(Circular) (Six-base) MAPSORT of: pFN21AE9625.seq Check: 3798 from: 1 to: 7042 pFN21AE9625 7042 bp With 182 enzymes: SgfI * March 28, 2012 12:14 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4852 278 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4385 1937 Size: 2448 4594 Acc65I G'GTAC_C Cuts at: 4363 4363 Size: 7042 AclI AA'CG_TT Cuts at: 1563 5169 5542 1563 Size: 3606 373 3063 Fragments arranged by size: 3606 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2226 2771 2895 3129 3730 4053 4128 5105 Size: 594 545 124 234 601 323 75 977 Cuts at: 5105 6489 6757 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 977 601 594 545 323 268 234 124 75 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6845 AflIII A'CryG_T Cuts at: 1203 5941 6918 1203 Size: 4738 977 1327 Fragments arranged by size: 4738 1327 977 AgeI A'CCGG_T Cuts at: 2270 5873 2270 Size: 3603 3439 AhdI GACnn_n'nnGTC Cuts at: 5771 5771 Size: 7042 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2449 1184 Size: 1265 5777 AlwNI CAG_nnn'CTG Cuts at: 2179 2416 2554 2746 3892 4785 6357 2179 Size: 237 138 192 1146 893 1572 2864 Fragments arranged by size: 2864 1572 1146 893 237 192 138 ApaLI G'TGCA_C Cuts at: 5099 6255 5099 Size: 1156 5886 ApoI r'AATT_y Cuts at: 1493 2147 2276 3113 3258 3382 4351 4577 4729 Size: 654 129 837 145 124 969 226 152 Cuts at: 4729 6838 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 969 837 654 226 152 145 129 124 AseI AT'TA_AT Cuts at: 160 1032 3461 5596 160 Size: 872 2429 2135 1606 Fragments arranged by size: 2429 2135 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 AvaI C'yCGr_G Cuts at: 1870 1943 3668 4367 1870 Size: 73 1725 699 4545 Fragments arranged by size: 4545 1725 699 73 BamHI G'GATC_C Cuts at: 4372 4739 4372 Size: 367 6675 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4363 5818 618 Size: 325 217 627 2576 1455 1842 Fragments arranged by size: 2576 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2502 4361 1391 Size: 1111 1859 4072 Fragments arranged by size: 4072 1859 1111 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2214 2663 928 Size: 971 315 449 5307 Fragments arranged by size: 5307 971 449 315 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5233 5267 1903 Size: 34 3296 34 3678 Fragments arranged by size: 3678 3296 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3364 4934 6144 3364 Size: 1570 1210 4262 Fragments arranged by size: 4262 1570 1210 BclI T'GATC_A Cuts at: 1548 3508 1548 Size: 1960 5082 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5653 136 Size: 107 122 71 631 605 3981 1525 Fragments arranged by size: 3981 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7037 7037 Size: 7042 BlpI GC'TnA_GC Cuts at: 2409 4459 2409 Size: 2050 4992 Bme1580I G_kGCm'C Cuts at: 1790 3790 5103 6259 1790 Size: 2000 1313 1156 2573 Fragments arranged by size: 2573 2000 1313 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7042 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3604 4773 5731 Size: 416 53 83 399 24 2187 1169 958 Cuts at: 5731 442 Size: 1753 Fragments arranged by size: 2187 1753 1169 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7042 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2897 5702 1239 Size: 1658 2805 2579 Fragments arranged by size: 2805 2579 1658 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 5167 6032 6330 5167 Size: 865 298 5879 Fragments arranged by size: 5879 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5705 882 Size: 617 4206 2219 Fragments arranged by size: 4206 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 4833 493 Size: 711 1059 2570 2702 Fragments arranged by size: 2702 2570 1059 711 BsaBI GATnn'nnATC Cuts at: 7036 7036 Size: 7042 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4849 5231 5866 275 Size: 53 83 186 936 3316 382 635 1451 Fragments arranged by size: 3316 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2270 5475 5873 6147 6294 6705 2270 Size: 3205 398 274 147 411 2607 Fragments arranged by size: 3205 2607 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3002 3032 1178 Size: 30 165 30 1599 30 5188 Fragments arranged by size: 5188 1599 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2114 2666 1186 Size: 928 552 5562 Fragments arranged by size: 5562 928 552 BseYI C'CCAG_C Cuts at: 6245 6741 6245 Size: 496 6546 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3878 1163 Size: 573 2142 4327 Fragments arranged by size: 4327 2142 