Restriction map for
pFN21AE5629
(Circular) (Six-base) MAPSORT of: pFN21AE5629.seq Check: 920 from: 1 to: 6118
pFN21AE5629 6118 bp
With 182 enzymes: SgfI *
December 8, 2014 13:40 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3928 278
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3461 1937
Size: 1524 4594
Acc65I G'GTAC_C
Cuts at: 3439 3439
Size: 6118
AclI AA'CG_TT
Cuts at: 1563 4245 4618 1563
Size: 2682 373 3063
Fragments arranged by size:
3063 2682 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2192 2421 3034 4181 5565 5833 1632
Size: 560 229 613 1147 1384 268 1917
Fragments arranged by size:
1917 1384 1147 613 560 268 229
AfeI AGC'GCT
Cuts at: 2126 2126
Size: 6118
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5921
AflIII A'CryG_T
Cuts at: 1203 5017 5994 1203
Size: 3814 977 1327
Fragments arranged by size:
3814 1327 977
AgeI A'CCGG_T
Cuts at: 4949 4949
Size: 6118
AhdI GACnn_n'nnGTC
Cuts at: 2052 4847 2052
Size: 2795 3323
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 6118
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2970 1184
Size: 1786 4332
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2617 2649 2617
Size: 32 6086
AlwNI CAG_nnn'CTG
Cuts at: 3366 3861 5433 3366
Size: 495 1572 4051
Fragments arranged by size:
4051 1572 495
ApaI G_GGCC'C
Cuts at: 2062 2547 2062
Size: 485 5633
ApaLI G'TGCA_C
Cuts at: 4175 5331 4175
Size: 1156 4962
ApoI r'AATT_y
Cuts at: 1493 2353 2458 3062 3400 3427 3653 3805 5914
Size: 860 105 604 338 27 226 152 2109
Cuts at: 5914 1493
Size: 1697
Fragments arranged by size:
2109 1697 860 604 338 226 152 105
27
AseI AT'TA_AT
Cuts at: 160 1032 4672 160
Size: 872 3640 1606
Fragments arranged by size:
3640 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
AvaI C'yCGr_G
Cuts at: 1870 1943 3443 1870
Size: 73 1500 4545
Fragments arranged by size:
4545 1500 73
BamHI G'GATC_C
Cuts at: 2787 3448 3815 2787
Size: 661 367 5090
Fragments arranged by size:
5090 661 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2931 3439 4894 618
Size: 325 217 627 1144 508 1455 1842
Fragments arranged by size:
1842 1455 1144 627 508 325 217
BanII G_rGCy'C
Cuts at: 1391 2062 2547 3073 3437 1391
Size: 671 485 526 364 4072
Fragments arranged by size:
4072 671 526 485 364
BbeI G_GCGC'C
Cuts at: 2935 2935
Size: 6118
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 2112 2823 3119 3276 928
Size: 971 213 711 296 157 3770
Fragments arranged by size:
3770 971 711 296 213 157
BbvCI CC'TCA_GC
Cuts at: 2943 2943
Size: 6118
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 1987 2021 2465 2499 4309 4343 1903
Size: 34 50 34 444 34 1810 34 3678
Fragments arranged by size:
3678 1810 444 50 34 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3166 4010 5220 3166
Size: 844 1210 4064
Fragments arranged by size:
4064 1210 844
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6118
BfrBI ATG'CAT
Cuts at: 2008 2008
Size: 6118
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4729 136
Size: 107 122 71 631 605 3057 1525
Fragments arranged by size:
3057 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6113 6113
Size: 6118
BlpI GC'TnA_GC
Cuts at: 3535 3535
Size: 6118
Bme1580I G_kGCm'C
Cuts at: 1790 2062 2547 4179 5335 1790
Size: 272 485 1632 1156 2573
Fragments arranged by size:
2573 1632 1156 485 272
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6118
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2067 3131 3849
Size: 416 53 83 399 24 650 1064 718
Cuts at: 3849 4807 442
Size: 958 1753
Fragments arranged by size:
1753 1064 958 718 650 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2064 2064
Size: 6118
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2096 2733 3232 3388 4778 1239
Size: 857 637 499 156 1390 2579
Fragments arranged by size:
2579 1390 857 637 499 156
Bpu10I CC'TnA_GC
Cuts at: 2943 2943
Size: 6118
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2059 4243 5108 5406 2059
Size: 2184 865 298 2771
Fragments arranged by size:
2771 2184 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4781 882
Size: 617 3282 2219
Fragments arranged by size:
3282 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3909 493
Size: 711 2705 2702
Fragments arranged by size:
2705 2702 711
BsaBI GATnn'nnATC
Cuts at: 6112 6112
Size: 6118
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2932 3925 4307 4942
Size: 53 83 186 936 1399 993 382 635
Cuts at: 4942 275
Size: 1451
Fragments arranged by size:
1451 1399 993 936 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 4551 4949 5223 5370 5781 4551
Size: 398 274 147 411 4888
Fragments arranged by size:
