CCAAGG C^C[AT][AT]GG FokI 123 CATCC GGATG(9/13) HhaI 165 GCGC GCG^C HinP1I 165 GCGC G^CGC HinfI 140 GATTC G^A[ACGT]TC KpnI 11 GGTACC GGTAC^C MaeII 38 ACGT A^CGT MboI 87 GATC ^GATC MspA1I 144 CCGCTG C[AC]G^C[GT]G MvaI 15 CCTGG CC^[AT]GG NdeII 87 GATC ^GATC RsaI 12 GTAC GT^AC Sau3AI 87 GATC ^GATC ScrFI 15 CCTGG CC^[ACGT]GG StyI 29 CCAAGG C^C[AT][AT]GG TfiI 140 GATTC G^A[AT]TC TseI 23 GCAGC G^C[AT]GC XcmI 29 CCAAGGGGGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 10 enzymes) # ################################### AciI 144 CCGC CCGC(-3/-1) 20 GCGG BsaJI 15 CCTGGG C^C[ACGT][ACGT]GG 29 CCAAGG BseRI 118 CTCCTC GAGGAG(10/8) 127 CTCCTC BsiYI 27 CCCCAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCGCTGTGGGG BslI 27 CCCCAAGGGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG 144 CCGCTGTGGGG Cfr13I 42 GGACC G^G[ACGT]CC 81 GGGCC Fnu4HI 20 GCGGC GC^[ACGT]GC 23 GCAGC HaeIII 82 GGCC GG^CC 102 GGCC ItaI 20 GCGGC GC^[ACGT]GC 23 GCAGC Sau96I 42 GGACC G^G[ACGT]CC 81 GGGCC ######################################### # 3 OR MORE CUT ENZYMES ( 4 enzymes) # ######################################### MboII MnlI MwoI NlaIV