Restriction map for
pFN21AE5629
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# NO CUT ENZYMES ( 124 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AvaI AvrII BbrPI BclI
BcnI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaAI BsaBI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmFI BsoBI BspDI BspEI
BspLU11I BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI DraIII
DrdI DsaI EagI EclXI Eco52I
EcoNI EcoO65I EcoRI EcoRV FbaI
FseI HincII HindII HindIII HpaI
KpnI KspI MaeI MaeII MamI
MfeI MluI MroI MunI NaeI
NciI NcoI NgoMIV NheI NotI
NruI NspI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 60 enzymes) #
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AcyI 926 GGCGCC G[GA]^CG[CT]C
AfaI 775 GTAC GT^AC
AhdI 42 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 1356 CAGCATCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 680 CCTTAGG CC^T[ACGT]AGG
Aor51HI 119 AGCGCT AGC^GCT
AspEI 42 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 660 TGCGCA TGC^GCA
BalI 979 TGGCCA TGG^CCA
BamHI 782 GGATCC G^GATCC
BanI 926 GGCGCC G^G[CT][GA]CC
BbeI 926 GGCGCC GGCGC^C
BbvCI 937 CCTCAGC CCTCAGC(-5/-2)
BcgI 471 CGATGGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1167 GGATAC GTATCC(6/5)
BsaHI 926 GGCGCC G[GA]^CG[CT]C
BsgI 1148 GTGCAG GTGCAG(16/14)
Bsp1407I 774 TGTACA T^GTACA
BspMI 942 GCAGGT ACCTGC(4/8)
BsrDI 477 CATTGC GCAATG(2/0)
BsrFI 739 ACCGGC [GA]^CCGG[CT]
BsrGI 774 TGTACA T^GTACA
BstYI 782 GGATCC [GA]^GATC[CT]
Bsu36I 680 CCTTAGG CC^T[ACGT]AGG
Cfr10I 739 ACCGGC [GA]^CCGG[CT]
DraI 907 TTTAAA TTT^AAA
Eam1105I 42 GACTCATTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 28 CTCTTC CTCTTC(1/4)
Eco47III 119 AGCGCT AGC^GCT
Eco81I 680 CCTTAGG CC^T[ACGT]AGG
EcoT14I 698 CCAAGG C^C[AT][AT]GG
EcoT22I 1 ATGCAT ATGCA^T
FspI 660 TGCGCA TGC^GCA
HapII 740 CCGG C^CGG
HgaI 1382 GCGTC GACGC(5/10)
Hin1I 926 GGCGCC G[GA]^CG[CT]C
HpaII 740 CCGG C^CGG
KasI 926 GGCGCC G^GCGCC
Ksp632I 28 CTCTTC CTCTTC(1/4)
MflI 782 GGATCC [GA]^GATC[CT]
MluNI 979 TGGCCA TGG^CCA
MscI 979 TGGCCA TGG^CCA
MslI 598 CATTTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1151 CAGCTG C[AC]G^C[GT]G
MspI 740 CCGG C^CGG
MwoI 933 GCATCCTCAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NarI 926 GGCGCC GG^CGCC
NdeI 1010 CATATG CA^TATG
NsiI 1 ATGCAT ATGCA^T
PvuII 1151 CAGCTG CAG^CTG
RsaI 775 GTAC GT^AC
SexAI 1199 ACCAGGT A^CC[AT]GGT
SfcI 357 CTACAG C^T[GA][CT]AG
SfoI 926 GGCGCC GGC^GCC
SmlI 33 CTTGAG C^T[CT][GA]AG
SspBI 774 TGTACA T^GTACA
StyI 698 CCAAGG C^C[AT][AT]GG
TspRI 69 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 786 CCAGTCTCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 782 GGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 156 CGCG CG^CG
1381 CGCG
AlwI 783 GATCC GGATC(4/5)
782 GGATC
ApaI 53 GGGCCC GGGCC^C
538 GGGCCC
AspHI 710 GAGCAC G[AT]GC[AT]^C
1015 GAGCAC
BsiHKAI 710 GAGCAC G[AT]GC[AT]^C
1015 GAGCAC
BsmI 174 GAATGC GAATGC(1/-1)
409 GCATTC
BstUI 156 CGCG CG^CG
1381 CGCG
EaeI 672 CGGCCA [CT]^GGCC[GA]
979 TGGCCA
FauI 157 GCGGG CCCGC(4/6)
287 GCGGG
HaeII 119 AGCGCT [GA]GCGC^[CT]
926 GGCGCC
MvnI 156 CGCG CG^CG
1381 CGCG
PpuMI 11 GGGTCCT [GA]G^G[AT]CC[CT]
701 AGGACCC
TaqI 324 TCGA T^CGA
401 TCGA
TfiI 161 GAATC G^A[AT]TC
917 GATTC
Tsp45I 235 GTGAC ^GT[GC]AC
851 GTGAC
TthHB8I 324 TCGA T^CGA
401 TCGA
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# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AluI ApoI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BseRI BsiYI BslI
BsmAI Bsp1286I BspHI BsrI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI FokI HaeIII HhaI HinP1I
HinfI HphI ItaI MaeIII MboI
MboII MnlI MseI MvaI NdeII
NlaIII NlaIV PflMI PleI RcaI
Sau3AI Sau96I ScrFI SfaNI Tru9I
TseI Tsp509I Van91I