4888 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3361 3391 1178
Size: 30 165 30 1958 30 3905
Fragments arranged by size:
3905 1958 165 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2442 2607 2910 1186
Size: 1256 165 303 4394
Fragments arranged by size:
4394 1256 303 165
BseYI C'CCAG_C
Cuts at: 5321 5817 5321
Size: 496 5622
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3174 1163
Size: 573 1438 4107
Fragments arranged by size:
4107 1438 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 3568 4329 5357 1540
Size: 275 186 1567 761 1028 2301
Fragments arranged by size:
2301 1567 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2719 3024 4179 4264 5335 2719
Size: 305 1155 85 1071 3502
Fragments arranged by size:
3502 1155 1071 305 85
BsmI GAATG_Cn'
Cuts at: 2185 2414 3629 3722 2185
Size: 229 1215 93 4581
Fragments arranged by size:
4581 1215 229 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2062 2547 2719 3024 3073 3437 4179
Size: 399 272 485 172 305 49 364 742
Cuts at: 4179 4264 5335 1391
Size: 85 1071 2174
Fragments arranged by size:
2174 1071 742 485 399 364 305 272
172 85 49
BspEI T'CCGG_A
Cuts at: 5781 5781
Size: 6118
BspHI T'CATG_A
Cuts at: 2274 2493 3031 4007 5737 2274
Size: 219 538 976 1730 2655
Fragments arranged by size:
2655 1730 976 538 219
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2938 3473 844
Size: 2094 535 3489
Fragments arranged by size:
3489 2094 535
BsrBI CCG'CTC
Cuts at: 1394 1823 4005 1394
Size: 429 2182 3507
Fragments arranged by size:
3507 2182 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2481 4613 4787 65
Size: 1183 1233 2132 174 1396
Fragments arranged by size:
2132 1396 1233 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2744 4762 4949 1700
Size: 110 934 2018 187 2869
Fragments arranged by size:
2869 2018 934 187 110
BsrGI T'GTAC_A
Cuts at: 96 2779 96
Size: 2683 3435
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6118
BssSI C'ACGA_G
Cuts at: 4178 5190 4178
Size: 1012 5106
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 6118
BstBI TT'CG_AA
Cuts at: 4922 4922
Size: 6118
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6118
BstYI r'GATC_y
Cuts at: 1972 2787 3448 3815 4200 4217 5669 5755 5767
Size: 815 661 367 385 17 1452 86 12
Cuts at: 5767 6113 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 815 661 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2686 2686
Size: 6118
BtgI C'CryG_G
Cuts at: 513 1065 513
Size: 552 5566
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5379
Fragments arranged by size:
5379 683 56
BtsI GCAGTG_nn'
Cuts at: 3634 4428 4448 3634
Size: 794 20 5304
Fragments arranged by size:
5304 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6118
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6083
DraI TTT'AAA
Cuts at: 2914 3422 2914
Size: 508 5610
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5125 809
Size: 507 3809 1802
Fragments arranged by size:
3809 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2677 2984 3565
Size: 54 1425 50 275 18 847 307 581
Cuts at: 3565 4454 6083 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 847 581 307 275 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 3565 1537
Size: 275 1753 4090
Fragments arranged by size:
4090 1753 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2039 4048 1353
Size: 686 2009 3423
Fragments arranged by size:
3423 2009 686
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4705 5079 5225 387
Size: 1064 138 3116 374 146 1280
Fragments arranged by size:
3116 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2096 2192 2421 2733 3034 3232 3388
Size: 393 464 96 229 312 301 198 156
Cuts at: 3388 4181 4778 5565 5833 1239
Size: 793 597 787 268 1524
Fragments arranged by size:
1524 793 787 597 464 393 312 301
268 229 198 156 96
EcoO109I rG'GnC_Cy
Cuts at: 2017 2059 2544 2707 2017
Size: 42 485 163 5428
Fragments arranged by size:
5428 485 163 42
EcoRI G'AATT_C
Cuts at: 3427 3805 3427
Size: 378 5740
FspI TGC'GCA
Cuts at: 2667 4624 2667
Size: 1957 4161
HaeII r_GCGC'y
Cuts at: 1678 2128 2935 5265 1678
Size: 450 807 2330 2531
Fragments arranged by size:
2531 2330 807 450
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2038 2070 2081 2113 2531
Size: 32 135 32 466 32 11 32 418
Cuts at: 2531 2563 2779 2811 4759 4791 4833 4865 1373
Size: 32 216 32 1948 32 42 32 2626
Fragments arranged by size:
2626 1948 466 418 216 135 42 32
32 32 32 32 32 32 32 11
HincII GTy'rAC
Cuts at: 669 1657 1938 3462 3708 669
Size: 988 281 1524 246 3079
Fragments arranged by size:
3079 1524 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6118
