################################## # NO CUT ENZYMES ( 122 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AflIII AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI AspI AviII AvrII BalI BamHI BanI BbeI BbrPI BbvCI BcgI BfrI BglI BglII BlnI BsaBI BsaHI BsaI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII Hin1I HincII HindII HpaI KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PmaCI PmeI PmlI PshAI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI Sse8387I StuI SwaI Tth111I Van91I XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AccII 165 CGCG CG^CG AgeI 265 ACCGGT A^CCGGT AocI 756 CCTTAGG CC^T[ACGT]AGG AseI 1455 ATTAAT AT^TAAT AsnI 1455 ATTAAT AT^TAAT AvaI 1663 CTCGGG C^[CT]CG[GA]G BanII 493 GAGCTC G[GA]GC[CT]^C BciVI 1348 GTATCC GTATCC(6/5) BclI 1503 TGATCA T^GATCA BlpI 403 GCTCAGC GC^T[ACGT]AGC BpmI 907 CTCCAG CTGGAG(16/14) Bpu1102I 403 GCTCAGC GC^T[ACGT]AGC BsaAI 256 CACGTA [CT]AC^GT[GA] BsaWI 265 ACCGGT [AT]^CCGG[AT] BsgI 1888 CTGCAC GTGCAG(16/14) BsmI 1759 GAATGC GAATGC(1/-1) BsoBI 1663 CTCGGG C^[CT]CG[GA]G Bsp1407I 1637 TGTACA T^GTACA BsrDI 1641 CATTGC GCAATG(2/0) BsrFI 265 ACCGGT [GA]^CCGG[CT] BsrGI 1637 TGTACA T^GTACA BssHII 164 GCGCGC G^CGCGC BstAPI 1881 GCAGGTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstUI 165 CGCG CG^CG BstXI 584 CCAGCAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 756 CCTTAGG CC^T[ACGT]AGG CelII 403 GCTCAGC GC^T[ACGT]AGC Cfr10I 265 ACCGGT [GA]^CCGG[CT] DraIII 1366 CACGAAGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 304 GACTCAGTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 756 CCTTAGG CC^T[ACGT]AGG EcoRI 142 GAATTC G^AATTC EcoT14I 528 CCTTGG C^C[AT][AT]GG FbaI 1503 TGATCA T^GATCA MaeII 257 ACGT A^CGT MslI 422 CACAGAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MvnI 165 CGCG CG^CG PinAI 265 ACCGGT A^CCGGT PshBI 1455 ATTAAT AT^TAAT PstI 1859 CTGCAG CTGCA^G SacI 493 GAGCTC GAGCT^C SapI 2309 GAAGAGC GCTCTTC(1/4) SphI 155 GCATGC GCATG^C SspBI 1637 TGTACA T^GTACA SspI 471 AATATT AAT^ATT StyI 528 CCTTGG C^C[AT][AT]GG TaqI 1048 TCGA T^CGA TthHB8I 1048 TCGA T^CGA XbaI 921 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 18 enzymes) # ################################### Asp700I 1539 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2131 GAAGAGGTTC BbsI 202 GAAGAC GAAGAC(2/6) 651 GAAGAC BpuAI 202 GAAGAC GAAGAC(2/6) 651 GAAGAC BseRI 94 GAGGAG GAGGAG(10/8) 646 GAGGAG BspMI 212 ACCTGC ACCTGC(4/8) 1881 GCAGGT CfoI 164 GCGC GCG^C 166 GCGC DraI 465 TTTAAA TTT^AAA 1140 TTTAAA FauI 87 GCGGG CCCGC(4/6) 1779 GCGGG HgaI 408 GCGTC GACGC(5/10) 1683 GCGTC HhaI 164 GCGC GCG^C 166 GCGC HinP1I 164 GCGC G^CGC 166 GCGC HindIII 718 AAGCTT A^AGCTT 2273 AAGCTT NspI 155 GCATGC [GA]CATG^[CT] 1426 ACATGC PleI 304 GACTC GAGTC(4/5) 1441 GAGTC PvuII 2246 CAGCTG CAG^CTG 2315 CAGCTG SfcI 1154 CTACAG C^T[GA][CT]AG 1859 CTGCAG Tsp45I 13 GTGAC ^GT[GC]AC 394 GTCAC XmnI 1539 GAATATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2131 GAAGAGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 69 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BbvI BcnI BfaI BmyI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI TfiI Tru9I TseI Tsp509I TspRI XhoII