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4492 5253 6281 1540 Size: 275 186 2491 761 1028 2301 Fragments arranged by size: 2491 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2410 2502 2913 5103 5188 6259 2410 Size: 92 411 2190 85 1071 3193 Fragments arranged by size: 3193 2190 1071 411 92 85 BsmI GAATG_Cn' Cuts at: 3770 4553 4646 3770 Size: 783 93 6166 Fragments arranged by size: 6166 783 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2410 2502 2913 3790 4361 5103 5188 Size: 399 620 92 411 877 571 742 85 Cuts at: 5188 6259 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 877 742 620 571 411 399 92 85 BspEI T'CCGG_A Cuts at: 6705 6705 Size: 7042 BspHI T'CATG_A Cuts at: 4931 6661 4931 Size: 1730 5312 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2226 3877 4397 844 Size: 1382 1651 520 3489 Fragments arranged by size: 3489 1651 1382 520 BsrBI CCG'CTC Cuts at: 1394 1823 4929 1394 Size: 429 3106 3507 Fragments arranged by size: 3507 3106 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3645 5537 5711 65 Size: 1183 2397 1892 174 1396 Fragments arranged by size: 2397 1892 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2270 5686 5873 1700 Size: 110 460 3416 187 2869 Fragments arranged by size: 3416 2869 460 187 110 BsrGI T'GTAC_A Cuts at: 96 3642 96 Size: 3546 3496 BssHII G'CGCG_C Cuts at: 1925 2169 1925 Size: 244 6798 BssSI C'ACGA_G Cuts at: 5102 6114 5102 Size: 1012 6030 BstAPI GCAn_nnn'nTGC Cuts at: 794 3892 794 Size: 3098 3944 BstBI TT'CG_AA Cuts at: 5846 5846 Size: 7042 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7042 BstXI CCAn_nnnn'nTGG Cuts at: 2596 2596 Size: 7042 BstYI r'GATC_y Cuts at: 1972 2059 2254 3730 4372 4739 5124 5141 6593 Size: 87 195 1476 642 367 385 17 1452 Cuts at: 6593 6679 6691 7037 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1476 1452 642 385 367 346 195 87 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2762 2762 Size: 7042 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 5551 Fragments arranged by size: 5551 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6303 Fragments arranged by size: 6303 683 56 BtsI GCAGTG_nn' Cuts at: 2352 3714 4558 5352 5372 2352 Size: 1362 844 794 20 4022 Fragments arranged by size: 4022 1362 844 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7042 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2341 2376 529 Size: 35 1777 35 5195 Fragments arranged by size: 5195 1777 35 35 DraI TTT'AAA Cuts at: 2472 3147 4346 2472 Size: 675 1199 5168 Fragments arranged by size: 5168 1199 675 DraIII CAC_nnn'GTG Cuts at: 3376 3376 Size: 7042 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2315 6049 809 Size: 507 999 3734 1802 Fragments arranged by size: 3734 1802 999 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4489 5378 7007 Size: 54 1425 50 275 18 2659 889 1629 Cuts at: 7007 8 Size: 43 Fragments arranged by size: 2659 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4489 1537 Size: 275 2677 4090 Fragments arranged by size: 4090 2677 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2415 2535 3084 3846 4131 4267 4309 4972 Size: 1062 120 549 762 285 136 42 663 Cuts at: 4972 1353 Size: 3423 Fragments arranged by size: 3423 1062 762 663 549 285 136 120 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2237 5629 6003 6149 387 Size: 1064 138 648 3392 374 146 1280 Fragments arranged by size: 3392 1280 1064 648 374 146 138 EcoICRI GAG'CTC Cuts at: 2500 2500 Size: 7042 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2226 2771 2895 2897 3129 3730 4053 Size: 393 594 545 124 2 232 601 323 Cuts at: 4053 4128 5105 5702 6489 6757 1239 Size: 75 977 597 787 268 1524 Fragments arranged by size: 1524 977 787 601 597 594 545 393 323 268 232 124 75 2 EcoO109I rG'GnC_Cy Cuts at: 2067 2176 4082 4241 2067 Size: 109 1906 159 4868 Fragments arranged by size: 4868 1906 159 109 EcoRI G'AATT_C Cuts at: 2147 4351 4729 2147 Size: 2204 378 4460 Fragments arranged by size: 4460 2204 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3818 3818 Size: 7042 FspI TGC'GCA Cuts at: 5548 5548 Size: 7042 HaeII r_GCGC'y Cuts at: 1678 6189 1678 Size: 4511 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2988 3002 3020 Size: 32 135 32 614 32 770 14 18 Cuts at: 3020 3034 4010 4042 5683 5715 5757 5789 1373 Size: 14 976 32 1641 32 42 32 2626 Fragments arranged by size: 2626 1641 976 770 614 135 42 32 32 32 32 32 32 18 14 14 HincII GTy'rAC Cuts at: 669 1657 1938 4386 4632 669 Size: 988 281 2448 246 3079 Fragments arranged by