Restriction map for
pFN21AE9625
(Linear) (Six-base) MAPSORT of: KIEE9625.seq Check: 3414 from: 1 to: 2337
KIEE9625.orf
With 182 enzymes: SgfI *
March 22, 2012 18:35 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 766 890 1124 1725 2048 2123 2337
Size: 221 545 124 234 601 323 75 214
Fragments arranged by size:
601 545 323 234 221 214 124 75
AgeI A'CCGG_T
Cuts at: 0 265 2337
Size: 265 2072
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 444 2337
Size: 444 1893
AlwNI CAG_nnn'CTG
Cuts at: 0 174 411 549 741 1887 2337
Size: 174 237 138 192 1146 450
Fragments arranged by size:
1146 450 237 192 174 138
ApoI r'AATT_y
Cuts at: 0 142 271 1108 1253 1377 2337
Size: 142 129 837 145 124 960
Fragments arranged by size:
960 837 145 142 129 124
AseI AT'TA_AT
Cuts at: 0 1456 2337
Size: 1456 881
AvaI C'yCGr_G
Cuts at: 0 1663 2337
Size: 1663 674
BanII G_rGCy'C
Cuts at: 0 497 2337
Size: 497 1840
BbsI GAAGACnn'nnnn_
Cuts at: 0 209 658 2337
Size: 209 449 1679
Fragments arranged by size:
1679 449 209
BciVI GTATCCnnnnn_n'
Cuts at: 0 1359 2337
Size: 1359 978
BclI T'GATC_A
Cuts at: 0 1503 2337
Size: 1503 834
BlpI GC'TnA_GC
Cuts at: 0 404 2337
Size: 404 1933
Bme1580I G_kGCm'C
Cuts at: 0 1785 2337
Size: 1785 552
BmrI ACTGGGnnnn_n'
Cuts at: 0 1599 2337
Size: 1599 738
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 892 2337
Size: 892 1445
BsaAI yAC'GTr
Cuts at: 0 258 2337
Size: 258 2079
BsaWI w'CCGG_w
Cuts at: 0 265 2337
Size: 265 2072
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 997 1027 2337
Size: 997 30 1310
Fragments arranged by size:
1310 997 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 109 661 2337
Size: 109 552 1676
Fragments arranged by size:
1676 552 109
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1873 2337
Size: 1873 464
BsiHKAI G_wGCw'C
Cuts at: 0 405 497 908 2337
Size: 405 92 411 1429
Fragments arranged by size:
1429 411 405 92
BsmI GAATG_Cn'
Cuts at: 0 1765 2337
Size: 1765 572
Bsp1286I G_dGCh'C
Cuts at: 0 405 497 908 1785 2337
Size: 405 92 411 877 552
Fragments arranged by size:
877 552 411 405 92
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 221 1872 2337
Size: 221 1651 465
Fragments arranged by size:
1651 465 221
BsrDI GCAATG_nn'
Cuts at: 0 1640 2337
Size: 1640 697
BsrFI r'CCGG_y
Cuts at: 0 265 2337
Size: 265 2072
BsrGI T'GTAC_A
Cuts at: 0 1637 2337
Size: 1637 700
BssHII G'CGCG_C
Cuts at: 0 164 2337
Size: 164 2173
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1887 2337
Size: 1887 450
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 2337
Size: 591 1746
BstYI r'GATC_y
Cuts at: 0 54 249 1725 2337
Size: 54 195 1476 612
Fragments arranged by size:
1476 612 195 54
Bsu36I CC'TnA_GG
Cuts at: 0 757 2337
Size: 757 1580
BtsI GCAGTG_nn'
Cuts at: 0 347 1709 2337
Size: 347 1362 628
Fragments arranged by size:
1362 628 347
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 336 371 2337
Size: 336 35 1966
Fragments arranged by size:
1966 336 35
DraI TTT'AAA
Cuts at: 0 467 1142 2337
Size: 467 675 1195
Fragments arranged by size:
1195 675 467
DraIII CAC_nnn'GTG
Cuts at: 0 1371 2337
Size: 1371 966
DrdI GACnn_nn'nnGTC
Cuts at: 0 310 2337
Size: 310 2027
EarI CTCTTCn'nnn_
Cuts at: 0 410 530 1079 1841 2126 2262 2304 2337
Size: 410 120 549 762 285 136 42 33
Fragments arranged by size:
