Restriction map for
pFN21AE3442
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# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AccI AccIII AclI AflII
AhdI AocI Aor51HI AscI AseI
AsnI AspEI AspI AviII AvrII
BamHI BbeI BbrPI BcgI BfrI
BlnI BsaI BseAI BsiWI BspDI
BspEI BspLU11I BsrDI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
Eam1105I Eco47III Eco81I EcoRI EcoRV
FspI HindIII HpaI KasI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SalI
ScaI SfoI SfuI SgrAI SnaBI
SpeI SwaI Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AcyI 961 GACGCC G[GA]^CG[CT]C
AflIII 3892 ACGTGT A^C[GA][CT]GT
AgeI 3388 ACCGGT A^CCGGT
Alw44I 2801 GTGCAC G^TGCAC
ApaLI 2801 GTGCAC G^TGCAC
BciVI 3449 GGATAC GTATCC(6/5)
BglI 1631 GCCCCCTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaBI 589 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 961 GACGCC G[GA]^CG[CT]C
BsaWI 3388 ACCGGT [AT]^CCGG[AT]
BsmBI 1177 CGTCTC CGTCTC(1/5)
Bsp1407I 3675 TGTACA T^GTACA
BspHI 3831 TCATGA T^CATGA
BsrBI 18 GAGCGG CCGCTC(-3/-3)
BsrGI 3675 TGTACA T^GTACA
BstEII 2418 GGTCACC G^GT[ACGT]ACC
BstPI 2418 GGTCACC G^GT[ACGT]ACC
BstXI 2543 CCAACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 1086 GACACTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 131 CGGCCG C^GGCCG
EclXI 131 CGGCCG C^GGCCG
Eco52I 131 CGGCCG C^GGCCG
EcoO65I 2418 GGTCACC G^GT[ACGT]ACC
EcoT22I 3268 ATGCAT ATGCA^T
FseI 132 GGCCGGCC GGCCGG^CC
Hin1I 961 GACGCC G[GA]^CG[CT]C
MamI 589 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NsiI 3268 ATGCAT ATGCA^T
PinAI 3388 ACCGGT A^CCGGT
RcaI 3831 TCATGA T^CATGA
SapI 1354 GAAGAGC GCTCTTC(1/4)
SbfI 1497 CCTGCAGG CCTGCA^GG
SfiI 1630 GGCCCCCTTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1497 CCTGCAGG CCTGCA^GG
SspBI 3675 TGTACA T^GTACA
SspI 447 AATATT AAT^ATT
StuI 1898 AGGCCT AGG^CCT
XcmI 1055 CCATGGAGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 35 enzymes) #
###################################
Acc65I 2892 GGTACC G^GTACC
3587 GGTACC
Asp700I 324 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2631 GAATCGGTTC
Asp718I 2892 GGTACC G^GTACC
3587 GGTACC
BalI 1052 TGGCCA TGG^CCA
2552 TGGCCA
BbvCI 2101 CCTCAGC CCTCAGC(-5/-2)
2559 CCTCAGC
BclI 1116 TGATCA T^GATCA
1289 TGATCA
BglII 929 AGATCT A^GATCT
1443 AGATCT
BlpI 2451 GCTCAGC GC^T[ACGT]AGC
3912 GCTCAGC
Bpu1102I 2451 GCTCAGC GC^T[ACGT]AGC
3912 GCTCAGC
BsaAI 760 TACGTG [CT]AC^GT[GA]
3891 TACGTG
BsiEI 131 CGGCCG CG[GA][CT]^CG
204 CGACCG
BssSI 3297 CTCGTG CACGAG(-5/-1)
3706 CTCGTG
CelII 2451 GCTCAGC GC^T[ACGT]AGC
3912 GCTCAGC
DraI 564 TTTAAA TTT^AAA
3867 TTTAAA
DraIII 457 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1402 CACACAGTG
FbaI 1116 TGATCA T^GATCA
1289 TGATCA
HincII 1477 GTCAAC GT[CT]^[GA]AC
2443 GTTGAC
HindII 1477 GTCAAC GT[CT]^[GA]AC
2443 GTTGAC
KpnI 2892 GGTACC GGTAC^C
3587 GGTACC
KspI 65 CCGCGG CCGC^GG
165 CCGCGG
MluNI 1052 TGGCCA TGG^CCA
2552 TGGCCA
MscI 1052 TGGCCA TGG^CCA
2552 TGGCCA
NaeI 133 GCCGGC GCC^GGC
3258 GCCGGC
NdeI 886 CATATG CA^TATG
2032 CATATG
NgoMIV 133 GCCGGC G^CCGGC
3258 GCCGGC
SacI 1770 GAGCTC GAGCT^C
1806 GAGCTC
SacII 65 CCGCGG CCGC^GG
165 CCGCGG
SmaI 2696 CCCGGG CCC^GGG
3626 CCCGGG
SmlI 1570 CTTGAG C^T[CT][GA]AG
3381 CTTGAG
SphI 1196 GCATGC GCATG^C
1677 GCATGC
TaqI 203 TCGA T^CGA
1152 TCGA
TthHB8I 203 TCGA T^CGA
1152 TCGA
XmaCI 2696 CCCGGG C^CCGGG
3626 CCCGGG
XmaI 2696 CCCGGG C^CCGGG
3626 CCCGGG
XmnI 324 GAAGATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2631 GAATCGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII