(Linear) (Six-base) MAPSORT of: KIEE3442.seq Check: 1596 from: 1 to: 3963 KIEE3442.orf With 182 enzymes: SgfI * October 21, 2016 17:13 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 0 1491 3963 Size: 1491 2472 Acc65I G'GTAC_C Cuts at: 0 2892 3587 3963 Size: 2892 695 376 Fragments arranged by size: 2892 695 376 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 676 1388 1714 2104 2208 3924 3963 Size: 676 712 326 390 104 1716 39 Fragments arranged by size: 1716 712 676 390 326 104 39 AflIII A'CryG_T Cuts at: 0 3892 3963 Size: 3892 71 AgeI A'CCGG_T Cuts at: 0 3388 3963 Size: 3388 575 AleI CACnn'nnGTG Cuts at: 0 1124 3963 Size: 1124 2839 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 2397 2417 2612 3963 Size: 2397 20 195 1351 Fragments arranged by size: 2397 1351 195 20 AlwNI CAG_nnn'CTG Cuts at: 0 654 810 1089 1255 1689 1692 2850 3051 Size: 654 156 279 166 434 3 1158 201 Cuts at: 3051 3183 3963 Size: 132 780 Fragments arranged by size: 1158 780 654 434 279 201 166 156 132 3 ApaI G_GGCC'C Cuts at: 0 65 725 819 1633 3633 3963 Size: 65 660 94 814 2000 330 Fragments arranged by size: 2000 814 660 330 94 65 ApaLI G'TGCA_C Cuts at: 0 2801 3963 Size: 2801 1162 ApoI r'AATT_y Cuts at: 0 576 1332 2063 2078 3963 Size: 576 756 731 15 1885 Fragments arranged by size: 1885 756 731 576 15 AvaI C'yCGr_G Cuts at: 0 1202 2696 3583 3626 3963 Size: 1202 1494 887 43 337 Fragments arranged by size: 1494 1202 887 337 43 BanI G'GyrC_C Cuts at: 0 775 2570 2892 3498 3587 3963 Size: 775 1795 322 606 89 376 Fragments arranged by size: 1795 775 606 376 322 89 BanII G_rGCy'C Cuts at: 0 65 725 819 1209 1633 1774 1810 3584 Size: 65 660 94 390 424 141 36 1774 Cuts at: 3584 3633 3963 Size: 49 330 Fragments arranged by size: 1774 660 424 390 330 141 94 65 49 36 BbsI GAAGACnn'nnnn_ Cuts at: 0 664 2023 3755 3963 Size: 664 1359 1732 208 Fragments arranged by size: 1732 1359 664 208 BbvCI CC'TCA_GC Cuts at: 0 2102 2560 3963 Size: 2102 458 1403 Fragments arranged by size: 2102 1403 458 BciVI GTATCCnnnnn_n' Cuts at: 0 3443 3963 Size: 3443 520 BclI T'GATC_A Cuts at: 0 1116 1289 3963 Size: 1116 173 2674 Fragments arranged by size: 2674 1116 173 BfrBI ATG'CAT Cuts at: 0 3270 3963 Size: 3270 693 BglI GCCn_nnn'nGGC Cuts at: 0 1637 3963 Size: 1637 2326 BglII A'GATC_T Cuts at: 0 929 1443 3963 Size: 929 514 2520 Fragments arranged by size: 2520 929 514 BlpI GC'TnA_GC Cuts at: 0 2452 3913 3963 Size: 2452 1461 50 Fragments arranged by size: 2452 1461 50 Bme1580I G_kGCm'C Cuts at: 0 65 725 780 819 1633 2573 2805 3201 Size: 65 660 55 39 814 940 232 396 Cuts at: 3201 3503 3633 3963 Size: 302 130 330 Fragments arranged by size: 940 814 660 396 330 302 232 130 65 55 39 BmgBI CAC'GTC Cuts at: 0 496 2831 3963 Size: 496 2335 1132 Fragments arranged by size: 2335 1132 496 BmrI ACTGGGnnnn_n' Cuts at: 0 59 2228 2292 2504 2792 3963 Size: 59 2169 64 212 288 1171 Fragments arranged by size: 2169 1171 288 212 64 59 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 772 1788 1872 3107 3312 3963 Size: 772 1016 84 1235 205 651 Fragments arranged by size: 1235 1016 772 651 205 84 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 759 1493 2032 2053 2149 2466 3530 3619 Size: 759 734 539 21 96 317 1064 89 Cuts at: 3619 3963 Size: 344 Fragments arranged by size: 1064 759 734 539 344 317 96 89 21 Bpu10I CC'TnA_GC Cuts at: 0 2102 2560 3963 Size: 2102 458 1403 Fragments arranged by size: 2102 1403 458 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1591 3402 3963 Size: 1591 1811 561 Fragments arranged by size: 1811 1591 561 BsaAI yAC'GTr Cuts at: 0 762 3893 3963 Size: 762 3131 70 Fragments arranged by size: 3131 762 70 BsaBI GATnn'nnATC Cuts at: 0 593 3963 Size: 593 3370 BsaHI Gr'CG_yC Cuts at: 0 962 3963 Size: 962 3001 BsaWI w'CCGG_w Cuts at: 0 3388 3963 Size: 3388 575 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 1807 1837 2778 2808 3963 Size: 1807 30 941 30 1155 Fragments arranged by size: 1807 1155 941 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 1410 1673 1780 2090 2351 3301 3864 3963 Size: 1410 263 107 310 261 950 563 99 Fragments arranged by size: 1410 950 563 310 263 261 107 99 BseYI C'CCAG_C Cuts at: 0 102 150 299 1682 2021 2707 3094 3176 Size: 102 48 149 1383 339 686 387 82 Cuts at: 3176 3502 3591 3712 3963 Size: 326 89 121 251 Fragments arranged by size: 1383 686 387 339 326 251 149 121 102 89 82 48 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 671 1303 1819 1822 2627 3963 Size: 671 632 516 3 805 1336 Fragments arranged by size: 1336 805 671 632 516 3 BsiEI CG_ry'CG Cuts at: 0 134 207 3963 Size: 134 73 3756 Fragments arranged by size: 3756 134 73 BsiHKAI G_wGCw'C Cuts at: 0 1188 1774 1810 2805 3617 3839 3963 Size: 1188 586 36 995 812 222 124 Fragments arranged by size: 1188 995 812 586 222 124 36 BsmI GAATG_Cn' Cuts at: 0 1000 2928 3270 3481 3963 Size: 1000 1928 342 211 482 Fragments arranged by size: 1928 1000 482 342 211 BsmBI CGTCTCn'nnnn_ Cuts at: 0 1183 3963 Size: 1183 2780 Bsp1286I G_dGCh'C Cuts at: 0 65 725 780 819 1188 1209 1633 1774 Size: 65 660 55 39 369 21 424 141 Cuts at: 1774 1810 2573 2805 3201 3503 3584 3617 3633 Size: 36 763 232 396 302 81 33 16 Cuts at: 3633 3839 3963 Size: 206 124 Fragments arranged by size: 763 660 424 396 369 302 232 206 141 124 81 65 55 39 36 33 21 16 BspHI T'CATG_A Cuts at: 0 3831 3963 Size: 3831 132 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1240 1491 2389 2406 2880 3963 Size: 1240 251 898 17 474 1083 Fragments arranged by size: 1240 1083 898 474 251 17 BsrBI CCG'CTC Cuts at: 0 20 3963 Size: 20 3943 BsrFI r'CCGG_y Cuts at: 0 133 1868 2938 3258 3388 3963 Size: 133 1735 1070 320 130 575 Fragments arranged by size: 1735 1070 575 320 133 130 BsrGI T'GTAC_A Cuts at: 0 3675 3963 Size: 3675 288 BssSI C'ACGA_G Cuts at: 0 3297 3706 3963 Size: 3297 409 257 Fragments arranged by size: 3297 409 257 BstAPI GCAn_nnn'nTGC Cuts at: 0 810 1255 1551 2850 3963 Size: 810 445 296 1299 1113 Fragments arranged by size: 1299 1113 810 445 296 BstEII G'GTnAC_C Cuts at: 0 2418 3963 Size: 2418 1545 BstXI CCAn_nnnn'nTGG Cuts at: 0 2550 3963 Size: 2550 1413 BstYI r'GATC_y Cuts at: 0 900 929 1443 3963 Size: 900 29 514 2520 Fragments arranged by size: 2520 900 514 29 BtgI C'CryG_G Cuts at: 0 65 165 468 920 1055 1702 3240 3963 Size: 65 100 303 452 135 647 1538 723 Fragments arranged by size: 1538 723 647 452 303 135 100 65 BtsI GCAGTG_nn' Cuts at: 0 799 1087 1745 2265 2328 3563 3963 Size: 799 288 658 520 63 1235 400 Fragments arranged by size: 1235 799 658 520 400 288 63 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 0 2714 2749 3963 Size: 2714 35 1214 Fragments arranged by size: 2714 1214 35 DraI TTT'AAA Cuts at: 0 566 3869 3963 Size: 566 3303 94 Fragments arranged by size: 3303 566 94 DraIII CAC_nnn'GTG Cuts at: 0 462 1407 3963 Size: 462 945 2556 Fragments arranged by size: 2556 945 462 DrdI GACnn_nn'nnGTC Cuts at: 0 1092 3963 Size: 1092 2871 EaeI y'GGCC_r Cuts at: 0 131 135 1052 1638 2552 2940 3963 Size: 131 4 917 586 914 388 1023 Fragments arranged by size: 1023 917 914 586 388 131 4 EagI C'GGCC_G Cuts at: 0 131 3963 Size: 131 3832 EarI CTCTTCn'nnn_ Cuts at: 0 640 1349 2123 2227 3963 Size: 640 709 774 104 1736 Fragments arranged by size: 1736 774 709 640 104 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 0 74 871 1255 3963 Size: 74 797 384 2708 Fragments arranged by size: 2708 797 384 74 EcoICRI GAG'CTC Cuts at: 0 1772 1808 3963 Size: 1772 36 2155 Fragments arranged by size: 2155 1772 36 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 676 759 1388 1493 1714 2032 2053 2104 Size: 676 83 629 105 221 318 21 51 Cuts at: 2104 2149 2208 2466 3530 3619 3924 3963 Size: 45 59 258 1064 89 305 39 Fragments arranged by size: 1064 676 629 318 305 258 221 105 89 83 59 51 45 39 21 EcoNI CCTnn'n_nnAGG Cuts at: 0 1036 2726 3518 3963 Size: 1036 1690 792 445 Fragments arranged by size: 1690 1036 792 445 EcoO109I rG'GnC_Cy Cuts at: 0 98 1630 1708 2847 3402 3623 3630 3963 Size: 98 1532 78 1139 555 221 7 333 Fragments arranged by size: 1532 1139 555 333 221 98 78 7 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 243 2932 3336 3963 Size: 243 2689 404 627 Fragments arranged by size: 2689 627 404 243 FseI GG_CCGG'CC Cuts at: 0 137 3963 Size: 137 3826 HaeII r_GCGC'y Cuts at: 0 915 1701 3126 3963 Size: 915 786 1425 837 Fragments arranged by size: 1425 915 837 786 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 399 431 1652 1684 2259 2291 3394 3426 Size: 399 32 1221 32 575 32 1103 32 Cuts at: 3426 3500 3532 3963 