size: 3079 2448 988 281 246 HindIII A'AGCT_T Cuts at: 748 2723 4278 748 Size: 1975 1555 3512 Fragments arranged by size: 3512 1975 1555 HpaI GTT'AAC Cuts at: 4632 4632 Size: 7042 KpnI G_GTAC'C Cuts at: 4367 4367 Size: 7042 MfeI C'AATT_G Cuts at: 4641 4641 Size: 7042 MluI A'CGCG_T Cuts at: 6918 6918 Size: 7042 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2280 3083 6156 6340 1305 Size: 975 803 3073 184 2007 Fragments arranged by size: 3073 2007 975 803 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5220 Fragments arranged by size: 5220 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2431 5000 5359 5518 518 Size: 648 1265 2569 359 159 2042 Fragments arranged by size: 2569 2042 1265 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2770 4253 4322 4458 5135 6283 6528 1606 Size: 1164 1483 69 136 677 1148 245 2120 Fragments arranged by size: 2120 1483 1164 1148 677 245 136 69 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 5551 Fragments arranged by size: 5551 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7042 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7042 NotI GC'GGCC_GC Cuts at: 4489 4489 Size: 7042 NspI r_CATG'y Cuts at: 1132 1229 2164 3435 4400 5945 1132 Size: 97 935 1271 965 1545 2229 Fragments arranged by size: 2229 1545 1271 965 935 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7042 PciI A'CATG_T Cuts at: 5941 5941 Size: 7042 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 7042 PfoI T'CCnGG_A Cuts at: 2039 2228 2039 Size: 189 6853 PmeI GTTT'AAAC Cuts at: 4346 4346 Size: 7042 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5111 5143 6624 6656 5111 Size: 32 1481 32 5497 Fragments arranged by size: 5497 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2067 4082 4241 2067 Size: 2015 159 4868 Fragments arranged by size: 4868 2015 159 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7042 PsiI TTA'TAA Cuts at: 4612 4612 Size: 7042 PstI C_TGCA'G Cuts at: 830 3868 4394 830 Size: 3038 526 3478 Fragments arranged by size: 3478 3038 526 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7042 PvuII CAG'CTG Cuts at: 4253 4322 4253 Size: 69 6973 SacI G_AGCT'C Cuts at: 2502 2502 Size: 7042 SalI G'TCGA_C Cuts at: 1936 4384 1936 Size: 2448 4594 SanDI GG'GwC_CC Cuts at: 2067 2067 Size: 7042 SapI GCTCTTCn'nnn_ Cuts at: 4309 4309 Size: 7042 SbfI CC_TGCA'GG Cuts at: 4394 4394 Size: 7042 ScaI AGT'ACT Cuts at: 5290 7002 5290 Size: 1712 5330 SfcI C'TryA_G Cuts at: 826 1045 3159 3864 4390 5525 6206 6397 826 Size: 219 2114 705 526 1135 681 191 1471 Fragments arranged by size: 2114 1471 1135 705 681 526 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7042 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7042 SmaI CCC'GGG Cuts at: 1872 4369 1872 Size: 2497 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 5146 6047 6309 820 Size: 197 926 3203 901 262 1553 Fragments arranged by size: 3203 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4833 493 Size: 4340 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 7042 SphI G_CATG'C Cuts at: 1132 2164 4400 1132 Size: 1032 2236 3774 Fragments arranged by size: 3774 2236 1032 SspI AAT'ATT Cuts at: 5 52 2478 4966 5 Size: 47 2426 2488 2081 Fragments arranged by size: 2488 2426 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7042 StyI C'CwwG_G Cuts at: 513 1065 2004 2533 4481 513 Size: 552 939 529 1948 3074 Fragments arranged by size: 3074 1948 939 552 529 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2686 5069 5086 5239 5424 5869 325 Size: 1255 1106 2383 17 153 185 445 1498 Fragments arranged by size: 2383 1498 1255 1106 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2139 3343 3642 Size: 274 80 33 51 1443 162 1204 299 Cuts at: 3642 5288 7000 96 Size: 1646 1712 138 Fragments arranged by size: 1712 1646 1443 1204 299 274 162 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2224 2256 7002 7034 2224 Size: 32 4746 32 2232 Fragments arranged by size: 4746 2232 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7042 XbaI T'CTAG_A Cuts at: 2926 4378 2926 Size: 1452 5590 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7042 XmaI C'CCGG_G Cuts at: 1870 4367 1870 Size: 2497 4545 XmnI GAAnn'nnTTC Cuts at: 3548 4140 5171 3548 Size: 592 1031 5419 Fragments arranged by size: 5419 1031 592 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4850 276 Size: 53 83 186 936 3316 2468 Fragments arranged by size: 3316 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SexAI SfiI SfoI SrfI SwaI XcmI