################################## # NO CUT ENZYMES ( 151 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwI AlwNI Aor51HI ApaI ApaLI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BalI BamHI BanI BbeI BbrPI BcgI BciVI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaHI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstXI BstYI BstZ17I CelII Cfr10I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRI EcoRV EcoT22I FauI FseI FspI HaeII HapII Hin1I HpaI HpaII KasI KpnI KspI MfeI MflI MluI MluNI MroI MscI MspA1I MspI MunI MvnI NaeI NarI NciI NgoMIV NheI NlaIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PstI PvuI PvuII RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI SwaI Van91I XbaI XhoI XhoII XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AspHI 667 GAGCAC G[AT]GC[AT]^C AspI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 161 CCCGAG C^[CT]CG[GA]G AvaII 12 GGTCC G^G[AT]CC BanII 1035 GGGCTC G[GA]GC[CT]^C BbvCI 544 GCTGAGG CCTCAGC(-5/-2) BclI 836 TGATCA T^GATCA BsaBI 837 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseRI 338 CTCCTC GAGGAG(10/8) BsgI 349 GTGCAG GTGCAG(16/14) BsiHKAI 667 GAGCAC G[AT]GC[AT]^C BsoBI 161 CCCGAG C^[CT]CG[GA]G BspLU11I 788 ACATGT A^CATGT BsrDI 506 GCAATG GCAATG(2/0) CfoI 1150 GCGC GCG^C Cfr13I 12 GGTCC G^G[ACGT]CC DraI 883 TTTAAA TTT^AAA DraII 11 AGGTCCT [GA]G^G[ACGT]CC[CT] EaeI 2 TGGCCG [CT]^GGCC[GA] EcoO109I 11 AGGTCCT [GA]G^G[ACGT]CC[CT] EcoT14I 47 CCATGG C^C[AT][AT]GG FbaI 836 TGATCA T^GATCA HgaI 255 GACGC GACGC(5/10) HhaI 1150 GCGC GCG^C HinP1I 1150 GCGC G^CGC HincII 550 GTTGAC GT[CT]^[GA]AC HindII 550 GTTGAC GT[CT]^[GA]AC HindIII 400 AAGCTT A^AGCTT MamI 837 GATCATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NcoI 47 CCATGG C^CATGG NdeI 1066 CATATG CA^TATG PflFI 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 11 AGGTCCT [GA]G^G[AT]CC[CT] SapI 992 GCTCTTC GCTCTTC(1/4) Sau96I 12 GGTCC G^G[ACGT]CC ScaI 318 AGTACT AGT^ACT SmlI 311 CTCAAG C^T[CT][GA]AG SphI 202 GCATGC GCATG^C StuI 848 AGGCCT AGG^CCT StyI 47 CCATGG C^C[AT][AT]GG TaqI 1119 TCGA T^CGA TfiI 889 GATTC G^A[AT]TC Tth111I 7 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 1119 TCGA T^CGA XcmI 846 CCAGGCCTGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 484 ACACGT A^C[GA][CT]GT 788 ACATGT AocI 340 CCTCAGG CC^T[ACGT]AGG 377 CCTCAGG BbsI 235 GAAGAC GAAGAC(2/6) 1052 GAAGAC BbvI 1134 GCAGC GCAGC(8/12) 573 GCTGC BfaI 412 CTAG C^TAG 530 CTAG BpmI 539 CTGGAG CTGGAG(16/14) 643 CTGGAG BpuAI 235 GAAGAC GAAGAC(2/6) 1052 GAAGAC BsmAI 1020 GAGAC GTCTC(1/5) 705 GTCTC BsrI 75 ACTGG ACTGG(1/-1) 538 ACTGG Bsu36I 340 CCTCAGG CC^T[ACGT]AGG 377 CCTCAGG Cac8I 202 GCATGC GC[ACGT]^[ACGT]GC 850 GCCTGC DpnII 274 GATC ^GATC 837 GATC DsaI 47 CCATGG C^C[GA][CT]GG 855 CCGTGG Eco81I 340 CCTCAGG CC^T[ACGT]AGG 377 CCTCAGG HaeIII 3 GGCC GG^CC 849 GGCC MaeI 412 CTAG C^TAG 530 CTAG MboI 274 GATC ^GATC 837 GATC MwoI 206 GCCAAATTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 337 GCTCCTCAGGC NdeII 274 GATC ^GATC 837 GATC NspI 202 GCATGC [GA]CATG^[CT] 788 ACATGT Sau3AI 274 GATC ^GATC 837 GATC TseI 573 GCTGC G^C[AT]GC 1134 GCAGC Tsp45I 860 GTGAC ^GT[GC]AC 1174 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 39 enzymes) # ######################################### AciI AcsI AfaI AluI ApoI BmyI BsaJI BsiYI BslI BsmFI Bsp1286I BssKI BstF5I BstNI DdeI EarI EcoRII Fnu4HI FokI HinfI HphI ItaI Ksp632I MaeII MaeIII MboII MnlI MseI MslI MvaI NlaIII PleI RsaI ScrFI SfaNI SfcI Tru9I Tsp509I TspRI