762 549 410 285 136 120 42 33
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 232 2337
Size: 232 2105
EcoICRI GAG'CTC
Cuts at: 0 495 2337
Size: 495 1842
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 221 766 890 892 1124 1725 2048 2123
Size: 221 545 124 2 232 601 323 75
Cuts at: 2123 2337
Size: 214
Fragments arranged by size:
601 545 323 232 221 214 124 75
2
EcoO109I rG'GnC_Cy
Cuts at: 0 62 171 2077 2236 2337
Size: 62 109 1906 159 101
Fragments arranged by size:
1906 159 109 101 62
EcoRI G'AATT_C
Cuts at: 0 142 2337
Size: 142 2195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1813 2337
Size: 1813 524
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 181 213 983 997 1015 1029 2005 2037
Size: 181 32 770 14 18 14 976 32
Cuts at: 2037 2337
Size: 300
Fragments arranged by size:
976 770 300 181 32 32 18 14
14
HindIII A'AGCT_T
Cuts at: 0 718 2273 2337
Size: 718 1555 64
Fragments arranged by size:
1555 718 64
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 275 1078 2337
Size: 275 803 1259
Fragments arranged by size:
1259 803 275
MslI CAynn'nnrTG
Cuts at: 0 426 2337
Size: 426 1911
MspA1I CmG'CkG
Cuts at: 0 765 2248 2317 2337
Size: 765 1483 69 20
Fragments arranged by size:
1483 765 69 20
NspI r_CATG'y
Cuts at: 0 159 1430 2337
Size: 159 1271 907
Fragments arranged by size:
1271 907 159
PfoI T'CCnGG_A
Cuts at: 0 34 223 2337
Size: 34 189 2114
Fragments arranged by size:
2114 189 34
PpuMI rG'GwC_Cy
Cuts at: 0 62 2077 2236 2337
Size: 62 2015 159 101
Fragments arranged by size:
2015 159 101 62
PstI C_TGCA'G
Cuts at: 0 1863 2337
Size: 1863 474
PvuII CAG'CTG
Cuts at: 0 2248 2317 2337
Size: 2248 69 20
Fragments arranged by size:
2248 69 20
SacI G_AGCT'C
Cuts at: 0 497 2337
Size: 497 1840
SanDI GG'GwC_CC
Cuts at: 0 62 2337
Size: 62 2275
SapI GCTCTTCn'nnn_
Cuts at: 0 2304 2337
Size: 2304 33
SfcI C'TryA_G
Cuts at: 0 1154 1859 2337
Size: 1154 705 478
Fragments arranged by size:
1154 705 478
SphI G_CATG'C
Cuts at: 0 159 2337
Size: 159 2178
SspI AAT'ATT
Cuts at: 0 473 2337
Size: 473 1864
StyI C'CwwG_G
Cuts at: 0 528 2337
Size: 528 1809
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 681 2337
Size: 681 1656
TatI w'GTAC_w
Cuts at: 0 134 1338 1637 2337
Size: 134 1204 299 700
Fragments arranged by size:
1204 700 299 134
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 219 251 2337
Size: 219 32 2086
Fragments arranged by size:
2086 219 32
XbaI T'CTAG_A
Cuts at: 0 921 2337
Size: 921 1416
XmnI GAAnn'nnTTC
Cuts at: 0 1543 2135 2337
Size: 1543 592 202
Fragments arranged by size:
1543 592 202
Enzymes that do cut:
AcuI AgeI AlfI AlwNI ApoI AseI AvaI BanII
BbsI BciVI BclI BlpI Bme1580I BmrI BpmI BsaAI
BsaWI BsaXI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BsrGI BssHII BstAPI BstXI BstYI Bsu36I
BtsI CspCI DraI DraIII DrdI EarI EciI EcoICRI
Eco57MI EcoO109I EcoRI FalI Hin4I HindIII MmeI MslI
MspA1I NspI PfoI PpuMI PstI PvuII SacI SanDI
SapI SfcI SphI SspI StyI TaqII TatI TstI
XbaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvrII
BaeI BamHI BanI BbeI BbvCI BcgI BfrBI BglI
BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrBI
BssSI BstBI BstEII BstZ17I BtgI BtgZI ClaI EaeI
EagI EcoNI EcoRV FseI FspI FspAI HaeII HincII
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI StuI SwaI Tth111I XcmI XhoI
XmaI ZraI