Size: 74 32 431 Fragments arranged by size: 1221 1103 575 431 399 74 32 32 32 32 32 HincII GTy'rAC Cuts at: 0 1479 2445 3963 Size: 1479 966 1518 Fragments arranged by size: 1518 1479 966 KpnI G_GTAC'C Cuts at: 0 2896 3591 3963 Size: 2896 695 372 Fragments arranged by size: 2896 695 372 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 478 523 1695 1850 2770 3722 3963 Size: 478 45 1172 155 920 952 241 Fragments arranged by size: 1172 952 920 478 241 155 45 MscI TGG'CCA Cuts at: 0 1054 2554 3963 Size: 1054 1500 1409 Fragments arranged by size: 1500 1409 1054 MslI CAynn'nnrTG Cuts at: 0 1124 3204 3245 3458 3891 3963 Size: 1124 2080 41 213 433 72 Fragments arranged by size: 2080 1124 433 213 72 41 MspA1I CmG'CkG Cuts at: 0 67 115 167 303 523 651 1287 1614 Size: 67 48 52 136 220 128 636 327 Cuts at: 1614 1689 1950 1971 2473 2592 3681 3963 Size: 75 261 21 502 119 1089 282 Fragments arranged by size: 1089 636 502 327 282 261 220 136 128 119 75 67 52 48 21 NaeI GCC'GGC Cuts at: 0 135 3260 3963 Size: 135 3125 703 Fragments arranged by size: 3125 703 135 NcoI C'CATG_G Cuts at: 0 468 920 1055 3963 Size: 468 452 135 2908 Fragments arranged by size: 2908 468 452 135 NdeI CA'TA_TG Cuts at: 0 887 2033 3963 Size: 887 1146 1930 Fragments arranged by size: 1930 1146 887 NgoMIV G'CCGG_C Cuts at: 0 133 3258 3963 Size: 133 3125 705 Fragments arranged by size: 3125 705 133 NsiI A_TGCA'T Cuts at: 0 3272 3963 Size: 3272 691 NspI r_CATG'y Cuts at: 0 1200 1681 2132 2451 2463 3963 Size: 1200 481 451 319 12 1500 Fragments arranged by size: 1500 1200 481 451 319 12 PflMI CCAn_nnn'nTGG Cuts at: 0 393 462 790 891 1311 3444 3963 Size: 393 69 328 101 420 2133 519 Fragments arranged by size: 2133 519 420 393 328 101 69 PfoI T'CCnGG_A Cuts at: 0 332 1470 3546 3963 Size: 332 1138 2076 417 Fragments arranged by size: 2076 1138 417 332 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 2341 2373 3963 Size: 2341 32 1590 Fragments arranged by size: 2341 1590 32 PpuMI rG'GwC_Cy Cuts at: 0 98 1708 3402 3963 Size: 98 1610 1694 561 Fragments arranged by size: 1694 1610 561 98 PspOMI G'GGCC_C Cuts at: 0 61 721 815 1629 3629 3963 Size: 61 660 94 814 2000 334 Fragments arranged by size: 2000 814 660 334 94 61 PstI C_TGCA'G Cuts at: 0 806 1094 1451 1502 1955 1976 2478 3963 Size: 806 288 357 51 453 21 502 1485 Fragments arranged by size: 1485 806 502 453 357 288 51 21 PvuII CAG'CTG Cuts at: 0 303 523 651 1287 1614 1689 1950 1971 Size: 303 220 128 636 327 75 261 21 Cuts at: 1971 2473 2592 3681 3963 Size: 502 119 1089 282 Fragments arranged by size: 1089 636 502 327 303 282 261 220 128 119 75 21 SacI G_AGCT'C Cuts at: 0 1774 1810 3963 Size: 1774 36 2153 Fragments arranged by size: 2153 1774 36 SacII CC_GC'GG Cuts at: 0 68 168 3963 Size: 68 100 3795 Fragments arranged by size: 3795 100 68 SanDI GG'GwC_CC Cuts at: 0 98 