HpaI GTT'AAC
Cuts at: 3708 3708
Size: 6118
KasI G'GCGC_C
Cuts at: 2931 2931
Size: 6118
KpnI G_GTAC'C
Cuts at: 3443 3443
Size: 6118
MfeI C'AATT_G
Cuts at: 3717 3717
Size: 6118
MluI A'CGCG_T
Cuts at: 5994 5994
Size: 6118
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5232 5416 1305
Size: 3927 184 2007
Fragments arranged by size:
3927 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2986 10
Size: 54 1425 343 1154 3142
Fragments arranged by size:
3142 1425 1154 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2607 4076 4435 4594 518
Size: 648 1441 1469 359 159 2042
Fragments arranged by size:
2042 1469 1441 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 3158 3534 4211 5359 5604 1606
Size: 1552 376 677 1148 245 2120
Fragments arranged by size:
2120 1552 1148 677 376 245
NarI GG'CG_CC
Cuts at: 2932 2932
Size: 6118
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5566
NdeI CA'TA_TG
Cuts at: 387 3016 387
Size: 2629 3489
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6118
NotI GC'GGCC_GC
Cuts at: 3565 3565
Size: 6118
NsiI A_TGCA'T
Cuts at: 2010 2010
Size: 6118
NspI r_CATG'y
Cuts at: 1132 1229 3476 5021 1132
Size: 97 2247 1545 2229
Fragments arranged by size:
2247 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 6118
PciI A'CATG_T
Cuts at: 5017 5017
Size: 6118
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2540 2588 3238 3284 3411 1108
Size: 1432 48 650 46 127 3815
Fragments arranged by size:
3815 1432 650 127 48 46
PfoI T'CCnGG_A
Cuts at: 2806 2806
Size: 6118
PmeI GTTT'AAAC
Cuts at: 3422 3422
Size: 6118
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4187 4219 5700 5732 4187
Size: 32 1481 32 4573
Fragments arranged by size:
4573 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2017 2707 2017
Size: 690 5428
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6118
PsiI TTA'TAA
Cuts at: 3688 3688
Size: 6118
PspOMI G'GGCC_C
Cuts at: 2058 2543 2058
Size: 485 5633
PstI C_TGCA'G
Cuts at: 830 3470 830
Size: 2640 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6118
PvuII CAG'CTG
Cuts at: 3158 3158
Size: 6118
SalI G'TCGA_C
Cuts at: 1936 3460 1936
Size: 1524 4594
SbfI CC_TGCA'GG
Cuts at: 3470 3470
Size: 6118
ScaI AGT'ACT
Cuts at: 4366 6078 4366
Size: 1712 4406
SexAI A'CCwGG_T
Cuts at: 3204 3204
Size: 6118
SfcI C'TryA_G
Cuts at: 826 1045 2362 3466 4601 5282 5473 826
Size: 219 1317 1104 1135 681 191 1471
Fragments arranged by size:
1471 1317 1135 1104 681 219 191
SfoI GGC'GCC
Cuts at: 2933 2933
Size: 6118
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6118
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6118
SmaI CCC'GGG
Cuts at: 1872 3445 1872
Size: 1573 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2038 4222 5123 5385 820
Size: 197 926 95 2184 901 262 1553
Fragments arranged by size:
2184 1553 926 901 262 197 95
SnaBI TAC'GTA
Cuts at: 493 3909 493
Size: 3416 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6118
SphI G_CATG'C
Cuts at: 1132 3476 1132
Size: 2344 3774
SspI AAT'ATT
Cuts at: 5 52 4042 5
Size: 47 3990 2081
Fragments arranged by size:
3990 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6118
StyI C'CwwG_G
Cuts at: 513 1065 2703 3557 513
Size: 552 1638 854 3074
Fragments arranged by size:
3074 1638 854 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2297 4145 4162 4315 4500 4945 325
Size: 1255 717 1848 17 153 185 445 1498
Fragments arranged by size:
1848 1498 1255 717 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2779 4364 6076
Size: 274 80 33 51 1443 802 1585 1712
Cuts at: 6076 96
Size: 138
Fragments arranged by size:
1712 1585 1443 802 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6078 6110 6078
Size: 32 6086
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 6118
XbaI T'CTAG_A
Cuts at: 3454 3454
Size: 6118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2798 2798
Size: 6118
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6118
XmaI C'CCGG_G
Cuts at: 1870 3443 1870
Size: 1573 4545
XmnI GAAnn'nnTTC
Cuts at: 4247 4247
Size: 6118
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3926 276
Size: 53 83 186 936 2392 2468
Fragments arranged by size:
2468 2392 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI MslI MspA1I
NarI NcoI NdeI NheI NotI NsiI NspI PasI
PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI
PspOMI PstI PvuI PvuII SalI SbfI ScaI SexAI
SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AscI AvrII BaeI BsiWI BsmBI BstXI BstZ17I
DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI
NgoMIV NruI PacI PmlI PspXI PsrI RsrII SacI
SacII SanDI SapI SfiI SrfI SwaI