1708 3402 3963 Size: 98 1610 1694 561 Fragments arranged by size: 1694 1610 561 98 SapI GCTCTTCn'nnn_ Cuts at: 0 1349 3963 Size: 1349 2614 SbfI CC_TGCA'GG Cuts at: 0 1502 3963 Size: 1502 2461 SexAI A'CCwGG_T Cuts at: 0 386 458 661 3963 Size: 386 72 203 3302 Fragments arranged by size: 3302 386 203 72 SfcI C'TryA_G Cuts at: 0 802 1090 1122 1134 1447 1498 1594 1609 Size: 802 288 32 12 313 51 96 15 Cuts at: 1609 1951 1972 2474 2715 2839 3963 Size: 342 21 502 241 124 1124 Fragments arranged by size: 1124 802 502 342 313 288 241 124 96 51 32 21 15 12 SfiI GGCCn_nnn'nGGCC Cuts at: 0 1637 3963 Size: 1637 2326 SmaI CCC'GGG Cuts at: 0 2698 3628 3963 Size: 2698 930 335 Fragments arranged by size: 2698 930 335 SmlI C'TyrA_G Cuts at: 0 1570 3381 3963 Size: 1570 1811 582 Fragments arranged by size: 1811 1570 582 SphI G_CATG'C Cuts at: 0 1200 1681 3963 Size: 1200 481 2282 Fragments arranged by size: 2282 1200 481 SspI AAT'ATT Cuts at: 0 449 3963 Size: 449 3514 StuI AGG'CCT Cuts at: 0 1900 3963 Size: 1900 2063 StyI C'CwwG_G Cuts at: 0 263 269 468 744 920 1055 1635 3069 Size: 263 6 199 276 176 135 580 1434 Cuts at: 3069 3099 3311 3963 Size: 30 212 652 Fragments arranged by size: 1434 652 580 276 263 212 199 176 135 30 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1399 3243 3854 3963 Size: 1399 1844 611 109 Fragments arranged by size: 1844 1399 611 109 TatI w'GTAC_w Cuts at: 0 1574 3343 3675 3963 Size: 1574 1769 332 288 Fragments arranged by size: 1769 1574 332 288 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 2778 2810 3283 3315 3542 3574 3963 Size: 2778 32 473 32 227 32 389 Fragments arranged by size: 2778 473 389 227 32 32 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 1062 3963 Size: 1062 2901 XmaI C'CCGG_G Cuts at: 0 2696 3626 3963 Size: 2696 930 337 Fragments arranged by size: 2696 930 337 XmnI GAAnn'nnTTC Cuts at: 0 328 2635 3963 Size: 328 2307 1328 Fragments arranged by size: 2307 1328 328 Enzymes that do cut: AarI Acc65I AcuI AflIII AgeI AleI AlfI AlwNI ApaI ApaLI ApoI AvaI BanI BanII BbsI BbvCI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspHI BspMI BsrBI BsrFI BsrGI BssSI BstAPI BstEII BstXI BstYI BtgI BtsI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI HaeII Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NsiI NspI PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI SacII SanDI SapI SbfI SexAI SfcI SfiI SmaI SmlI SphI SspI StuI StyI TaqII TatI TstI XcmI XmaI XmnI Enzymes that do not cut: AatII AccI AclI AfeI AflII AhdI AloI AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI BmtI BsaI BsiWI BspEI BsrDI BssHII BstBI BstZ17I Bsu36I BtgZI ClaI EcoRI EcoRV FspI FspAI HindIII HpaI KasI MfeI MluI NarI NheI NotI NruI PacI PasI PciI PmeI PmlI PshAI PsiI PspXI PsrI PvuI RsrII SalI ScaI SfoI SgfI SgrAI SnaBI SpeI SrfI SwaI Tth111I XbaI XhoI ZraI